Restriction map for pF1KB3458
 (Linear) (Six-base) MAPSORT of: KIBB3458.seq  Check: 8942  from: 1  to: 3351



KIBB3458.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     597    1043    1847    3351 
   Size:        597     446     804    1504

  Fragments arranged by size: 

                1504     804     597     446
 
AatII G_ACGT'C

Cuts at:      0    3248    3351 
   Size:       3248     103
 
AccI GT'mk_AC

Cuts at:      0    1072    3351 
   Size:       1072    2279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646    1240    1573    1905    3302    3316    3351 
   Size:        646     594     333     332    1397      14      35

  Fragments arranged by size: 

                1397     646     594     333     332      35      14
 
AflIII A'CryG_T

Cuts at:      0    2537    3351 
   Size:       2537     814
 
AleI CACnn'nnGTG

Cuts at:      0     442    3351 
   Size:        442    2909
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1535    3351 
   Size:       1535    1816
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     271     495     552     717     750     768    1410 
   Size:         15     256     224      57     165      33      18     642

Cuts at:   1410    2059    2766    2838    3351 
   Size:        649     707      72     513

  Fragments arranged by size: 

                 707     649     642     513     256     224     165      72
                  57      33      18      15
 
ApaI G_GGCC'C

Cuts at:      0    3164    3351 
   Size:       3164     187
 
ApoI r'AATT_y

Cuts at:      0     467     881    2241    2287    3351 
   Size:        467     414    1360      46    1064

  Fragments arranged by size: 

                1360    1064     467     414      46
 
AvaI C'yCGr_G

Cuts at:      0     237    1325    1782    1828    1911    2888    3351 
   Size:        237    1088     457      46      83     977     463

  Fragments arranged by size: 

                1088     977     463     457     237      83      46
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     958     991    3351 
   Size:        958      33    2360

  Fragments arranged by size: 

                2360     958      33
 
BamHI G'GATC_C

Cuts at:      0    1373    3351 
   Size:       1373    1978
 
BanI G'GyrC_C

Cuts at:      0     327     609     689     726    1715    1786    1954    2697 
   Size:        327     282      80      37     989      71     168     743

Cuts at:   2697    2866    2893    2926    3171    3351 
   Size:        169      27      33     245     180

  Fragments arranged by size: 

                 989     743     327     282     245     180     169     168
                  80      71      37      33      27
 
BanII G_rGCy'C

Cuts at:      0     347     528     575    1117    1145    1264    1315    1461 
   Size:        347     181      47     542      28     119      51     146

Cuts at:   1461    1682    1897    1912    1919    2959    3164    3351 
   Size:        221     215      15       7    1040     205     187

  Fragments arranged by size: 

                1040     542     347     221     215     205     187     181
                 146     119      51      47      28      15       7
 
BbeI G_GCGC'C

Cuts at:      0    1790    1958    3175    3351 
   Size:       1790     168    1217     176

  Fragments arranged by size: 

                1790    1217     176     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1228    3024    3351 
   Size:       1228    1796     327

  Fragments arranged by size: 

                1796    1228     327
 
BbvCI CC'TCA_GC

Cuts at:      0    1397    3351 
   Size:       1397    1954
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     865     899    3351 
   Size:        865      34    2452

  Fragments arranged by size: 

                2452     865      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     225    3351 
   Size:        225    3126
 
BclI T'GATC_A

Cuts at:      0    3206    3312    3351 
   Size:       3206     106      39

  Fragments arranged by size: 

                3206     106      39
 
BglI GCCn_nnn'nGGC

Cuts at:      0     145    3351 
   Size:        145    3206
 
BglII A'GATC_T

Cuts at:      0    1317    3351 
   Size:       1317    2034
 
BlpI GC'TnA_GC

Cuts at:      0     341    1258    1891    3351 
   Size:        341     917     633    1460

  Fragments arranged by size: 

                1460     917     633     341
 
Bme1580I G_kGCm'C

Cuts at:      0     330     614     692    1667    2869    3164    3351 
   Size:        330     284      78     975    1202     295     187

  Fragments arranged by size: 

                1202     975     330     295     284     187      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1119    1361    1412    3351 
   Size:       1119     242      51    1939

  Fragments arranged by size: 

                1939    1119     242      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2081    2200    3351 
   Size:       2081     119    1151

  Fragments arranged by size: 

                2081    1151     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     722    1397    3351 
   Size:        722     675    1954

  Fragments arranged by size: 

                1954     722     675
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1669    2124    3351 
   Size:        158    1511     455    1227

  Fragments arranged by size: 

                1511    1227     455     158
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1295    3351 
   Size:       1295    2056
 
BsaAI yAC'GTr

Cuts at:      0     292    2540    3351 
   Size:        292    2248     811

  Fragments arranged by size: 

                2248     811     292
 
BsaHI Gr'CG_yC

Cuts at:      0      80    1787    1955    1978    1987    3172    3245    3351 
   Size:         80    1707     168      23       9    1185      73     106

  Fragments arranged by size: 

                1707    1185     168     106      80      73      23       9
            
 
BsaWI w'CCGG_w

Cuts at:      0     389    2573    3351 
   Size:        389    2184     778

  Fragments arranged by size: 

                2184     778     389
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385    3351 
   Size:        355      30    2966

  Fragments arranged by size: 

                2966     355      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1690    2650    2653    2656    2659    2662    3351 
   Size:       1690     960       3       3       3       3     689

  Fragments arranged by size: 

                1690     960     689       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     576     614    1145    1190    1305    1460    2559    3351 
   Size:        576      38     531      45     115     155    1099     792

  Fragments arranged by size: 

                1099     792     576     531     155     115      45      38
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     754    1714    3351 
   Size:        481     273     960    1637

  Fragments arranged by size: 

                1637     960     481     273
 
BsiEI CG_ry'CG

Cuts at:      0    1723    3351 
   Size:       1723    1628
 
BsiHKAI G_wGCw'C

Cuts at:      0     540    1264    1315    2959    3351 
   Size:        540     724      51    1644     392

  Fragments arranged by size: 

                1644     724     540     392      51
 
BsmI GAATG_Cn'

Cuts at:      0     117    3351 
   Size:        117    3234
 
Bsp1286I G_dGCh'C

Cuts at:      0     330     347     528     540     575     614     692    1117 
   Size:        330      17     181      12      35      39      78     425

Cuts at:   1117    1145    1264    1315    1461    1667    1682    1897    1912 
   Size:         28     119      51     146     206      15     215      15

Cuts at:   1912    1919    2869    2959    3164    3351 
   Size:          7     950      90     205     187

  Fragments arranged by size: 

                 950     425     330     215     206     205     187     181
                 146     119      90      78      51      39      35      28
                  17      15      15      12       7
 
BspEI T'CCGG_A

Cuts at:      0     389    3351 
   Size:        389    2962
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     470     597    1043    1577    1847    3351 
   Size:        470     127     446     534     270    1504

  Fragments arranged by size: 

                1504     534     470     446     270     127
 
BsrDI GCAATG_nn'

Cuts at:      0    1932    2260    3351 
   Size:       1932     328    1091

  Fragments arranged by size: 

                1932    1091     328
 
BsrFI r'CCGG_y

Cuts at:      0     582    1718    2404    3351 
   Size:        582    1136     686     947

  Fragments arranged by size: 

                1136     947     686     582
 
BsrGI T'GTAC_A

Cuts at:      0      17    2484    3351 
   Size:         17    2467     867

  Fragments arranged by size: 

                2467     867      17
 
BssSI C'ACGA_G

Cuts at:      0    1858    3351 
   Size:       1858    1493
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     789    1005    1029    3351 
   Size:        789     216      24    2322

  Fragments arranged by size: 

                2322     789     216      24
 
BstEII G'GTnAC_C

Cuts at:      0       4    2046    2877    3351 
   Size:          4    2042     831     474

  Fragments arranged by size: 

                2042     831     474       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     106     292    1951    2511    3351 
   Size:         33      73     186    1659     560     840

  Fragments arranged by size: 

                1659     840     560     186      73      33
 
BstYI r'GATC_y

Cuts at:      0     386    1317    1373    3351 
   Size:        386     931      56    1978

  Fragments arranged by size: 

                1978     931     386      56
 
BstZ17I GTA'TAC

Cuts at:      0    1073    3351 
   Size:       1073    2278
 
Bsu36I CC'TnA_GG

Cuts at:      0     143    2778    3351 
   Size:        143    2635     573

  Fragments arranged by size: 

                2635     573     143
 
BtgI C'CryG_G

Cuts at:      0      39     566    1266    1599    1944    1996    2741    2859 
   Size:         39     527     700     333     345      52     745     118

Cuts at:   2859    3164    3351 
   Size:        305     187

  Fragments arranged by size: 

                 745     700     527     345     333     305     187     118
                  52      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1333    3351 
   Size:       1333    2018
 
BtsI GCAGTG_nn'

Cuts at:      0     518     748    3351 
   Size:        518     230    2603

  Fragments arranged by size: 

                2603     518     230
 
ClaI AT'CG_AT

Cuts at:      0    2027    3351 
   Size:       2027    1324
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3003    3038    3351 
   Size:       3003      35     313

  Fragments arranged by size: 

                3003     313      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3244    3247    3351 
   Size:       3244       3     104

  Fragments arranged by size: 

                3244     104       3
 
EaeI y'GGCC_r

Cuts at:      0     222     302    1485    1584    1720    1941    2191    2844 
   Size:        222      80    1183      99     136     221     250     653

Cuts at:   2844    3351 
   Size:        507

  Fragments arranged by size: 

                1183     653     507     250     222     221     136      99
                  80
 
EagI C'GGCC_G

Cuts at:      0    1720    3351 
   Size:       1720    1631
 
EarI CTCTTCn'nnn_

Cuts at:      0     665    1738    1924    2621    2681    3269    3351 
   Size:        665    1073     186     697      60     588      82

  Fragments arranged by size: 

                1073     697     665     588     186      82      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     143     532    2580    3351 
   Size:        143     389    2048     771

  Fragments arranged by size: 

                2048     771     389     143
 
EcoICRI GAG'CTC

Cuts at:      0    1262    1313    2957    3351 
   Size:       1262      51    1644     394

  Fragments arranged by size: 

                1644    1262     394      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646    1240    1573    1905    2081    2200    3302    3316 
   Size:        646     594     333     332     176     119    1102      14

Cuts at:   3316    3351 
   Size:         35

  Fragments arranged by size: 

                1102     646     594     333     332     176     119      35
                  14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     683    1289    1630    2152    3351 
   Size:        683     606     341     522    1199

  Fragments arranged by size: 

                1199     683     606     522     341
 
EcoO109I rG'GnC_Cy

Cuts at:      0     268     756    1059    1272    1279    1393    1469    1613 
   Size:        268     488     303     213       7     114      76     144

Cuts at:   1613    2056    2652    2763    3160    3351 
   Size:        443     596     111     397     191

  Fragments arranged by size: 

                 596     488     443     397     303     268     213     191
                 144     114     111      76       7
 
EcoRI G'AATT_C

Cuts at:      0     467    3351 
   Size:        467    2884
 
EcoRV GAT'ATC

Cuts at:      0    2139    3351 
   Size:       2139    1212
 
HaeII r_GCGC'y

Cuts at:      0    1790    1958    3175    3351 
   Size:       1790     168    1217     176

  Fragments arranged by size: 

                1790    1217     176     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1302    1334    2319    2351    2499    2531    2656    2688 
   Size:       1302      32     985      32     148      32     125      32

Cuts at:   2688    2882    2914    3351 
   Size:        194      32     437

  Fragments arranged by size: 

                1302     985     437     194     148     125      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     477    3351 
   Size:        477    2874
 
HindIII A'AGCT_T

Cuts at:      0     400    3351 
   Size:        400    2951
 
KasI G'GCGC_C

Cuts at:      0    1786    1954    3171    3351 
   Size:       1786     168    1217     180

  Fragments arranged by size: 

                1786    1217     180     168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2042    3351 
   Size:       2042    1309
 
MscI TGG'CCA

Cuts at:      0     224     304    1487    1586    2193    2846    3351 
   Size:        224      80    1183      99     607     653     505

  Fragments arranged by size: 

                1183     653     607     505     224      99      80
 
MslI CAynn'nnrTG

Cuts at:      0      44     104     442    1294    2178    3351 
   Size:         44      60     338     852     884    1173

  Fragments arranged by size: 

                1173     884     852     338      60      44
 
MspA1I CmG'CkG

Cuts at:      0      89     410     549     939     948    1101    1218    1410 
   Size:         89     321     139     390       9     153     117     192

Cuts at:   1410    1809    1968    2981    3127    3198    3351 
   Size:        399     159    1013     146      71     153

  Fragments arranged by size: 

                1013     399     390     321     192     159     153     153
                 146     139     117      89      71       9
 
NarI GG'CG_CC

Cuts at:      0    1787    1955    3172    3351 
   Size:       1787     168    1217     179

  Fragments arranged by size: 

                1787    1217     179     168
 
NcoI C'CATG_G

Cuts at:      0      39     566    1266    3351 
   Size:         39     527     700    2085

  Fragments arranged by size: 

                2085     700     527      39
 
NspI r_CATG'y

Cuts at:      0     783    1541    1593    3351 
   Size:        783     758      52    1758

  Fragments arranged by size: 

                1758     783     758      52
 
PasI CC'CwG_GG

Cuts at:      0    1110    2053    2766    3351 
   Size:       1110     943     713     585

  Fragments arranged by size: 

                1110     943     713     585
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      76    2059    3258    3351 
   Size:         76    1983    1199      93

  Fragments arranged by size: 

                1983    1199      93      76
 
PfoI T'CCnGG_A

Cuts at:      0    1844    3351 
   Size:       1844    1507
 
PmlI CAC'GTG

Cuts at:      0    2540    3351 
   Size:       2540     811
 
PpuMI rG'GwC_Cy

Cuts at:      0    1059    1272    1469    2652    3351 
   Size:       1059     213     197    1183     699

  Fragments arranged by size: 

                1183    1059     699     213     197
 
PshAI GACnn'nnGTC

Cuts at:      0    1327    3351 
   Size:       1327    2024
 
PspOMI G'GGCC_C

Cuts at:      0    3160    3351 
   Size:       3160     191
 
PspXI vC'TCGA_Gb

Cuts at:      0     237    3351 
   Size:        237    3114
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      43      75    3351 
   Size:         43      32    3276

  Fragments arranged by size: 

                3276      43      32
 
PstI C_TGCA'G

Cuts at:      0     755     944    1040    2762    3351 
   Size:        755     189      96    1722     589

  Fragments arranged by size: 

                1722     755     589     189      96
 
PvuII CAG'CTG

Cuts at:      0     549     939    1101    1218    1410    1809    1968    3127 
   Size:        549     390     162     117     192     399     159    1159

Cuts at:   3127    3198    3351 
   Size:         71     153

  Fragments arranged by size: 

                1159     549     399     390     192     162     159     153
                 117      71
 
SacI G_AGCT'C

Cuts at:      0    1264    1315    2959    3351 
   Size:       1264      51    1644     392

  Fragments arranged by size: 

                1644    1264     392      51
 
SanDI GG'GwC_CC

Cuts at:      0    1059    1469    3351 
   Size:       1059     410    1882

  Fragments arranged by size: 

                1882    1059     410
 
SapI GCTCTTCn'nnn_

Cuts at:      0     665    1924    3351 
   Size:        665    1259    1427

  Fragments arranged by size: 

                1427    1259     665
 
SbfI CC_TGCA'GG

Cuts at:      0    2762    3351 
   Size:       2762     589
 
ScaI AGT'ACT

Cuts at:      0    1129    2530    3351 
   Size:       1129    1401     821

  Fragments arranged by size: 

                1401    1129     821
 
SexAI A'CCwGG_T

Cuts at:      0    2873    3351 
   Size:       2873     478
 
SfcI C'TryA_G

Cuts at:      0     751     934     940    1036    1155    2072    2758    3351 
   Size:        751     183       6      96     119     917     686     593

  Fragments arranged by size: 

                 917     751     686     593     183     119      96       6
            
 
SfoI GGC'GCC

Cuts at:      0    1788    1956    3173    3351 
   Size:       1788     168    1217     178

  Fragments arranged by size: 

                1788    1217     178     168
 
SmaI CCC'GGG

Cuts at:      0    1327    3351 
   Size:       1327    2024
 
SmlI C'TyrA_G

Cuts at:      0     173     237    1684    2103    3351 
   Size:        173      64    1447     419    1248

  Fragments arranged by size: 

                1447    1248     419     173      64
 
StuI AGG'CCT

Cuts at:      0     148    3351 
   Size:        148    3203
 
StyI C'CwwG_G

Cuts at:      0      39     219     566     684    1266    2882    3351 
   Size:         39     180     347     118     582    1616     469

  Fragments arranged by size: 

                1616     582     469     347     180     118      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454    1870    1899    2944    3351 
   Size:        454    1416      29    1045     407

  Fragments arranged by size: 

                1416    1045     454     407      29
 
TatI w'GTAC_w

Cuts at:      0      17    1127    2484    2528    3351 
   Size:         17    1110    1357      44     823

  Fragments arranged by size: 

                1357    1110     823      44      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      65      97     572     604    1079    1111    1579    1611 
   Size:         65      32     475      32     475      32     468      32

Cuts at:   1611    2708    2740    3351 
   Size:       1097      32     611

  Fragments arranged by size: 

                1097     611     475     475     468      65      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3244    3247    3351 
   Size:       3244       3     104

  Fragments arranged by size: 

                3244     104       3
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      37    3222    3351 
   Size:         37    3185     129

  Fragments arranged by size: 

                3185     129      37
 
XhoI C'TCGA_G

Cuts at:      0     237    3351 
   Size:        237    3114
 
XmaI C'CCGG_G

Cuts at:      0    1325    3351 
   Size:       1325    2026
 
XmnI GAAnn'nnTTC

Cuts at:      0      55    1558    1708    3351 
   Size:         55    1503     150    1643

  Fragments arranged by size: 

                1643    1503     150      55
 
ZraI GAC'GTC

Cuts at:      0    3246    3351 
   Size:       3246     105
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI    PvuII     SacI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII    BfrBI    BmgBI     BmtI     BplI
    BsaBI    BsiWI    BsmBI    BspHI    BsrBI   BssHII    BstBI     DraI
   DraIII     FalI     FseI     FspI    FspAI     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI     PsiI     PvuI    RsrII    SacII
     SalI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI
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