(Linear) (Six-base) MAPSORT of: KIBB3457.seq  Check: 4197  from: 1  to: 528



KIBB3457.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     362     528 
   Size:        176     186     166

  Fragments arranged by size: 

                 186     176     166
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     455     528 
   Size:        455      73
 
ApoI r'AATT_y

Cuts at:      0     133     528 
   Size:        133     395
 
AvaI C'yCGr_G

Cuts at:      0     104     528 
   Size:        104     424
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     196     528 
   Size:        196     332
 
BbvCI CC'TCA_GC

Cuts at:      0     468     528 
   Size:        468      60
 
BclI T'GATC_A

Cuts at:      0      62     528 
   Size:         62     466
 
Bpu10I CC'TnA_GC

Cuts at:      0     468     528 
   Size:        468      60
 
BsaBI GATnn'nnATC

Cuts at:      0      26     528 
   Size:         26     502
 
BseYI C'CCAG_C

Cuts at:      0     452     528 
   Size:        452      76
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     528 
   Size:        111     417
 
BsmI GAATG_Cn'

Cuts at:      0     335     528 
   Size:        335     193
 
BstYI r'GATC_y

Cuts at:      0     493     528 
   Size:        493      35
 
EaeI y'GGCC_r

Cuts at:      0      92     528 
   Size:         92     436
 
EarI CTCTTCn'nnn_

Cuts at:      0      80     341     528 
   Size:         80     261     187

  Fragments arranged by size: 

                 261     187      80
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     362     528 
   Size:        176     186     166

  Fragments arranged by size: 

                 186     176     166
 
EcoRI G'AATT_C

Cuts at:      0     133     528 
   Size:        133     395
 
EcoRV GAT'ATC

Cuts at:      0     525     528 
   Size:        525       3
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      91     472     528 
   Size:         91     381      56

  Fragments arranged by size: 

                 381      91      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67     528 
   Size:         35      32     461

  Fragments arranged by size: 

                 461      35      32
 
MscI TGG'CCA

Cuts at:      0      94     528 
   Size:         94     434
 
NspI r_CATG'y

Cuts at:      0     211     528 
   Size:        211     317
 
SmlI C'TyrA_G

Cuts at:      0     104     528 
   Size:        104     424
 
StyI C'CwwG_G

Cuts at:      0     330     528 
   Size:        330     198
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318     528 
   Size:        318     210
 
XhoI C'TCGA_G

Cuts at:      0     104     528 
   Size:        104     424
 
XmnI GAAnn'nnTTC

Cuts at:      0     137     422     528 
   Size:        137     285     106

  Fragments arranged by size: 

                 285     137     106
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AvaI     BbsI    BbvCI     BclI   Bpu10I
    BsaBI    BseYI     BsgI     BsmI    BstYI     EaeI     EarI  Eco57MI
    EcoRI    EcoRV     FalI    Hin4I     MscI     NspI     SmlI     StyI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XmaI
     ZraI