Restriction map for
pF1KB3410
(Linear) (Six-base) MAPSORT of: KIBB3410.seq Check: 4590 from: 1 to: 2289
KIBB3410.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 612 2289
Size: 45 567 1677
Fragments arranged by size:
1677 567 45
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 409 505 1045 1230 1282 2289
Size: 268 141 96 540 185 52 1007
Fragments arranged by size:
1007 540 268 185 141 96 52
AflIII A'CryG_T
Cuts at: 0 1253 2114 2289
Size: 1253 861 175
Fragments arranged by size:
1253 861 175
AhdI GACnn_n'nnGTC
Cuts at: 0 408 2289
Size: 408 1881
AleI CACnn'nnGTG
Cuts at: 0 1960 2289
Size: 1960 329
AlwNI CAG_nnn'CTG
Cuts at: 0 27 60 180 201 354 663 717 780
Size: 27 33 120 21 153 309 54 63
Cuts at: 780 906 1023 1515 2044 2289
Size: 126 117 492 529 245
Fragments arranged by size:
529 492 309 245 153 126 120 117
63 54 33 27 21
ApaI G_GGCC'C
Cuts at: 0 800 2289
Size: 800 1489
ApaLI G'TGCA_C
Cuts at: 0 1084 2289
Size: 1084 1205
ApoI r'AATT_y
Cuts at: 0 1447 2158 2289
Size: 1447 711 131
Fragments arranged by size:
1447 711 131
AvaI C'yCGr_G
Cuts at: 0 802 2289
Size: 802 1487
AvrII C'CTAG_G
Cuts at: 0 1689 2289
Size: 1689 600
BamHI G'GATC_C
Cuts at: 0 11 392 542 2289
Size: 11 381 150 1747
Fragments arranged by size:
1747 381 150 11
BanI G'GyrC_C
Cuts at: 0 1228 1386 2289
Size: 1228 158 903
Fragments arranged by size:
1228 903 158
BanII G_rGCy'C
Cuts at: 0 165 580 800 2289
Size: 165 415 220 1489
Fragments arranged by size:
1489 415 220 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 1033 1932 2289
Size: 709 324 899 357
Fragments arranged by size:
899 709 357 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1773 1807 2289
Size: 1773 34 482
Fragments arranged by size:
1773 482 34
BfrBI ATG'CAT
Cuts at: 0 2032 2289
Size: 2032 257
BglI GCCn_nnn'nGGC
Cuts at: 0 565 2289
Size: 565 1724
BlpI GC'TnA_GC
Cuts at: 0 574 887 2289
Size: 574 313 1402
Fragments arranged by size:
1402 574 313
Bme1580I G_kGCm'C
Cuts at: 0 266 800 1088 1231 1242 2098 2289
Size: 266 534 288 143 11 856 191
Fragments arranged by size:
856 534 288 266 191 143 11
BmgBI CAC'GTC
Cuts at: 0 819 2289
Size: 819 1470
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 916 1569 2289
Size: 99 817 653 720
Fragments arranged by size:
817 720 653 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1959 2289
Size: 1959 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 265 328 595 652 1561 2289
Size: 202 63 63 267 57 909 728
Fragments arranged by size:
909 728 267 202 63 63 57
Bpu10I CC'TnA_GC
Cuts at: 0 1894 2289
Size: 1894 395
BsaI GGTCTCn'nnnn_
Cuts at: 0 2167 2289
Size: 2167 122
BsaAI yAC'GTr
Cuts at: 0 2063 2196 2289
Size: 2063 133 93
Fragments arranged by size:
2063 133 93
BsaHI Gr'CG_yC
Cuts at: 0 404 2289
Size: 404 1885
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 625 655 2289
Size: 625 30 1634
Fragments arranged by size:
1634 625 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 943 1004 1324 2289
Size: 460 483 61 320 965
Fragments arranged by size:
965 483 460 320 61
BseYI C'CCAG_C
Cuts at: 0 20 173 258 388 560 569 1884 2044
Size: 20 153 85 130 172 9 1315 160
Cuts at: 2044 2085 2289
Size: 41 204
Fragments arranged by size:
1315 204 172 160 153 130 85 41
20 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1194 1313 1483 2289
Size: 214 980 119 170 806
Fragments arranged by size:
980 806 214 170 119
BsiHKAI G_wGCw'C
Cuts at: 0 221 893 1088 2289
Size: 221 672 195 1201
Fragments arranged by size:
1201 672 221 195
BsiWI C'GTAC_G
Cuts at: 0 1053 2289
Size: 1053 1236
BsmI GAATG_Cn'
Cuts at: 0 2034 2289
Size: 2034 255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 29 595 2289
Size: 29 566 1694
Fragments arranged by size:
1694 566 29
Bsp1286I G_dGCh'C
Cuts at: 0 165 221 266 580 800 893 1088 1231
Size: 165 56 45 314 220 93 195 143
Cuts at: 1231 1242 2098 2289
Size: 11 856 191
Fragments arranged by size:
856 314 220 195 191 165 143 93
56 45 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 612 1730 2289
Size: 45 567 1118 559
Fragments arranged by size:
1118 567 559 45
BsrDI GCAATG_nn'
Cuts at: 0 1362 2289
Size: 1362 927
BsrFI r'CCGG_y
Cuts at: 0 866 1283 1880 2254 2289
Size: 866 417 597 374 35
Fragments arranged by size:
866 597 417 374 35
BsrGI T'GTAC_A
Cuts at: 0 338 2289
Size: 338 1951
BssSI C'ACGA_G
Cuts at: 0 1495 2289
Size: 1495 794
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 717 1172 2289
Size: 201 516 455 1117
Fragments arranged by size:
1117 516 455 201
BstEII G'GTnAC_C
Cuts at: 0 981 2289
Size: 981 1308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 741 2067 2184 2196 2289
Size: 141 600 1326 117 12 93
Fragments arranged by size:
1326 600 141 117 93 12
BstYI r'GATC_y
Cuts at: 0 11 392 542 875 1184 2289
Size: 11 381 150 333 309 1105
Fragments arranged by size:
1105 381 333 309 150 11
Bsu36I CC'TnA_GG
Cuts at: 0 538 1016 2289
Size: 538 478 1273
Fragments arranged by size:
1273 538 478
BtgI C'CryG_G
Cuts at: 0 124 1223 2177 2289
Size: 124 1099 954 112
Fragments arranged by size:
1099 954 124 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1776 2289
Size: 1776 513
BtsI GCAGTG_nn'
Cuts at: 0 661 1182 1627 2289
Size: 661 521 445 662
Fragments arranged by size:
662 661 521 445
DraIII CAC_nnn'GTG
Cuts at: 0 225 2289
Size: 225 2064
EaeI y'GGCC_r
Cuts at: 0 66 686 782 845 938 1417 2186 2289
Size: 66 620 96 63 93 479 769 103
Fragments arranged by size:
769 620 479 103 96 93 66 63
EarI CTCTTCn'nnn_
Cuts at: 0 1258 2289
Size: 1258 1031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 2289
Size: 229 2060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 265 268 328 409 505 595 652
Size: 202 63 3 60 81 96 90 57
Cuts at: 652 1045 1230 1282 1561 2289
Size: 393 185 52 279 728
Fragments arranged by size:
728 393 279 202 185 96 90 81
63 60 57 52 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 1483 2289
Size: 193 1290 806
Fragments arranged by size:
1290 806 193
EcoO109I rG'GnC_Cy
Cuts at: 0 186 797 1020 1476 1745 1868 1890 2076
Size: 186 611 223 456 269 123 22 186
Cuts at: 2076 2289
Size: 213
Fragments arranged by size:
611 456 269 223 213 186 186 123
22
EcoRI G'AATT_C
Cuts at: 0 2158 2289
Size: 2158 131
HaeII r_GCGC'y
Cuts at: 0 1438 2060 2289
Size: 1438 622 229
Fragments arranged by size:
1438 622 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 534 566 955 987 2289
Size: 394 32 108 32 389 32 1302
Fragments arranged by size:
1302 394 389 108 32 32 32
HincII GTy'rAC
Cuts at: 0 402 948 2289
Size: 402 546 1341
Fragments arranged by size:
1341 546 402
MfeI C'AATT_G
Cuts at: 0 372 2289
Size: 372 1917
MscI TGG'CCA
Cuts at: 0 2188 2289
Size: 2188 101
MslI CAynn'nnrTG
Cuts at: 0 1673 1909 1960 2065 2182 2289
Size: 1673 236 51 105 117 107
Fragments arranged by size:
1673 236 117 107 105 51
MspA1I CmG'CkG
Cuts at: 0 27 299 320 564 573 714 780 906
Size: 27 272 21 244 9 141 66 126
Cuts at: 906 1425 2089 2289
Size: 519 664 200
Fragments arranged by size:
664 519 272 244 200 141 126 66
27 21 9
NcoI C'CATG_G
Cuts at: 0 124 2177 2289
Size: 124 2053 112
Fragments arranged by size:
2053 124 112
NdeI CA'TA_TG
Cuts at: 0 291 2289
Size: 291 1998
NsiI A_TGCA'T
Cuts at: 0 2034 2289
Size: 2034 255
NspI r_CATG'y
Cuts at: 0 1257 2118 2289
Size: 1257 861 171
Fragments arranged by size:
1257 861 171
PciI A'CATG_T
Cuts at: 0 1253 2114 2289
Size: 1253 861 175
Fragments arranged by size:
1253 861 175
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1796 2289
Size: 180 1616 493
Fragments arranged by size:
1616 493 180
PfoI T'CCnGG_A
Cuts at: 0 179 740 2289
Size: 179 561 1549
Fragments arranged by size:
1549 561 179
PmlI CAC'GTG
Cuts at: 0 2196 2289
Size: 2196 93
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1324 1332 1351 1356 1383 2289
Size: 1300 24 8 19 5 27 906
Fragments arranged by size:
1300 906 27 24 19 8 5
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1745 1868 1890 2289
Size: 1020 725 123 22 399
Fragments arranged by size:
1020 725 399 123 22
PspOMI G'GGCC_C
Cuts at: 0 796 2289
Size: 796 1493
PstI C_TGCA'G
Cuts at: 0 32 206 497 668 713 2040 2289
Size: 32 174 291 171 45 1327 249
Fragments arranged by size:
1327 291 249 174 171 45 32
PvuII CAG'CTG
Cuts at: 0 27 564 573 714 780 906 1425 2089
Size: 27 537 9 141 66 126 519 664
Cuts at: 2089 2289
Size: 200
Fragments arranged by size:
664 537 519 200 141 126 66 27
9
ScaI AGT'ACT
Cuts at: 0 511 1951 2289
Size: 511 1440 338
Fragments arranged by size:
1440 511 338
SexAI A'CCwGG_T
Cuts at: 0 221 2289
Size: 221 2068
SfcI C'TryA_G
Cuts at: 0 28 202 493 664 709 823 1548 2036
Size: 28 174 291 171 45 114 725 488
Cuts at: 2036 2289
Size: 253
Fragments arranged by size:
725 488 291 253 174 171 114 45
28
StuI AGG'CCT
Cuts at: 0 637 2289
Size: 637 1652
StyI C'CwwG_G
Cuts at: 0 124 1484 1689 1928 2177 2289
Size: 124 1360 205 239 249 112
Fragments arranged by size:
1360 249 239 205 124 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 2289
Size: 187 2102
TatI w'GTAC_w
Cuts at: 0 338 509 1949 2139 2289
Size: 338 171 1440 190 150
Fragments arranged by size:
1440 338 190 171 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1260 1292 2289
Size: 1260 32 997
Fragments arranged by size:
1260 997 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 1675 1869 2289
Size: 177 1498 194 420
Fragments arranged by size:
1498 420 194 177
XmnI GAAnn'nnTTC
Cuts at: 0 1516 1840 2289
Size: 1516 324 449
Fragments arranged by size:
1516 449 324
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbsI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII MfeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII ScaI SexAI
SfcI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AscI AseI AsiSI BaeI BbeI BbvCI BciVI
BclI BglII BmtI BpuEI BsaBI BsaWI BsiEI BspEI
BspHI BsrBI BssHII BstBI BstZ17I ClaI CspCI DraI
DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MmeI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI