Restriction map for pF1KB3348
 (Linear) (Six-base) MAPSORT of: KIBB3348.seq  Check: 9337  from: 1  to: 2109



KIBB3348.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     978    2109 
   Size:        978    1131
 
AatII G_ACGT'C

Cuts at:      0    1418    2109 
   Size:       1418     691
 
AccI GT'mk_AC

Cuts at:      0    1280    2109 
   Size:       1280     829
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     269     445    2109 
   Size:         52     217     176    1664

  Fragments arranged by size: 

                1664     217     176      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2063    2109 
   Size:       2063      46
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1167    1275    1537    1554    1581    1932    2016    2100 
   Size:       1167     108     262      17      27     351      84      84

Cuts at:   2100    2109 
   Size:          9

  Fragments arranged by size: 

                1167     351     262     108      84      84      27      17
                   9
 
ApaI G_GGCC'C

Cuts at:      0      76    1860    2109 
   Size:         76    1784     249

  Fragments arranged by size: 

                1784     249      76
 
ApoI r'AATT_y

Cuts at:      0    1405    1944    2109 
   Size:       1405     539     165

  Fragments arranged by size: 

                1405     539     165
 
AseI AT'TA_AT

Cuts at:      0     502    2109 
   Size:        502    1607
 
AvaI C'yCGr_G

Cuts at:      0    1853    2109 
   Size:       1853     256
 
BamHI G'GATC_C

Cuts at:      0     671    2109 
   Size:        671    1438
 
BanII G_rGCy'C

Cuts at:      0      76     243     332     361     372     763    1211    1860 
   Size:         76     167      89      29      11     391     448     649

Cuts at:   1860    2109 
   Size:        249

  Fragments arranged by size: 

                 649     448     391     249     167      89      76      29
                  11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     617     651    1006    1040    2109 
   Size:        617      34     355      34    1069

  Fragments arranged by size: 

                1069     617     355      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     759    2109 
   Size:        759    1350
 
BglI GCCn_nnn'nGGC

Cuts at:      0     632    2109 
   Size:        632    1477
 
BlpI GC'TnA_GC

Cuts at:      0     331     757     856    1174    1300    2109 
   Size:        331     426      99     318     126     809

  Fragments arranged by size: 

                 809     426     331     318     126      99
 
Bme1580I G_kGCm'C

Cuts at:      0      76    1860    1908    2109 
   Size:         76    1784      48     201

  Fragments arranged by size: 

                1784     201      76      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     765    2109 
   Size:        765    1344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     386     417    2008    2041    2109 
   Size:        139     247      31    1591      33      68

  Fragments arranged by size: 

                1591     247     139      68      33      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1441    2109 
   Size:         80    1361     668

  Fragments arranged by size: 

                1361     668      80
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12    1100    1862    2109 
   Size:         12    1088     762     247

  Fragments arranged by size: 

                1088     762     247      12
 
BsaAI yAC'GTr

Cuts at:      0     262    2109 
   Size:        262    1847
 
BsaBI GATnn'nnATC

Cuts at:      0     532    2109 
   Size:        532    1577
 
BsaHI Gr'CG_yC

Cuts at:      0    1415    2109 
   Size:       1415     694
 
BsaWI w'CCGG_w

Cuts at:      0    1669    2109 
   Size:       1669     440
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     182     212     863     893     910     940    2109 
   Size:        182      30     651      30      17      30    1169

  Fragments arranged by size: 

                1169     651     182      30      30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     442     644     681    1057    2109 
   Size:        442     202      37     376    1052

  Fragments arranged by size: 

                1052     442     376     202      37
 
BseYI C'CCAG_C

Cuts at:      0     135     174     270    2051    2109 
   Size:        135      39      96    1781      58

  Fragments arranged by size: 

                1781     135      96      58      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877    1132    1180    1288    1609    1936    2109 
   Size:        877     255      48     108     321     327     173

  Fragments arranged by size: 

                 877     327     321     255     173     108      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     267     763     812    1211    1457    1531    2024    2109 
   Size:        267     496      49     399     246      74     493      85

  Fragments arranged by size: 

                 496     493     399     267     246      85      74      49
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1422    2109 
   Size:       1422     687
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     243     267     332     361     372     763     812 
   Size:         76     167      24      65      29      11     391      49

Cuts at:    812    1211    1457    1531    1860    1908    2024    2109 
   Size:        399     246      74     329      48     116      85

  Fragments arranged by size: 

                 399     391     329     246     167     116      85      76
                  74      65      49      48      29      24      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     452     978    1136    1631    2109 
   Size:        452     526     158     495     478

  Fragments arranged by size: 

                 526     495     478     452     158
 
BsrBI CCG'CTC

Cuts at:      0     204    2109 
   Size:        204    1905
 
BsrDI GCAATG_nn'

Cuts at:      0    1311    1688    1847    2109 
   Size:       1311     377     159     262

  Fragments arranged by size: 

                1311     377     262     159
 
BsrGI T'GTAC_A

Cuts at:      0     926    2109 
   Size:        926    1183
 
BssHII G'CGCG_C

Cuts at:      0    2001    2109 
   Size:       2001     108
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     993    1194    1932    2109 
   Size:        993     201     738     177

  Fragments arranged by size: 

                 993     738     201     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     113    2109 
   Size:        113    1996
 
BstYI r'GATC_y

Cuts at:      0     671     959    2012    2109 
   Size:        671     288    1053      97

  Fragments arranged by size: 

                1053     671     288      97
 
BstZ17I GTA'TAC

Cuts at:      0    1281    2109 
   Size:       1281     828
 
BtgI C'CryG_G

Cuts at:      0    1873    1950    2109 
   Size:       1873      77     159

  Fragments arranged by size: 

                1873     159      77
 
BtsI GCAGTG_nn'

Cuts at:      0     190     977    1614    1924    2109 
   Size:        190     787     637     310     185

  Fragments arranged by size: 

                 787     637     310     190     185
 
DraI TTT'AAA

Cuts at:      0     797    2109 
   Size:        797    1312
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1876    2109 
   Size:       1876     233
 
EaeI y'GGCC_r

Cuts at:      0     212     974    1844    2109 
   Size:        212     762     870     265

  Fragments arranged by size: 

                 870     762     265     212
 
EarI CTCTTCn'nnn_

Cuts at:      0     598    1519    2109 
   Size:        598     921     590

  Fragments arranged by size: 

                 921     598     590
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1116    2109 
   Size:       1116     993
 
EcoICRI GAG'CTC

Cuts at:      0     761    1209    2109 
   Size:        761     448     900

  Fragments arranged by size: 

                 900     761     448
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     139     269     386     417     445    2008    2041 
   Size:         52      87     130     117      31      28    1563      33

Cuts at:   2041    2109 
   Size:         68

  Fragments arranged by size: 

                1563     130     117      87      68      52      33      31
                  28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1891    2109 
   Size:       1891     218
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72      73     125     254     708    1071    1507    1857 
   Size:         72       1      52     129     454     363     436     350

Cuts at:   1857    1878    1929    2070    2109 
   Size:         21      51     141      39

  Fragments arranged by size: 

                 454     436     363     350     141     129      72      52
                  51      39      21       1
 
EcoRI G'AATT_C

Cuts at:      0    1944    2109 
   Size:       1944     165
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1090    2109 
   Size:       1090    1019
 
HaeII r_GCGC'y

Cuts at:      0    1501    2109 
   Size:       1501     608
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262     863     895    2057    2089    2109 
   Size:        230      32     601      32    1162      32      20

  Fragments arranged by size: 

                1162     601     230      32      32      32      20
 
HindIII A'AGCT_T

Cuts at:      0    2044    2109 
   Size:       2044      65
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    2109 
   Size:        987    1122
 
MscI TGG'CCA

Cuts at:      0     214     976    1846    2109 
   Size:        214     762     870     263

  Fragments arranged by size: 

                 870     762     263     214
 
MslI CAynn'nnrTG

Cuts at:      0     775    1754    2109 
   Size:        775     979     355

  Fragments arranged by size: 

                 979     775     355
 
MspA1I CmG'CkG

Cuts at:      0     335     916    1092    1818    1875    2097    2109 
   Size:        335     581     176     726      57     222      12

  Fragments arranged by size: 

                 726     581     335     222     176      57      12
 
NspI r_CATG'y

Cuts at:      0     900    2109 
   Size:        900    1209
 
PasI CC'CwG_GG

Cuts at:      0    1215    2109 
   Size:       1215     894
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1773    2016    2109 
   Size:       1773     243      93

  Fragments arranged by size: 

                1773     243      93
 
PfoI T'CCnGG_A

Cuts at:      0     363     674     722     962    1766    1886    2024    2057 
   Size:        363     311      48     240     804     120     138      33

Cuts at:   2057    2109 
   Size:         52

  Fragments arranged by size: 

                 804     363     311     240     138     120      52      48
                  33
 
PmlI CAC'GTG

Cuts at:      0     262    2109 
   Size:        262    1847
 
PpuMI rG'GwC_Cy

Cuts at:      0     125     708    1507    1878    2070    2109 
   Size:        125     583     799     371     192      39

  Fragments arranged by size: 

                 799     583     371     192     125      39
 
PspOMI G'GGCC_C

Cuts at:      0      72    1856    2109 
   Size:         72    1784     253

  Fragments arranged by size: 

                1784     253      72
 
PstI C_TGCA'G

Cuts at:      0     621    1019    1190    1320    1394    1550    1706    1937 
   Size:        621     398     171     130      74     156     156     231

Cuts at:   1937    1991    2042    2109 
   Size:         54      51      67

  Fragments arranged by size: 

                 621     398     231     171     156     156     130      74
                  67      54      51
 
PvuII CAG'CTG

Cuts at:      0     335    1092    1818    2109 
   Size:        335     757     726     291

  Fragments arranged by size: 

                 757     726     335     291
 
SacI G_AGCT'C

Cuts at:      0     763    1211    2109 
   Size:        763     448     898

  Fragments arranged by size: 

                 898     763     448
 
SacII CC_GC'GG

Cuts at:      0    1876    2109 
   Size:       1876     233
 
SanDI GG'GwC_CC

Cuts at:      0     125    1507    2109 
   Size:        125    1382     602

  Fragments arranged by size: 

                1382     602     125
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1519    2109 
   Size:       1519     590
 
SbfI CC_TGCA'GG

Cuts at:      0    1937    2109 
   Size:       1937     172
 
SexAI A'CCwGG_T

Cuts at:      0     389    2109 
   Size:        389    1720
 
SfcI C'TryA_G

Cuts at:      0     617    1015    1186    1316    1390    1546    1702    1933 
   Size:        617     398     171     130      74     156     156     231

Cuts at:   1933    1987    2038    2109 
   Size:         54      51      71

  Fragments arranged by size: 

                 617     398     231     171     156     156     130      74
                  71      54      51
 
SmaI CCC'GGG

Cuts at:      0    1855    2109 
   Size:       1855     254
 
SmlI C'TyrA_G

Cuts at:      0      95    1456    2109 
   Size:         95    1361     653

  Fragments arranged by size: 

                1361     653      95
 
SpeI A'CTAG_T

Cuts at:      0    1900    2109 
   Size:       1900     209
 
SrfI GCCC'GGGC

Cuts at:      0    1855    2109 
   Size:       1855     254
 
StuI AGG'CCT

Cuts at:      0    1449    2109 
   Size:       1449     660
 
StyI C'CwwG_G

Cuts at:      0     292    2109 
   Size:        292    1817
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     481    1228    1358    2109 
   Size:        481     747     130     751

  Fragments arranged by size: 

                 751     747     481     130
 
TatI w'GTAC_w

Cuts at:      0     926    2109 
   Size:        926    1183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214    1935    1967    2109 
   Size:        182      32    1721      32     142

  Fragments arranged by size: 

                1721     182     142      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1853    2109 
   Size:       1853     256
 
XmnI GAAnn'nnTTC

Cuts at:      0     950    1327    2109 
   Size:        950     377     782

  Fragments arranged by size: 

                 950     782     377
 
ZraI GAC'GTC

Cuts at:      0    1416    2109 
   Size:       1416     693
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AlfI    AlwNI     ApaI     ApoI
     AseI     AvaI    BamHI    BanII     BcgI    BciVI     BglI     BlpI
 Bme1580I     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstXI    BstYI
  BstZ17I     BtgI     BtsI     DraI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
  HindIII     MmeI     MscI     MslI   MspA1I     NspI     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
    SanDI     SapI     SbfI    SexAI     SfcI     SmaI     SmlI     SpeI
     SrfI     StuI     StyI    TaqII     TatI     TstI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
   Bpu10I    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrFI    BssSI
    BstBI   BstEII   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SphI     SspI     SwaI  Tth111I
     XbaI     XcmI     XhoI
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