Restriction map for pF1KB3329
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbvCI       BcgI        
BfrI        BglI        BglII       BlnI        BsaBI       
BsiWI       BspDI       BspHI       BspLU11I    BstBI       
Bsu36I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT14I     EcoT22I     FseI        FspI        HindIII     
KpnI        MamI        MfeI        MunI        NcoI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        StyI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         2148  GTATAC               GT^[AC][GT]AC
AccIII        615  TCCGGA               T^CCGGA
ApaI         2283  GGGCCC               GGGCC^C
AvaI         1369  CCCGAG               C^[CT]CG[GA]G
BalI         2512  TGGCCA               TGG^CCA
BbrPI        1762  CACGTG               CAC^GTG
BlpI         1084  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1084  GCTCAGC              GC^T[ACGT]AGC
BsaI         2758  GAGACC               GGTCTC(1/5)
BseAI         615  TCCGGA               T^CCGGA
BsoBI        1369  CCCGAG               C^[CT]CG[GA]G
BspEI         615  TCCGGA               T^CCGGA
BsrDI        1101  CATTGC               GCAATG(2/0)
BssSI        1570  CACGAG               CACGAG(-5/-1)
Bst1107I     2148  GTATAC               GTA^TAC
BstAPI       1783  GCAGTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1149  GGTGACC              G^GT[ACGT]ACC
BstPI        1149  GGTGACC              G^GT[ACGT]ACC
BstYI        2671  GGATCT               [GA]^GATC[CT]
BstZ17I      2148  GTATAC               GTA^TAC
CelII        1084  GCTCAGC              GC^T[ACGT]AGC
DraIII       1762  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1149  GGTGACC              G^GT[ACGT]ACC
HpaI         1726  GTTAAC               GTT^AAC
KspI         1741  CCGCGG               CCGC^GG
MflI         2671  GGATCT               [GA]^GATC[CT]
MluNI        2512  TGGCCA               TGG^CCA
MroI          615  TCCGGA               T^CCGGA
MscI         2512  TGGCCA               TGG^CCA
NdeI         1820  CATATG               CA^TATG
NspI         2441  GCATGC               [GA]CATG^[CT]
PmaCI        1762  CACGTG               CAC^GTG
PmlI         1762  CACGTG               CAC^GTG
PvuII        2592  CAGCTG               CAG^CTG
SacII        1741  CCGCGG               CCGC^GG
ScaI         1941  AGTACT               AGT^ACT
SgrAI         389  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         2441  GCATGC               GCATG^C
StuI         2027  AGGCCT               AGG^CCT
XcmI         1313  CCAGGGTATCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        2671  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Alw44I       1525  GTGCAC               G^TGCAC
             2455  GTGCAC              
ApaLI        1525  GTGCAC               G^TGCAC
             2455  GTGCAC              
BanII        2058  GAGCCC               G[GA]GC[CT]^C
             2283  GGGCCC              
BbeI         2019  GGCGCC               GGCGC^C
             2050  GGCGCC              
BbsI          863  GAAGAC               GAAGAC(2/6)
             2321  GTCTTC              
BciVI        1318  GTATCC               GTATCC(6/5)
             2521  GTATCC              
BpuAI         863  GAAGAC               GAAGAC(2/6)
             2321  GTCTTC              
BsaAI        1516  TACGTG               [CT]AC^GT[GA]
             1762  CACGTG              
BsaWI         615  TCCGGA               [AT]^CCGG[AT]
             2632  TCCGGT              
BsgI          178  GTGCAG               GTGCAG(16/14)
              303  GTGCAG              
BsmBI         102  CGTCTC               CGTCTC(1/5)
              806  GAGACG              
BsmI          492  GAATGC               GAATGC(1/-1)
             2474  GCATTC              
Bsp1407I      997  TGTACA               T^GTACA
             1874  TGTACA              
BsrGI         997  TGTACA               T^GTACA
             1874  TGTACA              
DraI          585  TTTAAA               TTT^AAA
             2602  TTTAAA              
EarI         2031  CTCTTC               CTCTTC(1/4)
              487  GAAGAG              
EcoRI          64  GAATTC               G^AATTC
              258  GAATTC              
KasI         2019  GGCGCC               G^GCGCC
             2050  GGCGCC              
Ksp632I      2031  CTCTTC               CTCTTC(1/4)
              487  GAAGAG              
MluI         1808  ACGCGT               A^CGCGT
             1897  ACGCGT              
NarI         2019  GGCGCC               GG^CGCC
             2050  GGCGCC              
PpuMI         149  AGGACCT              [GA]G^G[AT]CC[CT]
              215  GGGACCT             
SfoI         2019  GGCGCC               GGC^GCC
             2050  GGCGCC              
SspBI         997  TGTACA               T^GTACA
             1874  TGTACA              
TfiI          430  GAATC                G^A[AT]TC
              541  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AspHI       AvaII       BanI        BbvI        BclI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
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