Restriction map for pF1KB3329
 (Linear) (Six-base) MAPSORT of: KIBB3329.seq  Check: 1507  from: 1  to: 2847



KIBB3329.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1641    2847 
   Size:       1641    1206
 
AccI GT'mk_AC

Cuts at:      0    2149    2847 
   Size:       2149     698
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1061    2295    2847 
   Size:       1061    1234     552

  Fragments arranged by size: 

                1234    1061     552
 
AflIII A'CryG_T

Cuts at:      0    1247    1517    1808    1897    2087    2847 
   Size:       1247     270     291      89     190     760

  Fragments arranged by size: 

                1247     760     291     270     190      89
 
AleI CACnn'nnGTG

Cuts at:      0     130    2212    2847 
   Size:        130    2082     635

  Fragments arranged by size: 

                2082     635     130
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153     186    2068    2847 
   Size:        153      33    1882     779

  Fragments arranged by size: 

                1882     779     153      33
 
ApaI G_GGCC'C

Cuts at:      0    2287    2847 
   Size:       2287     560
 
ApaLI G'TGCA_C

Cuts at:      0    1525    2455    2847 
   Size:       1525     930     392

  Fragments arranged by size: 

                1525     930     392
 
ApoI r'AATT_y

Cuts at:      0      64     258    2656    2847 
   Size:         64     194    2398     191

  Fragments arranged by size: 

                2398     194     191      64
 
AvaI C'yCGr_G

Cuts at:      0    1369    2847 
   Size:       1369    1478
 
BanI G'GyrC_C

Cuts at:      0      28     124     670    2019    2050    2847 
   Size:         28      96     546    1349      31     797

  Fragments arranged by size: 

                1349     797     546      96      31      28
 
BanII G_rGCy'C

Cuts at:      0    2062    2287    2847 
   Size:       2062     225     560

  Fragments arranged by size: 

                2062     560     225
 
BbeI G_GCGC'C

Cuts at:      0    2023    2054    2847 
   Size:       2023      31     793

  Fragments arranged by size: 

                2023     793      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     870    2314    2847 
   Size:        870    1444     533

  Fragments arranged by size: 

                1444     870     533
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1329    2532    2847 
   Size:       1329    1203     315

  Fragments arranged by size: 

                1329    1203     315
 
BclI T'GATC_A

Cuts at:      0    1109    2096    2502    2847 
   Size:       1109     987     406     345

  Fragments arranged by size: 

                1109     987     406     345
 
BlpI GC'TnA_GC

Cuts at:      0    1085    2847 
   Size:       1085    1762
 
Bme1580I G_kGCm'C

Cuts at:      0      31    1529    1905    2287    2459    2847 
   Size:         31    1498     376     382     172     388

  Fragments arranged by size: 

                1498     388     382     376     172      31
 
BmgBI CAC'GTC

Cuts at:      0    1338    2847 
   Size:       1338    1509
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1063    2312    2847 
   Size:       1063    1249     535

  Fragments arranged by size: 

                1249    1063     535
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1935    2847 
   Size:       1935     912
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337    1600    2549    2847 
   Size:        181      63      93    1263     949     298

  Fragments arranged by size: 

                1263     949     298     181      93      63
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2752    2847 
   Size:       2752      95
 
BsaAI yAC'GTr

Cuts at:      0    1518    1764    2847 
   Size:       1518     246    1083

  Fragments arranged by size: 

                1518    1083     246
 
BsaHI Gr'CG_yC

Cuts at:      0     199    1165    2020    2051    2847 
   Size:        199     966     855      31     796

  Fragments arranged by size: 

                 966     855     796     199      31
 
BsaWI w'CCGG_w

Cuts at:      0     615    2632    2847 
   Size:        615    2017     215

  Fragments arranged by size: 

                2017     615     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1474    1504    1709    1739    2356    2386    2410    2440 
   Size:       1474      30     205      30     617      30      24      30

Cuts at:   2440    2847 
   Size:        407

  Fragments arranged by size: 

                1474     617     407     205      30      30      30      30
                  24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      49     124     292    1588    2293    2359    2479 
   Size:         46       3      75     168    1296     705      66     120

Cuts at:   2479    2770    2847 
   Size:        291      77

  Fragments arranged by size: 

                1296     705     291     168     120      77      75      66
                  46       3
 
BseYI C'CCAG_C

Cuts at:      0    1090    2847 
   Size:       1090    1757
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     324    2847 
   Size:        199     125    2523

  Fragments arranged by size: 

                2523     199     125
 
BsiEI CG_ry'CG

Cuts at:      0     273    1500    1747    2847 
   Size:        273    1227     247    1100

  Fragments arranged by size: 

                1227    1100     273     247
 
BsiHKAI G_wGCw'C

Cuts at:      0     785    1529    2221    2275    2459    2847 
   Size:        785     744     692      54     184     388

  Fragments arranged by size: 

                 785     744     692     388     184      54
 
BsmI GAATG_Cn'

Cuts at:      0     498    2474    2847 
   Size:        498    1976     373

  Fragments arranged by size: 

                1976     498     373
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     108     800    2847 
   Size:        108     692    2047

  Fragments arranged by size: 

                2047     692     108
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     785    1529    1905    2062    2221    2275    2287 
   Size:         31     754     744     376     157     159      54      12

Cuts at:   2287    2459    2847 
   Size:        172     388

  Fragments arranged by size: 

                 754     744     388     376     172     159     157      54
                  31      12
 
BspEI T'CCGG_A

Cuts at:      0     615    2847 
   Size:        615    2232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     333     979    1162    1641    2847 
   Size:        333     646     183     479    1206

  Fragments arranged by size: 

                1206     646     479     333     183
 
BsrBI CCG'CTC

Cuts at:      0      83    1485    1538    2012    2847 
   Size:         83    1402      53     474     835

  Fragments arranged by size: 

                1402     835     474      83      53
 
BsrDI GCAATG_nn'

Cuts at:      0    1100    2847 
   Size:       1100    1747
 
BsrFI r'CCGG_y

Cuts at:      0     390    1683    1833    1965    2847 
   Size:        390    1293     150     132     882

  Fragments arranged by size: 

                1293     882     390     150     132
 
BsrGI T'GTAC_A

Cuts at:      0     997    1874    2847 
   Size:        997     877     973

  Fragments arranged by size: 

                 997     973     877
 
BssHII G'CGCG_C

Cuts at:      0    1502    1604    1606    1779    2192    2847 
   Size:       1502     102       2     173     413     655

  Fragments arranged by size: 

                1502     655     413     173     102       2
 
BssSI C'ACGA_G

Cuts at:      0    1570    2847 
   Size:       1570    1277
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1789    2847 
   Size:       1789    1058
 
BstEII G'GTnAC_C

Cuts at:      0    1149    2847 
   Size:       1149    1698
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1992    2112    2127    2847 
   Size:       1992     120      15     720

  Fragments arranged by size: 

                1992     720     120      15
 
BstYI r'GATC_y

Cuts at:      0    2671    2847 
   Size:       2671     176
 
BstZ17I GTA'TAC

Cuts at:      0    2150    2847 
   Size:       2150     697
 
BtgI C'CryG_G

Cuts at:      0    1307    1322    1741    1979    2847 
   Size:       1307      15     419     238     868

  Fragments arranged by size: 

                1307     868     419     238      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     126    1625    2085    2847 
   Size:        126    1499     460     762

  Fragments arranged by size: 

                1499     762     460     126
 
BtsI GCAGTG_nn'

Cuts at:      0    1687    1790    2515    2847 
   Size:       1687     103     725     332

  Fragments arranged by size: 

                1687     725     332     103
 
DraI TTT'AAA

Cuts at:      0     587    2604    2847 
   Size:        587    2017     243

  Fragments arranged by size: 

                2017     587     243
 
DraIII CAC_nnn'GTG

Cuts at:      0    1767    2847 
   Size:       1767    1080
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     814    1059    1135    2847 
   Size:        814     245      76    1712

  Fragments arranged by size: 

                1712     814     245      76
 
EaeI y'GGCC_r

Cuts at:      0      84    1310    1331    1976    1982    2512    2847 
   Size:         84    1226      21     645       6     530     335

  Fragments arranged by size: 

                1226     645     530     335      84      21       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     482    2037    2847 
   Size:        482    1555     810

  Fragments arranged by size: 

                1555     810     482
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     184    2847 
   Size:        184    2663
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337    1061    1600    2295    2549    2847 
   Size:        181      63      93     724     539     695     254     298

  Fragments arranged by size: 

                 724     695     539     298     254     181      93      63
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223    1909    2464    2847 
   Size:        223    1686     555     383

  Fragments arranged by size: 

                1686     555     383     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     216    2283    2497    2847 
   Size:        150      66    2067     214     350

  Fragments arranged by size: 

                2067     350     214     150      66
 
EcoRI G'AATT_C

Cuts at:      0      64     258    2847 
   Size:         64     194    2589

  Fragments arranged by size: 

                2589     194      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     793    2847 
   Size:        793    2054
 
HaeII r_GCGC'y

Cuts at:      0     186    1232    1972    2023    2054    2158    2847 
   Size:        186    1046     740      51      31     104     689

  Fragments arranged by size: 

                1046     740     689     186     104      51      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61    1114    1146    1780    1812    2356    2388 
   Size:         29      32    1053      32     634      32     544      32

Cuts at:   2388    2847 
   Size:        459

  Fragments arranged by size: 

                1053     634     544     459      32      32      32      32
                  29
 
HincII GTy'rAC

Cuts at:      0     705    1140    1728    2085    2583    2847 
   Size:        705     435     588     357     498     264

  Fragments arranged by size: 

                 705     588     498     435     357     264
 
HpaI GTT'AAC

Cuts at:      0    1728    2847 
   Size:       1728    1119
 
KasI G'GCGC_C

Cuts at:      0    2019    2050    2847 
   Size:       2019      31     797

  Fragments arranged by size: 

                2019     797      31
 
MluI A'CGCG_T

Cuts at:      0    1808    1897    2847 
   Size:       1808      89     950

  Fragments arranged by size: 

                1808     950      89
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1544    2847 
   Size:       1544    1303
 
MscI TGG'CCA

Cuts at:      0    2514    2847 
   Size:       2514     333
 
MslI CAynn'nnrTG

Cuts at:      0     130    2110    2212    2847 
   Size:        130    1980     102     635

  Fragments arranged by size: 

                1980     635     130     102
 
MspA1I CmG'CkG

Cuts at:      0     339    1743    2594    2847 
   Size:        339    1404     851     253

  Fragments arranged by size: 

                1404     851     339     253
 
NaeI GCC'GGC

Cuts at:      0    1685    1835    1967    2847 
   Size:       1685     150     132     880

  Fragments arranged by size: 

                1685     880     150     132
 
NarI GG'CG_CC

Cuts at:      0    2020    2051    2847 
   Size:       2020      31     796

  Fragments arranged by size: 

                2020     796      31
 
NdeI CA'TA_TG

Cuts at:      0    1821    2847 
   Size:       1821    1026
 
NgoMIV G'CCGG_C

Cuts at:      0    1683    1833    1965    2847 
   Size:       1683     150     132     882

  Fragments arranged by size: 

                1683     882     150     132
 
NspI r_CATG'y

Cuts at:      0    2445    2847 
   Size:       2445     402
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     553     696    1767    2847 
   Size:        553     143    1071    1080

  Fragments arranged by size: 

                1080    1071     553     143
 
PfoI T'CCnGG_A

Cuts at:      0    2035    2490    2847 
   Size:       2035     455     357

  Fragments arranged by size: 

                2035     455     357
 
PmlI CAC'GTG

Cuts at:      0    1764    2847 
   Size:       1764    1083
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     216    2847 
   Size:        150      66    2631

  Fragments arranged by size: 

                2631     150      66
 
PsiI TTA'TAA

Cuts at:      0     944    2847 
   Size:        944    1903
 
PspOMI G'GGCC_C

Cuts at:      0    2283    2847 
   Size:       2283     564
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    2847 
   Size:       1366      32    1449

  Fragments arranged by size: 

                1449    1366      32
 
PstI C_TGCA'G

Cuts at:      0     239     344    1559    1652    2847 
   Size:        239     105    1215      93    1195

  Fragments arranged by size: 

                1215    1195     239     105      93
 
PvuII CAG'CTG

Cuts at:      0    2594    2847 
   Size:       2594     253
 
SacII CC_GC'GG

Cuts at:      0    1744    2847 
   Size:       1744    1103
 
ScaI AGT'ACT

Cuts at:      0    1943    2847 
   Size:       1943     904
 
SfcI C'TryA_G

Cuts at:      0      40     235     340     507     641     962    1555    1588 
   Size:         40     195     105     167     134     321     593      33

Cuts at:   1588    1648    2847 
   Size:         60    1199

  Fragments arranged by size: 

                1199     593     321     195     167     134     105      60
                  40      33
 
SfoI GGC'GCC

Cuts at:      0    2021    2052    2847 
   Size:       2021      31     795

  Fragments arranged by size: 

                2021     795      31
 
SgrAI Cr'CCGG_yG

Cuts at:      0     390    2847 
   Size:        390    2457
 
SphI G_CATG'C

Cuts at:      0    2445    2847 
   Size:       2445     402
 
StuI AGG'CCT

Cuts at:      0    2029    2847 
   Size:       2029     818
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     818     996    1741    2847 
   Size:        818     178     745    1106

  Fragments arranged by size: 

                1106     818     745     178
 
TatI w'GTAC_w

Cuts at:      0     954     997    1373    1874    1941    2847 
   Size:        954      43     376     501      67     906

  Fragments arranged by size: 

                 954     906     501     376      67      43
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1320    2847 
   Size:       1320    1527
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BciVI     BclI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII     HpaI     KasI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NspI    PflMI     PfoI     PmlI
    PpuMI     PsiI   PspOMI     PsrI     PstI    PvuII    SacII     ScaI
     SfcI     SfoI    SgrAI     SphI     StuI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BfrBI     BglI    BglII     BmtI   Bpu10I    BpuEI    BsaBI
    BsiWI    BspHI    BstBI   Bsu36I     ClaI    CspCI     EagI  EcoICRI
    EcoRV     FseI     FspI    FspAI  HindIII     KpnI     MfeI     NcoI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PpiI    PshAI    PspXI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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