Restriction map for pF1KB3304
 (Linear) (Six-base) MAPSORT of: KIBB3304.seq  Check: 766  from: 1  to: 1143



KIBB3304.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0     311    1143 
   Size:        311     832
 
AfeI AGC'GCT

Cuts at:      0      86     303    1143 
   Size:         86     217     840

  Fragments arranged by size: 

                 840     217      86
 
AflIII A'CryG_T

Cuts at:      0    1068    1143 
   Size:       1068      75
 
AlwNI CAG_nnn'CTG

Cuts at:      0     201     640     901    1143 
   Size:        201     439     261     242

  Fragments arranged by size: 

                 439     261     242     201
 
ApaLI G'TGCA_C

Cuts at:      0     114    1143 
   Size:        114    1029
 
ApoI r'AATT_y

Cuts at:      0     742    1143 
   Size:        742     401
 
BanII G_rGCy'C

Cuts at:      0      59    1143 
   Size:         59    1084
 
BbvCI CC'TCA_GC

Cuts at:      0     305    1143 
   Size:        305     838
 
BclI T'GATC_A

Cuts at:      0     531    1143 
   Size:        531     612
 
BfrBI ATG'CAT

Cuts at:      0     808    1143 
   Size:        808     335
 
Bme1580I G_kGCm'C

Cuts at:      0     118    1143 
   Size:        118    1025
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     259    1143 
   Size:        259     884
 
Bpu10I CC'TnA_GC

Cuts at:      0     305    1143 
   Size:        305     838
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213    1143 
   Size:        213     930
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     126     471    1143 
   Size:        126     345     672

  Fragments arranged by size: 

                 672     345     126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     473     503    1143 
   Size:        473      30     640

  Fragments arranged by size: 

                 640     473      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     193     239     242    1143 
   Size:         43     150      46       3     901

  Fragments arranged by size: 

                 901     150      46      43       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     841     871    1143 
   Size:        127     714      30     272

  Fragments arranged by size: 

                 714     272     127      30
 
BsiEI CG_ry'CG

Cuts at:      0     681    1143 
   Size:        681     462
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     118    1143 
   Size:         59      59    1025

  Fragments arranged by size: 

                1025      59      59
 
BsmI GAATG_Cn'

Cuts at:      0     142    1143 
   Size:        142    1001
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     535    1143 
   Size:        535     608
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     118    1143 
   Size:         59      59    1025

  Fragments arranged by size: 

                1025      59      59
 
BssSI C'ACGA_G

Cuts at:      0    1001    1022    1143 
   Size:       1001      21     121

  Fragments arranged by size: 

                1001     121      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267    1143 
   Size:        267     876
 
Bsu36I CC'TnA_GG

Cuts at:      0     148    1143 
   Size:        148     995
 
BtgI C'CryG_G

Cuts at:      0     137    1143 
   Size:        137    1006
 
BtsI GCAGTG_nn'

Cuts at:      0     116    1143 
   Size:        116    1027
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     999    1034    1143 
   Size:        999      35     109

  Fragments arranged by size: 

                 999     109      35
 
EaeI y'GGCC_r

Cuts at:      0     374    1143 
   Size:        374     769
 
EarI CTCTTCn'nnn_

Cuts at:      0     923    1143 
   Size:        923     220
 
EcoICRI GAG'CTC

Cuts at:      0      57    1143 
   Size:         57    1086
 
EcoO109I rG'GnC_Cy

Cuts at:      0     955    1143 
   Size:        955     188
 
EcoRI G'AATT_C

Cuts at:      0     742    1143 
   Size:        742     401
 
HaeII r_GCGC'y

Cuts at:      0      88     305    1143 
   Size:         88     217     838

  Fragments arranged by size: 

                 838     217      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     183     215     234     266     281     313     316     348 
   Size:        183      32      19      32      15      32       3      32

Cuts at:    348     981    1013    1143 
   Size:        633      32     130

  Fragments arranged by size: 

                 633     183     130      32      32      32      32      32
                  19      15       3
 
HincII GTy'rAC

Cuts at:      0     558    1143 
   Size:        558     585
 
HpaI GTT'AAC

Cuts at:      0     558    1143 
   Size:        558     585
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     149    1143 
   Size:        149     994
 
MslI CAynn'nnrTG

Cuts at:      0     422    1143 
   Size:        422     721
 
MspA1I CmG'CkG

Cuts at:      0     898    1143 
   Size:        898     245
 
NcoI C'CATG_G

Cuts at:      0     137    1143 
   Size:        137    1006
 
NsiI A_TGCA'T

Cuts at:      0     810    1143 
   Size:        810     333
 
PshAI GACnn'nnGTC

Cuts at:      0     547    1143 
   Size:        547     596
 
PstI C_TGCA'G

Cuts at:      0     206    1143 
   Size:        206     937
 
PvuII CAG'CTG

Cuts at:      0     898    1143 
   Size:        898     245
 
SacI G_AGCT'C

Cuts at:      0      59    1143 
   Size:         59    1084
 
SfcI C'TryA_G

Cuts at:      0     202     690    1143 
   Size:        202     488     453

  Fragments arranged by size: 

                 488     453     202
 
SmlI C'TyrA_G

Cuts at:      0     228    1143 
   Size:        228     915
 
StyI C'CwwG_G

Cuts at:      0     137    1143 
   Size:        137    1006
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     273     453     695     905    1067    1143 
   Size:        273     180     242     210     162      76

  Fragments arranged by size: 

                 273     242     210     180     162      76
 
TatI w'GTAC_w

Cuts at:      0    1115    1143 
   Size:       1115      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     473     505    1143 
   Size:        473      32     638

  Fragments arranged by size: 

                 638     473      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1070    1143 
   Size:       1070      73
 

 Enzymes that do cut:

     AccI     AfeI   AflIII    AlwNI    ApaLI     ApoI    BanII    BbvCI
     BclI    BfrBI Bme1580I     BmrI   Bpu10I    BpuEI     BsaI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BssSI
    BstXI   Bsu36I     BtgI     BtsI    CspCI     EaeI     EarI  EcoICRI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII     HpaI     MmeI     MslI
   MspA1I     NcoI     NsiI    PshAI     PstI    PvuII     SacI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
     EciI  Eco57MI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com