Restriction map for pF1KB3253
 (Linear) (Six-base) MAPSORT of: KIBB3253.seq  Check: 1825  from: 1  to: 1863



KIBB3253.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     826    1252    1863 
   Size:        207     619     426     611

  Fragments arranged by size: 

                 619     611     426     207
 
AflIII A'CryG_T

Cuts at:      0    1490    1863 
   Size:       1490     373
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1503    1535    1863 
   Size:       1503      32     328

  Fragments arranged by size: 

                1503     328      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1722    1819    1863 
   Size:       1722      97      44

  Fragments arranged by size: 

                1722      97      44
 
ApoI r'AATT_y

Cuts at:      0    1132    1246    1863 
   Size:       1132     114     617

  Fragments arranged by size: 

                1132     617     114
 
AvaI C'yCGr_G

Cuts at:      0     912    1863 
   Size:        912     951
 
AvrII C'CTAG_G

Cuts at:      0    1522    1863 
   Size:       1522     341
 
BanI G'GyrC_C

Cuts at:      0     638    1863 
   Size:        638    1225
 
BanII G_rGCy'C

Cuts at:      0     913    1343    1863 
   Size:        913     430     520

  Fragments arranged by size: 

                 913     520     430
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     779     948    1363    1394    1863 
   Size:        779     169     415      31     469

  Fragments arranged by size: 

                 779     469     415     169      31
 
BbvCI CC'TCA_GC

Cuts at:      0     392    1863 
   Size:        392    1471
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     705    1863 
   Size:        705    1158
 
BfrBI ATG'CAT

Cuts at:      0     325    1863 
   Size:        325    1538
 
BglII A'GATC_T

Cuts at:      0     315    1863 
   Size:        315    1548
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     553    1863 
   Size:        553    1310
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1357    1863 
   Size:       1357     506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664    1863 
   Size:        664    1199
 
Bpu10I CC'TnA_GC

Cuts at:      0     392    1863 
   Size:        392    1471
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1327    1863 
   Size:       1327     536
 
BsaWI w'CCGG_w

Cuts at:      0      11     539     898    1863 
   Size:         11     528     359     965

  Fragments arranged by size: 

                 965     528     359      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     913    1343    1863 
   Size:        913     430     520

  Fragments arranged by size: 

                 913     520     430
 
BsmI GAATG_Cn'

Cuts at:      0     735    1863 
   Size:        735    1128
 
Bsp1286I G_dGCh'C

Cuts at:      0     913    1343    1863 
   Size:        913     430     520

  Fragments arranged by size: 

                 913     520     430
 
BspEI T'CCGG_A

Cuts at:      0      11     539    1863 
   Size:         11     528    1324

  Fragments arranged by size: 

                1324     528      11
 
BspHI T'CATG_A

Cuts at:      0     827    1863 
   Size:        827    1036
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1827    1863 
   Size:       1827      36
 
BsrDI GCAATG_nn'

Cuts at:      0     740    1863 
   Size:        740    1123
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1722    1863 
   Size:       1722     141
 
BstBI TT'CG_AA

Cuts at:      0    1746    1863 
   Size:       1746     117
 
BstEII G'GTnAC_C

Cuts at:      0    1162    1863 
   Size:       1162     701
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     642    1159    1863 
   Size:        642     517     704

  Fragments arranged by size: 

                 704     642     517
 
BstYI r'GATC_y

Cuts at:      0     315    1115    1271    1863 
   Size:        315     800     156     592

  Fragments arranged by size: 

                 800     592     315     156
 
BtsI GCAGTG_nn'

Cuts at:      0    1513    1863 
   Size:       1513     350
 
DraI TTT'AAA

Cuts at:      0    1710    1863 
   Size:       1710     153
 
EaeI y'GGCC_r

Cuts at:      0     737     962    1149    1372    1863 
   Size:        737     225     187     223     491

  Fragments arranged by size: 

                 737     491     225     223     187
 
EarI CTCTTCn'nnn_

Cuts at:      0    1331    1748    1793    1863 
   Size:       1331     417      45      70

  Fragments arranged by size: 

                1331     417      70      45
 
EcoICRI GAG'CTC

Cuts at:      0     911    1341    1863 
   Size:        911     430     522

  Fragments arranged by size: 

                 911     522     430
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     664     826    1252    1863 
   Size:        207     457     162     426     611

  Fragments arranged by size: 

                 611     457     426     207     162
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1071    1648    1863 
   Size:       1071     577     215

  Fragments arranged by size: 

                1071     577     215
 
EcoRI G'AATT_C

Cuts at:      0    1246    1863 
   Size:       1246     617
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1279    1419    1863 
   Size:       1279     140     444

  Fragments arranged by size: 

                1279     444     140
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     880     912    1102    1134    1585    1617    1863 
   Size:        880      32     190      32     451      32     246

  Fragments arranged by size: 

                 880     451     246     190      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     786    1136    1863 
   Size:        786     350     727

  Fragments arranged by size: 

                 786     727     350
 
MscI TGG'CCA

Cuts at:      0     739     964    1151    1374    1863 
   Size:        739     225     187     223     489

  Fragments arranged by size: 

                 739     489     225     223     187
 
MspA1I CmG'CkG

Cuts at:      0     751    1863 
   Size:        751    1112
 
NsiI A_TGCA'T

Cuts at:      0     327    1863 
   Size:        327    1536
 
NspI r_CATG'y

Cuts at:      0    1494    1863 
   Size:       1494     369
 
PasI CC'CwG_GG

Cuts at:      0    1044    1863 
   Size:       1044     819
 
PciI A'CATG_T

Cuts at:      0    1490    1863 
   Size:       1490     373
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     622    1863 
   Size:        622    1241
 
PshAI GACnn'nnGTC

Cuts at:      0      22    1863 
   Size:         22    1841
 
PsiI TTA'TAA

Cuts at:      0     335    1863 
   Size:        335    1528
 
PstI C_TGCA'G

Cuts at:      0     954    1863 
   Size:        954     909
 
PvuII CAG'CTG

Cuts at:      0     751    1863 
   Size:        751    1112
 
SacI G_AGCT'C

Cuts at:      0     913    1343    1863 
   Size:        913     430     520

  Fragments arranged by size: 

                 913     520     430
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1331    1748    1863 
   Size:       1331     417     115

  Fragments arranged by size: 

                1331     417     115
 
ScaI AGT'ACT

Cuts at:      0    1402    1863 
   Size:       1402     461
 
SfcI C'TryA_G

Cuts at:      0     950    1071    1416    1751    1863 
   Size:        950     121     345     335     112

  Fragments arranged by size: 

                 950     345     335     121     112
 
SmlI C'TyrA_G

Cuts at:      0    1342    1863 
   Size:       1342     521
 
SspI AAT'ATT

Cuts at:      0     789     819    1863 
   Size:        789      30    1044

  Fragments arranged by size: 

                1044     789      30
 
StyI C'CwwG_G

Cuts at:      0      20    1311    1522    1649    1863 
   Size:         20    1291     211     127     214

  Fragments arranged by size: 

                1291     214     211     127      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      62    1237    1863 
   Size:         62    1175     626

  Fragments arranged by size: 

                1175     626      62
 
TatI w'GTAC_w

Cuts at:      0    1400    1863 
   Size:       1400     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1195    1227    1863 
   Size:       1195      32     636

  Fragments arranged by size: 

                1195     636      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     425    1863 
   Size:        425    1438
 
XmnI GAAnn'nnTTC

Cuts at:      0     818    1229    1259    1863 
   Size:        818     411      30     604

  Fragments arranged by size: 

                 818     604     411      30
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     ApoI     AvaI    AvrII     BanI
    BanII     BbsI    BbvCI    BciVI    BfrBI    BglII     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaWI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI
     FalI    Hin4I     MmeI     MscI   MspA1I     NsiI     NspI     PasI
     PciI    PflMI    PshAI     PsiI     PstI    PvuII     SacI     SapI
     ScaI     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BcgI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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