Restriction map for pF1KB3241
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspI        
AvaI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BseAI       BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MluI        
MroI        NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         2857  GTAGAC               GT^[AC][GT]AC
AccII         999  CGCG                 CG^CG
AhdI          410  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        720  GTGCAC               G^TGCAC
ApaLI         720  GTGCAC               G^TGCAC
AspEI         410  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          729  TGGCCA               TGG^CCA
BbeI         1025  GGCGCC               GGCGC^C
BcgI         1946  GCATGGTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1978  GGATAC               GTATCC(6/5)
BclI         1428  TGATCA               T^GATCA
BcnI         2627  CCCGG                CC^[GC]GG
BsaWI         171  ACCGGA               [AT]^CCGG[AT]
BsmBI        2721  GAGACG               CGTCTC(1/5)
BsrBI        1858  CCGCTC               CCGCTC(-3/-3)
BsrDI         799  CATTGC               GCAATG(2/0)
BstAPI       2342  GCAGTGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2831  TTCGAA               TT^CGAA
BstUI         999  CGCG                 CG^CG
DrdI          922  GACAAGCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      410  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2388  GAATTC               G^AATTC
EcoRV        2152  GATATC               GAT^ATC
FbaI         1428  TGATCA               T^GATCA
HaeII        1025  GGCGCC               [GA]GCGC^[CT]
KasI         1025  GGCGCC               G^GCGCC
MfeI          160  CAATTG               C^AATTG
MluNI         729  TGGCCA               TGG^CCA
MscI          729  TGGCCA               TGG^CCA
MunI          160  CAATTG               C^AATTG
MvnI          999  CGCG                 CG^CG
NaeI         1028  GCCGGC               GCC^GGC
NarI         1025  GGCGCC               GG^CGCC
NciI         2627  CCCGG                CC^[GC]GG
NcoI          390  CCATGG               C^CATGG
NdeI         1937  CATATG               CA^TATG
NgoMIV       1028  GCCGGC               G^CCGGC
NheI         1038  GCTAGC               G^CTAGC
NspV         2831  TTCGAA               TT^CGAA
PpuMI        2837  AGGACCC              [GA]G^G[AT]CC[CT]
SapI          463  GAAGAGC              GCTCTTC(1/4)
ScaI         2595  AGTACT               AGT^ACT
SexAI        2517  ACCAGGT              A^CC[AT]GGT
SfoI         1025  GGCGCC               GGC^GCC
SfuI         2831  TTCGAA               TT^CGAA
SmlI         1797  CTTGAG               C^T[CT][GA]AG
SphI         1744  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwNI        1348  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1541  CAGAGTCTG           
Asp700I       847  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1403  GAATACATTC          
AvaII         387  GGTCC                G^G[AT]CC
             2838  GGACC               
BanI          360  GGCACC               G^G[CT][GA]CC
             1025  GGCGCC              
BbsI         1657  GAAGAC               GAAGAC(2/6)
             2056  GAAGAC              
BpmI         2707  CTCCAG               CTGGAG(16/14)
              115  CTGGAG              
BpuAI        1657  GAAGAC               GAAGAC(2/6)
             2056  GAAGAC              
BsaBI        2187  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2782  GATGCCAATC          
BsaI         2514  GAGACC               GGTCTC(1/5)
              516  GGTCTC              
BseRI          66  CTCCTC               GAGGAG(10/8)
             1579  CTCCTC              
BsgI          782  GTGCAG               GTGCAG(16/14)
             1121  GTGCAG              
BsrFI        1028  GCCGGC               [GA]^CCGG[CT]
             2447  ACCGGC              
CfoI         1026  GCGC                 GCG^C
             1944  GCGC                
Cfr10I       1028  GCCGGC               [GA]^CCGG[CT]
             2447  ACCGGC              
DraI          300  TTTAAA               TTT^AAA
             2767  TTTAAA              
DraII        1720  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             2837  AGGACCC             
DsaI          390  CCATGG               C^C[GA][CT]GG
              676  CCGTGG              
EaeI          729  TGGCCA               [CT]^GGCC[GA]
             2635  CGGCCA              
EcoO109I     1720  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             2837  AGGACCC             
EcoT22I       509  ATGCAT               ATGCA^T
             2046  ATGCAT              
FauI          997  CCCGC                CCCGC(4/6)
              927  GCGGG               
HgaI         2623  GACGC                GACGC(5/10)
             1737  GCGTC               
HhaI         1026  GCGC                 GCG^C
             1944  GCGC                
HinP1I       1026  GCGC                 G^CGC
             1944  GCGC                
MaeII         604  ACGT                 A^CGT
              718  ACGT                
MamI         2187  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2782  GATGCCAATC          
NsiI          509  ATGCAT               ATGCA^T
             2046  ATGCAT              
NspI          887  ACATGC               [GA]CATG^[CT]
             1744  GCATGC              
SacI         1903  GAGCTC               GAGCT^C
             2575  GAGCTC              
SspI         1447  AATATT               AAT^ATT
             1681  AATATT              
XmnI          847  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1403  GAATACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspHI       BanII       BbvI        
BfaI        BglII       BmyI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       Hin1I       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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