Restriction map for pF1KB3241
 (Linear) (Six-base) MAPSORT of: KIBB3241.seq  Check: 5186  from: 1  to: 2877



KIBB3241.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0    2858    2877 
   Size:       2858      19
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     838    1303    1595    1676    2877 
   Size:        301     537     465     292      81    1201

  Fragments arranged by size: 

                1201     537     465     301     292      81
 
AhdI GACnn_n'nnGTC

Cuts at:      0     415    2877 
   Size:        415    2462
 
AleI CACnn'nnGTG

Cuts at:      0    1880    2877 
   Size:       1880     997
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     850    2877 
   Size:        850    2027
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2505    2537    2877 
   Size:       2505      32     340

  Fragments arranged by size: 

                2505     340      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1353    1546    2877 
   Size:       1353     193    1331

  Fragments arranged by size: 

                1353    1331     193
 
ApaLI G'TGCA_C

Cuts at:      0     720    2877 
   Size:        720    2157
 
ApoI r'AATT_y

Cuts at:      0     840    1132    1204    2133    2388    2664    2761    2877 
   Size:        840     292      72     929     255     276      97     116

  Fragments arranged by size: 

                 929     840     292     276     255     116      97      72
            
 
BanI G'GyrC_C

Cuts at:      0     360    1025    2877 
   Size:        360     665    1852

  Fragments arranged by size: 

                1852     665     360
 
BanII G_rGCy'C

Cuts at:      0     146    1907    2579    2877 
   Size:        146    1761     672     298

  Fragments arranged by size: 

                1761     672     298     146
 
BbeI G_GCGC'C

Cuts at:      0    1029    2877 
   Size:       1029    1848
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1664    2063    2877 
   Size:       1664     399     814

  Fragments arranged by size: 

                1664     814     399
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1935    1969    2877 
   Size:       1935      34     908

  Fragments arranged by size: 

                1935     908      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1972    2877 
   Size:       1972     905
 
BclI T'GATC_A

Cuts at:      0    1428    2877 
   Size:       1428    1449
 
BfrBI ATG'CAT

Cuts at:      0     511    2048    2877 
   Size:        511    1537     829

  Fragments arranged by size: 

                1537     829     511
 
BglII A'GATC_T

Cuts at:      0     789    2604    2713    2877 
   Size:        789    1815     109     164

  Fragments arranged by size: 

                1815     789     164     109
 
Bme1580I G_kGCm'C

Cuts at:      0     724    2877 
   Size:        724    2153
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2017    2832    2877 
   Size:       2017     815      45

  Fragments arranged by size: 

                2017     815      45
 
BmtI G_CTAG'C

Cuts at:      0    1042    2877 
   Size:       1042    1835
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136    2692    2877 
   Size:        136    2556     185

  Fragments arranged by size: 

                2556     185     136
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1818    2877 
   Size:       1818    1059
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     522    2508    2877 
   Size:        522    1986     369

  Fragments arranged by size: 

                1986     522     369
 
BsaBI GATnn'nnATC

Cuts at:      0    2191    2786    2877 
   Size:       2191     595      91

  Fragments arranged by size: 

                2191     595      91
 
BsaHI Gr'CG_yC

Cuts at:      0    1026    1737    2624    2877 
   Size:       1026     711     887     253

  Fragments arranged by size: 

                1026     887     711     253
 
BsaWI w'CCGG_w

Cuts at:      0     171    2877 
   Size:        171    2706
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      30      60    2505    2535    2877 
   Size:         30      30    2445      30     342

  Fragments arranged by size: 

                2445     342      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57    1570    2877 
   Size:         57    1513    1307

  Fragments arranged by size: 

                1513    1307      57
 
BseYI C'CCAG_C

Cuts at:      0     206    2877 
   Size:        206    2671
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1142    2877 
   Size:        803     339    1735

  Fragments arranged by size: 

                1735     803     339
 
BsiHKAI G_wGCw'C

Cuts at:      0     470     724    1580    1907    2579    2877 
   Size:        470     254     856     327     672     298

  Fragments arranged by size: 

                 856     672     470     327     298     254
 
BsmI GAATG_Cn'

Cuts at:      0    1098    1230    1380    2050    2877 
   Size:       1098     132     150     670     827

  Fragments arranged by size: 

                1098     827     670     150     132
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2715    2877 
   Size:       2715     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     470     724    1580    1907    2579    2877 
   Size:        146     324     254     856     327     672     298

  Fragments arranged by size: 

                 856     672     327     324     298     254     146
 
BsrBI CCG'CTC

Cuts at:      0    1860    2877 
   Size:       1860    1017
 
BsrDI GCAATG_nn'

Cuts at:      0     798    2877 
   Size:        798    2079
 
BsrFI r'CCGG_y

Cuts at:      0    1028    2447    2877 
   Size:       1028    1419     430

  Fragments arranged by size: 

                1419    1028     430
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2348    2877 
   Size:       2348     529
 
BstBI TT'CG_AA

Cuts at:      0    2832    2877 
   Size:       2832      45
 
BstYI r'GATC_y

Cuts at:      0     789    2589    2604    2713    2877 
   Size:        789    1800      15     109     164

  Fragments arranged by size: 

                1800     789     164     109      15
 
BtgI C'CryG_G

Cuts at:      0     390     676    2877 
   Size:        390     286    2201

  Fragments arranged by size: 

                2201     390     286
 
BtsI GCAGTG_nn'

Cuts at:      0    1055    2349    2877 
   Size:       1055    1294     528

  Fragments arranged by size: 

                1294    1055     528
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     797     832    2877 
   Size:        797      35    2045

  Fragments arranged by size: 

                2045     797      35
 
DraI TTT'AAA

Cuts at:      0     302    2769    2877 
   Size:        302    2467     108

  Fragments arranged by size: 

                2467     302     108
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     928    2877 
   Size:        928    1949
 
EaeI y'GGCC_r

Cuts at:      0     729    2635    2877 
   Size:        729    1906     242

  Fragments arranged by size: 

                1906     729     242
 
EarI CTCTTCn'nnn_

Cuts at:      0      75     458     761    2877 
   Size:         75     383     303    2116

  Fragments arranged by size: 

                2116     383     303      75
 
EcoICRI GAG'CTC

Cuts at:      0    1905    2577    2877 
   Size:       1905     672     300

  Fragments arranged by size: 

                1905     672     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     301     838    1303    1595    1676    2692    2877 
   Size:        136     165     537     465     292      81    1016     185

  Fragments arranged by size: 

                1016     537     465     292     185     165     136      81
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      84     190     302    1882    1912    2877 
   Size:         84     106     112    1580      30     965

  Fragments arranged by size: 

                1580     965     112     106      84      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1721    2838    2877 
   Size:       1721    1117      39

  Fragments arranged by size: 

                1721    1117      39
 
EcoRI G'AATT_C

Cuts at:      0    2388    2877 
   Size:       2388     489
 
EcoRV GAT'ATC

Cuts at:      0    2154    2877 
   Size:       2154     723
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2626    2877 
   Size:       2626     251
 
HaeII r_GCGC'y

Cuts at:      0    1029    2877 
   Size:       1029    1848
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     401     433    1606    1636    1638    1668    2877 
   Size:        401      32    1173      30       2      30    1209

  Fragments arranged by size: 

                1209    1173     401      32      30      30       2
 
KasI G'GCGC_C

Cuts at:      0    1025    2877 
   Size:       1025    1852
 
MfeI C'AATT_G

Cuts at:      0     160    2877 
   Size:        160    2717
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1130    1368    2184    2877 
   Size:         73    1057     238     816     693

  Fragments arranged by size: 

                1057     816     693     238      73
 
MscI TGG'CCA

Cuts at:      0     731    2877 
   Size:        731    2146
 
MslI CAynn'nnrTG

Cuts at:      0      59     403    1330    1880    2877 
   Size:         59     344     927     550     997

  Fragments arranged by size: 

                 997     927     550     344      59
 
MspA1I CmG'CkG

Cuts at:      0     206     613     628    2877 
   Size:        206     407      15    2249

  Fragments arranged by size: 

                2249     407     206      15
 
NaeI GCC'GGC

Cuts at:      0    1030    2877 
   Size:       1030    1847
 
NarI GG'CG_CC

Cuts at:      0    1026    2877 
   Size:       1026    1851
 
NcoI C'CATG_G

Cuts at:      0     390    2877 
   Size:        390    2487
 
NdeI CA'TA_TG

Cuts at:      0    1938    2877 
   Size:       1938     939
 
NgoMIV G'CCGG_C

Cuts at:      0    1028    2877 
   Size:       1028    1849
 
NheI G'CTAG_C

Cuts at:      0    1038    2877 
   Size:       1038    1839
 
NsiI A_TGCA'T

Cuts at:      0     513    2050    2877 
   Size:        513    1537     827

  Fragments arranged by size: 

                1537     827     513
 
NspI r_CATG'y

Cuts at:      0     891    1748    2877 
   Size:        891     857    1129

  Fragments arranged by size: 

                1129     891     857
 
PasI CC'CwG_GG

Cuts at:      0    2842    2877 
   Size:       2842      35
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2029    2233    2415    2877 
   Size:       2029     204     182     462

  Fragments arranged by size: 

                2029     462     204     182
 
PfoI T'CCnGG_A

Cuts at:      0     329    1907    2877 
   Size:        329    1578     970

  Fragments arranged by size: 

                1578     970     329
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2505    2537    2877 
   Size:       2505      32     340

  Fragments arranged by size: 

                2505     340      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2838    2877 
   Size:       2838      39
 
PstI C_TGCA'G

Cuts at:      0     986    1874    2679    2877 
   Size:        986     888     805     198

  Fragments arranged by size: 

                 986     888     805     198
 
PvuII CAG'CTG

Cuts at:      0     206     613     628    2877 
   Size:        206     407      15    2249

  Fragments arranged by size: 

                2249     407     206      15
 
SacI G_AGCT'C

Cuts at:      0    1907    2579    2877 
   Size:       1907     672     298

  Fragments arranged by size: 

                1907     672     298
 
SapI GCTCTTCn'nnn_

Cuts at:      0     458    2877 
   Size:        458    2419
 
ScaI AGT'ACT

Cuts at:      0    2597    2877 
   Size:       2597     280
 
SexAI A'CCwGG_T

Cuts at:      0    2517    2877 
   Size:       2517     360
 
SfcI C'TryA_G

Cuts at:      0     982    1265    1870    2675    2877 
   Size:        982     283     605     805     202

  Fragments arranged by size: 

                 982     805     605     283     202
 
SfoI GGC'GCC

Cuts at:      0    1027    2877 
   Size:       1027    1850
 
SmlI C'TyrA_G

Cuts at:      0    1797    2877 
   Size:       1797    1080
 
SphI G_CATG'C

Cuts at:      0    1748    2877 
   Size:       1748    1129
 
SspI AAT'ATT

Cuts at:      0    1449    1683    2877 
   Size:       1449     234    1194

  Fragments arranged by size: 

                1449    1194     234
 
StyI C'CwwG_G

Cuts at:      0     191     390    2028    2877 
   Size:        191     199    1638     849

  Fragments arranged by size: 

                1638     849     199     191
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2118    2877 
   Size:       2118     759
 
TatI w'GTAC_w

Cuts at:      0    2546    2595    2877 
   Size:       2546      49     282

  Fragments arranged by size: 

                2546     282      49
 
XmnI GAAnn'nnTTC

Cuts at:      0     851    1407    2877 
   Size:        851     556    1470

  Fragments arranged by size: 

                1470     851     556
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII Bme1580I     BmrI     BmtI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI    BstBI
    BstYI     BtgI     BtsI    CspCI     DraI     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI     PasI
    PflMI     PfoI     PpiI    PpuMI     PstI    PvuII     SacI     SapI
     ScaI    SexAI     SfcI     SfoI     SmlI     SphI     SspI     StyI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BglI     BlpI    BmgBI     BplI   Bpu10I    BsaAI    BsiEI
    BsiWI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     EagI     EciI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MluI
     NotI     NruI     PacI     PciI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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