Restriction map for pF1KB3230
 (Linear) (Six-base) MAPSORT of: KIBB3230.seq  Check: 7386  from: 1  to: 1887



KIBB3230.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     181    1887 
   Size:        181    1706
 
AatII G_ACGT'C

Cuts at:      0    1778    1887 
   Size:       1778     109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1396    1887 
   Size:        865     531     491

  Fragments arranged by size: 

                 865     531     491
 
AflIII A'CryG_T

Cuts at:      0     633    1887 
   Size:        633    1254
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1588    1620    1887 
   Size:       1588      32     267

  Fragments arranged by size: 

                1588     267      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      86    1887 
   Size:         86    1801
 
ApaI G_GGCC'C

Cuts at:      0     281    1887 
   Size:        281    1606
 
ApaLI G'TGCA_C

Cuts at:      0    1234    1887 
   Size:       1234     653
 
AvaI C'yCGr_G

Cuts at:      0     986    1887 
   Size:        986     901
 
BamHI G'GATC_C

Cuts at:      0     372    1350    1887 
   Size:        372     978     537

  Fragments arranged by size: 

                 978     537     372
 
BanI G'GyrC_C

Cuts at:      0    1252    1887 
   Size:       1252     635
 
BanII G_rGCy'C

Cuts at:      0      47     218     281    1058    1133    1847    1887 
   Size:         47     171      63     777      75     714      40

  Fragments arranged by size: 

                 777     714     171      75      63      47      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     400    1330    1887 
   Size:        400     930     557

  Fragments arranged by size: 

                 930     557     400
 
BbvCI CC'TCA_GC

Cuts at:      0     193    1887 
   Size:        193    1694
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     426    1887 
   Size:        426    1461
 
BclI T'GATC_A

Cuts at:      0     855    1887 
   Size:        855    1032
 
BfrBI ATG'CAT

Cuts at:      0     649    1887 
   Size:        649    1238
 
BlpI GC'TnA_GC

Cuts at:      0     773    1887 
   Size:        773    1114
 
Bme1580I G_kGCm'C

Cuts at:      0     169     281    1238    1887 
   Size:        169     112     957     649

  Fragments arranged by size: 

                 957     649     169     112
 
BmgBI CAC'GTC

Cuts at:      0     655    1887 
   Size:        655    1232
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1644    1850    1887 
   Size:       1644     206      37

  Fragments arranged by size: 

                1644     206      37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     918    1072    1887 
   Size:        918     154     815

  Fragments arranged by size: 

                 918     815     154
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      61     616    1117    1887 
   Size:         31      30     555     501     770

  Fragments arranged by size: 

                 770     555     501      31      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     193    1887 
   Size:        193    1694
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1887 
   Size:        202    1685
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289     592    1125    1887 
   Size:        289     303     533     762

  Fragments arranged by size: 

                 762     533     303     289
 
BsaHI Gr'CG_yC

Cuts at:      0     320    1775    1887 
   Size:        320    1455     112

  Fragments arranged by size: 

                1455     320     112
 
BsaWI w'CCGG_w

Cuts at:      0     495    1150    1887 
   Size:        495     655     737

  Fragments arranged by size: 

                 737     655     495
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     691    1887 
   Size:        691    1196
 
BseYI C'CCAG_C

Cuts at:      0     289    1887 
   Size:        289    1598
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1044    1854    1887 
   Size:       1044     810      33

  Fragments arranged by size: 

                1044     810      33
 
BsiHKAI G_wGCw'C

Cuts at:      0      47    1058    1133    1238    1887 
   Size:         47    1011      75     105     649

  Fragments arranged by size: 

                1011     649     105      75      47
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     169     218     281    1058    1133    1238    1847 
   Size:         47     122      49      63     777      75     105     609

Cuts at:   1847    1887 
   Size:         40

  Fragments arranged by size: 

                 777     609     122     105      75      63      49      47
                  40
 
BspEI T'CCGG_A

Cuts at:      0     495    1887 
   Size:        495    1392
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      34     181    1887 
   Size:         34     147    1706

  Fragments arranged by size: 

                1706     147      34
 
BsrBI CCG'CTC

Cuts at:      0     275     309     807    1250    1887 
   Size:        275      34     498     443     637

  Fragments arranged by size: 

                 637     498     443     275      34
 
BsrFI r'CCGG_y

Cuts at:      0     176    1502    1887 
   Size:        176    1326     385

  Fragments arranged by size: 

                1326     385     176
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     797    1242    1887 
   Size:        797     445     645

  Fragments arranged by size: 

                 797     645     445
 
BstYI r'GATC_y

Cuts at:      0     372    1350    1887 
   Size:        372     978     537

  Fragments arranged by size: 

                 978     537     372
 
BtgI C'CryG_G

Cuts at:      0     905    1887 
   Size:        905     982
 
BtsI GCAGTG_nn'

Cuts at:      0    1311    1887 
   Size:       1311     576
 
DraI TTT'AAA

Cuts at:      0    1344    1887 
   Size:       1344     543
 
DraIII CAC_nnn'GTG

Cuts at:      0     124    1814    1887 
   Size:        124    1690      73

  Fragments arranged by size: 

                1690     124      73
 
EaeI y'GGCC_r

Cuts at:      0    1500    1887 
   Size:       1500     387
 
EarI CTCTTCn'nnn_

Cuts at:      0      65    1046    1423    1887 
   Size:         65     981     377     464

  Fragments arranged by size: 

                 981     464     377      65
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     327    1887 
   Size:        327    1560
 
EcoICRI GAG'CTC

Cuts at:      0      45    1056    1131    1887 
   Size:         45    1011      75     756

  Fragments arranged by size: 

                1011     756      75      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      61     616     865    1117    1396    1887 
   Size:         31      30     555     249     252     279     491

  Fragments arranged by size: 

                 555     491     279     252     249      31      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     348    1887 
   Size:         54     294    1539

  Fragments arranged by size: 

                1539     294      54
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     480     567    1281    1887 
   Size:        480      87     714     606

  Fragments arranged by size: 

                 714     606     480      87
 
FspI TGC'GCA

Cuts at:      0      75    1787    1887 
   Size:         75    1712     100

  Fragments arranged by size: 

                1712     100      75
 
HaeII r_GCGC'y

Cuts at:      0    1615    1887 
   Size:       1615     272
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1342    1374    1402    1405    1434    1437    1708    1740 
   Size:       1342      32      28       3      29       3     271      32

Cuts at:   1740    1887 
   Size:        147

  Fragments arranged by size: 

                1342     271     147      32      32      29      28       3
                   3
 
HincII GTy'rAC

Cuts at:      0     631    1887 
   Size:        631    1256
 
MslI CAynn'nnrTG

Cuts at:      0     536    1084    1267    1887 
   Size:        536     548     183     620

  Fragments arranged by size: 

                 620     548     536     183
 
MspA1I CmG'CkG

Cuts at:      0    1622    1881    1887 
   Size:       1622     259       6

  Fragments arranged by size: 

                1622     259       6
 
NaeI GCC'GGC

Cuts at:      0     178    1504    1887 
   Size:        178    1326     383

  Fragments arranged by size: 

                1326     383     178
 
NcoI C'CATG_G

Cuts at:      0     905    1887 
   Size:        905     982
 
NdeI CA'TA_TG

Cuts at:      0    1071    1887 
   Size:       1071     816
 
NgoMIV G'CCGG_C

Cuts at:      0     176    1502    1887 
   Size:        176    1326     385

  Fragments arranged by size: 

                1326     385     176
 
NsiI A_TGCA'T

Cuts at:      0     651    1887 
   Size:        651    1236
 
NspI r_CATG'y

Cuts at:      0     184     637    1887 
   Size:        184     453    1250

  Fragments arranged by size: 

                1250     453     184
 
PciI A'CATG_T

Cuts at:      0     633    1887 
   Size:        633    1254
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     348    1887 
   Size:         54     294    1539

  Fragments arranged by size: 

                1539     294      54
 
PshAI GACnn'nnGTC

Cuts at:      0     400    1887 
   Size:        400    1487
 
PspOMI G'GGCC_C

Cuts at:      0     277    1887 
   Size:        277    1610
 
PstI C_TGCA'G

Cuts at:      0    1247    1887 
   Size:       1247     640
 
PvuII CAG'CTG

Cuts at:      0    1881    1887 
   Size:       1881       6
 
SacI G_AGCT'C

Cuts at:      0      47    1058    1133    1887 
   Size:         47    1011      75     754

  Fragments arranged by size: 

                1011     754      75      47
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1046    1887 
   Size:       1046     841
 
SfcI C'TryA_G

Cuts at:      0     235     871    1243    1887 
   Size:        235     636     372     644

  Fragments arranged by size: 

                 644     636     372     235
 
SmlI C'TyrA_G

Cuts at:      0     217    1887 
   Size:        217    1670
 
StyI C'CwwG_G

Cuts at:      0     905    1887 
   Size:        905     982
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1626    1887 
   Size:       1626     261
 
TatI w'GTAC_w

Cuts at:      0    1136    1259    1887 
   Size:       1136     123     628

  Fragments arranged by size: 

                1136     628     123
 
XbaI T'CTAG_A

Cuts at:      0     207    1887 
   Size:        207    1680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138    1465    1887 
   Size:        138    1327     422

  Fragments arranged by size: 

                1327     422     138
 
ZraI GAC'GTC

Cuts at:      0    1776    1887 
   Size:       1776     111
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AloI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BstAPI    BstYI     BtgI
     BtsI     DraI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI     SapI     SfcI     SmlI
     StyI    TaqII     TatI     XbaI     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI     BglI    BglII     BmtI    BsaAI    BsaBI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XhoI     XmaI     XmnI
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