Restriction map for
pF1KB3118
(Linear) (Six-base) MAPSORT of: KIBB3118.seq Check: 5938 from: 1 to: 2544
KIBB3118.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 799 953 1698 1772 2464 2544
Size: 104 695 154 745 74 692 80
Fragments arranged by size:
745 695 692 154 104 80 74
AflIII A'CryG_T
Cuts at: 0 2287 2544
Size: 2287 257
AleI CACnn'nnGTG
Cuts at: 0 2344 2544
Size: 2344 200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 960 2544
Size: 960 1584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2086 2118 2544
Size: 2086 32 426
Fragments arranged by size:
2086 426 32
AlwNI CAG_nnn'CTG
Cuts at: 0 363 556 2544
Size: 363 193 1988
Fragments arranged by size:
1988 363 193
ApaLI G'TGCA_C
Cuts at: 0 945 2544
Size: 945 1599
ApoI r'AATT_y
Cuts at: 0 312 346 505 711 849 1678 2544
Size: 312 34 159 206 138 829 866
Fragments arranged by size:
866 829 312 206 159 138 34
AseI AT'TA_AT
Cuts at: 0 182 2544
Size: 182 2362
AvrII C'CTAG_G
Cuts at: 0 1635 2544
Size: 1635 909
BamHI G'GATC_C
Cuts at: 0 1272 2544
Size: 1272 1272
BanII G_rGCy'C
Cuts at: 0 1589 2544
Size: 1589 955
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1128 1658 2544
Size: 500 628 530 886
Fragments arranged by size:
886 628 530 500
BbvCI CC'TCA_GC
Cuts at: 0 2333 2544
Size: 2333 211
BlpI GC'TnA_GC
Cuts at: 0 214 2544
Size: 214 2330
Bme1580I G_kGCm'C
Cuts at: 0 949 2544
Size: 949 1595
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 2544
Size: 534 2010
BmtI G_CTAG'C
Cuts at: 0 155 2544
Size: 155 2389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1049 1244 1592 2544
Size: 611 438 195 348 952
Fragments arranged by size:
952 611 438 348 195
Bpu10I CC'TnA_GC
Cuts at: 0 957 2333 2544
Size: 957 1376 211
Fragments arranged by size:
1376 957 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1809 2544
Size: 1809 735
BsaI GGTCTCn'nnnn_
Cuts at: 0 113 589 1143 2544
Size: 113 476 554 1401
Fragments arranged by size:
1401 554 476 113
BsaAI yAC'GTr
Cuts at: 0 2346 2544
Size: 2346 198
BsaWI w'CCGG_w
Cuts at: 0 839 2544
Size: 839 1705
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1217 1247 2544
Size: 1217 30 1297
Fragments arranged by size:
1297 1217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2270 2544
Size: 2270 274
BseYI C'CCAG_C
Cuts at: 0 78 2296 2544
Size: 78 2218 248
Fragments arranged by size:
2218 248 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1418 1484 2544
Size: 76 1342 66 1060
Fragments arranged by size:
1342 1060 76 66
BsiHKAI G_wGCw'C
Cuts at: 0 949 2544
Size: 949 1595
BsmI GAATG_Cn'
Cuts at: 0 1480 2544
Size: 1480 1064
Bsp1286I G_dGCh'C
Cuts at: 0 949 1589 2544
Size: 949 640 955
Fragments arranged by size:
955 949 640
BspEI T'CCGG_A
Cuts at: 0 839 2544
Size: 839 1705
BsrDI GCAATG_nn'
Cuts at: 0 713 2544
Size: 713 1831
BsrFI r'CCGG_y
Cuts at: 0 51 2544
Size: 51 2493
BsrGI T'GTAC_A
Cuts at: 0 547 1616 2140 2544
Size: 547 1069 524 404
Fragments arranged by size:
1069 547 524 404
BssSI C'ACGA_G
Cuts at: 0 948 2544
Size: 948 1596
BstEII G'GTnAC_C
Cuts at: 0 1576 2544
Size: 1576 968
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2248 2544
Size: 2248 296
BstYI r'GATC_y
Cuts at: 0 1272 2544
Size: 1272 1272
Bsu36I CC'TnA_GG
Cuts at: 0 1550 2544
Size: 1550 994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 767 802 2544
Size: 767 35 1742
Fragments arranged by size:
1742 767 35
DraI TTT'AAA
Cuts at: 0 2396 2420 2544
Size: 2396 24 124
Fragments arranged by size:
2396 124 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 2544
Size: 60 2484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 611 799 953 1049 1244 1592 1698
Size: 104 507 188 154 96 195 348 106
Cuts at: 1698 1772 2464 2544
Size: 74 692 80
Fragments arranged by size:
692 507 348 195 188 154 106 104
96 80 74
EcoRI G'AATT_C
Cuts at: 0 346 2544
Size: 346 2198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 1794 2544
Size: 261 1533 750
Fragments arranged by size:
1533 750 261
HaeII r_GCGC'y
Cuts at: 0 17 2544
Size: 17 2527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 792 824 1546 1578 2512 2544
Size: 676 32 84 32 722 32 934 32
Fragments arranged by size:
934 722 676 84 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1710 2544
Size: 1710 834
MfeI C'AATT_G
Cuts at: 0 1986 2544
Size: 1986 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 170 2544
Size: 119 51 2374
Fragments arranged by size:
2374 119 51
MslI CAynn'nnrTG
Cuts at: 0 467 2344 2544
Size: 467 1877 200
Fragments arranged by size:
1877 467 200
MspA1I CmG'CkG
Cuts at: 0 82 982 1911 2437 2544
Size: 82 900 929 526 107
Fragments arranged by size:
929 900 526 107 82
NdeI CA'TA_TG
Cuts at: 0 661 2544
Size: 661 1883
NheI G'CTAG_C
Cuts at: 0 151 2544
Size: 151 2393
NspI r_CATG'y
Cuts at: 0 1668 1935 1950 2291 2544
Size: 1668 267 15 341 253
Fragments arranged by size:
1668 341 267 253 15
PasI CC'CwG_GG
Cuts at: 0 1582 2544
Size: 1582 962
PciI A'CATG_T
Cuts at: 0 2287 2544
Size: 2287 257
PmlI CAC'GTG
Cuts at: 0 2346 2544
Size: 2346 198
PshAI GACnn'nnGTC
Cuts at: 0 598 1084 1750 2544
Size: 598 486 666 794
Fragments arranged by size:
794 666 598 486
PsiI TTA'TAA
Cuts at: 0 608 1112 2544
Size: 608 504 1432
Fragments arranged by size:
1432 608 504
PvuII CAG'CTG
Cuts at: 0 82 982 1911 2437 2544
Size: 82 900 929 526 107
Fragments arranged by size:
929 900 526 107 82
ScaI AGT'ACT
Cuts at: 0 1422 1431 2372 2544
Size: 1422 9 941 172
Fragments arranged by size:
1422 941 172 9
SfcI C'TryA_G
Cuts at: 0 300 389 2544
Size: 300 89 2155
Fragments arranged by size:
2155 300 89
SmlI C'TyrA_G
Cuts at: 0 1824 2544
Size: 1824 720
SpeI A'CTAG_T
Cuts at: 0 1303 2544
Size: 1303 1241
StuI AGG'CCT
Cuts at: 0 326 2544
Size: 326 2218
StyI C'CwwG_G
Cuts at: 0 717 1635 1811 2544
Size: 717 918 176 733
Fragments arranged by size:
918 733 717 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 512 1595 2356 2544
Size: 137 375 1083 761 188
Fragments arranged by size:
1083 761 375 188 137
TatI w'GTAC_w
Cuts at: 0 547 1420 1429 1616 2140 2360 2370 2544
Size: 547 873 9 187 524 220 10 174
Fragments arranged by size:
873 547 524 220 187 174 10 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1217 1249 2544
Size: 1217 32 1295
Fragments arranged by size:
1295 1217 32
XbaI T'CTAG_A
Cuts at: 0 1854 2544
Size: 1854 690
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI
AseI AvrII BamHI BanII BbsI BbvCI BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
CspCI DraI EciI Eco57MI EcoRI FalI HaeII Hin4I
HincII MfeI MmeI MslI MspA1I NdeI NheI NspI
PasI PciI PmlI PshAI PsiI PvuII ScaI SfcI
SmlI SpeI StuI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI AscI AsiSI AvaI BaeI BanI BbeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BplI
BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BspMI BsrBI
BssHII BstAPI BstBI BstZ17I BtgI BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NotI NruI
NsiI PacI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI SwaI Tth111I XcmI
XhoI XmaI XmnI ZraI