Restriction map for pF1KB3106
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AflIII      
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BciVI       BclI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaHI       
BsaWI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
FbaI        FseI        FspI        HgaI        Hin1I       
HpaI        KasI        KspI        MluI        MroI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
RsrII       SacI        SacII       SalI        SapI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       2045  GGTACC               G^GTACC
AccI          832  GTCTAC               GT^[AC][GT]AC
AccII         945  CGCG                 CG^CG
AcsI         2107  AAATTT               [GA]^AATT[CT]
Alw44I       1534  GTGCAC               G^TGCAC
AocI          331  CCTAAGG              CC^T[ACGT]AGG
ApaI          209  GGGCCC               GGGCC^C
ApaLI        1534  GTGCAC               G^TGCAC
ApoI         2107  AAATTT               [GA]^AATT[CT]
Asp718I      2045  GGTACC               G^GTACC
BcgI         1752  CGACTCCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          663  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      663  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2424  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2332  GAGACC               GGTCTC(1/5)
BsgI         1332  CTGCAC               GTGCAG(16/14)
BspMI         968  ACCTGC               ACCTGC(4/8)
BsrDI        2190  CATTGC               GCAATG(2/0)
BsrFI        1016  ACCGGC               [GA]^CCGG[CT]
BssSI        1093  CACGAG               CACGAG(-5/-1)
BstAPI        963  GCACAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         945  CGCG                 CG^CG
Bsu36I        331  CCTAAGG              CC^T[ACGT]AGG
CelII         663  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1016  ACCGGC               [GA]^CCGG[CT]
DrdI         1039  GACATGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        331  CCTAAGG              CC^T[ACGT]AGG
EcoRV        2125  GATATC               GAT^ATC
EcoT22I      1898  ATGCAT               ATGCA^T
HindIII       463  AAGCTT               A^AGCTT
KpnI         2045  GGTACC               GGTAC^C
MamI         2424  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          533  CAATTG               C^AATTG
MunI          533  CAATTG               C^AATTG
MvnI          945  CGCG                 CG^CG
NheI         2296  GCTAGC               G^CTAGC
NsiI         1898  ATGCAT               ATGCA^T
NspI         2165  ACATGC               [GA]CATG^[CT]
PshAI        1077  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1218  CCTGCAGG             CCTGCA^GG
Sse8387I     1218  CCTGCAGG             CCTGCA^GG
SspI         1471  AATATT               AAT^ATT
TfiI          289  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AclI          868  AACGTT               AA^CGTT
             2152  AACGTT              
Asp700I        96  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2220  GAAGGACTTC          
AvaI          502  CTCGGG               C^[CT]CG[GA]G
              952  CCCGAG              
BalI         1274  TGGCCA               TGG^CCA
             2504  TGGCCA              
BanII         209  GGGCCC               G[GA]GC[CT]^C
              250  GGGCTC              
BbvCI        1116  CCTCAGC              CCTCAGC(-5/-2)
             2341  GCTGAGG             
BsiEI        1254  CGACCG               CG[GA][CT]^CG
             2433  CGACCG              
BsoBI         502  CTCGGG               C^[CT]CG[GA]G
              952  CCCGAG              
BspHI        1292  TCATGA               T^CATGA
             2129  TCATGA              
BstBI        1210  TTCGAA               TT^CGAA
             1466  TTCGAA              
DraIII       1479  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2037  CACCAAGTG           
HaeII         119  AGCGCC               [GA]GCGC^[CT]
             1726  AGCGCC              
MluNI        1274  TGGCCA               TGG^CCA
             2504  TGGCCA              
MscI         1274  TGGCCA               TGG^CCA
             2504  TGGCCA              
NcoI         1398  CCATGG               C^CATGG
             1660  CCATGG              
NspV         1210  TTCGAA               TT^CGAA
             1466  TTCGAA              
Psp1406I      868  AACGTT               AA^CGTT
             2152  AACGTT              
RcaI         1292  TCATGA               T^CATGA
             2129  TCATGA              
ScaI         1229  AGTACT               AGT^ACT
             1301  AGTACT              
SfuI         1210  TTCGAA               TT^CGAA
             1466  TTCGAA              
SmlI          253  CTCAAG               C^T[CT][GA]AG
              791  CTCAAG              
XcmI          407  CCAAATCTTTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2039  CCAAGTGGTACCTGG     
XmnI           96  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2220  GAAGGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BamHI       BanI        BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com