Restriction map for pF1KB3048
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        AscI        
Asp700I     AspEI       AspI        AvrII       BbeI        
BbrPI       BclI        BfrI        BglII       BlnI        
BsmBI       BspHI       BsrDI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoO65I     EcoT22I     FbaI        
FseI        HpaI        KasI        KspI        MfeI        
MluI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        1756  GACGTC               GACGT^C
AccII        2994  CGCG                 CG^CG
Alw44I       2169  GTGCAC               G^TGCAC
AlwNI        2931  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1746  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       732  AGCGCT               AGC^GCT
ApaLI        2169  GTGCAC               G^TGCAC
AvaI         1223  CCCGGG               C^[CT]CG[GA]G
AviII        2005  TGCGCA               TGC^GCA
BamHI         996  GGATCC               G^GATCC
BbvCI        3727  GCTGAGG              CCTCAGC(-5/-2)
BcgI          967  CGATGTACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         3583  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3583  GCTCAGC              GC^T[ACGT]AGC
BsaI         2330  GAGACC               GGTCTC(1/5)
BsiEI        3385  CGACCG               CG[GA][CT]^CG
BsiWI        2581  CGTACG               C^GTACG
BsoBI        1223  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2983  TGTACA               T^GTACA
BspDI         965  ATCGAT               AT^CGAT
BsrGI        2983  TGTACA               T^GTACA
BstUI        2994  CGCG                 CG^CG
BstXI        3350  CCATGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1746  CCTGAGG              CC^T[ACGT]AGG
CelII        3583  GCTCAGC              GC^T[ACGT]AGC
ClaI          965  ATCGAT               AT^CGAT
Eco47III      732  AGCGCT               AGC^GCT
Eco81I       1746  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1905  GAATTC               G^AATTC
FspI         2005  TGCGCA               TGC^GCA
HaeII         732  AGCGCT               [GA]GCGC^[CT]
MvnI         2994  CGCG                 CG^CG
NdeI         3623  CATATG               CA^TATG
SapI         1195  GAAGAGC              GCTCTTC(1/4)
SfiI          535  GGCCCAACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1223  CCCGGG               CCC^GGG
SphI         3539  GCATGC               GCATG^C
SspBI        2983  TGTACA               T^GTACA
XcmI          702  CCAGGAAACCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1223  CCCGGG               C^CCGGG
XmaI         1223  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII       2076  TCCGGA               T^CCGGA
             2427  TCCGGA              
ApaI         1051  GGGCCC               GGGCC^C
             1152  GGGCCC              
AspHI        2169  GTGCAC               G[AT]GC[AT]^C
             2252  GTGCTC              
BglI          536  GCCCAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2054  GCCACAAAGGC         
BsaBI        2875  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3808  GATGGGTATC          
BseAI        2076  TCCGGA               T^CCGGA
             2427  TCCGGA              
BsiHKAI      2169  GTGCAC               G[AT]GC[AT]^C
             2252  GTGCTC              
BspEI        2076  TCCGGA               T^CCGGA
             2427  TCCGGA              
BspLU11I     2042  ACATGT               A^CATGT
             2458  ACATGT              
BsrBI          46  CCGCTC               CCGCTC(-3/-3)
             2844  CCGCTC              
Bst1107I     3380  GTATAC               GTA^TAC
             3660  GTATAC              
BstZ17I      3380  GTATAC               GTA^TAC
             3660  GTATAC              
EarI         1042  GAAGAG               CTCTTC(1/4)
             1195  GAAGAG              
EcoRV        1507  GATATC               GAT^ATC
             2344  GATATC              
HindIII       497  AAGCTT               A^AGCTT
             1430  AAGCTT              
Ksp632I      1042  GAAGAG               CTCTTC(1/4)
             1195  GAAGAG              
MamI         2875  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3808  GATGGGTATC          
MroI         2076  TCCGGA               T^CCGGA
             2427  TCCGGA              
NaeI         3473  GCCGGC               GCC^GGC
             3614  GCCGGC              
NgoMIV       3473  GCCGGC               G^CCGGC
             3614  GCCGGC              
PpuMI        2407  GGGTCCT              [GA]G^G[AT]CC[CT]
             2782  GGGTCCT             
SmlI         1315  CTTGAG               C^T[CT][GA]AG
             1469  CTCAAG              
StuI         1145  AGGCCT               AGG^CCT
             3530  AGGCCT              
TaqI          966  TCGA                 T^CGA
             3845  TCGA                
TthHB8I       966  TCGA                 T^CGA
             3845  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

Acc65I      AccI        AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        ApoI        
AseI        AsnI        Asp718I     AvaII       BalI        
BanI        BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KpnI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PshAI       PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
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