Restriction map for pF1KB3043
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BciVI       BfrI        BlnI        
BsaBI       BsgI        BsiWI       BsmBI       BspDI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EcoT22I     FseI        
FspI        HgaI        HindIII     KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmeI        
PshBI       Psp1406I    RsrII       SacI        SacII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Van91I      
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        587  TCCGGA               T^CCGGA
AhdI           33  GACCTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        325  GTGCAC               G^TGCAC
ApaLI         325  GTGCAC               G^TGCAC
AspEI          33  GACCTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1417  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1613  TGGCCA               TGG^CCA
BfaI          757  CTAG                 C^TAG
BseAI         587  TCCGGA               T^CCGGA
BsmI          741  GAATGC               GAATGC(1/-1)
Bsp1407I     3380  TGTACA               T^GTACA
BspEI         587  TCCGGA               T^CCGGA
BspHI        2357  TCATGA               T^CATGA
BspLU11I      257  ACATGT               A^CATGT
BspMI         234  GCAGGT               ACCTGC(4/8)
BsrBI        3319  CCGCTC               CCGCTC(-3/-3)
BsrFI        2757  GCCGGT               [GA]^CCGG[CT]
BsrGI        3380  TGTACA               T^GTACA
Cfr10I       2757  GCCGGT               [GA]^CCGG[CT]
DraIII       1757  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2046  CGGCCG               C^GGCCG
Eam1105I       33  GACCTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2046  CGGCCG               C^GGCCG
Eco52I       2046  CGGCCG               C^GGCCG
EcoNI        1484  CCTTGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1274  GATATC               GAT^ATC
HincII         21  GTTAAC               GT[CT]^[GA]AC
HindII         21  GTTAAC               GT[CT]^[GA]AC
HpaI           21  GTTAAC               GTT^AAC
MaeI          757  CTAG                 C^TAG
MluNI        1613  TGGCCA               TGG^CCA
MroI          587  TCCGGA               T^CCGGA
MscI         1613  TGGCCA               TGG^CCA
NcoI         2793  CCATGG               C^CATGG
NdeI         2943  CATATG               CA^TATG
PaeR7I       2982  CTCGAG               C^TCGAG
PflFI        1417  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI         1001  CGATCG               CGAT^CG
RcaI         2357  TCATGA               T^CATGA
SbfI          231  CCTGCAGG             CCTGCA^GG
SexAI        1418  ACCTGGT              A^CC[AT]GGT
SfiI         1614  GGCCATTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      231  CCTGCAGG             CCTGCA^GG
SspBI        3380  TGTACA               T^GTACA
Tth111I      1417  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2982  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII        2062  CGCG                 CG^CG
             3375  CGCG                
AcyI         1360  GGCGCC               G[GA]^CG[CT]C
             2528  GGCGCC              
AocI          817  CCTGAGG              CC^T[ACGT]AGG
             1673  CCTCAGG             
BamHI         127  GGATCC               G^GATCC
              379  GGATCC              
BbeI         1360  GGCGCC               GGCGC^C
             2528  GGCGCC              
BbrPI         931  CACGTG               CAC^GTG
             1822  CACGTG              
BbsI          963  GAAGAC               GAAGAC(2/6)
             2068  GTCTTC              
BbvCI         298  CCTCAGC              CCTCAGC(-5/-2)
             2337  CCTCAGC             
BcgI         1349  GCACCCAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3332  GCAGCTCTGTCG        
BclI          927  TGATCA               T^GATCA
             1917  TGATCA              
BglI         1615  GCCATTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2919  GCCCGTCCGGC         
BglII         992  AGATCT               A^GATCT
             3212  AGATCT              
BlpI         1095  GCTGAGC              GC^T[ACGT]AGC
             3034  GCTGAGC             
Bpu1102I     1095  GCTGAGC              GC^T[ACGT]AGC
             3034  GCTGAGC             
BpuAI         963  GAAGAC               GAAGAC(2/6)
             2068  GTCTTC              
BsaHI        1360  GGCGCC               G[GA]^CG[CT]C
             2528  GGCGCC              
BsaWI         587  TCCGGA               [AT]^CCGG[AT]
             2483  ACCGGA              
BsiEI        1001  CGATCG               CG[GA][CT]^CG
             2046  CGGCCG              
BssSI         923  CTCGTG               CACGAG(-5/-1)
             2374  CTCGTG              
BstAPI       1821  GCACGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2582  GCACCAGCTGC         
BstUI        2062  CGCG                 CG^CG
             3375  CGCG                
Bsu36I        817  CCTGAGG              CC^T[ACGT]AGG
             1673  CCTCAGG             
CelII        1095  GCTGAGC              GC^T[ACGT]AGC
             3034  GCTGAGC             
Eco81I        817  CCTGAGG              CC^T[ACGT]AGG
             1673  CCTCAGG             
FbaI          927  TGATCA               T^GATCA
             1917  TGATCA              
Hin1I        1360  GGCGCC               G[GA]^CG[CT]C
             2528  GGCGCC              
KasI         1360  GGCGCC               G^GCGCC
             2528  GGCGCC              
MvnI         2062  CGCG                 CG^CG
             3375  CGCG                
NarI         1360  GGCGCC               GG^CGCC
             2528  GGCGCC              
PmaCI         931  CACGTG               CAC^GTG
             1822  CACGTG              
PmlI          931  CACGTG               CAC^GTG
             1822  CACGTG              
PshAI        2103  GACACAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2445  GACACAGGTC          
PstI          232  CTGCAG               CTGCA^G
             2589  CTGCAG              
SapI         1974  GAAGAGC              GCTCTTC(1/4)
             2577  GAAGAGC             
ScaI          572  AGTACT               AGT^ACT
             1848  AGTACT              
SfoI         1360  GGCGCC               GGC^GCC
             2528  GGCGCC              
SmaI         2390  CCCGGG               CCC^GGG
             3438  CCCGGG              
XmaCI        2390  CCCGGG               C^CCGGG
             3438  CCCGGG              
XmaI         2390  CCCGGG               C^CCGGG
             3438  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       Aor51HI     ApaI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco47III    
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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