Restriction map for pF1KB3030
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BlnI        BsaHI       BsiWI       BspHI       
BspLU11I    BspMI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       FseI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        XbaI        XcmI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII        1746  CGCG                 CG^CG
AflIII       1544  ACGTGT               A^C[GA][CT]GT
AgeI          991  ACCGGT               A^CCGGT
Alw44I       2466  GTGCAC               G^TGCAC
AlwNI        1447  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          343  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2466  GTGCAC               G^TGCAC
AspI         1969  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        2531  GGATAC               GTATCC(6/5)
BsaAI        1543  TACGTG               [CT]AC^GT[GA]
BsaBI         762  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         993  CGGTCG               CG[GA][CT]^CG
BsmBI         548  GAGACG               CGTCTC(1/5)
BsmI          394  GAATGC               GAATGC(1/-1)
Bsp1407I      899  TGTACA               T^GTACA
BspDI        2275  ATCGAT               AT^CGAT
BsrGI         899  TGTACA               T^GTACA
BstEII       1899  GGTGACC              G^GT[ACGT]ACC
BstPI        1899  GGTGACC              G^GT[ACGT]ACC
BstUI        1746  CGCG                 CG^CG
Bsu36I        343  CCTGAGG              CC^T[ACGT]AGG
ClaI         2275  ATCGAT               AT^CGAT
Eco81I        343  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1899  GGTGACC              G^GT[ACGT]ACC
EcoRV         598  GATATC               GAT^ATC
EcoT22I      2623  ATGCAT               ATGCA^T
HaeII        2703  GGCGCT               [GA]GCGC^[CT]
MamI          762  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         2456  TTAA                 T^TAA
MvnI         1746  CGCG                 CG^CG
NcoI         1361  CCATGG               C^CATGG
NsiI         2623  ATGCAT               ATGCA^T
NspI           83  ACATGC               [GA]CATG^[CT]
PaeR7I       1756  CTCGAG               C^TCGAG
PflFI        1969  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         991  ACCGGT               A^CCGGT
SalI         1234  GTCGAC               G^TCGAC
SapI         2298  GCTCTTC              GCTCTTC(1/4)
SgrAI        1924  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         899  TGTACA               T^GTACA
Tru9I        2456  TTAA                 T^TAA
Tth111I      1969  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1756  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII        566  TCCGGA               T^CCGGA
             1046  TCCGGA              
AcsI         1608  AAATTT               [GA]^AATT[CT]
             2170  GAATTT              
ApoI         1608  AAATTT               [GA]^AATT[CT]
             2170  GAATTT              
AviII        1156  TGCGCA               TGC^GCA
             2069  TGCGCA              
BalI          938  TGGCCA               TGG^CCA
             1106  TGGCCA              
BbsI         1413  GAAGAC               GAAGAC(2/6)
             2002  GAAGAC              
BclI          887  TGATCA               T^GATCA
             2231  TGATCA              
BfaI          487  CTAG                 C^TAG
             2152  CTAG                
BglI         1162  GCCACCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2479  GCCCGGCGGGC         
BlpI          969  GCTCAGC              GC^T[ACGT]AGC
             1434  GCTGAGC             
Bpu1102I      969  GCTCAGC              GC^T[ACGT]AGC
             1434  GCTGAGC             
BpuAI        1413  GAAGAC               GAAGAC(2/6)
             2002  GAAGAC              
BseAI         566  TCCGGA               T^CCGGA
             1046  TCCGGA              
BsgI           55  GTGCAG               GTGCAG(16/14)
             1355  GTGCAG              
BspEI         566  TCCGGA               T^CCGGA
             1046  TCCGGA              
BstAPI        701  GCAACGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2045  GCAATGCCTGC         
BstXI         530  CCAGTGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2493  CCAGCTCTGTGG        
CelII         969  GCTCAGC              GC^T[ACGT]AGC
             1434  GCTGAGC             
DrdI         1237  GACGGTTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1726  GACCACATTGTC        
DsaI         1361  CCATGG               C^C[GA][CT]GG
             2544  CCGTGG              
EcoNI         197  CCTTTGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1905  CCTGCTGAAGG         
FbaI          887  TGATCA               T^GATCA
             2231  TGATCA              
FspI         1156  TGCGCA               TGC^GCA
             2069  TGCGCA              
HgaI         2554  GACGC                GACGC(5/10)
             1275  GCGTC               
HincII         64  GTTGAC               GT[CT]^[GA]AC
             1234  GTCGAC              
HindII         64  GTTGAC               GT[CT]^[GA]AC
             1234  GTCGAC              
MaeI          487  CTAG                 C^TAG
             2152  CTAG                
MluNI         938  TGGCCA               TGG^CCA
             1106  TGGCCA              
MroI          566  TCCGGA               T^CCGGA
             1046  TCCGGA              
MscI          938  TGGCCA               TGG^CCA
             1106  TGGCCA              
PflMI         306  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1446  CCAGCTGCTGG         
PleI         1089  GACTC                GAGTC(4/5)
             1506  GACTC               
SacI           37  GAGCTC               GAGCT^C
             1717  GAGCTC              
SmaI          861  CCCGGG               CCC^GGG
             2205  CCCGGG              
TfiI          324  GAATC                G^A[AT]TC
              521  GATTC               
Tsp509I      1609  AATT                 ^AATT
             2171  AATT                
Van91I        306  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1446  CCAGCTGCTGG         
XmaCI         861  CCCGGG               C^CCGGG
             2205  CCCGGG              
XmaI          861  CCCGGG               C^CCGGG
             2205  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BglII       BmyI        BpmI        
BsaI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com