GENSCAN 1.0	Date run:  3-Nov-116	Time: 12:10:29

Sequence gi568815596f:74734581_74990938 : 256358 bp : 44.11% C+G : Isochore 2 (43 - 51 C+G%)

Parameter matrix: HumanIso.smat

Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

 1.01 Init +  20051  20270  220  1  1   82   84   164 0.955  14.19
 1.02 Term +  35145  35272  128  1  2   54   44   142 0.620   4.84
 1.03 PlyA +  36523  36528    6                               1.05

 2.00 Prom +  41042  41081   40                               0.34
 2.01 Init +  45954  46100  147  2  0   79   94   100 0.429   7.89
 2.02 Intr +  85246  85355  110  0  2   39   80    87 0.038   2.08
 2.03 Intr +  99379  99657  279  1  0   99   16   118 0.184   2.29
 2.04 Intr + 100000 100063   64  1  1   48  115    96 0.218   7.02
 2.05 Intr + 119713 119875  163  0  1  134   84   191 0.913  22.85
 2.06 Intr + 127415 127508   94  0  1   38   84    95 0.042   3.12
 2.07 Intr + 130723 130880  158  1  2   89  -46   120 0.357  -1.65
 2.08 Intr + 133056 133204  149  1  2  109   80   202 0.988  21.45
 2.09 Intr + 137720 137839  120  1  0   88   94   116 0.999  12.89
 2.10 Intr + 138696 138791   96  2  0   82   84    64 0.977   5.61
 2.11 Intr + 139264 139363  100  0  1   92   92   205 0.999  20.98
 2.12 Intr + 139686 139869  184  1  1   62   74   384 0.999  33.25
 2.13 Intr + 142586 142741  156  2  0  103   84   242 0.999  24.43
 2.14 Intr + 144108 144341  234  0  0  106   81   491 0.970  47.00
 2.15 Intr + 145685 145989  305  2  2   10   86   527 0.869  40.93
 2.16 Intr + 147131 147279  149  0  2   78   83   259 0.999  24.35
 2.17 Intr + 147540 147659  120  2  0  128   77   185 0.746  22.09
 2.18 Intr + 150914 151009   96  1  0   77   75   176 0.984  15.41
 2.19 Intr + 151714 151813  100  0  1  104   87   168 0.999  17.98
 2.20 Intr + 151910 152093  184  0  1   54   80   340 0.999  28.65
 2.21 Intr + 153323 153478  156  2  0   95   78   189 0.999  17.73
 2.22 Intr + 154665 154898  234  0  0   97   70   273 0.997  23.20
 2.23 Term + 156217 156361  145  1  1   92   42   201 0.889  13.28
 2.24 PlyA + 157723 157728    6                               1.05

 3.03 PlyA - 157897 157892    6                               1.05
 3.02 Term - 160554 160502   53  1  2   87   50    34 0.577  -2.91
 3.01 Init - 163735 163666   70  1  1  105   47    65 0.497   5.31
 3.00 Prom - 165507 165468   40                              -2.76

 4.12 PlyA - 166166 166161    6                               1.05
 4.11 Term - 172724 172576  149  0  2   89   44   100 0.276   3.76
 4.10 Intr - 174713 174584  130  2  1   16   59    88 0.301  -1.03
 4.09 Intr - 176723 176648   76  1  1  101   66    56 0.738   4.22
 4.08 Intr - 180958 180823  136  2  1   99  100   108 0.575  12.73
 4.07 Intr - 185675 185620   56  1  2   88    9    94 0.179   0.12
 4.06 Intr - 188048 187975   74  2  2   51   70    41 0.023  -3.20
 4.05 Intr - 192441 192359   83  1  2   76   94    46 0.232   3.36
 4.04 Intr - 193676 193597   80  1  2   92   47    44 0.079  -0.11
 4.03 Intr - 194159 194088   72  1  0   54  103    75 0.154   4.12
 4.02 Intr - 199029 198837  193  1  1  130    7    50 0.317  -0.35
 4.01 Init - 200166 200085   82  0  1   75   53    88 0.619   5.13
 4.00 Prom - 201945 201906   40                              -2.66

 5.00 Prom + 216174 216213   40                              -0.46
 5.01 Init + 224100 224312  213  2  0  102   62   126 0.810  10.28
 5.02 Intr + 224761 224845   85  0  1  119   15    70 0.827   2.19
 5.03 Intr + 225525 225566   42  1  0   96  113    46 0.819   6.11
 5.04 Term + 235049 235143   95  0  2   32   43   136 0.244   1.49
 5.05 PlyA + 235354 235359    6                               1.05

 6.02 PlyA - 235405 235400    6                               1.05
 6.01 Sngl - 238865 236580 2286  2  0   43   41   794 0.942  63.35

Suboptimal exons with probability > 0.800

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------



Predicted peptide sequence(s):

Predicted coding sequence(s):


>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_1|115_aa
MRANPTSFYIEEPNGAFYIADDGPARDVHEFHAHLCALSLRAGPAQDLGDPGQLDRLHTA
GVHDGGAATMWRRVTSQVHQFTHLLKAEDDDADEEDDEDEDDGTHLLRVTEGELN

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_1|348_bp
atgcgggcgaacccaacgtccttttacattgaagaacccaacggggccttttacattgcg
gatgatggaccggctcgtgacgtccatgaatttcacgcgcacctgtgtgcactgtccctg
agagccggtcctgcccaggaccttggtgaccctggccagcttgataggctgcacacggct
ggtgttcatgatggcggcgcagcgacgatgtggcggagagttaccagccaggtgcatcag
tttactcatctgttaaaagcagaggatgatgatgctgatgaagaggatgatgaagatgaa
gatgatggtacccatctcctcagggttacagaaggtgaattgaattga

>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_2|1180_aa
MAMTSRLAGLQVAPAGRLSQLVMREVHTVGQSASRRFKQLGSDENPGRQLSANYYKTGPD
TFKEHWSEMSRHHILLSVKPHNHPVRLPAPVSERPRPRAVSDDWLRHGGGRSVGAHTLPA
QPMGVRTSLIRRPAGRQPLNKPHCCMKLRRRSPGLPLATSCHPAKKIRGPEPRACESERM
IASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAV
KMLPTFVRSTPDGTARLKPSREQGTMMLMVTVMMMMIKIVDIVSASPSENPAHHPFLVEL
WPLSPAGLASDDDPPGAASPPRSPVVTDSADRVYPGLEKHGEFLALDLGGTNFRVLWVKV
TDNGLQKVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKDKKLPLGFTFSFP
CHQTKLDESFLVSWTKGFKSSGVEGRDVVALIRKAIQRRGDFDIDIVAVVNDTVGTMMTC
GYDDHNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGSLNDIRTEF
DQEIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFGGKLSPELLNTGRFETK
DISDIEGEKDGIRKAREVLMRLGLDPTQEDCVATHRICQIVSTRSASLCAATLAAVLQRI
KENKGEERLRSTIGVDGSVYKKHPHFAKRLHKTVRRLVPGCDVRFLRSEDGSGKGAAMVT
AVAYRLADQHRARQKTLEHLQLSHDQLLEVKRRMKVEMERGLSKETHASAPVKMLPTYVC
ATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKWGGVEMHNKIYAIPQEVMHGTGDELFDH
IVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEGEDVVTLL
KEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGSNACYMEEMRNVELVE
GEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNI
LIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILQHLGLESTCDDSI
IVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHE
TVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_2|3543_bp
atggccatgaccagcagactggctggattgcaggtggccccagcagggagactctcacag
ttggtgatgagagaagttcacactgtaggtcagtcagcatcaagaaggttcaagcagctg
ggaagtgatgaaaaccctggtaggcagctcagtgccaactactataaaacaggtcctgat
acttttaaggaacactggtctgagatgtctagacaccacatcctgctctcagttaagccc
cacaaccatcctgtgaggctgccggctccggtgtctgagcggccgcgcccgcgagccgtg
agcgatgattggctgcgccacggcggcgggcggtccgtgggcgcacacaccctccccgcg
cagccaatgggcgtgcgcacgtcactgatccggaggcccgcgggccggcagcccctcaat
aagccacattgttgcatgaaactccggcgcaggagtcccgggctgccgctggcaacatcg
tgtcacccagctaagaaaatccgcgggcccgagccacgcgcctgtgaatcggagaggatg
attgcctcgcatctgcttgcctacttcttcacggagctcaaccatgaccaagtgcagaag
gttgaccagtatctctaccacatgcgcctctctgatgagaccctcttggagatctctaag
cggttccgcaaggagatggagaaagggcttggagccaccactcaccctactgcagcagtg
aagatgctgcccacctttgtgaggtccactccagatgggacagccagacttaaaccctct
agagagcagggaaccatgatgctgatggtgacggtgatgatgatgatgataaaaattgta
gacatcgttagtgccagtccatcagaaaatcctgcacaccacccgttcttggtggagctg
tggcctctgagtccagctgggctggcctcggatgatgacccaccaggggcagcctcgcca
cctcggtcccctgtggtcactgattcagcggatcgcgtgtatcctggacttgagaaacac
ggagagttcctggctctggatcttggagggaccaacttccgtgtgctttgggtgaaagta
acggacaatgggctccagaaggtggagatggagaatcagatctatgccatccctgaggac
atcatgcgaggcagtggcacccagctgtttgaccacattgccgaatgcctggctaacttc
atggataagctacaaatcaaagacaagaagctcccactgggttttaccttctcgttcccc
tgccaccagactaaactagacgagagtttcctggtctcatggaccaagggattcaagtcc
agtggagtggaaggcagagacgttgtggctctgatccggaaggccatccagaggagaggg
gactttgatatcgacattgtggctgtggtgaatgacacagttgggaccatgatgacctgt
ggttatgatgaccacaactgtgagattggtctcattgtgggcacgggcagcaacgcctgc
tacatggaagagatgcgccacatcgacatggtggaaggcgatgaggggcggatgtgtatc
aatatggagtggggggccttcggggacgatggctcgctcaacgacattcgcactgagttt
gaccaggagattgacatgggctcactgaacccgggaaagcaactgtttgagaagatgatc
agtgggatgtacatgggggagctggtgaggcttatcctggtgaagatggccaaggaggag
ctgctctttggggggaagctcagcccagagcttctcaacaccggtcgctttgagaccaaa
gacatctcagacattgaaggggagaaggatggcatccggaaggcccgtgaggtcctgatg
cggttgggcctggacccgactcaggaggactgcgtggccactcaccggatctgccagatc
gtgtccacacgctccgccagcctgtgcgcagccaccctggccgccgtgctgcagcgcatc
aaggagaacaaaggcgaggagcggctgcgctctactattggggtcgacggttccgtctac
aagaaacacccccattttgccaagcgtctacataagaccgtgcggcggctggtgcccggc
tgcgatgtccgcttcctccgctccgaggatggcagtggcaaaggtgcagccatggtgaca
gcagtggcttaccggctggccgatcaacaccgtgcccgccagaagacattagagcatctg
cagctgagccatgaccagctgctggaggtcaagaggaggatgaaggtagaaatggagcga
ggtctgagcaaggagactcatgccagtgcccccgtcaagatgctgcccacctacgtgtgt
gctaccccggacggcacagagaaaggggacttcttggccttggaccttggaggaacaaat
ttccgggtcctgctggtccgtgttcggaatgggaagtggggtggagtggagatgcacaac
aagatctacgccatcccgcaggaggtcatgcacggcaccggggacgagctctttgaccac
attgtccagtgcatcgcggacttcctcgagtacatgggcatgaagggcgtgtccctgcct
ctgggttttaccttctccttcccctgccagcagaacagcctggacgagagcatcctcctc
aagtggacaaaaggcttcaaggcatctggctgcgagggcgaggacgtggtgaccctgctg
aaggaagcgatccaccggcgagaggagtttgacctggatgtggttgctgtggtgaacgac
acagtcggaactatgatgacctgtggctttgaagaccctcactgtgaagttggcctcatt
gttggcacgggcagcaatgcctgctacatggaggagatgcgcaacgtggaactggtggaa
ggagaagaggggcggatgtgtgtgaacatggaatggggggccttcggggacaatggatgc
ctagatgacttccgcacagaatttgatgtggctgtggatgagctttcactcaaccccggc
aagcagaggttcgagaaaatgatcagtggaatgtacctgggtgagattgtccgtaacatt
ctcatcgatttcaccaagcgtggactactcttccgaggccgcatctcagagcggctcaag
acaaggggcatctttgaaaccaagttcttgtctcagattgagagtgactgcctggccctg
ctgcaagtccgagccatcctgcaacacttagggcttgagagcacctgtgacgacagcatc
attgttaaggaggtgtgcactgtggtggcccggcgggcagcccagctctgtggcgcaggc
atggccgctgtggtggacaggatacgagaaaaccgtgggctggacgctctcaaagtgaca
gtgggtgtggatgggaccctctacaagctacatcctcactttgccaaagtcatgcatgag
acagtgaaggacctggctccgaaatgtgatgtgtctttcctgcagtcagaggatggcagc
gggaagggggcggcgctcatcactgctgtggcctgccgcatccgtgaggctggacagcga
tag

>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_3|40_aa
MATYPDPRIQLLKTKDISLQQDVYPSSNTVNYEICDMRYG

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_3|123_bp
atggccacctatccagaccccagaatacagctgctgaaaaccaaagatataagcttgcag
caagatgtgtacccctcctccaacactgtgaattatgagatatgtgacatgagatatggg
tag

>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_4|376_aa
MQLEHQQKMLGLSDSGTNYASTAAPEKGPRPKEQPYCGTFSSHGRQQKIKRDEPKYALSH
NLVLRTGTLSLPPMFHGSKQQLRPSPTSVEQGCFGEQTCQRSLPTANISQHQPLDMVMTS
NSIRREERVVMKHAPLVLLDSEEPHMEPAGHRAVWFAKFQPQNHRVEYRQLRTPASAGIP
EDRFSVIVNHTTWSSCHSRQQTADSLTSKHRGLVMGSHKKWQSKSTARVVGILLETAGTC
SRWNRKASAAFQCQPVSERDRFVGFPAMLTPESLDHIPQSQDFQLCRPGTAKCQPAQWRV
RVVAPALLAPEFSFGVQEESAHTNRLKVGKKYQEWGIAEKIPENVEVTLEPGNRQRLEQF
EGLRRRQGKVEKFGTS

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_4|1131_bp
atgcagcttgagcatcagcagaagatgctgggtctttctgactccggaaccaactatgcc
tctactgctgcccctgaaaagggtcccaggcctaaagagcaaccatactgtgggacattc
tcttctcatggcagacagcagaagatcaagagggacgaaccaaaatatgccttgtctcat
aaccttgtgcttagaactggcactttgtcgcttccacccatgttccacggatcaaagcaa
caattaaggccaagccccacatcagtggagcaggggtgtttcggggaacagacctgtcag
aggtccttgccaacagccaacatcagtcagcatcaaccactagacatggtgatgacatca
aactcaattcggagagaggaaagggtagttatgaagcatgctccactggtgctgctggat
agtgaggagccccacatggagccagctggccacagagcagtgtggttcgccaagttccag
ccccagaatcacagggtagagtacagacagttgaggacaccagcctctgcagggatccct
gaagacagattctcagtcattgtcaaccacaccacctggtccagctgtcacagccgccag
cagacagccgactccctcaccagtaaacaccgcggcttagtgatgggcagccacaagaag
tggcagagcaagagcacagccagagttgtcgggatcctgctggaaacagcaggcacatgc
tcccgttggaacaggaaggcatcagctgcttttcagtgccagccagtgagtgaaagggat
aggtttgtgggattccctgcgatgctgacacctgagagcctggaccacatcccacagtcc
caggattttcagctctgccgtccaggtacggccaagtgccaaccagctcagtggagagtc
agggtggtagcccctgccctcttggcacctgagttctcgtttggcgtccaggaagaatca
gctcacacgaaccgtttgaaagtgggtaagaaataccaggagtggggtattgctgaaaag
atacctgaaaatgtggaagtgactttggaaccgggtaacaggcagagattggaacagttt
gaagggctcagaagaagacaaggaaaagtggaaaagtttggaacttcctag

>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_5|144_aa
MAAAAAAGSGTPREEEGPAGEAAASQPQAPTSVPGARLSRLPLARVKALVKADPDVTLAG
QEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEAWMQLLL
LRAEMKKVFCISGFLNDEDQNKGF

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_5|435_bp
atggcggcggcggcggcggcaggaagcgggacgccccgagaggaggagggacctgctggg
gaggcagcggcctcgcagccccaggccccaacgagtgtgcctggggctcgtctctcgagg
ttgcctctggcgcgagtgaaggccttggtgaaggcagatcccgacgtgacgctagcggga
caggaagccatcttcattctggcacgagccgcggaactgtttgtggagaccattgcaaaa
gatgcctactgttgcgctcagcagggaaaaaggaaaacccttcagaggagagacttggat
aatgcaatagaagctgtggatgaatttgcttttctggaagcctggatgcagctgctgctg
cttagagcagagatgaagaaagtgttctgcataagtggcttcctgaatgatgaggaccag
aataaaggtttttga

>gi568815596f:74734581_74990938|GENSCAN_predicted_peptide_6|761_aa
MKRELRIKNLTQNRSTTWKLNNLLLNDYWVHNEMKAEIKMFFETNENKDTTYQNLWDIFK
AVCRGKFIALNAHKRKQERSKTDTLTSQLKELEKQEQTHSKASRRQEITKIRAELKEIET
QKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDAIKNDKGDITTDPTEIQTTIR
EYYKHLYANKLEDLEEMDKFLNTYTLPRLNQEEVESLNRPITGAEIVAIINSLPTKKSPG
PDGFTAEFNQRYKEELVPFLLKLFQSIEKEGILPNSLDEASIILIPKPGRDTTKKENFRP
ISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHITRTKDK
NHMIISIDAEKAFDKIQQPFMLKTLNKLGIDGTYLKIIRAIYDKPTANSILNGQKLEAFP
LKTGTRQGCPLSPLLFNIVLEVLARAMRQEKEIKGIQLGKEEVKLSLFADDMIVYLENPI
VSAQNLLKLISNFSKVSGYKINVQKSQAFLYTNNRQTESQIMSELPFTIASKRIKYLRIQ
LTRDVKDLFKENYKPLVKEIKEDTNKWKNIPCSWVGRISIVKMAIPPKVIYRFNAIPIKL
PMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKAGGITLPDFKLYYKATVTKTAWYWYQ
NRDIDQWNRTEPSEITPHIYNYLIFDKPEKNKQWGKDSLFNKWCWENWLAICRKLKLDPF
LTPYTKINSRWIKDLNIRPKTTKTLEENLGITILHGQGLHV

>gi568815596f:74734581_74990938|GENSCAN_predicted_CDS_6|2286_bp
atgaaacgagaactcaggattaagaatctcactcaaaaccgctcaactacatggaaactg
aacaacctgctcctgaatgactactgggtacataacgaaatgaaggcagaaataaagatg
ttctttgaaaccaacgagaacaaagacacaacataccagaatctctgggacatattcaaa
gcagtgtgtagagggaaatttatagcactaaatgcccacaagagaaagcaggaaagatcc
aaaactgacaccctaacatcacaattaaaagaactagaaaagcaagagcaaacacattca
aaagctagcagaaggcaagaaataactaagatcagagcagaactgaaggaaatagagaca
caaaaaacccttcaaaaaattaacgaatccaggagctggttttttgaaaggatcaacaaa
attgatagaccgctagcaagactaataaagaaaaagagagagaagaatcaaatagacgca
ataaaaaatgataaaggggatatcaccaccgatcccacagaaatacaaactaccatcaga
gaatactacaaacacctctacgcaaataaactagaagatctagaagaaatggataaattc
ctcaacacatacactctcccaagactaaaccaggaagaagttgaatctctgaatagacca
ataacaggagctgaaattgtggcaataatcaatagcttaccaaccaaaaagagtccagga
ccagatggattcacagctgaattcaaccagaggtacaaggaggaactggtaccattcctt
ctgaaactattccaatcaatagaaaaagagggaatcctccctaactcacttgatgaggcc
agcatcatcctgataccaaagccaggcagagacacaaccaaaaaagagaattttagacca
atatccctgatgaacattgatgcaaaaatcctcaataaaatactggcaaaccgaatccag
cagcacatcaaaaagcttatccaccatgatcaagtgggcttcatccctgggatgcaaggc
tggttcaatatacgcaaatcaataaatgtaatccagcatataaccagaaccaaagacaaa
aaccacatgattatctcaatagatgcagaaaaggcctttgacaaaattcaacaacccttc
atgctaaaaactctcaataaattaggtattgatgggacgtatctcaaaatcataagagct
atctatgacaaacccacagccaatagcatactgaatgggcaaaaactggaagcattccct
ttgaaaactggcacaagacagggatgccctctctcaccactcctattcaacatagtgttg
gaagttctggccagggcaatgaggcaggagaaggaaataaagggtattcagctaggaaaa
gaggaagtcaaattgtccctgtttgcagatgacatgattgtatatctagaaaaccccatt
gtctcagcccaaaatctccttaagctgataagcaacttcagcaaagtctcaggatacaaa
atcaatgtacaaaaatcacaagcattcttatacaccaacaacagacaaacagagagccaa
atcatgagtgaactcccattcacaattgcttcaaagagaataaaatacctaagaatccaa
cttacaagggatgtgaaagacctcttcaaggagaactacaaaccactggtcaaggaaata
aaagaggatacaaacaaatggaagaacattccatgctcatgggtaggaagaatcagtatt
gtgaaaatggccataccgcccaaggtaatttacagattcaatgccatccccatcaagcta
ccaatgactttctttacagaattggaaaaaactactttaaagttcatatggaaccaaaaa
agagcccgcatcgccaagtcaatcctaagccaaaagaacaaagctggaggcatcacgcta
cctgacttcaaactatactataaggctacagtaaccaaaacagcatggtactggtaccaa
aacagagatatagatcaatggaacagaacagagccctcagaaataacgccgcatatctac
aactatctgatctttgacaaacctgagaaaaacaagcaatggggaaaggattccctattt
aataaatggtgctgggaaaactggctagccatatgtagaaagctgaaactggatcccttc
cttacaccttatacaaaaatcaattcaagatggattaaagacttaaacattagacctaaa
accacaaaaaccctagaagaaaacctaggcattaccattctgcatgggcaaggacttcat
gtctaa