Restriction map for pF1KB3021
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BcgI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstXI       CelII       Cfr10I      
ClaI        CpoI        DrdI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
KasI        KpnI        MroI        MslI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1241  GTATAC               GT^[AC][GT]AC
Alw44I        779  GTGCAC               G^TGCAC
AocI           85  CCTGAGG              CC^T[ACGT]AGG
ApaLI         779  GTGCAC               G^TGCAC
Asp700I      1999  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1484  TGGCCA               TGG^CCA
BanI           63  GGCACC               G^G[CT][GA]CC
BbsI         2275  GTCTTC               GAAGAC(2/6)
BbvCI        1898  CCTCAGC              CCTCAGC(-5/-2)
BpuAI        2275  GTCTTC               GAAGAC(2/6)
BsaBI        1810  GATATGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1218  CTGCAC               GTGCAG(16/14)
BsmI         2729  GAATGC               GAATGC(1/-1)
BspLU11I      502  ACATGT               A^CATGT
BspMI         122  ACCTGC               ACCTGC(4/8)
Bst1107I     1241  GTATAC               GTA^TAC
BstAPI       2055  GCACACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        252  GGATG                GGATG(2/0)
BstZ17I      1241  GTATAC               GTA^TAC
Bsu36I         85  CCTGAGG              CC^T[ACGT]AGG
CfoI         1466  GCGC                 GCG^C
DraIII       1991  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
Eco81I         85  CCTGAGG              CC^T[ACGT]AGG
EcoRI         900  GAATTC               G^AATTC
EcoRV         540  GATATC               GAT^ATC
EcoT14I      1543  CCATGG               C^C[AT][AT]GG
FokI          252  GGATG                GGATG(9/13)
HhaI         1466  GCGC                 GCG^C
HinP1I       1466  GCGC                 G^CGC
HpaI          225  GTTAAC               GTT^AAC
KspI           50  CCGCGG               CCGC^GG
MaeII        2220  ACGT                 A^CGT
MamI         1810  GATATGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2501  CAATTG               C^AATTG
MluI         1858  ACGCGT               A^CGCGT
MluNI        1484  TGGCCA               TGG^CCA
MscI         1484  TGGCCA               TGG^CCA
MunI         2501  CAATTG               C^AATTG
NcoI         1543  CCATGG               C^CATGG
NheI         1968  GCTAGC               G^CTAGC
NspI          502  ACATGT               [GA]CATG^[CT]
PvuII         722  CAGCTG               CAG^CTG
SacII          50  CCGCGG               CCGC^GG
SfaNI         994  GATGC                GCATC(5/9)
SspI         2299  AATATT               AAT^ATT
StyI         1543  CCATGG               C^C[AT][AT]GG
Tsp45I       2337  GTCAC                ^GT[GC]AC
XbaI          990  TCTAGA               T^CTAGA
XmnI         1999  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII          51  CGCG                 CG^CG
             1859  CGCG                
AfaI          422  GTAC                 GT^AC
             1502  GTAC                
AlwNI        2284  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2695  CAGAAACTG           
AseI          736  ATTAAT               AT^TAAT
             1152  ATTAAT              
AsnI          736  ATTAAT               AT^TAAT
             1152  ATTAAT              
BciVI        1267  GGATAC               GTATCC(6/5)
             2760  GGATAC              
BcnI           60  CCCGG                CC^[GC]GG
              114  CCCGG               
BglII         987  AGATCT               A^GATCT
             1831  AGATCT              
BseRI          88  GAGGAG               GAGGAG(10/8)
             2239  GAGGAG              
BsiEI          26  CGGCCG               CG[GA][CT]^CG
             2565  CGACCG              
BsmFI        1302  GGGAC                GGGAC(10/14)
             2518  GGGAC               
BspHI        1014  TCATGA               T^CATGA
             2081  TCATGA              
BstUI          51  CGCG                 CG^CG
             1859  CGCG                
Cac8I        1462  GCGTGC               GC[ACGT]^[ACGT]GC
             1968  GCTAGC              
DsaI           50  CCGCGG               C^C[GA][CT]GG
             1543  CCATGG              
EaeI           26  CGGCCG               [CT]^GGCC[GA]
             1484  TGGCCA              
HincII        225  GTTAAC               GT[CT]^[GA]AC
              322  GTTGAC              
HindII        225  GTTAAC               GT[CT]^[GA]AC
              322  GTTGAC              
MvnI           51  CGCG                 CG^CG
             1859  CGCG                
NciI           60  CCCGG                CC^[GC]GG
              114  CCCGG               
NdeI         1447  CATATG               CA^TATG
             2130  CATATG              
PpuMI          92  AGGACCC              [GA]G^G[AT]CC[CT]
              119  AGGACCT             
PshBI         736  ATTAAT               AT^TAAT
             1152  ATTAAT              
PstI         1802  CTGCAG               CTGCA^G
             2062  CTGCAG              
RcaI         1014  TCATGA               T^CATGA
             2081  TCATGA              
RsaI          422  GTAC                 GT^AC
             1502  GTAC                
SmlI          139  CTTGAG               C^T[CT][GA]AG
             1471  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AflIII      AluI        AlwI        
ApoI        AspHI       AvaII       BbvI        BclI        
BfaI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstNI       BstYI       Cfr13I      DdeI        
DpnII       DraI        DraII       EarI        EcoO109I    
EcoRII      FbaI        Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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