Restriction map for pF1KB3021
 (Linear) (Six-base) MAPSORT of: KIBB3021.seq  Check: 2497  from: 1  to: 2784



KIBB3021.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0    1242    2784 
   Size:       1242    1542
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     468    1012    1172    2446    2609    2784 
   Size:        468     544     160    1274     163     175

  Fragments arranged by size: 

                1274     544     468     175     163     160
 
AflIII A'CryG_T

Cuts at:      0     502    1858    2220    2784 
   Size:        502    1356     362     564

  Fragments arranged by size: 

                1356     564     502     362
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2289    2700    2784 
   Size:       2289     411      84

  Fragments arranged by size: 

                2289     411      84
 
ApaLI G'TGCA_C

Cuts at:      0     779    2784 
   Size:        779    2005
 
ApoI r'AATT_y

Cuts at:      0     388     555     882     900     968    1167    1285    1413 
   Size:        388     167     327      18      68     199     118     128

Cuts at:   1413    1866    2158    2441    2686    2784 
   Size:        453     292     283     245      98

  Fragments arranged by size: 

                 453     388     327     292     283     245     199     167
                 128     118      98      68      18
 
AseI AT'TA_AT

Cuts at:      0     737    1153    2784 
   Size:        737     416    1631

  Fragments arranged by size: 

                1631     737     416
 
BanI G'GyrC_C

Cuts at:      0      63    2784 
   Size:         63    2721
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2268    2784 
   Size:       2268     516
 
BbvCI CC'TCA_GC

Cuts at:      0    1899    2784 
   Size:       1899     885
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1261    2754    2784 
   Size:       1261    1493      30

  Fragments arranged by size: 

                1493    1261      30
 
BclI T'GATC_A

Cuts at:      0    1179    1749    2228    2784 
   Size:       1179     570     479     556

  Fragments arranged by size: 

                1179     570     556     479
 
BglII A'GATC_T

Cuts at:      0     987    1831    2784 
   Size:        987     844     953

  Fragments arranged by size: 

                 987     953     844
 
Bme1580I G_kGCm'C

Cuts at:      0     783    2784 
   Size:        783    2001
 
BmtI G_CTAG'C

Cuts at:      0    1972    2784 
   Size:       1972     812
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1103    1771    2420    2784 
   Size:       1103     668     649     364

  Fragments arranged by size: 

                1103     668     649     364
 
Bpu10I CC'TnA_GC

Cuts at:      0    1899    2784 
   Size:       1899     885
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160    1492    2784 
   Size:        160    1332    1292

  Fragments arranged by size: 

                1332    1292     160
 
BsaBI GATnn'nnATC

Cuts at:      0    1814    2784 
   Size:       1814     970
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103    2254    2784 
   Size:        103    2151     530

  Fragments arranged by size: 

                2151     530     103
 
BseYI C'CCAG_C

Cuts at:      0     529    2784 
   Size:        529    2255
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1203    2784 
   Size:       1203    1581
 
BsiEI CG_ry'CG

Cuts at:      0      29    2568    2784 
   Size:         29    2539     216

  Fragments arranged by size: 

                2539     216      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     783    2018    2576    2784 
   Size:        783    1235     558     208

  Fragments arranged by size: 

                1235     783     558     208
 
BsmI GAATG_Cn'

Cuts at:      0    2735    2784 
   Size:       2735      49
 
Bsp1286I G_dGCh'C

Cuts at:      0     783    2018    2576    2784 
   Size:        783    1235     558     208

  Fragments arranged by size: 

                1235     783     558     208
 
BspHI T'CATG_A

Cuts at:      0    1014    2081    2784 
   Size:       1014    1067     703

  Fragments arranged by size: 

                1067    1014     703
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     131    2784 
   Size:        131    2653
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2061    2784 
   Size:       2061     723
 
BstYI r'GATC_y

Cuts at:      0     987    1831    2187    2784 
   Size:        987     844     356     597

  Fragments arranged by size: 

                 987     844     597     356
 
BstZ17I GTA'TAC

Cuts at:      0    1243    2784 
   Size:       1243    1541
 
Bsu36I CC'TnA_GG

Cuts at:      0      86    2784 
   Size:         86    2698
 
BtgI C'CryG_G

Cuts at:      0      50    1543    2784 
   Size:         50    1493    1241

  Fragments arranged by size: 

                1493    1241      50
 
BtsI GCAGTG_nn'

Cuts at:      0    1799    2784 
   Size:       1799     985
 
DraI TTT'AAA

Cuts at:      0    1166    1278    1568    2784 
   Size:       1166     112     290    1216

  Fragments arranged by size: 

                1216    1166     290     112
 
DraIII CAC_nnn'GTG

Cuts at:      0    1996    2784 
   Size:       1996     788
 
EaeI y'GGCC_r

Cuts at:      0      26    1484    2784 
   Size:         26    1458    1300

  Fragments arranged by size: 

                1458    1300      26
 
EagI C'GGCC_G

Cuts at:      0      26    2784 
   Size:         26    2758
 
EarI CTCTTCn'nnn_

Cuts at:      0     487    1031    1085    2231    2422    2771    2784 
   Size:        487     544      54    1146     191     349      13

  Fragments arranged by size: 

                1146     544     487     349     191      54      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     468    1012    1103    1172    1771    2420    2446    2609 
   Size:        468     544      91      69     599     649      26     163

Cuts at:   2609    2784 
   Size:        175

  Fragments arranged by size: 

                 649     599     544     468     175     163      91      69
                  26
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     120    2324    2784 
   Size:         93      27    2204     460

  Fragments arranged by size: 

                2204     460      93      27
 
EcoRI G'AATT_C

Cuts at:      0     900    2784 
   Size:        900    1884
 
EcoRV GAT'ATC

Cuts at:      0     542    2784 
   Size:        542    2242
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     186     218     322     354    2393    2425    2474    2506 
   Size:        186      32     104      32    2039      32      49      32

Cuts at:   2506    2703    2735    2784 
   Size:        197      32      49

  Fragments arranged by size: 

                2039     197     186     104      49      49      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     227     324    2784 
   Size:        227      97    2460

  Fragments arranged by size: 

                2460     227      97
 
HpaI GTT'AAC

Cuts at:      0     227    2784 
   Size:        227    2557
 
MfeI C'AATT_G

Cuts at:      0    2501    2784 
   Size:       2501     283
 
MluI A'CGCG_T

Cuts at:      0    1858    2784 
   Size:       1858     926
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1170    2784 
   Size:       1170    1614
 
MscI TGG'CCA

Cuts at:      0    1486    2784 
   Size:       1486    1298
 
MspA1I CmG'CkG

Cuts at:      0      43      52     110     724    2784 
   Size:         43       9      58     614    2060

  Fragments arranged by size: 

                2060     614      58      43       9
 
NcoI C'CATG_G

Cuts at:      0    1543    2784 
   Size:       1543    1241
 
NdeI CA'TA_TG

Cuts at:      0    1448    2131    2784 
   Size:       1448     683     653

  Fragments arranged by size: 

                1448     683     653
 
NheI G'CTAG_C

Cuts at:      0    1968    2784 
   Size:       1968     816
 
NspI r_CATG'y

Cuts at:      0     506    2784 
   Size:        506    2278
 
PciI A'CATG_T

Cuts at:      0     502    2784 
   Size:        502    2282
 
PfoI T'CCnGG_A

Cuts at:      0    2397    2784 
   Size:       2397     387
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357    2784 
   Size:        325      32    2427

  Fragments arranged by size: 

                2427     325      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     120    2784 
   Size:         93      27    2664

  Fragments arranged by size: 

                2664      93      27
 
PsiI TTA'TAA

Cuts at:      0    1952    2784 
   Size:       1952     832
 
PstI C_TGCA'G

Cuts at:      0    1806    2066    2784 
   Size:       1806     260     718

  Fragments arranged by size: 

                1806     718     260
 
PvuII CAG'CTG

Cuts at:      0     724    2784 
   Size:        724    2060
 
SacII CC_GC'GG

Cuts at:      0      53    2784 
   Size:         53    2731
 
SfcI C'TryA_G

Cuts at:      0     358    1040    1802    2062    2784 
   Size:        358     682     762     260     722

  Fragments arranged by size: 

                 762     722     682     358     260
 
SmlI C'TyrA_G

Cuts at:      0     139    1471    2784 
   Size:        139    1332    1313

  Fragments arranged by size: 

                1332    1313     139
 
SspI AAT'ATT

Cuts at:      0    2301    2784 
   Size:       2301     483
 
StyI C'CwwG_G

Cuts at:      0    1543    2784 
   Size:       1543    1241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     771    1748    2033    2262    2784 
   Size:        771     977     285     229     522

  Fragments arranged by size: 

                 977     771     522     285     229
 
TatI w'GTAC_w

Cuts at:      0    1501    2784 
   Size:       1501    1283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1212    1244    2784 
   Size:       1212      32    1540

  Fragments arranged by size: 

                1540    1212      32
 
XbaI T'CTAG_A

Cuts at:      0     990    2784 
   Size:        990    1794
 
XmnI GAAnn'nnTTC

Cuts at:      0    2003    2784 
   Size:       2003     781
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI    ApaLI     ApoI     AseI     BanI
     BbsI    BbvCI    BciVI     BclI    BglII Bme1580I     BmtI     BpmI
   Bpu10I    BpuEI    BsaBI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI   BstAPI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     DraI   DraIII     EaeI     EagI     EarI  Eco57MI
 EcoO109I    EcoRI    EcoRV    Hin4I   HincII     HpaI     MfeI     MluI
     MmeI     MscI   MspA1I     NcoI     NdeI     NheI     NspI     PciI
     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII    SacII     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BcgI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BtgZI     ClaI    CspCI     DrdI
     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MslI     NaeI     NarI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I
     XcmI     XhoI     XmaI     ZraI
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