Restriction map for
pF1KB3003
(Circular) (Six-base) MAPSORT of: pF1KB3003.seq Check: 8058 from: 1 to: 5012
pF1KB3003 5012 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2050 2050
Size: 5012
Acc65I G'GTAC_C
Cuts at: 1307 2028 1307
Size: 721 4291
AclI AA'CG_TT
Cuts at: 4852 4852
Size: 5012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1051 1490 2837 3269 3622 4284 666
Size: 385 439 1347 432 353 662 1394
Fragments arranged by size:
1394 1347 662 439 432 385 353
AfeI AGC'GCT
Cuts at: 478 478
Size: 5012
AflIII A'CryG_T
Cuts at: 2364 4164 4445 2364
Size: 1800 281 2931
Fragments arranged by size:
2931 1800 281
AleI CACnn'nnGTG
Cuts at: 1361 1361
Size: 5012
AlwNI CAG_nnn'CTG
Cuts at: 970 1717 2317 3755 970
Size: 747 600 1438 2227
Fragments arranged by size:
2227 1438 747 600
ApaLI G'TGCA_C
Cuts at: 3850 3850
Size: 5012
ApoI r'AATT_y
Cuts at: 2016 4365 4621 2016
Size: 2349 256 2407
Fragments arranged by size:
2407 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5012
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
AvaI C'yCGr_G
Cuts at: 974 1004 1034 1169 1825 2032 974
Size: 30 30 135 656 207 3954
Fragments arranged by size:
3954 656 207 135 30 30
AvrII C'CTAG_G
Cuts at: 1227 1227
Size: 5012
BamHI G'GATC_C
Cuts at: 1878 2037 1878
Size: 159 4853
BanI G'GyrC_C
Cuts at: 1015 1307 1995 2028 2442 2690 2725 1015
Size: 292 688 33 414 248 35 3302
Fragments arranged by size:
3302 688 414 292 248 35 33
BanII G_rGCy'C
Cuts at: 675 1025 1724 1826 2026 3056 675
Size: 350 699 102 200 1030 2631
Fragments arranged by size:
2631 1030 699 350 200 102
BbeI G_GCGC'C
Cuts at: 2446 2694 2446
Size: 248 4764
BbsI GAAGACnn'nnnn_
Cuts at: 235 880 1649 235
Size: 645 769 3598
Fragments arranged by size:
3598 769 645
BbvCI CC'TCA_GC
Cuts at: 777 1582 777
Size: 805 4207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4978
BciVI GTATCCnnnnn_n'
Cuts at: 2910 3966 5012 2910
Size: 1056 1046 2910
Fragments arranged by size:
2910 1056 1046
BclI T'GATC_A
Cuts at: 1859 2532 1859
Size: 673 4339
BglI GCCn_nnn'nGGC
Cuts at: 410 1238 410
Size: 828 4184
BglII A'GATC_T
Cuts at: 2527 4564 2527
Size: 2037 2975
BlpI GC'TnA_GC
Cuts at: 300 2124 300
Size: 1824 3188
Bme1580I G_kGCm'C
Cuts at: 2637 2730 3854 2637
Size: 93 1124 3795
Fragments arranged by size:
3795 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 700 958 2224 2305 2640 4784 700
Size: 258 1266 81 335 2144 928
Fragments arranged by size:
2144 1266 928 335 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 739 770 777 1483 356
Size: 383 31 7 706 3885
Fragments arranged by size:
3885 706 383 31 7
Bpu10I CC'TnA_GC
Cuts at: 777 1582 1718 777
Size: 805 136 4071
Fragments arranged by size:
4071 805 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 558 2188 3067 3781 4079 166
Size: 392 1630 879 714 298 1099
Fragments arranged by size:
1630 1099 879 714 392 298
BsaI GGTCTCn'nnnn_
Cuts at: 915 1468 4716 915
Size: 553 3248 1211
Fragments arranged by size:
3248 1211 553
BsaAI yAC'GTr
Cuts at: 2365 2365
Size: 5012
BsaBI GATnn'nnATC
Cuts at: 6 2526 4563 6
Size: 2520 2037 455
Fragments arranged by size:
2520 2037 455
BsaHI Gr'CG_yC
Cuts at: 2443 2691 3393 4889 2443
Size: 248 702 1496 2566
Fragments arranged by size:
2566 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2201 2722 3400 3811 3958 4232 2201
Size: 521 678 411 147 274 2981
Fragments arranged by size:
2981 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1481 1511 1897 1927 1481
Size: 30 386 30 4566
Fragments arranged by size:
4566 386 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 652 765 1022 1063 1354 1599 1645 1906
Size: 375 113 257 41 291 245 46 261
Cuts at: 1906 277
Size: 3383
Fragments arranged by size:
3383 375 291 261 257 245 113 46
41
BseYI C'CCAG_C
Cuts at: 343 479 723 880 896 951 1053 1441 1882
Size: 136 244 157 16 55 102 388 441
Cuts at: 1882 2437 3195 3860 4268 343
Size: 555 758 665 408 1087
Fragments arranged by size:
1087 758 665 555 441 408 388 244
157 136 102 55 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 188
Size: 5012
BsiEI CG_ry'CG
Cuts at: 104 2554 2600 3830 104
Size: 2450 46 1230 1286
Fragments arranged by size:
2450 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 981 1499 1724 1826 2026 2804 2994 3854 981
Size: 518 225 102 200 778 190 860 2139
Fragments arranged by size:
2139 860 778 518 225 200 190 102
BsmI GAATG_Cn'
Cuts at: 1921 1921
Size: 5012
Bsp1286I G_dGCh'C
Cuts at: 675 981 1025 1499 1724 1826 2026 2637 2730
Size: 306 44 474 225 102 200 611 93
Cuts at: 2730 2804 2994 3056 3854 675
Size: 74 190 62 798 1833
Fragments arranged by size:
1833 798 611 474 306 225 200 190
102 93 74 62 44
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2276 2578 2959 2062
Size: 214 302 381 4115
Fragments arranged by size:
4115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1112 1782 3304 3358 4842 5007 50
Size: 1062 670 1522 54 1484 165 55
Fragments arranged by size:
1522 1484 1062 670 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1959 2924 1959
Size: 965 4047
BsrFI r'CCGG_y
Cuts at: 3010 3191 3010
Size: 181 4831
BssSI C'ACGA_G
Cuts at: 3283 3991 3283
Size: 708 4304
BstAPI GCAn_nnn'nTGC
Cuts at: 2389 2389
Size: 5012
BstBI TT'CG_AA
Cuts at: 3373 3373
Size: 5012
BstEII G'GTnAC_C
Cuts at: 372 372
Size: 5012
BstXI CCAn_nnnn'nTGG
Cuts at: 240 1987 240
Size: 1747 3265
BstYI r'GATC_y
Cuts at: 1 116 1061 1878 1940 2037 2504 2527 2862
Size: 115 945 817 62 97 467 23 335
Cuts at: 2862 3108 3414 3426 3512 4564 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 945 817 467 449 335 306 246
115 97 86 62 23 12
BtgI C'CryG_G
Cuts at: 1661 1661
Size: 5012
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2962 3143 3306 2962
Size: 181 163 4668
Fragments arranged by size:
4668 181 163
BtsI GCAGTG_nn'
Cuts at: 414 995 1341 2255 414
Size: 581 346 914 3171
Fragments arranged by size:
3171 914 581 346
DraI TTT'AAA
Cuts at: 2011 2011
Size: 5012
DraIII CAC_nnn'GTG
Cuts at: 279 279
Size: 5012
DrdI GACnn_nn'nnGTC
Cuts at: 361 454 2718 4062 361
Size: 93 2264 1344 1311
Fragments arranged by size:
2264 1344 1311 93
EaeI y'GGCC_r
Cuts at: 618 1156 1623 2597 2771 3162 3189 4534 618
Size: 538 467 974 174 391 27 1345 1096
Fragments arranged by size:
1345 1096 974 538 467 391 174 27
EagI C'GGCC_G
Cuts at: 2597 2597
Size: 5012
EarI CTCTTCn'nnn_
Cuts at: 682 835 1466 3035 3245 682
Size: 153 631 1569 210 2449
Fragments arranged by size:
2449 1569 631 210 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 109 115 1567 1800 3220 3962 4108 4822 109
Size: 6 1452 233 1420 742 146 714 299
Fragments arranged by size:
1452 1420 742 714 299 233 146 6
EcoICRI GAG'CTC
Cuts at: 1722 1824 2024 1722
Size: 102 200 4710
Fragments arranged by size:
4710 200 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 666 739 770 777 1051 1483 1490 2837
Size: 310 73 31 7 274 432 7 1347
Cuts at: 2837 3269 3622 4284 356
Size: 432 353 662 1084
Fragments arranged by size:
1347 1084 662 432 432 353 310 274
73 31 7 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1302 1302
Size: 5012
EcoO109I rG'GnC_Cy
Cuts at: 363 585 730 1131 1224 1896 2151 363
Size: 222 145 401 93 672 255 3224
Fragments arranged by size:
3224 672 401 255 222 145 93
EcoRI G'AATT_C
Cuts at: 2016 2016
Size: 5012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1767 3031 1767
Size: 1264 3748
FspI TGC'GCA
Cuts at: 2793 2793
Size: 5012
HaeII r_GCGC'y
Cuts at: 344 480 653 2446 2694 3924 4695 344
Size: 136 173 1793 248 1230 771 661
Fragments arranged by size:
1793 1230 771 661 248 173 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1229 1261 1336 1368 1897 1929 2854
Size: 32 623 32 75 32 529 32 925
Cuts at: 2854 2884 2886 2916 574
Size: 30 2 30 2670
Fragments arranged by size:
2670 925 623 529 75 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2051 2051
Size: 5012
HindIII A'AGCT_T
Cuts at: 613 1387 613
Size: 774 4238
KasI G'GCGC_C
Cuts at: 2442 2690 2442
Size: 248 4764
KpnI G_GTAC'C
Cuts at: 1311 2032 1311
Size: 721 4291
MluI A'CGCG_T
Cuts at: 4445 4445
Size: 5012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 830 964 3771 3955 233
Size: 597 134 2807 184 1290
Fragments arranged by size:
2807 1290 597 184 134
MscI TGG'CCA
Cuts at: 620 1625 2773 620
Size: 1005 1148 2859
Fragments arranged by size:
2859 1148 1005
MslI CAynn'nnrTG
Cuts at: 238 1361 1872 3001 3128 238
Size: 1123 511 1129 127 2122
Fragments arranged by size:
2122 1129 1123 511 127
MspA1I CmG'CkG
Cuts at: 334 745 900 1361 1370 1435 2123 2437 2797
Size: 411 155 461 9 65 688 314 360
Cuts at: 2797 3581 3826 334
Size: 784 245 1520
Fragments arranged by size:
1520 784 688 461 411 360 314 245
155 65 9
NaeI GCC'GGC
Cuts at: 3193 3193
Size: 5012
NarI GG'CG_CC
Cuts at: 2443 2691 2443
Size: 248 4764
NgoMIV G'CCGG_C
Cuts at: 3191 3191
Size: 5012
NspI r_CATG'y
Cuts at: 438 1284 2065 2565 4168 438
Size: 846 781 500 1603 1282
Fragments arranged by size:
1603 1282 846 781 500
PasI CC'CwG_GG
Cuts at: 1771 1771
Size: 5012
PciI A'CATG_T
Cuts at: 4164 4164
Size: 5012
PfoI T'CCnGG_A
Cuts at: 1499 1499
Size: 5012
PmeI GTTT'AAAC
Cuts at: 2011 2011
Size: 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4980
PpuMI rG'GwC_Cy
Cuts at: 363 730 1224 363
Size: 367 494 4151
Fragments arranged by size:
4151 494 367
PspXI vC'TCGA_Gb
Cuts at: 1825 1825
Size: 5012
PstI C_TGCA'G
Cuts at: 145 2059 2744 145
Size: 1914 685 2413
Fragments arranged by size:
2413 1914 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5012
PvuII CAG'CTG
Cuts at: 334 745 900 1435 2437 2797 334
Size: 411 155 535 1002 360 2549
Fragments arranged by size:
2549 1002 535 411 360 155
RsrII CG'GwC_CG
Cuts at: 3207 3207
Size: 5012
SacI G_AGCT'C
Cuts at: 1724 1826 2026 1724
Size: 102 200 4710
Fragments arranged by size:
4710 200 102
SalI G'TCGA_C
Cuts at: 2049 2049
Size: 5012
SapI GCTCTTCn'nnn_
Cuts at: 3035 3245 3035
Size: 210 4802
SbfI CC_TGCA'GG
Cuts at: 2059 2059
Size: 5012
ScaI AGT'ACT
Cuts at: 4529 4529
Size: 5012
SfcI C'TryA_G
Cuts at: 33 141 247 513 1944 2055 2740 3708 3899
Size: 108 106 266 1431 111 685 968 191
Cuts at: 3899 33
Size: 1146
Fragments arranged by size:
1431 1146 968 685 266 191 111 108
106
SfiI GGCCn_nnn'nGGCC
Cuts at: 1238 1238
Size: 5012
SfoI GGC'GCC
Cuts at: 2444 2692 2444
Size: 248 4764
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
SmaI CCC'GGG
Cuts at: 1171 2034 1171
Size: 863 4149
SmlI C'TyrA_G
Cuts at: 181 537 1825 2167 3082 3796 4058 181
Size: 356 1288 342 915 714 262 1135
Fragments arranged by size:
1288 1135 915 714 356 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5012
SphI G_CATG'C
Cuts at: 1284 2065 2565 1284
Size: 781 500 3731
Fragments arranged by size:
3731 781 500
StuI AGG'CCT
Cuts at: 1232 1351 1232
Size: 119 4893
StyI C'CwwG_G
Cuts at: 215 1227 2146 2500 215
Size: 1012 919 354 2727
Fragments arranged by size:
2727 1012 919 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1251 2595 2728 3187 3396 1251
Size: 1344 133 459 209 2867
Fragments arranged by size:
2867 1344 459 209 133
TatI w'GTAC_w
Cuts at: 1214 4527 1214
Size: 3313 1699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1481 1513 4529 4561 1481
Size: 32 3016 32 1932
Fragments arranged by size:
3016 1932 32 32
Tth111I GACn'n_nGTC
Cuts at: 487 2809 487
Size: 2322 2690
XbaI T'CTAG_A
Cuts at: 2043 2043
Size: 5012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1633 2507 1633
Size: 874 4138
XhoI C'TCGA_G
Cuts at: 1825 1825
Size: 5012
XmaI C'CCGG_G
Cuts at: 1169 2032 1169
Size: 863 4149
XmnI GAAnn'nnTTC
Cuts at: 176 1105 176
Size: 929 4083
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI ApaI
AscI BaeI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
ZraI