Restriction map for
pF1KB2497
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbrPI BbvCI BclI BlnI
BsaAI BsaBI BseAI BsgI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I ClaI DraI DraIII
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI SapI SexAI SfiI
SnaBI SpeI SspI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 1413 CTTAAG C^TTAAG
AhdI 1981 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 868 GTGCAC G^TGCAC
ApaLI 868 GTGCAC G^TGCAC
AspEI 1981 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1151 GCAGCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1413 CTTAAG C^TTAAG
BglII 2373 AGATCT A^GATCT
Bsp1407I 1043 TGTACA T^GTACA
BsrDI 1862 CATTGC GCAATG(2/0)
BsrGI 1043 TGTACA T^GTACA
BssSI 1485 CACGAG CACGAG(-5/-1)
BstBI 1159 TTCGAA TT^CGAA
BstXI 574 CCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 777 CGGACCG CG^G[AT]CCG
Eam1105I 1981 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 2352 CCATGG C^C[AT][AT]GG
NcoI 2352 CCATGG C^CATGG
NheI 696 GCTAGC G^CTAGC
NspV 1159 TTCGAA TT^CGAA
PvuII 859 CAGCTG CAG^CTG
RsrII 777 CGGACCG CG^G[AT]CCG
SbfI 1614 CCTGCAGG CCTGCA^GG
ScaI 1849 AGTACT AGT^ACT
SfuI 1159 TTCGAA TT^CGAA
SgrAI 1344 CACCGGCG C[GA]^CCGG[CT]G
SphI 648 GCATGC GCATG^C
Sse8387I 1614 CCTGCAGG CCTGCA^GG
SspBI 1043 TGTACA T^GTACA
StuI 1171 AGGCCT AGG^CCT
StyI 2352 CCATGG C^C[AT][AT]GG
XcmI 571 CCACCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcsI 1325 AAATTC [GA]^AATT[CT]
1755 AAATTC
AlwI 1728 GGATC GGATC(4/5)
2119 GGATC
AocI 733 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
ApoI 1325 AAATTC [GA]^AATT[CT]
1755 AAATTC
Asp700I 1083 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1476 GAAATCTTTC
BbeI 222 GGCGCC GGCGC^C
729 GGCGCC
BbsI 1203 GAAGAC GAAGAC(2/6)
1692 GAAGAC
BciVI 2025 GGATAC GTATCC(6/5)
59 GTATCC
BglI 162 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
184 GCCAGCACGGC
BlpI 700 GCTGAGC GC^T[ACGT]AGC
1738 GCTCAGC
BpmI 379 CTCCAG CTGGAG(16/14)
2148 CTCCAG
Bpu1102I 700 GCTGAGC GC^T[ACGT]AGC
1738 GCTCAGC
BpuAI 1203 GAAGAC GAAGAC(2/6)
1692 GAAGAC
BsaWI 325 TCCGGT [AT]^CCGG[AT]
1442 ACCGGA
BsmFI 31 GGGAC GGGAC(10/14)
115 GTCCC
BsrBI 596 CCGCTC CCGCTC(-3/-3)
393 GAGCGG
Bsu36I 733 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
CelII 700 GCTGAGC GC^T[ACGT]AGC
1738 GCTCAGC
EarI 1669 CTCTTC CTCTTC(1/4)
1828 CTCTTC
Eco81I 733 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
EcoNI 1459 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1549 CCTGTGGCAGG
KasI 222 GGCGCC G^GCGCC
729 GGCGCC
Ksp632I 1669 CTCTTC CTCTTC(1/4)
1828 CTCTTC
MaeII 308 ACGT A^CGT
2279 ACGT
NaeI 102 GCCGGC GCC^GGC
705 GCCGGC
NarI 222 GGCGCC GG^CGCC
729 GGCGCC
NgoMIV 102 GCCGGC G^CCGGC
705 GCCGGC
NspI 648 GCATGC [GA]CATG^[CT]
1400 GCATGT
PpuMI 113 AGGTCCC [GA]G^G[AT]CC[CT]
521 AGGACCT
SacI 1920 GAGCTC GAGCT^C
2070 GAGCTC
SfoI 222 GGCGCC GGC^GCC
729 GGCGCC
XmnI 1083 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1476 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KspI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI