Restriction map for pF1KB2389
##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       BlpI        Bpu1102I    BsaWI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoT22I     FseI        
FspI        HpaI        KpnI        MfeI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1999  GTAGAC               GT^[AC][GT]AC
AcsI         1960  GAATTT               [GA]^AATT[CT]
AflII         147  CTTAAG               C^TTAAG
Alw44I       1663  GTGCAC               G^TGCAC
ApaLI        1663  GTGCAC               G^TGCAC
ApoI         1960  GAATTT               [GA]^AATT[CT]
AvaI          696  CCCGGG               C^[CT]CG[GA]G
AvrII           6  CCTAGG               C^CTAGG
BamHI         686  GGATCC               G^GATCC
BbeI         3140  GGCGCC               GGCGC^C
BbrPI        2812  CACGTG               CAC^GTG
BbvCI        3115  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1169  CGAGCACGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1502  GTATCC               GTATCC(6/5)
BclI          209  TGATCA               T^GATCA
BfrI          147  CTTAAG               C^TTAAG
BlnI            6  CCTAGG               C^CTAGG
BsaAI        2812  CACGTG               [CT]AC^GT[GA]
BsmBI        2469  CGTCTC               CGTCTC(1/5)
BsoBI         696  CCCGGG               C^[CT]CG[GA]G
BspLU11I      438  ACATGT               A^CATGT
BsrFI        1037  ACCGGC               [GA]^CCGG[CT]
BssSI        3088  CACGAG               CACGAG(-5/-1)
BstEII        514  GGTCACC              G^GT[ACGT]ACC
BstPI         514  GGTCACC              G^GT[ACGT]ACC
Cfr10I       1037  ACCGGC               [GA]^CCGG[CT]
DraIII        241  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       514  GGTCACC              G^GT[ACGT]ACC
EcoRV        1201  GATATC               GAT^ATC
FbaI          209  TGATCA               T^GATCA
HincII        448  GTTGAC               GT[CT]^[GA]AC
HindII        448  GTTGAC               GT[CT]^[GA]AC
KasI         3140  GGCGCC               G^GCGCC
KspI          660  CCGCGG               CCGC^GG
NarI         3140  GGCGCC               GG^CGCC
NcoI         1220  CCATGG               C^CATGG
NdeI         1252  CATATG               CA^TATG
PmaCI        2812  CACGTG               CAC^GTG
PmlI         2812  CACGTG               CAC^GTG
PshAI        2002  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         660  CCGCGG               CCGC^GG
SfiI         1638  GGCCTCCGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3140  GGCGCC               GGC^GCC
SmaI          696  CCCGGG               CCC^GGG
StuI          143  AGGCCT               AGG^CCT
XbaI         2031  TCTAGA               T^CTAGA
XmaCI         696  CCCGGG               C^CCGGG
XmaI          696  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AatII         484  GACGTC               GACGT^C
             1387  GACGTC              
AfaI          564  GTAC                 GT^AC
             2424  GTAC                
Aor51HI       425  AGCGCT               AGC^GCT
             3095  AGCGCT              
ApaI          693  GGGCCC               GGGCC^C
             1334  GGGCCC              
Asp700I      2322  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2674  GAATATATTC          
AspI          249  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2854  GACTCAGTC           
BglI          721  GCCAACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1639  GCCTCCGTGGC         
BglII         155  AGATCT               A^GATCT
             2375  AGATCT              
Eco47III      425  AGCGCT               AGC^GCT
             3095  AGCGCT              
HindIII       359  AAGCTT               A^AGCTT
             2383  AAGCTT              
MluI          623  ACGCGT               A^CGCGT
             2042  ACGCGT              
NspI          438  ACATGT               [GA]CATG^[CT]
              734  ACATGC              
PflFI         249  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2854  GACTCAGTC           
PflMI        2106  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2190  CCATATCCTGG         
RsaI          564  GTAC                 GT^AC
             2424  GTAC                
SacI         1876  GAGCTC               GAGCT^C
             2200  GAGCTC              
SapI         2322  GAAGAGC              GCTCTTC(1/4)
             1930  GCTCTTC             
SfaNI        2493  GATGC                GCATC(5/9)
             2480  GCATC               
TaqI          338  TCGA                 T^CGA
             2333  TCGA                
Tth111I       249  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2854  GACTCAGTC           
TthHB8I       338  TCGA                 T^CGA
             2333  TCGA                
Van91I       2106  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2190  CCATATCCTGG         
XcmI          635  CCATGCCCCTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2627  CCAGGAGACTGCTGG     
XmnI         2322  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2674  GAATATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AflIII      AluI        
AlwI        AlwNI       AocI        AspHI       AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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