Restriction map for pF1KB1487
 (Linear) (Six-base) MAPSORT of: KIBB1487.seq  Check: 403  from: 1  to: 5739



KIBB1487.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1687    1753    1761    2089    5647    5680    5739 
   Size:       1687      66       8     328    3558      33      59

  Fragments arranged by size: 

                3558    1687     328      66      59      33       8
 
AccI GT'mk_AC

Cuts at:      0     310    5739 
   Size:        310    5429
 
AclI AA'CG_TT

Cuts at:      0    5015    5739 
   Size:       5015     724
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     997    1349    1579    1976    2296    2638    2764 
   Size:        422     575     352     230     397     320     342     126

Cuts at:   2764    3041    3124    3160    4304    4873    4910    5119    5125 
   Size:        277      83      36    1144     569      37     209       6

Cuts at:   5125    5384    5739 
   Size:        259     355

  Fragments arranged by size: 

                1144     575     569     422     397     355     352     342
                 320     277     259     230     209     126      83      37
                  36       6
 
AfeI AGC'GCT

Cuts at:      0    2100    5739 
   Size:       2100    3639
 
AflIII A'CryG_T

Cuts at:      0     692    2324    4223    4928    5739 
   Size:        692    1632    1899     705     811

  Fragments arranged by size: 

                1899    1632     811     705     692
 
AleI CACnn'nnGTG

Cuts at:      0    1357    3979    5739 
   Size:       1357    2622    1760

  Fragments arranged by size: 

                2622    1760    1357
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     626    3358    5739 
   Size:        626    2732    2381

  Fragments arranged by size: 

                2732    2381     626
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     731     763    3163    3195    5739 
   Size:        731      32    2400      32    2544

  Fragments arranged by size: 

                2544    2400     731      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     651    1787    3309    4179    5739 
   Size:        651    1136    1522     870    1560

  Fragments arranged by size: 

                1560    1522    1136     870     651
 
ApaI G_GGCC'C

Cuts at:      0    3065    5739 
   Size:       3065    2674
 
ApaLI G'TGCA_C

Cuts at:      0    2254    2833    4744    5739 
   Size:       2254     579    1911     995

  Fragments arranged by size: 

                2254    1911     995     579
 
ApoI r'AATT_y

Cuts at:      0    1243    4420    4876    5739 
   Size:       1243    3177     456     863

  Fragments arranged by size: 

                3177    1243     863     456
 
AvaI C'yCGr_G

Cuts at:      0    4333    5509    5739 
   Size:       4333    1176     230

  Fragments arranged by size: 

                4333    1176     230
 
BamHI G'GATC_C

Cuts at:      0      57    5739 
   Size:         57    5682
 
BanI G'GyrC_C

Cuts at:      0    1351    1735    2152    2910    4792    4867    5739 
   Size:       1351     384     417     758    1882      75     872

  Fragments arranged by size: 

                1882    1351     872     758     417     384      75
 
BanII G_rGCy'C

Cuts at:      0     185    1703    2454    3065    3625    4036    4334    4910 
   Size:        185    1518     751     611     560     411     298     576

Cuts at:   4910    5300    5687    5739 
   Size:        390     387      52

  Fragments arranged by size: 

                1518     751     611     576     560     411     390     387
                 298     185      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1024    2597    2741    3356    5274    5739 
   Size:       1024    1573     144     615    1918     465

  Fragments arranged by size: 

                1918    1573    1024     615     465     144
 
BbvCI CC'TCA_GC

Cuts at:      0     941    5022    5399    5739 
   Size:        941    4081     377     340

  Fragments arranged by size: 

                4081     941     377     340
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     524     558    5739 
   Size:        524      34    5181

  Fragments arranged by size: 

                5181     524      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2053    5491    5739 
   Size:       2053    3438     248

  Fragments arranged by size: 

                3438    2053     248
 
BclI T'GATC_A

Cuts at:      0    4895    5739 
   Size:       4895     844
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1693    2556    3060    5739 
   Size:       1693     863     504    2679

  Fragments arranged by size: 

                2679    1693     863     504
 
BglII A'GATC_T

Cuts at:      0    4106    4703    5060    5739 
   Size:       4106     597     357     679

  Fragments arranged by size: 

                4106     679     597     357
 
BlpI GC'TnA_GC

Cuts at:      0    1697    5739 
   Size:       1697    4042
 
Bme1580I G_kGCm'C

Cuts at:      0     478     615    1628    2074    2258    2837    3065    4748 
   Size:        478     137    1013     446     184     579     228    1683

Cuts at:   4748    4795    5739 
   Size:         47     944

  Fragments arranged by size: 

                1683    1013     944     579     478     446     228     184
                 137      47
 
BmgBI CAC'GTC

Cuts at:      0     681    2325    2604    4130    5739 
   Size:        681    1644     279    1526    1609

  Fragments arranged by size: 

                1644    1609    1526     681     279
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1615    5739 
   Size:       1615    4124
 
BmtI G_CTAG'C

Cuts at:      0    3188    5739 
   Size:       3188    2551
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1139    2042    5739 
   Size:       1139     903    3697

  Fragments arranged by size: 

                3697    1139     903
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     876     883     985    1121    1533    2123    3334    3554 
   Size:        876       7     102     136     412     590    1211     220

Cuts at:   3554    4633    4855    5739 
   Size:       1079     222     884

  Fragments arranged by size: 

                1211    1079     884     876     590     412     222     220
                 136     102       7
 
Bpu10I CC'TnA_GC

Cuts at:      0     941    3040    4556    5022    5399    5739 
   Size:        941    2099    1516     466     377     340

  Fragments arranged by size: 

                2099    1516     941     466     377     340
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     568    2371    3184    3397    4741    5407    5739 
   Size:         48     520    1803     813     213    1344     666     332

  Fragments arranged by size: 

                1803    1344     813     666     520     332     213      48
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442    2687    2995    3091    3127    4853    5739 
   Size:        442    2245     308      96      36    1726     886

  Fragments arranged by size: 

                2245    1726     886     442     308      96      36
 
BsaAI yAC'GTr

Cuts at:      0     695    2075    4213    5739 
   Size:        695    1380    2138    1526

  Fragments arranged by size: 

                2138    1526    1380     695
 
BsaBI GATnn'nnATC

Cuts at:      0    4410    4531    5739 
   Size:       4410     121    1208

  Fragments arranged by size: 

                4410    1208     121
 
BsaWI w'CCGG_w

Cuts at:      0    1005    4346    5201    5739 
   Size:       1005    3341     855     538

  Fragments arranged by size: 

                3341    1005     855     538
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1657    1687    3746    3776    4606    4636    4666    4696 
   Size:       1657      30    2059      30     830      30      30      30

Cuts at:   4696    5739 
   Size:       1043

  Fragments arranged by size: 

                2059    1657    1043     830      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307    1115    1377    2516    2599    2665    4026    4053 
   Size:        307     808     262    1139      83      66    1361      27

Cuts at:   4053    4414    5739 
   Size:        361    1325

  Fragments arranged by size: 

                1361    1325    1139     808     361     307     262      83
                  66      27
 
BseYI C'CCAG_C

Cuts at:      0     498    1849    2156    2754    2843    2952    3291    3375 
   Size:        498    1351     307     598      89     109     339      84

Cuts at:   3375    3945    3999    5739 
   Size:        570      54    1740

  Fragments arranged by size: 

                1740    1351     598     570     498     339     307     109
                  89      84      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1443    1847    1954    2371    5112    5658    5739 
   Size:       1443     404     107     417    2741     546      81

  Fragments arranged by size: 

                2741    1443     546     417     404     107      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     342    1074    1606    1703    2129    2258    2837    3544 
   Size:        342     732     532      97     426     129     579     707

Cuts at:   3544    3625    3848    3911    4036    4748    5687    5739 
   Size:         81     223      63     125     712     939      52

  Fragments arranged by size: 

                 939     732     712     707     579     532     426     342
                 223     129     125      97      81      63      52
 
BsiWI C'GTAC_G

Cuts at:      0    1608    5739 
   Size:       1608    4131
 
BsmI GAATG_Cn'

Cuts at:      0    1234    1764    2410    3015    4845    5050    5739 
   Size:       1234     530     646     605    1830     205     689

  Fragments arranged by size: 

                1830    1234     689     646     605     530     205
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2626    3468    5739 
   Size:       2626     842    2271

  Fragments arranged by size: 

                2626    2271     842
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     342     478     615    1074    1606    1628    1703 
   Size:        185     157     136     137     459     532      22      75

Cuts at:   1703    2074    2129    2258    2454    2837    3065    3544    3625 
   Size:        371      55     129     196     383     228     479      81

Cuts at:   3625    3848    3911    4036    4334    4748    4795    4910    5300 
   Size:        223      63     125     298     414      47     115     390

Cuts at:   5300    5687    5739 
   Size:        387      52

  Fragments arranged by size: 

                 532     479     459     414     390     387     383     371
                 298     228     223     196     185     157     137     136
                 129     125     115      81      75      63      55      52
                  47      22
 
BspHI T'CATG_A

Cuts at:      0    4535    5739 
   Size:       4535    1204
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     561     636    1687    1753    1761    2089    2792    2838 
   Size:        561      75    1051      66       8     328     703      46

Cuts at:   2838    2934    3684    4187    5647    5680    5739 
   Size:         96     750     503    1460      33      59

  Fragments arranged by size: 

                1460    1051     750     703     561     503     328      96
                  75      66      59      46      33       8
 
BsrBI CCG'CTC

Cuts at:      0    5340    5739 
   Size:       5340     399
 
BsrDI GCAATG_nn'

Cuts at:      0    3051    3159    3710    5253    5291    5739 
   Size:       3051     108     551    1543      38     448

  Fragments arranged by size: 

                3051    1543     551     448     108      38
 
BsrFI r'CCGG_y

Cuts at:      0     557    1687    2365    5739 
   Size:        557    1130     678    3374

  Fragments arranged by size: 

                3374    1130     678     557
 
BsrGI T'GTAC_A

Cuts at:      0     689    4086    5739 
   Size:        689    3397    1653

  Fragments arranged by size: 

                3397    1653     689
 
BssHII G'CGCG_C

Cuts at:      0    3898    5739 
   Size:       3898    1841
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     887    3309    5135    5739 
   Size:        887    2422    1826     604

  Fragments arranged by size: 

                2422    1826     887     604
 
BstBI TT'CG_AA

Cuts at:      0     155    5739 
   Size:        155    5584
 
BstEII G'GTnAC_C

Cuts at:      0     172     573     822    1791    2236    5324    5739 
   Size:        172     401     249     969     445    3088     415

  Fragments arranged by size: 

                3088     969     445     415     401     249     172
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     907    3021    4833    5175    5739 
   Size:        907    2114    1812     342     564

  Fragments arranged by size: 

                2114    1812     907     564     342
 
BstYI r'GATC_y

Cuts at:      0      57    1985    2378    2750    3830    4106    4411    4703 
   Size:         57    1928     393     372    1080     276     305     292

Cuts at:   4703    5060    5739 
   Size:        357     679

  Fragments arranged by size: 

                1928    1080     679     393     372     357     305     292
                 276      57
 
BstZ17I GTA'TAC

Cuts at:      0     311    5739 
   Size:        311    5428
 
Bsu36I CC'TnA_GG

Cuts at:      0     417    2545    3485    3731    5739 
   Size:        417    2128     940     246    2008

  Fragments arranged by size: 

                2128    2008     940     417     246
 
BtgI C'CryG_G

Cuts at:      0     660    1211    1731    2458    3074    3146    3557    5149 
   Size:        660     551     520     727     616      72     411    1592

Cuts at:   5149    5739 
   Size:        590

  Fragments arranged by size: 

                1592     727     660     616     590     551     520     411
                  72
 
BtsI GCAGTG_nn'

Cuts at:      0     339     449     598    1460    1606    1859    3000    3026 
   Size:        339     110     149     862     146     253    1141      26

Cuts at:   3026    3478    3909    3925    4086    5140    5739 
   Size:        452     431      16     161    1054     599

  Fragments arranged by size: 

                1141    1054     862     599     452     431     339     253
                 161     149     146     110      26      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     315     350    5739 
   Size:        315      35    5389

  Fragments arranged by size: 

                5389     315      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    4015    5135    5739 
   Size:       4015    1120     604

  Fragments arranged by size: 

                4015    1120     604
 
EaeI y'GGCC_r

Cuts at:      0     555    2295    2367    2557    2870    3528    3893    4823 
   Size:        555    1740      72     190     313     658     365     930

Cuts at:   4823    5009    5739 
   Size:        186     730

  Fragments arranged by size: 

                1740     930     730     658     555     365     313     190
                 186      72
 
EarI CTCTTCn'nnn_

Cuts at:      0    1100    1108    1804    1816    2614    2816    3632    4280 
   Size:       1100       8     696      12     798     202     816     648

Cuts at:   4280    5720    5729    5739 
   Size:       1440       9      10

  Fragments arranged by size: 

                1440    1100     816     798     696     648     202      12
                  10       9       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2650    3550    5739 
   Size:       2650     900    2189

  Fragments arranged by size: 

                2650    2189     900
 
EcoICRI GAG'CTC

Cuts at:      0    1701    3623    4034    5685    5739 
   Size:       1701    1922     411    1651      54

  Fragments arranged by size: 

                1922    1701    1651     411      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     876     883     985     997    1121    1349    1533 
   Size:        422     454       7     102      12     124     228     184

Cuts at:   1533    1579    1976    2123    2296    2638    2764    3041    3124 
   Size:         46     397     147     173     342     126     277      83

Cuts at:   3124    3160    3334    3554    4304    4633    4855    4873    4910 
   Size:         36     174     220     750     329     222      18      37

Cuts at:   4910    5119    5125    5384    5739 
   Size:        209       6     259     355

  Fragments arranged by size: 

                 750     454     422     397     355     342     329     277
                 259     228     222     220     209     184     174     173
                 147     126     124     102      83      46      37      36
                  18      12       7       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      88    1723    3439    5739 
   Size:         88    1635    1716    2300

  Fragments arranged by size: 

                2300    1716    1635      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93    1086    1616    1881    2487    2540    3061    3062 
   Size:         93     993     530     265     606      53     521       1

Cuts at:   3062    3591    4176    4857    5739 
   Size:        529     585     681     882

  Fragments arranged by size: 

                 993     882     681     606     585     530     529     521
                 265      93      53       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4878    5401    5739 
   Size:       4878     523     338

  Fragments arranged by size: 

                4878     523     338
 
FseI GG_CCGG'CC

Cuts at:      0     561    5739 
   Size:        561    5178
 
HaeII r_GCGC'y

Cuts at:      0    1787    2102    5739 
   Size:       1787     315    3637

  Fragments arranged by size: 

                3637    1787     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     529    1387    1419    2845    2877    2929    2961 
   Size:        497      32     858      32    1426      32      52      32

Cuts at:   2961    3589    3621    3774    3806    4606    4638    5047    5079 
   Size:        628      32     153      32     800      32     409      32

Cuts at:   5079    5521    5553    5739 
   Size:        442      32     186

  Fragments arranged by size: 

                1426     858     800     628     497     442     409     186
                 153      52      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      72     362     669    3969    4782    5652    5739 
   Size:         72     290     307    3300     813     870      87

  Fragments arranged by size: 

                3300     870     813     307     290      87      72
 
HindIII A'AGCT_T

Cuts at:      0    5378    5739 
   Size:       5378     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1493    5090    5739 
   Size:       1493    3597     649

  Fragments arranged by size: 

                3597    1493     649
 
MscI TGG'CCA

Cuts at:      0    2297    2559    2872    3530    4825    5011    5739 
   Size:       2297     262     313     658    1295     186     728

  Fragments arranged by size: 

                2297    1295     728     658     313     262     186
 
MslI CAynn'nnrTG

Cuts at:      0    1357    3853    3979    4603    4927    5739 
   Size:       1357    2496     126     624     324     812

  Fragments arranged by size: 

                2496    1357     812     624     324     126
 
MspA1I CmG'CkG

Cuts at:      0     224     736    1457    2480    2682    2790    4003    5739 
   Size:        224     512     721    1023     202     108    1213    1736

  Fragments arranged by size: 

                1736    1213    1023     721     512     224     202     108
            
 
NaeI GCC'GGC

Cuts at:      0     559    1689    2367    5739 
   Size:        559    1130     678    3372

  Fragments arranged by size: 

                3372    1130     678     559
 
NcoI C'CATG_G

Cuts at:      0     660    1211    1731    3146    5149    5739 
   Size:        660     551     520    1415    2003     590

  Fragments arranged by size: 

                2003    1415     660     590     551     520
 
NdeI CA'TA_TG

Cuts at:      0    4068    5739 
   Size:       4068    1671
 
NgoMIV G'CCGG_C

Cuts at:      0     557    1687    2365    5739 
   Size:        557    1130     678    3374

  Fragments arranged by size: 

                3374    1130     678     557
 
NheI G'CTAG_C

Cuts at:      0    3184    5739 
   Size:       3184    2555
 
NruI TCG'CGA

Cuts at:      0    5444    5739 
   Size:       5444     295
 
NspI r_CATG'y

Cuts at:      0     645    2365    4151    4698    4932    5739 
   Size:        645    1720    1786     547     234     807

  Fragments arranged by size: 

                1786    1720     807     645     547     234
 
PasI CC'CwG_GG

Cuts at:      0     920    2199    5739 
   Size:        920    1279    3540

  Fragments arranged by size: 

                3540    1279     920
 
PciI A'CATG_T

Cuts at:      0    4928    5739 
   Size:       4928     811
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     756    3389    4572    5739 
   Size:        756    2633    1183    1167

  Fragments arranged by size: 

                2633    1183    1167     756
 
PfoI T'CCnGG_A

Cuts at:      0    1312    1343    1709    2045    2057    3607    3833    4172 
   Size:       1312      31     366     336      12    1550     226     339

Cuts at:   4172    4610    5739 
   Size:        438    1129

  Fragments arranged by size: 

                1550    1312    1129     438     366     339     336     226
                  31      12
 
PmlI CAC'GTG

Cuts at:      0     695    5739 
   Size:        695    5044
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2311    2343    5739 
   Size:       2311      32    3396

  Fragments arranged by size: 

                3396    2311      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1086    1616    2487    2540    4176    4857    5739 
   Size:       1086     530     871      53    1636     681     882

  Fragments arranged by size: 

                1636    1086     882     871     681     530      53
 
PshAI GACnn'nnGTC

Cuts at:      0    1006    4817    5739 
   Size:       1006    3811     922

  Fragments arranged by size: 

                3811    1006     922
 
PspOMI G'GGCC_C

Cuts at:      0    3061    5739 
   Size:       3061    2678
 
PstI C_TGCA'G

Cuts at:      0     605     873    1043    1601    1783    1880    2789    5739 
   Size:        605     268     170     558     182      97     909    2950

  Fragments arranged by size: 

                2950     909     605     558     268     182     170      97
            
 
PvuII CAG'CTG

Cuts at:      0     736    1457    2480    2682    4003    5739 
   Size:        736     721    1023     202    1321    1736

  Fragments arranged by size: 

                1736    1321    1023     736     721     202
 
SacI G_AGCT'C

Cuts at:      0    1703    3625    4036    5687    5739 
   Size:       1703    1922     411    1651      52

  Fragments arranged by size: 

                1922    1703    1651     411      52
 
SanDI GG'GwC_CC

Cuts at:      0    1616    2540    5739 
   Size:       1616     924    3199

  Fragments arranged by size: 

                3199    1616     924
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4280    5739 
   Size:       4280    1459
 
SbfI CC_TGCA'GG

Cuts at:      0    1043    1880    5739 
   Size:       1043     837    3859

  Fragments arranged by size: 

                3859    1043     837
 
SfcI C'TryA_G

Cuts at:      0     601     869    1039    1491    1597    1779    1876    2431 
   Size:        601     268     170     452     106     182      97     555

Cuts at:   2431    2785    4095    5739 
   Size:        354    1310    1644

  Fragments arranged by size: 

                1644    1310     601     555     452     354     268     182
                 170     106      97
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2556    5739 
   Size:       2556    3183
 
SmlI C'TyrA_G

Cuts at:      0      63     583    2386    3199    3412    4756    5386    5739 
   Size:         63     520    1803     813     213    1344     630     353

  Fragments arranged by size: 

                1803    1344     813     630     520     353     213      63
            
 
SphI G_CATG'C

Cuts at:      0     645    2365    5739 
   Size:        645    1720    3374

  Fragments arranged by size: 

                3374    1720     645
 
StuI AGG'CCT

Cuts at:      0     724    1079    2089    2550    5739 
   Size:        724     355    1010     461    3189

  Fragments arranged by size: 

                3189    1010     724     461     355
 
StyI C'CwwG_G

Cuts at:      0     660     750     949    1211    1476    1731    1763    3014 
   Size:        660      90     199     262     265     255      32    1251

Cuts at:   3014    3146    3587    5006    5075    5149    5195    5739 
   Size:        132     441    1419      69      74      46     544

  Fragments arranged by size: 

                1419    1251     660     544     441     265     262     255
                 199     132      90      74      69      46      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1881    2131    2170    2944    5739 
   Size:       1881     250      39     774    2795

  Fragments arranged by size: 

                2795    1881     774     250      39
 
TatI w'GTAC_w

Cuts at:      0     689    2716    4086    4691    4721    5213    5633    5739 
   Size:        689    2027    1370     605      30     492     420     106

  Fragments arranged by size: 

                2027    1370     689     605     492     420     106      30
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1856    1888    3746    3778    4666    4698    5739 
   Size:       1856      32    1858      32     888      32    1041

  Fragments arranged by size: 

                1858    1856    1041     888      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2811    4047    5739 
   Size:       2811    1236    1692

  Fragments arranged by size: 

                2811    1692    1236
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     753    4602    5739 
   Size:        753    3849    1137

  Fragments arranged by size: 

                3849    1137     753
 
XmnI GAAnn'nnTTC

Cuts at:      0    4480    5739 
   Size:       4480    1259
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FseI    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SanDI     SapI     SbfI     SfcI
     SfiI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII     AgeI     AhdI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BfrBI    BsaHI    BsiEI    BspEI    BssSI
    BtgZI     ClaI     DraI     DrdI     EagI    EcoRI    EcoRV     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     NarI     NotI
     NsiI     PacI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
     ZraI
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