Restriction map for pF1KB1440
##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        AscI        
AseI        AsnI        Asp700I     AspEI       AvrII       
BcgI        BfrI        BglII       BlnI        BsaBI       
BsmBI       BssHII      Bst1107I    BstBI       BstZ17I     
DraI        Eam1105I    EcoRV       EcoT22I     HpaI        
MamI        MfeI        MluI        MunI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    SbfI        
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SspI        SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       3381  GGTACC               G^GTACC
AcsI         4077  GAATTC               [GA]^AATT[CT]
ApoI         4077  GAATTC               [GA]^AATT[CT]
Asp718I      3381  GGTACC               G^GTACC
AspI         2754  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        1336  GGATAC               GTATCC(6/5)
BsiWI         870  CGTACG               C^GTACG
BsmI         4224  GAATGC               GAATGC(1/-1)
BspHI        4313  TCATGA               T^CATGA
BspLU11I     5570  ACATGT               A^CATGT
CpoI         1865  CGGTCCG              CG^G[AT]CCG
EcoRI        4077  GAATTC               G^AATTC
FseI         2437  GGCCGGCC             GGCCGG^CC
HindIII      5191  AAGCTT               A^AGCTT
KpnI         3381  GGTACC               GGTAC^C
NaeI         2438  GCCGGC               GCC^GGC
NdeI         3527  CATATG               CA^TATG
NgoMIV       2438  GCCGGC               G^CCGGC
NheI         2298  GCTAGC               G^CTAGC
PflFI        2754  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI         3869  CGATCG               CGAT^CG
RcaI         4313  TCATGA               T^CATGA
RsrII        1865  CGGTCCG              CG^G[AT]CCG
SalI         5566  GTCGAC               G^TCGAC
SapI          949  GCTCTTC              GCTCTTC(1/4)
SpeI          352  ACTAGT               A^CTAGT
Tth111I      2754  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3459  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AatII        2290  GACGTC               GACGT^C
             3048  GACGTC              
AccIII       1356  TCCGGA               T^CCGGA
             3618  TCCGGA              
AocI          115  CCTGAGG              CC^T[ACGT]AGG
             1606  CCTCAGG             
AviII        2102  TGCGCA               TGC^GCA
             4442  TGCGCA              
BamHI         275  GGATCC               G^GATCC
             1580  GGATCC              
BbeI           76  GGCGCC               GGCGC^C
             1233  GGCGCC              
BbvCI         421  CCTCAGC              CCTCAGC(-5/-2)
             1134  CCTCAGC             
BclI         2453  TGATCA               T^GATCA
             4353  TGATCA              
BsaWI        1356  TCCGGA               [AT]^CCGG[AT]
             3618  TCCGGA              
BseAI        1356  TCCGGA               T^CCGGA
             3618  TCCGGA              
Bsp1407I      319  TGTACA               T^GTACA
             3113  TGTACA              
BspDI        4162  ATCGAT               AT^CGAT
             4204  ATCGAT              
BspEI        1356  TCCGGA               T^CCGGA
             3618  TCCGGA              
BsrBI        1612  GAGCGG               CCGCTC(-3/-3)
             4669  GAGCGG              
BsrGI         319  TGTACA               T^GTACA
             3113  TGTACA              
Bsu36I        115  CCTGAGG              CC^T[ACGT]AGG
             1606  CCTCAGG             
ClaI         4162  ATCGAT               AT^CGAT
             4204  ATCGAT              
DraIII       1669  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3238  CACCTGGTG           
DrdI          126  GACTGGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2926  GACATCAAGGTC        
EagI          543  CGGCCG               C^GGCCG
             3312  CGGCCG              
EarI          950  CTCTTC               CTCTTC(1/4)
              376  GAAGAG              
EclXI         543  CGGCCG               C^GGCCG
             3312  CGGCCG              
Eco52I        543  CGGCCG               C^GGCCG
             3312  CGGCCG              
Eco81I        115  CCTGAGG              CC^T[ACGT]AGG
             1606  CCTCAGG             
EcoNI        2616  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4392  CCTTATTCAGG         
FbaI         2453  TGATCA               T^GATCA
             4353  TGATCA              
FspI         2102  TGCGCA               TGC^GCA
             4442  TGCGCA              
HincII       5269  GTTGAC               GT[CT]^[GA]AC
             5566  GTCGAC              
HindII       5269  GTTGAC               GT[CT]^[GA]AC
             5566  GTCGAC              
KasI           76  GGCGCC               G^GCGCC
             1233  GGCGCC              
Ksp632I       950  CTCTTC               CTCTTC(1/4)
              376  GAAGAG              
MroI         1356  TCCGGA               T^CCGGA
             3618  TCCGGA              
MseI          107  TTAA                 T^TAA
             3104  TTAA                
NarI           76  GGCGCC               GG^CGCC
             1233  GGCGCC              
PaeR7I       4142  CTCGAG               C^TCGAG
             4894  CTCGAG              
SfoI           76  GGCGCC               GGC^GCC
             1233  GGCGCC              
SspBI         319  TGTACA               T^GTACA
             3113  TGTACA              
StuI         4574  AGGCCT               AGG^CCT
             5093  AGGCCT              
Tru9I         107  TTAA                 T^TAA
             3104  TTAA                
XhoI         4142  CTCGAG               C^TCGAG
             4894  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco47III    EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SacI        SacII       Sau3AI      
Sau96I      ScaI        ScrFI       SexAI       SfaNI       
SfcI        SmaI        SmlI        SphI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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