Restriction map for pF1KB1440
 (Linear) (Six-base) MAPSORT of: KIBB1440.seq  Check: 5694  from: 1  to: 5694



KIBB1440.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    5424    5694 
   Size:       5424     270
 
AatII G_ACGT'C

Cuts at:      0    2294    3052    5694 
   Size:       2294     758    2642

  Fragments arranged by size: 

                2642    2294     758
 
AccI GT'mk_AC

Cuts at:      0     524    1346    2180    2761    5567    5694 
   Size:        524     822     834     581    2806     127

  Fragments arranged by size: 

                2806     834     822     581     524     127
 
Acc65I G'GTAC_C

Cuts at:      0    3381    5694 
   Size:       3381    2313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1735    3541    3898    5694 
   Size:        448    1287    1806     357    1796

  Fragments arranged by size: 

                1806    1796    1287     448     357
 
AfeI AGC'GCT

Cuts at:      0     229    3679    5242    5694 
   Size:        229    3450    1563     452

  Fragments arranged by size: 

                3450    1563     452     229
 
AflIII A'CryG_T

Cuts at:      0     643    4763    4928    5570    5694 
   Size:        643    4120     165     642     124

  Fragments arranged by size: 

                4120     643     642     165     124
 
AleI CACnn'nnGTG

Cuts at:      0     892    1420    1477    2065    2170    2242    2830    4463 
   Size:        892     528      57     588     105      72     588    1633

Cuts at:   4463    4813    5489    5512    5694 
   Size:        350     676      23     182

  Fragments arranged by size: 

                1633     892     676     588     588     528     350     182
                 105      72      57      23
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1450    2110    3029    5507    5694 
   Size:       1450     660     919    2478     187

  Fragments arranged by size: 

                2478    1450     919     660     187
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4882    4914    5694 
   Size:       4882      32     780

  Fragments arranged by size: 

                4882     780      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     490     658    2862    3240    4851    5694 
   Size:        490     168    2204     378    1611     843

  Fragments arranged by size: 

                2204    1611     843     490     378     168
 
ApaI G_GGCC'C

Cuts at:      0    1084    2074    4249    4601    5694 
   Size:       1084     990    2175     352    1093

  Fragments arranged by size: 

                2175    1093    1084     990     352
 
ApaLI G'TGCA_C

Cuts at:      0     770    4609    5482    5694 
   Size:        770    3839     873     212

  Fragments arranged by size: 

                3839     873     770     212
 
ApoI r'AATT_y

Cuts at:      0    4077    5694 
   Size:       4077    1617
 
AvaI C'yCGr_G

Cuts at:      0    1309    2080    2704    3433    4142    4174    4255    4340 
   Size:       1309     771     624     729     709      32      81      85

Cuts at:   4340    4894    5333    5694 
   Size:        554     439     361

  Fragments arranged by size: 

                1309     771     729     709     624     554     439     361
                  85      81      32
 
BamHI G'GATC_C

Cuts at:      0     275    1580    5694 
   Size:        275    1305    4114

  Fragments arranged by size: 

                4114    1305     275
 
BanI G'GyrC_C

Cuts at:      0      33      76     568    1233    2389    2492    3381    4375 
   Size:         33      43     492     665    1156     103     889     994

Cuts at:   4375    4815    5694 
   Size:        440     879

  Fragments arranged by size: 

                1156     994     889     879     665     492     440     103
                  43      33
 
BanII G_rGCy'C

Cuts at:      0     815     860    1084    1319    1412    1858    2074    2267 
   Size:        815      45     224     235      93     446     216     193

Cuts at:   2267    4249    4601    4895    5047    5694 
   Size:       1982     352     294     152     647

  Fragments arranged by size: 

                1982     815     647     446     352     294     235     224
                 216     193     152      93      45
 
BbeI G_GCGC'C

Cuts at:      0      80    1237    5694 
   Size:         80    1157    4457

  Fragments arranged by size: 

                4457    1157      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      93    1664    1767    3052    4693    5593    5694 
   Size:         93    1571     103    1285    1641     900     101

  Fragments arranged by size: 

                1641    1571    1285     900     103     101      93
 
BbvCI CC'TCA_GC

Cuts at:      0     422    1135    5694 
   Size:        422     713    4559

  Fragments arranged by size: 

                4559     713     422
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1330    5694 
   Size:       1330    4364
 
BclI T'GATC_A

Cuts at:      0    2453    4353    5694 
   Size:       2453    1900    1341

  Fragments arranged by size: 

                2453    1900    1341
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1907    1939    2127    2557    4637    5694 
   Size:       1907      32     188     430    2080    1057

  Fragments arranged by size: 

                2080    1907    1057     430     188      32
 
BlpI GC'TnA_GC

Cuts at:      0     859    1273    3061    5694 
   Size:        859     414    1788    2633

  Fragments arranged by size: 

                2633    1788     859     414
 
Bme1580I G_kGCm'C

Cuts at:      0      38     774    1075    1084    1218    2058    2074    2123 
   Size:         38     736     301       9     134     840      16      49

Cuts at:   2123    2394    3101    3146    4249    4601    4613    5399    5486 
   Size:        271     707      45    1103     352      12     786      87

Cuts at:   5486    5694 
   Size:        208

  Fragments arranged by size: 

                1103     840     786     736     707     352     301     271
                 208     134      87      49      45      38      16      12
                   9
 
BmgBI CAC'GTC

Cuts at:      0    2067    2118    2759    4931    5694 
   Size:       2067      51     641    2172     763

  Fragments arranged by size: 

                2172    2067     763     641      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     137    1650    1800    1914    5292    5694 
   Size:        137    1513     150     114    3378     402

  Fragments arranged by size: 

                3378    1513     402     150     137     114
 
BmtI G_CTAG'C

Cuts at:      0    2302    5694 
   Size:       2302    3392
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1224    1983    2041    2272    2470    3463    3672 
   Size:        874     350     759      58     231     198     993     209

Cuts at:   3672    4108    4296    4348    4455    4807    4850    5560    5680 
   Size:        436     188      52     107     352      43     710     120

Cuts at:   5680    5694 
   Size:         14

  Fragments arranged by size: 

                 993     874     759     710     436     352     350     231
                 209     198     188     120     107      58      52      43
                  14
 
Bpu10I CC'TnA_GC

Cuts at:      0       8     422    1135    5694 
   Size:          8     414     713    4559

  Fragments arranged by size: 

                4559     713     414       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2890    3645    4006    5694 
   Size:       2890     755     361    1688

  Fragments arranged by size: 

                2890    1688     755     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2335    3947    4252    4375    5694 
   Size:       2335    1612     305     123    1319

  Fragments arranged by size: 

                2335    1612    1319     305     123
 
BsaAI yAC'GTr

Cuts at:      0     786    2244    4070    4710    4764    4799    5694 
   Size:        786    1458    1826     640      54      35     895

  Fragments arranged by size: 

                1826    1458     895     786     640      54      35
 
BsaHI Gr'CG_yC

Cuts at:      0      77    1234    1772    2291    3049    3415    4169    5694 
   Size:         77    1157     538     519     758     366     754    1525

  Fragments arranged by size: 

                1525    1157     758     754     538     519     366      77
            
 
BsaWI w'CCGG_w

Cuts at:      0    1356    3618    5694 
   Size:       1356    2262    2076

  Fragments arranged by size: 

                2262    2076    1356
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1675    1705    2995    3025    3742    3772    3828    3858 
   Size:       1675      30    1290      30     717      30      56      30

Cuts at:   3858    4027    4057    4321    4351    4744    4774    4882    4912 
   Size:        169      30     264      30     393      30     108      30

Cuts at:   4912    5694 
   Size:        782

  Fragments arranged by size: 

                1675    1290     782     717     393     264     169     108
                  56      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     683     823     894    1327    1402    1683    2692    3499 
   Size:        683     140      71     433      75     281    1009     807

Cuts at:   3499    3757    3891    4345    5694 
   Size:        258     134     454    1349

  Fragments arranged by size: 

                1349    1009     807     683     454     433     281     258
                 140     134      75      71
 
BseYI C'CCAG_C

Cuts at:      0     224     712     909    1198    1495    1538    2003    2122 
   Size:        224     488     197     289     297      43     465     119

Cuts at:   2122    2200    2523    2716    2974    3065    3731    4851    5694 
   Size:         78     323     193     258      91     666    1120     843

  Fragments arranged by size: 

                1120     843     666     488     465     323     297     289
                 258     224     197     193     119      91      78      43
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     313    1279    1315    1733    2447    2602    4765 
   Size:         32     281     966      36     418     714     155    2163

Cuts at:   4765    5638    5694 
   Size:        873      56

  Fragments arranged by size: 

                2163     966     873     714     418     281     155      56
                  36      32
 
BsiEI CG_ry'CG

Cuts at:      0     546    1421    3315    3872    5694 
   Size:        546     875    1894     557    1822

  Fragments arranged by size: 

                1894    1822     875     557     546
 
BsiHKAI G_wGCw'C

Cuts at:      0     374     774     815     860    1997    3430    3554    4613 
   Size:        374     400      41      45    1137    1433     124    1059

Cuts at:   4613    4895    5131    5162    5486    5694 
   Size:        282     236      31     324     208

  Fragments arranged by size: 

                1433    1137    1059     400     374     324     282     236
                 208     124      45      41      31
 
BsiWI C'GTAC_G

Cuts at:      0     870    5694 
   Size:        870    4824
 
BsmI GAATG_Cn'

Cuts at:      0    4230    5694 
   Size:       4230    1464
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     374     774     815     860    1075    1084    1218 
   Size:         38     336     400      41      45     215       9     134

Cuts at:   1218    1319    1412    1858    1997    2058    2074    2123    2267 
   Size:        101      93     446     139      61      16      49     144

Cuts at:   2267    2394    3101    3146    3430    3554    4249    4601    4613 
   Size:        127     707      45     284     124     695     352      12

Cuts at:   4613    4895    5047    5131    5162    5399    5486    5694 
   Size:        282     152      84      31     237      87     208

  Fragments arranged by size: 

                 707     695     446     400     352     336     284     282
                 237     215     208     152     144     139     134     127
                 124     101      93      87      84      61      49      45
                  45      41      38      31      16      12       9
 
BspEI T'CCGG_A

Cuts at:      0    1356    3618    5694 
   Size:       1356    2262    2076

  Fragments arranged by size: 

                2262    2076    1356
 
BspHI T'CATG_A

Cuts at:      0    4313    5694 
   Size:       4313    1381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2720    2744    3179    4723    4961    5424    5694 
   Size:       2720      24     435    1544     238     463     270

  Fragments arranged by size: 

                2720    1544     463     435     270     238      24
 
BsrBI CCG'CTC

Cuts at:      0    1614    4671    5694 
   Size:       1614    3057    1023

  Fragments arranged by size: 

                3057    1614    1023
 
BsrDI GCAATG_nn'

Cuts at:      0     283     870    3047    5694 
   Size:        283     587    2177    2647

  Fragments arranged by size: 

                2647    2177     587     283
 
BsrFI r'CCGG_y

Cuts at:      0    2438    2531    2611    2997    3605    4636    4976    5694 
   Size:       2438      93      80     386     608    1031     340     718

  Fragments arranged by size: 

                2438    1031     718     608     386     340      93      80
            
 
BsrGI T'GTAC_A

Cuts at:      0     319    3113    5694 
   Size:        319    2794    2581

  Fragments arranged by size: 

                2794    2581     319
 
BssSI C'ACGA_G

Cuts at:      0     534    1590    4681    5694 
   Size:        534    1056    3091    1013

  Fragments arranged by size: 

                3091    1056    1013     534
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     769    1530    3150    4640    5394    5694 
   Size:        769     761    1620    1490     754     300

  Fragments arranged by size: 

                1620    1490     769     761     754     300
 
BstEII G'GTnAC_C

Cuts at:      0    1674    2235    4401    5694 
   Size:       1674     561    2166    1293

  Fragments arranged by size: 

                2166    1674    1293     561
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     414    1326    2772    3000    4465    5070    5694 
   Size:        414     912    1446     228    1465     605     624

  Fragments arranged by size: 

                1465    1446     912     624     605     414     228
 
BstYI r'GATC_y

Cuts at:      0     275    1580    2219    5694 
   Size:        275    1305     639    3475

  Fragments arranged by size: 

                3475    1305     639     275
 
Bsu36I CC'TnA_GG

Cuts at:      0     116    1607    5694 
   Size:        116    1491    4087

  Fragments arranged by size: 

                4087    1491     116
 
BtgI C'CryG_G

Cuts at:      0      81    1421    1478    1544    1862    1928    1959    2134 
   Size:         81    1340      57      66     318      66      31     175

Cuts at:   2134    2223    2268    2462    2828    3210    3665    4262    4371 
   Size:         89      45     194     366     382     455     597     109

Cuts at:   4371    4521    4601    4886    5031    5612    5694 
   Size:        150      80     285     145     581      82

  Fragments arranged by size: 

                1340     597     581     455     382     366     318     285
                 194     175     150     145     109      89      82      81
                  80      66      66      57      45      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1499    3792    4110    4218    5694 
   Size:       1499    2293     318     108    1476

  Fragments arranged by size: 

                2293    1499    1476     318     108
 
BtsI GCAGTG_nn'

Cuts at:      0     594     770    1214    1303    2165    2474    4611    5484 
   Size:        594     176     444      89     862     309    2137     873

Cuts at:   5484    5496    5694 
   Size:         12     198

  Fragments arranged by size: 

                2137     873     862     594     444     309     198     176
                  89      12
 
ClaI AT'CG_AT

Cuts at:      0    4163    4205    5694 
   Size:       4163      42    1489

  Fragments arranged by size: 

                4163    1489      42
 
DraIII CAC_nnn'GTG

Cuts at:      0    1674    3243    5694 
   Size:       1674    1569    2451

  Fragments arranged by size: 

                2451    1674    1569
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     132    2932    5694 
   Size:        132    2800    2762

  Fragments arranged by size: 

                2800    2762     132
 
EaeI y'GGCC_r

Cuts at:      0     543     840     896    1106    2214    2465    3312    4301 
   Size:        543     297      56     210    1108     251     847     989

Cuts at:   4301    4363    4427    4513    4704    4803    4910    5230    5380 
   Size:         62      64      86     191      99     107     320     150

Cuts at:   5380    5694 
   Size:        314

  Fragments arranged by size: 

                1108     989     847     543     320     314     297     251
                 210     191     150     107      99      86      64      62
                  56
 
EagI C'GGCC_G

Cuts at:      0     543    3312    5694 
   Size:        543    2769    2382

  Fragments arranged by size: 

                2769    2382     543
 
EarI CTCTTCn'nnn_

Cuts at:      0     371     956    5694 
   Size:        371     585    4738

  Fragments arranged by size: 

                4738     585     371
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     491    1809    2812    3494    4000    5694 
   Size:        491    1318    1003     682     506    1694

  Fragments arranged by size: 

                1694    1318    1003     682     506     491
 
EcoICRI GAG'CTC

Cuts at:      0     813     858    4893    5694 
   Size:        813      45    4035     801

  Fragments arranged by size: 

                4035     813     801      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     874    1224    1735    1983    2041    2272    2470 
   Size:        448     426     350     511     248      58     231     198

Cuts at:   2470    3463    3541    3672    3898    4108    4296    4348    4455 
   Size:        993      78     131     226     210     188      52     107

Cuts at:   4455    4807    4850    5560    5680    5694 
   Size:        352      43     710     120      14

  Fragments arranged by size: 

                 993     710     511     448     426     352     350     248
                 231     226     210     198     188     131     120     107
                  78      58      52      43      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2620    4396    5694 
   Size:       2620    1776    1298

  Fragments arranged by size: 

                2620    1776    1298
 
EcoO109I rG'GnC_Cy

Cuts at:      0     418    1080    1081    1710    1881    2007    2071    2699 
   Size:        418     662       1     629     171     126      64     628

Cuts at:   2699    2709    2781    3228    3769    3836    4245    4246    4597 
   Size:         10      72     447     541      67     409       1     351

Cuts at:   4597    5113    5694 
   Size:        516     581

  Fragments arranged by size: 

                 662     629     628     581     541     516     447     418
                 409     351     171     126      72      67      64      10
                   1       1
 
EcoRI G'AATT_C

Cuts at:      0    4077    5694 
   Size:       4077    1617
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1695    3903    5694 
   Size:       1695    2208    1791

  Fragments arranged by size: 

                2208    1791    1695
 
FseI GG_CCGG'CC

Cuts at:      0    2442    5694 
   Size:       2442    3252
 
FspI TGC'GCA

Cuts at:      0    2104    4444    5694 
   Size:       2104    2340    1250

  Fragments arranged by size: 

                2340    2104    1250
 
FspAI rTGC'GCAy

Cuts at:      0    2104    4444    5694 
   Size:       2104    2340    1250

  Fragments arranged by size: 

                2340    2104    1250
 
HaeII r_GCGC'y

Cuts at:      0      80     231    1237    1280    3681    4002    4783    4949 
   Size:         80     151    1006      43    2401     321     781     166

Cuts at:   4949    5244    5694 
   Size:        295     450

  Fragments arranged by size: 

                2401    1006     781     450     321     295     166     151
                  80      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1328    1360    1552    1584    1675    1707    2264    2296 
   Size:       1328      32     192      32      91      32     557      32

Cuts at:   2296    3130    3162    3828    3850    3860    3882    4027    4059 
   Size:        834      32     666      22      10      22     145      32

Cuts at:   4059    4321    4353    5329    5361    5694 
   Size:        262      32     976      32     333

  Fragments arranged by size: 

                1328     976     834     666     557     333     262     192
                 145      91      32      32      32      32      32      32
                  32      32      22      22      10
 
HincII GTy'rAC

Cuts at:      0    5271    5568    5694 
   Size:       5271     297     126

  Fragments arranged by size: 

                5271     297     126
 
HindIII A'AGCT_T

Cuts at:      0    5191    5694 
   Size:       5191     503
 
KasI G'GCGC_C

Cuts at:      0      76    1233    5694 
   Size:         76    1157    4461

  Fragments arranged by size: 

                4461    1157      76
 
KpnI G_GTAC'C

Cuts at:      0    3385    5694 
   Size:       3385    2309
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    1550    4391    4646    5694 
   Size:        623     927    2841     255    1048

  Fragments arranged by size: 

                2841    1048     927     623     255
 
MscI TGG'CCA

Cuts at:      0     898    1108    2216    4365    4429    4515    4706    4912 
   Size:        898     210    1108    2149      64      86     191     206

Cuts at:   4912    5232    5382    5694 
   Size:        320     150     312

  Fragments arranged by size: 

                2149    1108     898     320     312     210     206     191
                 150      86      64
 
MslI CAynn'nnrTG

Cuts at:      0     196     412     892    1291    1420    1477    2065    2170 
   Size:        196     216     480     399     129      57     588     105

Cuts at:   2170    2242    2449    2467    2830    2998    3376    4463    4606 
   Size:         72     207      18     363     168     378    1087     143

Cuts at:   4606    4813    5179    5489    5512    5694 
   Size:        207     366     310      23     182

  Fragments arranged by size: 

                1087     588     480     399     378     366     363     310
                 216     207     207     196     182     168     143     129
                 105      72      57      23      18
 
MspA1I CmG'CkG

Cuts at:      0     387     426     558    1069    1546    1743    1961    2003 
   Size:        387      39     132     511     477     197     218      42

Cuts at:   2003    2126    2136    2225    2327    2720    3065    3094    3285 
   Size:        123      10      89     102     393     345      29     191

Cuts at:   3285    3492    5640    5694 
   Size:        207    2148      54

  Fragments arranged by size: 

                2148     511     477     393     387     345     218     207
                 197     191     132     123     102      89      54      42
                  39      29      10
 
NaeI GCC'GGC

Cuts at:      0    2440    5694 
   Size:       2440    3254
 
NarI GG'CG_CC

Cuts at:      0      77    1234    5694 
   Size:         77    1157    4460

  Fragments arranged by size: 

                4460    1157      77
 
NcoI C'CATG_G

Cuts at:      0      81    3665    4262    4521    4601    4886    5612    5694 
   Size:         81    3584     597     259      80     285     726      82

  Fragments arranged by size: 

                3584     726     597     285     259      82      81      80
            
 
NdeI CA'TA_TG

Cuts at:      0    3528    5694 
   Size:       3528    2166
 
NgoMIV G'CCGG_C

Cuts at:      0    2438    5694 
   Size:       2438    3256
 
NheI G'CTAG_C

Cuts at:      0    2298    5694 
   Size:       2298    3396
 
NspI r_CATG'y

Cuts at:      0     758    1272    1972    2179    2743    3411    4722    5151 
   Size:        758     514     700     207     564     668    1311     429

Cuts at:   5151    5295    5550    5574    5694 
   Size:        144     255      24     120

  Fragments arranged by size: 

                1311     758     700     668     564     514     429     255
                 207     144     120      24
 
PasI CC'CwG_GG

Cuts at:      0      19     394    5694 
   Size:         19     375    5300

  Fragments arranged by size: 

                5300     375      19
 
PciI A'CATG_T

Cuts at:      0    5570    5694 
   Size:       5570     124
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4372    4522    4851    5694 
   Size:       4372     150     329     843

  Fragments arranged by size: 

                4372     843     329     150
 
PfoI T'CCnGG_A

Cuts at:      0     851    2986    3554    5069    5537    5694 
   Size:        851    2135     568    1515     468     157

  Fragments arranged by size: 

                2135    1515     851     568     468     157
 
PmlI CAC'GTG

Cuts at:      0    4070    4710    4799    5694 
   Size:       4070     640      89     895

  Fragments arranged by size: 

                4070     895     640      89
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4882    4914    5694 
   Size:       4882      32     780

  Fragments arranged by size: 

                4882     780      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1710    1881    2007    2699    2709    2781    3769    3836 
   Size:       1710     171     126     692      10      72     988      67

Cuts at:   3836    5694 
   Size:       1858

  Fragments arranged by size: 

                1858    1710     988     692     171     126      72      67
                  10
 
PspOMI G'GGCC_C

Cuts at:      0    1080    2070    4245    4597    5694 
   Size:       1080     990    2175     352    1097

  Fragments arranged by size: 

                2175    1097    1080     990     352
 
PspXI vC'TCGA_Gb

Cuts at:      0    4894    5694 
   Size:       4894     800
 
PstI C_TGCA'G

Cuts at:      0    2540    2867    3176    3236    4618    5024    5491    5694 
   Size:       2540     327     309      60    1382     406     467     203

  Fragments arranged by size: 

                2540    1382     467     406     327     309     203      60
            
 
PvuI CG_AT'CG

Cuts at:      0    3872    5694 
   Size:       3872    1822
 
PvuII CAG'CTG

Cuts at:      0     387     426     558    1743    2003    3065    3094    5640 
   Size:        387      39     132    1185     260    1062      29    2546

Cuts at:   5640    5694 
   Size:         54

  Fragments arranged by size: 

                2546    1185    1062     387     260     132      54      39
                  29
 
RsrII CG'GwC_CG

Cuts at:      0    1866    5694 
   Size:       1866    3828
 
SacI G_AGCT'C

Cuts at:      0     815     860    4895    5694 
   Size:        815      45    4035     799

  Fragments arranged by size: 

                4035     815     799      45
 
SacII CC_GC'GG

Cuts at:      0    1547    1962    2137    2226    5694 
   Size:       1547     415     175      89    3468

  Fragments arranged by size: 

                3468    1547     415     175      89
 
SalI G'TCGA_C

Cuts at:      0    5566    5694 
   Size:       5566     128
 
SanDI GG'GwC_CC

Cuts at:      0    1881    2781    3769    5694 
   Size:       1881     900     988    1925

  Fragments arranged by size: 

                1925    1881     988     900
 
SapI GCTCTTCn'nnn_

Cuts at:      0     956    5694 
   Size:        956    4738
 
ScaI AGT'ACT

Cuts at:      0    1924    2542    3190    4360    5227    5260    5694 
   Size:       1924     618     648    1170     867      33     434

  Fragments arranged by size: 

                1924    1170     867     648     618     434      33
 
SexAI A'CCwGG_T

Cuts at:      0    2009    2231    3239    5694 
   Size:       2009     222    1008    2455

  Fragments arranged by size: 

                2455    2009    1008     222
 
SfcI C'TryA_G

Cuts at:      0     167     521    1119    1449    2536    2863    3172    3232 
   Size:        167     354     598     330    1087     327     309      60

Cuts at:   3232    4614    5020    5487    5694 
   Size:       1382     406     467     207

  Fragments arranged by size: 

                1382    1087     598     467     406     354     330     327
                 309     207     167      60
 
SfoI GGC'GCC

Cuts at:      0      78    1235    5694 
   Size:         78    1157    4459

  Fragments arranged by size: 

                4459    1157      78
 
SmaI CCC'GGG

Cuts at:      0    4176    4342    5335    5694 
   Size:       4176     166     993     359

  Fragments arranged by size: 

                4176     993     359     166
 
SmlI C'TyrA_G

Cuts at:      0    2905    3624    4021    4142    4894    5694 
   Size:       2905     719     397     121     752     800

  Fragments arranged by size: 

                2905     800     752     719     397     121
 
SpeI A'CTAG_T

Cuts at:      0     352    5694 
   Size:        352    5342
 
SphI G_CATG'C

Cuts at:      0    1272    3411    4722    5151    5550    5694 
   Size:       1272    2139    1311     429     399     144

  Fragments arranged by size: 

                2139    1311    1272     429     399     144
 
StuI AGG'CCT

Cuts at:      0    4576    5095    5694 
   Size:       4576     519     599

  Fragments arranged by size: 

                4576     599     519
 
StyI C'CwwG_G

Cuts at:      0      81     603     818    2378    2501    3418    3569    3665 
   Size:         81     522     215    1560     123     917     151      96

Cuts at:   3665    4262    4521    4601    4886    4919    5447    5612    5694 
   Size:        597     259      80     285      33     528     165      82

  Fragments arranged by size: 

                1560     917     597     528     522     285     259     215
                 165     151     123      96      82      81      80      33
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328     757     789    1933    2037    2510    2514    2662 
   Size:        328     429      32    1144     104     473       4     148

Cuts at:   2662    3750    3974    3978    5694 
   Size:       1088     224       4    1716

  Fragments arranged by size: 

                1716    1144    1088     473     429     328     224     148
                 104      32       4       4
 
TatI w'GTAC_w

Cuts at:      0     319    1637    1922    2540    3113    3188    4358    5225 
   Size:        319    1318     285     618     573      75    1170     867

Cuts at:   5225    5258    5285    5694 
   Size:         33      27     409

  Fragments arranged by size: 

                1318    1170     867     618     573     409     319     285
                  75      33      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3229    3261    3742    3774    3832    3864    5694 
   Size:       3229      32     481      32      58      32    1830

  Fragments arranged by size: 

                3229    1830     481      58      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2757    5694 
   Size:       2757    2937
 
XbaI T'CTAG_A

Cuts at:      0    3459    5694 
   Size:       3459    2235
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     906    1287    2007    3393    3978    5034    5694 
   Size:        906     381     720    1386     585    1056     660

  Fragments arranged by size: 

                1386    1056     906     720     660     585     381
 
XhoI C'TCGA_G

Cuts at:      0    4142    4894    5694 
   Size:       4142     752     800

  Fragments arranged by size: 

                4142     800     752
 
XmaI C'CCGG_G

Cuts at:      0    4174    4340    5333    5694 
   Size:       4174     166     993     361

  Fragments arranged by size: 

                4174     993     361     166
 
ZraI GAC'GTC

Cuts at:      0    2292    3050    5694 
   Size:       2292     758    2644

  Fragments arranged by size: 

                2644    2292     758
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI    BfrBI    BglII     BplI    BsaBI    BsmBI   BssHII
    BstBI  BstZ17I    CspCI     DraI    EcoRV     HpaI     MfeI     MluI
     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI     PsrI
     SbfI     SfiI     SgfI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XmnI
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