Restriction map for pF1KB1380
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       FbaI        FseI        
FspI        HapII       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MluI        MluNI       MroI        MscI        
MspI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII        1342  CGCG                 CG^CG
AciI          794  GCGG                 CCGC(-3/-1)
AcyI         1847  GACGCC               G[GA]^CG[CT]C
AfaI         1900  GTAC                 GT^AC
Alw44I       1765  GTGCAC               G^TGCAC
AlwNI        1675  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          177  CCTAAGG              CC^T[ACGT]AGG
ApaI         1400  GGGCCC               GGGCC^C
ApaLI        1765  GTGCAC               G^TGCAC
AspHI        1765  GTGCAC               G[AT]GC[AT]^C
BanII        1400  GGGCCC               G[GA]GC[CT]^C
BciVI         162  GGATAC               GTATCC(6/5)
BglII        1071  AGATCT               A^GATCT
BsaHI        1847  GACGCC               G[GA]^CG[CT]C
BseRI        1039  GAGGAG               GAGGAG(10/8)
BsgI         1681  CTGCAC               GTGCAG(16/14)
BsiHKAI      1765  GTGCAC               G[AT]GC[AT]^C
BsmBI        1845  GAGACG               CGTCTC(1/5)
BsmFI         141  GGGAC                GGGAC(10/14)
Bsp1407I     1899  TGTACA               T^GTACA
BspMI         128  ACCTGC               ACCTGC(4/8)
BsrGI        1899  TGTACA               T^GTACA
BstUI        1342  CGCG                 CG^CG
BstXI         444  CCAGGGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        177  CCTAAGG              CC^T[ACGT]AGG
DraII        1400  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          920  CCATGG               C^C[GA][CT]GG
Eco81I        177  CCTAAGG              CC^T[ACGT]AGG
EcoO109I     1400  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       920  CCATGG               C^C[AT][AT]GG
EcoT22I      2005  ATGCAT               ATGCA^T
FauI          794  GCGGG                CCCGC(4/6)
HaeII        1988  GGCGCT               [GA]GCGC^[CT]
Hin1I        1847  GACGCC               G[GA]^CG[CT]C
MfeI         1620  CAATTG               C^AATTG
MspA1I       1815  CAGCTG               C[AC]G^C[GT]G
MunI         1620  CAATTG               C^AATTG
MvnI         1342  CGCG                 CG^CG
MwoI           63  GCTGGGGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          920  CCATGG               C^CATGG
NdeI         1087  CATATG               CA^TATG
NsiI         2005  ATGCAT               ATGCA^T
PvuII        1815  CAGCTG               CAG^CTG
RsaI         1900  GTAC                 GT^AC
SexAI         334  ACCAGGT              A^CC[AT]GGT
SspBI        1899  TGTACA               T^GTACA
StyI          920  CCATGG               C^C[AT][AT]GG
Tsp45I        617  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

BbsI          204  GAAGAC               GAAGAC(2/6)
               46  GTCTTC              
BbvI         1881  GCAGC                GCAGC(8/12)
               69  GCTGC               
BmyI         1400  GGGCCC               G[AGT]GC[ACT]^C
             1765  GTGCAC              
BpuAI         204  GAAGAC               GAAGAC(2/6)
               46  GTCTTC              
BsaAI         528  TACGTG               [CT]AC^GT[GA]
              957  TACGTG              
BsaBI         490  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2002  GATATGCATC          
BsaI         1611  GAGACC               GGTCTC(1/5)
              338  GGTCTC              
BsiYI         396  CCAGACTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              843  CCATACGGGGG         
BslI          396  CCAGACTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              843  CCATACGGGGG         
Bsp1286I     1400  GGGCCC               G[AGT]GC[ACT]^C
             1765  GTGCAC              
BsrDI          19  GCAATG               GCAATG(2/0)
              449  GCAATG              
BssKI         335  CCAGG                ^CC[ACGT]GG
              444  CCAGG               
BssSI        1152  CTCGTG               CACGAG(-5/-1)
             1656  CTCGTG              
BstF5I        464  CATCC                GGATG(2/0)
              197  GGATG               
BstNI         335  CCAGG                CC^[AT]GG
              444  CCAGG               
CfoI         1341  GCGC                 GCG^C
             1989  GCGC                
EcoRII        335  CCAGG                ^CC[AT]GG
              444  CCAGG               
Fnu4HI         69  GCTGC                GC^[ACGT]GC
             1881  GCAGC               
FokI          464  CATCC                GGATG(9/13)
              197  GGATG               
HgaI         1847  GACGC                GACGC(5/10)
             1343  GCGTC               
HhaI         1341  GCGC                 GCG^C
             1989  GCGC                
HinP1I       1341  GCGC                 G^CGC
             1989  GCGC                
HphI          382  GGTGA                GGTGA(8/7)
             1335  TCACC               
ItaI           69  GCTGC                GC^[ACGT]GC
             1881  GCAGC               
MaeIII        557  GTTAC                ^GT[ACGT]AC
              617  GTGAC               
MamI          490  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2002  GATATGCATC          
MvaI          335  CCAGG                CC^[AT]GG
              444  CCAGG               
ScrFI         335  CCAGG                CC^[ACGT]GG
              444  CCAGG               
SmlI          341  CTCAAG               C^T[CT][GA]AG
             2076  CTCAAG              
StuI          800  AGGCCT               AGG^CCT
             2072  AGGCCT              
TaqI           99  TCGA                 T^CGA
             2085  TCGA                
TseI           69  GCTGC                G^C[AT]GC
             1881  GCAGC               
TthHB8I        99  TCGA                 T^CGA
             2085  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AflIII      AluI        AlwI        ApoI        
Asp700I     AvaII       BpmI        BsaJI       BsmAI       
BsmI        BsrI        BstYI       Cfr13I      DdeI        
DpnII       EarI        EcoRI       HaeIII      HinfI       
Ksp632I     MaeII       MboI        MboII       MflI        
MnlI        MseI        MslI        NdeII       NlaIII      
NlaIV       PleI        SapI        Sau3AI      Sau96I      
SfaNI       TfiI        Tru9I       Tsp509I     TspRI       
XhoII       XmnI        
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