Restriction map for
pF1KB0869
(Linear) (Six-base) MAPSORT of: KIBB0869.seq Check: 372 from: 1 to: 3222
KIBB0869.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1492 3039 3222
Size: 1492 1547 183
Fragments arranged by size:
1547 1492 183
AatII G_ACGT'C
Cuts at: 0 2374 3222
Size: 2374 848
AccI GT'mk_AC
Cuts at: 0 1220 3222
Size: 1220 2002
Acc65I G'GTAC_C
Cuts at: 0 1299 2984 3222
Size: 1299 1685 238
Fragments arranged by size:
1685 1299 238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1471 2131 3222
Size: 219 1252 660 1091
Fragments arranged by size:
1252 1091 660 219
AfeI AGC'GCT
Cuts at: 0 512 1244 1858 3222
Size: 512 732 614 1364
Fragments arranged by size:
1364 732 614 512
AflIII A'CryG_T
Cuts at: 0 1958 2900 3222
Size: 1958 942 322
Fragments arranged by size:
1958 942 322
AgeI A'CCGG_T
Cuts at: 0 377 3222
Size: 377 2845
AleI CACnn'nnGTG
Cuts at: 0 928 1628 3222
Size: 928 700 1594
Fragments arranged by size:
1594 928 700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1345 2521 3222
Size: 1345 1176 701
Fragments arranged by size:
1345 1176 701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 87 119 2541 2573 3222
Size: 87 32 2422 32 649
Fragments arranged by size:
2422 649 87 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1392 1484 2036 2285 3222
Size: 1089 303 92 552 249 937
Fragments arranged by size:
1089 937 552 303 249 92
ApaI G_GGCC'C
Cuts at: 0 1001 1497 1753 3222
Size: 1001 496 256 1469
Fragments arranged by size:
1469 1001 496 256
ApoI r'AATT_y
Cuts at: 0 678 2220 3222
Size: 678 1542 1002
Fragments arranged by size:
1542 1002 678
AvaI C'yCGr_G
Cuts at: 0 752 3222
Size: 752 2470
AvrII C'CTAG_G
Cuts at: 0 591 3222
Size: 591 2631
BamHI G'GATC_C
Cuts at: 0 1681 3222
Size: 1681 1541
BanI G'GyrC_C
Cuts at: 0 1072 1299 1457 2275 2984 3222
Size: 1072 227 158 818 709 238
Fragments arranged by size:
1072 818 709 238 227 158
BanII G_rGCy'C
Cuts at: 0 536 1001 1349 1373 1497 1753 3222
Size: 536 465 348 24 124 256 1469
Fragments arranged by size:
1469 536 465 348 256 124 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 800 954 2122 2244 3178 3222
Size: 800 154 1168 122 934 44
Fragments arranged by size:
1168 934 800 154 122 44
BbvCI CC'TCA_GC
Cuts at: 0 948 3222
Size: 948 2274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1746 1780 2177 2211 3222
Size: 1746 34 397 34 1011
Fragments arranged by size:
1746 1011 397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1052 3222
Size: 1052 2170
BclI T'GATC_A
Cuts at: 0 456 3222
Size: 456 2766
BfrBI ATG'CAT
Cuts at: 0 403 1146 3222
Size: 403 743 2076
Fragments arranged by size:
2076 743 403
BglI GCCn_nnn'nGGC
Cuts at: 0 2209 3103 3222
Size: 2209 894 119
Fragments arranged by size:
2209 894 119
BglII A'GATC_T
Cuts at: 0 3198 3222
Size: 3198 24
BlpI GC'TnA_GC
Cuts at: 0 2926 3222
Size: 2926 296
Bme1580I G_kGCm'C
Cuts at: 0 1001 1178 1497 1753 3222
Size: 1001 177 319 256 1469
Fragments arranged by size:
1469 1001 319 256 177
BmgBI CAC'GTC
Cuts at: 0 568 1224 3222
Size: 568 656 1998
Fragments arranged by size:
1998 656 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 2509 3176 3222
Size: 2509 667 46
Fragments arranged by size:
2509 667 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1567 3222
Size: 726 841 1655
Fragments arranged by size:
1655 841 726
Bpu10I CC'TnA_GC
Cuts at: 0 893 948 970 1558 2105 3222
Size: 893 55 22 588 547 1117
Fragments arranged by size:
1117 893 588 547 55 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 268 1302 3222
Size: 18 250 1034 1920
Fragments arranged by size:
1920 1034 250 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 1643 1817 3222
Size: 1643 174 1405
Fragments arranged by size:
1643 1405 174
BsaAI yAC'GTr
Cuts at: 0 1095 1212 1434 3222
Size: 1095 117 222 1788
Fragments arranged by size:
1788 1095 222 117
BsaHI Gr'CG_yC
Cuts at: 0 1648 2371 2735 3222
Size: 1648 723 364 487
Fragments arranged by size:
1648 723 487 364
BsaWI w'CCGG_w
Cuts at: 0 377 2417 3222
Size: 377 2040 805
Fragments arranged by size:
2040 805 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 593 623 2065 2095 3222
Size: 593 30 1442 30 1127
Fragments arranged by size:
1442 1127 593 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 596 1564 2941 3222
Size: 505 91 968 1377 281
Fragments arranged by size:
1377 968 505 281 91
BseYI C'CCAG_C
Cuts at: 0 494 1514 1973 2323 3222
Size: 494 1020 459 350 899
Fragments arranged by size:
1020 899 494 459 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1249 1378 2078 2406 2632 3222
Size: 402 847 129 700 328 226 590
Fragments arranged by size:
847 700 590 402 328 226 129
BsiEI CG_ry'CG
Cuts at: 0 378 2815 3222
Size: 378 2437 407
Fragments arranged by size:
2437 407 378
BsiHKAI G_wGCw'C
Cuts at: 0 536 1349 1433 2791 3222
Size: 536 813 84 1358 431
Fragments arranged by size:
1358 813 536 431 84
BsmI GAATG_Cn'
Cuts at: 0 165 403 2015 2408 2465 2500 3222
Size: 165 238 1612 393 57 35 722
Fragments arranged by size:
1612 722 393 238 165 57 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3030 3222
Size: 3030 192
Bsp1286I G_dGCh'C
Cuts at: 0 536 1001 1178 1349 1373 1433 1497 1753
Size: 536 465 177 171 24 60 64 256
Cuts at: 1753 2791 3222
Size: 1038 431
Fragments arranged by size:
1038 536 465 431 256 177 171 64
60 24
BspHI T'CATG_A
Cuts at: 0 812 3222
Size: 812 2410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1492 3039 3222
Size: 1492 1547 183
Fragments arranged by size:
1547 1492 183
BsrBI CCG'CTC
Cuts at: 0 428 1703 2597 3222
Size: 428 1275 894 625
Fragments arranged by size:
1275 894 625 428
BsrDI GCAATG_nn'
Cuts at: 0 938 1938 2073 3222
Size: 938 1000 135 1149
Fragments arranged by size:
1149 1000 938 135
BsrFI r'CCGG_y
Cuts at: 0 377 2810 3222
Size: 377 2433 412
Fragments arranged by size:
2433 412 377
BssSI C'ACGA_G
Cuts at: 0 1806 3222
Size: 1806 1416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1392 1484 2786 3222
Size: 1392 92 1302 436
Fragments arranged by size:
1392 1302 436 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2693 3222
Size: 2693 529
BstYI r'GATC_y
Cuts at: 0 252 449 1388 1442 1681 2033 2860 3198
Size: 252 197 939 54 239 352 827 338
Cuts at: 3198 3222
Size: 24
Fragments arranged by size:
939 827 352 338 252 239 197 54
24
BtgI C'CryG_G
Cuts at: 0 551 1076 1613 1642 1676 2774 2801 2918
Size: 551 525 537 29 34 1098 27 117
Cuts at: 2918 3222
Size: 304
Fragments arranged by size:
1098 551 537 525 304 117 34 29
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 963 3222
Size: 963 2259
BtsI GCAGTG_nn'
Cuts at: 0 1576 1732 1747 3222
Size: 1576 156 15 1475
Fragments arranged by size:
1576 1475 156 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 1809 2376 3222
Size: 1809 567 846
Fragments arranged by size:
1809 846 567
EaeI y'GGCC_r
Cuts at: 0 1265 2166 2812 3222
Size: 1265 901 646 410
Fragments arranged by size:
1265 901 646 410
EagI C'GGCC_G
Cuts at: 0 2812 3222
Size: 2812 410
EarI CTCTTCn'nnn_
Cuts at: 0 238 1331 1354 1447 2651 2870 3222
Size: 238 1093 23 93 1204 219 352
Fragments arranged by size:
1204 1093 352 238 219 93 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1377 3222
Size: 1377 1845
EcoICRI GAG'CTC
Cuts at: 0 534 1347 3222
Size: 534 813 1875
Fragments arranged by size:
1875 813 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 726 1471 1567 2131 3222
Size: 219 507 745 96 564 1091
Fragments arranged by size:
1091 745 564 507 219 96
EcoO109I rG'GnC_Cy
Cuts at: 0 998 1086 1493 1500 1749 1750 1978 2591
Size: 998 88 407 7 249 1 228 613
Cuts at: 2591 3222
Size: 631
Fragments arranged by size:
998 631 613 407 249 228 88 7
1
HaeII r_GCGC'y
Cuts at: 0 514 783 977 1246 1676 1860 3222
Size: 514 269 194 269 430 184 1362
Fragments arranged by size:
1362 514 430 269 269 194 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 441 473 1590 1622 1673 1705
Size: 241 32 168 32 1117 32 51 32
Cuts at: 1705 2065 2097 2231 2263 2618 2650 2680 2712
Size: 360 32 134 32 355 32 30 32
Cuts at: 2712 3222
Size: 510
Fragments arranged by size:
1117 510 360 355 241 168 134 51
32 32 32 32 32 32 32 32
30
HincII GTy'rAC
Cuts at: 0 182 1221 2148 2349 3222
Size: 182 1039 927 201 873
Fragments arranged by size:
1039 927 873 201 182
HpaI GTT'AAC
Cuts at: 0 182 3222
Size: 182 3040
KpnI G_GTAC'C
Cuts at: 0 1303 2988 3222
Size: 1303 1685 234
Fragments arranged by size:
1685 1303 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 2678 2687 3222
Size: 1621 1057 9 535
Fragments arranged by size:
1621 1057 535 9
MscI TGG'CCA
Cuts at: 0 1267 3222
Size: 1267 1955
MslI CAynn'nnrTG
Cuts at: 0 355 722 811 928 1141 1628 1664 3222
Size: 355 367 89 117 213 487 36 1558
Fragments arranged by size:
1558 487 367 355 213 117 89 36
MspA1I CmG'CkG
Cuts at: 0 479 523 952 1325 2982 3222
Size: 479 44 429 373 1657 240
Fragments arranged by size:
1657 479 429 373 240 44
NaeI GCC'GGC
Cuts at: 0 2812 3222
Size: 2812 410
NcoI C'CATG_G
Cuts at: 0 551 2774 3222
Size: 551 2223 448
Fragments arranged by size:
2223 551 448
NgoMIV G'CCGG_C
Cuts at: 0 2810 3222
Size: 2810 412
NsiI A_TGCA'T
Cuts at: 0 405 1148 3222
Size: 405 743 2074
Fragments arranged by size:
2074 743 405
NspI r_CATG'y
Cuts at: 0 1962 2198 2570 2904 3222
Size: 1962 236 372 334 318
Fragments arranged by size:
1962 372 334 318 236
PasI CC'CwG_GG
Cuts at: 0 72 3222
Size: 72 3150
PciI A'CATG_T
Cuts at: 0 1958 2900 3222
Size: 1958 942 322
Fragments arranged by size:
1958 942 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 2126 2521 3222
Size: 273 1853 395 701
Fragments arranged by size:
1853 701 395 273
PfoI T'CCnGG_A
Cuts at: 0 561 1777 2791 3222
Size: 561 1216 1014 431
Fragments arranged by size:
1216 1014 561 431
PmlI CAC'GTG
Cuts at: 0 1212 1434 3222
Size: 1212 222 1788
Fragments arranged by size:
1788 1212 222
PpuMI rG'GwC_Cy
Cuts at: 0 1500 1978 2591 3222
Size: 1500 478 613 631
Fragments arranged by size:
1500 631 613 478
PshAI GACnn'nnGTC
Cuts at: 0 2143 3222
Size: 2143 1079
PspOMI G'GGCC_C
Cuts at: 0 997 1493 1749 3222
Size: 997 496 256 1473
Fragments arranged by size:
1473 997 496 256
PstI C_TGCA'G
Cuts at: 0 1121 1388 1822 1867 2401 3222
Size: 1121 267 434 45 534 821
Fragments arranged by size:
1121 821 534 434 267 45
PvuII CAG'CTG
Cuts at: 0 523 952 1325 3222
Size: 523 429 373 1897
Fragments arranged by size:
1897 523 429 373
SacI G_AGCT'C
Cuts at: 0 536 1349 3222
Size: 536 813 1873
Fragments arranged by size:
1873 813 536
SalI G'TCGA_C
Cuts at: 0 1219 3222
Size: 1219 2003
SapI GCTCTTCn'nnn_
Cuts at: 0 1354 3222
Size: 1354 1868
ScaI AGT'ACT
Cuts at: 0 879 3222
Size: 879 2343
SfcI C'TryA_G
Cuts at: 0 486 545 1117 1384 1818 1863 2397 3222
Size: 486 59 572 267 434 45 534 825
Fragments arranged by size:
825 572 534 486 434 267 59 45
SmaI CCC'GGG
Cuts at: 0 754 3222
Size: 754 2468
SmlI C'TyrA_G
Cuts at: 0 33 247 1317 3222
Size: 33 214 1070 1905
Fragments arranged by size:
1905 1070 214 33
StuI AGG'CCT
Cuts at: 0 307 988 3222
Size: 307 681 2234
Fragments arranged by size:
2234 681 307
StyI C'CwwG_G
Cuts at: 0 551 591 1981 2469 2774 3222
Size: 551 40 1390 488 305 448
Fragments arranged by size:
1390 551 488 448 305 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 358 1120 3222
Size: 77 281 762 2102
Fragments arranged by size:
2102 762 281 77
TatI w'GTAC_w
Cuts at: 0 623 877 3222
Size: 623 254 2345
Fragments arranged by size:
2345 623 254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 2512 2544 3222
Size: 268 32 2212 32 678
Fragments arranged by size:
2212 678 268 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1066 1961 2008 3222
Size: 1066 895 47 1214
Fragments arranged by size:
1214 1066 895 47
XmaI C'CCGG_G
Cuts at: 0 752 3222
Size: 752 2470
ZraI GAC'GTC
Cuts at: 0 2372 3222
Size: 2372 850
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SalI SapI ScaI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AclI AflII AhdI ApaLI AscI AseI AsiSI BaeI
BbeI BmtI BplI BsaBI BsiWI BspEI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KasI MfeI MluI NarI NdeI NheI NotI NruI
PacI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI