Restriction map for
pF1KB0056
(Linear) (Six-base) MAPSORT of: KIBB0056.seq Check: 9562 from: 1 to: 4599
KIBB0056.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1542 1572 2328 3270 4215 4245 4599
Size: 1542 30 756 942 945 30 354
Fragments arranged by size:
1542 945 942 756 354 30 30
AccI GT'mk_AC
Cuts at: 0 4079 4445 4599
Size: 4079 366 154
Fragments arranged by size:
4079 366 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1133 1346 1658 1987 3943 4025 4599
Size: 695 438 213 312 329 1956 82 574
Fragments arranged by size:
1956 695 574 438 329 312 213 82
AgeI A'CCGG_T
Cuts at: 0 2349 4599
Size: 2349 2250
AleI CACnn'nnGTG
Cuts at: 0 1705 4599
Size: 1705 2894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4093 4125 4599
Size: 4093 32 474
Fragments arranged by size:
4093 474 32
AlwNI CAG_nnn'CTG
Cuts at: 0 315 330 1093 1324 1522 1965 2565 3309
Size: 315 15 763 231 198 443 600 744
Cuts at: 3309 3367 3496 3621 3831 3975 4599
Size: 58 129 125 210 144 624
Fragments arranged by size:
763 744 624 600 443 315 231 210
198 144 129 125 58 15
ApaI G_GGCC'C
Cuts at: 0 584 661 836 862 987 1255 1273 1401
Size: 584 77 175 26 125 268 18 128
Cuts at: 1401 2656 2902 4168 4535 4599
Size: 1255 246 1266 367 64
Fragments arranged by size:
1266 1255 584 367 268 246 175 128
125 77 64 26 18
ApoI r'AATT_y
Cuts at: 0 505 606 4599
Size: 505 101 3993
Fragments arranged by size:
3993 505 101
AvaI C'yCGr_G
Cuts at: 0 12 139 385 497 692 863 2264 2768
Size: 12 127 246 112 195 171 1401 504
Cuts at: 2768 3296 4220 4523 4562 4599
Size: 528 924 303 39 37
Fragments arranged by size:
1401 924 528 504 303 246 195 171
127 112 39 37 12
AvrII C'CTAG_G
Cuts at: 0 2662 3247 4599
Size: 2662 585 1352
Fragments arranged by size:
2662 1352 585
BamHI G'GATC_C
Cuts at: 0 2355 4599
Size: 2355 2244
BanI G'GyrC_C
Cuts at: 0 1494 4599
Size: 1494 3105
BanII G_rGCy'C
Cuts at: 0 584 661 836 862 987 1255 1273 1401
Size: 584 77 175 26 125 268 18 128
Cuts at: 1401 2011 2290 2656 2687 2887 2902 3029 3602
Size: 610 279 366 31 200 15 127 573
Cuts at: 3602 4050 4168 4535 4599
Size: 448 118 367 64
Fragments arranged by size:
610 584 573 448 367 366 279 268
200 175 128 127 125 118 77 64
31 26 18 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 2376 2505 4419 4599
Size: 2376 129 1914 180
Fragments arranged by size:
2376 1914 180 129
BbvCI CC'TCA_GC
Cuts at: 0 1064 3792 4599
Size: 1064 2728 807
Fragments arranged by size:
2728 1064 807
BciVI GTATCCnnnnn_n'
Cuts at: 0 773 3635 4481 4599
Size: 773 2862 846 118
Fragments arranged by size:
2862 846 773 118
BclI T'GATC_A
Cuts at: 0 945 4599
Size: 945 3654
BglI GCCn_nnn'nGGC
Cuts at: 0 866 3326 4028 4599
Size: 866 2460 702 571
Fragments arranged by size:
2460 866 702 571
BglII A'GATC_T
Cuts at: 0 3668 4599
Size: 3668 931
BlpI GC'TnA_GC
Cuts at: 0 308 3554 4599
Size: 308 3246 1045
Fragments arranged by size:
3246 1045 308
Bme1580I G_kGCm'C
Cuts at: 0 429 584 661 836 862 987 1255 1273
Size: 429 155 77 175 26 125 268 18
Cuts at: 1273 1401 1497 2033 2344 2656 2902 3587 4168
Size: 128 96 536 311 312 246 685 581
Cuts at: 4168 4535 4599
Size: 367 64
Fragments arranged by size:
685 581 536 429 367 312 311 268
246 175 155 128 125 96 77 64
26 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 717 1275 1756 1828 2020 3397 3530 3531
Size: 717 558 481 72 192 1377 133 1
Cuts at: 3531 3926 4599
Size: 395 673
Fragments arranged by size:
1377 717 673 558 481 395 192 133
72 1
BmtI G_CTAG'C
Cuts at: 0 4259 4599
Size: 4259 340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2489 3621 3904 4599
Size: 2489 1132 283 695
Fragments arranged by size:
2489 1132 695 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 673 1178 1577 1589 1664 2188 2462
Size: 158 515 505 399 12 75 524 274
Cuts at: 2462 3209 3899 4599
Size: 747 690 700
Fragments arranged by size:
747 700 690 524 515 505 399 274
158 75 12
Bpu10I CC'TnA_GC
Cuts at: 0 870 1064 3792 4493 4599
Size: 870 194 2728 701 106
Fragments arranged by size:
2728 870 701 194 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1453 2274 3139 3802 4381 4599
Size: 1000 453 821 865 663 579 218
Fragments arranged by size:
1000 865 821 663 579 453 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 3246 4599
Size: 3246 1353
BsaAI yAC'GTr
Cuts at: 0 74 4599
Size: 74 4525
BsaHI Gr'CG_yC
Cuts at: 0 346 4599
Size: 346 4253
BsaWI w'CCGG_w
Cuts at: 0 2349 4599
Size: 2349 2250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 1539 1569 1662 1692 4093 4123
Size: 578 30 931 30 93 30 2401 30
Cuts at: 4123 4543 4567 4573 4597 4599
Size: 420 24 6 24 2
Fragments arranged by size:
2401 931 578 420 93 30 30 30
30 24 24 6 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1069 1435 1808 1922 2191 2465 2478 2743
Size: 1069 366 373 114 269 274 13 265
Cuts at: 2743 2756 2852 3186 3597 3896 3995 4564 4599
Size: 13 96 334 411 299 99 569 35
Fragments arranged by size:
1069 569 411 373 366 334 299 274
269 265 114 99 96 35 13 13
BseYI C'CCAG_C
Cuts at: 0 375 835 1317 1551 2497 2570 3001 3601
Size: 375 460 482 234 946 73 431 600
Cuts at: 3601 3649 3780 3861 4055 4183 4436 4599
Size: 48 131 81 194 128 253 163
Fragments arranged by size:
946 600 482 460 431 375 253 234
194 163 131 128 81 73 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1571 2585 3724 4599
Size: 1571 1014 1139 875
Fragments arranged by size:
1571 1139 1014 875
BsiEI CG_ry'CG
Cuts at: 0 9 4599
Size: 9 4590
BsiHKAI G_wGCw'C
Cuts at: 0 453 2121 4599
Size: 453 1668 2478
Fragments arranged by size:
2478 1668 453
BsmI GAATG_Cn'
Cuts at: 0 3213 4599
Size: 3213 1386
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4399 4599
Size: 4399 200
Bsp1286I G_dGCh'C
Cuts at: 0 429 453 584 661 836 862 987 1255
Size: 429 24 131 77 175 26 125 268
Cuts at: 1255 1273 1401 1497 2011 2033 2121 2290 2344
Size: 18 128 96 514 22 88 169 54
Cuts at: 2344 2656 2687 2887 2902 3029 3587 3602 4050
Size: 312 31 200 15 127 558 15 448
Cuts at: 4050 4168 4535 4599
Size: 118 367 64
Fragments arranged by size:
558 514 448 429 367 312 268 200
175 169 131 128 127 125 118 96
88 77 64 54 31 26 24 22
18 15 15
BspHI T'CATG_A
Cuts at: 0 684 4599
Size: 684 3915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1542 1572 2328 3270 4215 4245 4599
Size: 1542 30 756 942 945 30 354
Fragments arranged by size:
1542 945 942 756 354 30 30
BsrBI CCG'CTC
Cuts at: 0 307 1395 1425 2814 4506 4599
Size: 307 1088 30 1389 1692 93
Fragments arranged by size:
1692 1389 1088 307 93 30
BsrDI GCAATG_nn'
Cuts at: 0 152 219 4599
Size: 152 67 4380
Fragments arranged by size:
4380 152 67
BsrFI r'CCGG_y
Cuts at: 0 333 2126 2349 2996 3873 4599
Size: 333 1793 223 647 877 726
Fragments arranged by size:
1793 877 726 647 333 223
BssSI C'ACGA_G
Cuts at: 0 3515 4599
Size: 3515 1084
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1456 1522 3309 4599
Size: 330 1126 66 1787 1290
Fragments arranged by size:
1787 1290 1126 330 66
BstBI TT'CG_AA
Cuts at: 0 1751 4599
Size: 1751 2848
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1764 3009 3394 3573 4599
Size: 1764 1245 385 179 1026
Fragments arranged by size:
1764 1245 1026 385 179
BstYI r'GATC_y
Cuts at: 0 95 2355 3668 3972 4599
Size: 95 2260 1313 304 627
Fragments arranged by size:
2260 1313 627 304 95
Bsu36I CC'TnA_GG
Cuts at: 0 2648 2894 2909 3609 3629 4599
Size: 2648 246 15 700 20 970
Fragments arranged by size:
2648 970 700 246 20 15
BtgI C'CryG_G
Cuts at: 0 123 444 738 3008 3683 4073 4599
Size: 123 321 294 2270 675 390 526
Fragments arranged by size:
2270 675 526 390 321 294 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1040 4599
Size: 1040 3559
BtsI GCAGTG_nn'
Cuts at: 0 328 1394 1746 3651 3736 3796 4599
Size: 328 1066 352 1905 85 60 803
Fragments arranged by size:
1905 1066 803 352 328 85 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 223 4599
Size: 223 4376
EaeI y'GGCC_r
Cuts at: 0 3379 4020 4599
Size: 3379 641 579
Fragments arranged by size:
3379 641 579
EarI CTCTTCn'nnn_
Cuts at: 0 986 1412 1433 1634 1802 4599
Size: 986 426 21 201 168 2797
Fragments arranged by size:
2797 986 426 201 168 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 586 776 2506 3709 4599
Size: 586 190 1730 1203 890
Fragments arranged by size:
1730 1203 890 586 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 673 695 1133 1178 1346 1577 1589
Size: 158 515 22 438 45 168 231 12
Cuts at: 1589 1658 1664 1987 2188 2462 3209 3899 3943
Size: 69 6 323 201 274 747 690 44
Cuts at: 3943 4025 4599
Size: 82 574
Fragments arranged by size:
747 690 574 515 438 323 274 231
201 168 158 82 69 45 44 22
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1679 2661 3146 4526 4599
Size: 1679 982 485 1380 73
Fragments arranged by size:
1679 1380 982 485 73
EcoO109I rG'GnC_Cy
Cuts at: 0 657 805 859 983 1210 1251 1252 1269
Size: 657 148 54 124 227 41 1 17
Cuts at: 1269 1270 1300 1653 1792 2103 2652 2653 2666
Size: 1 30 353 139 311 549 1 13
Cuts at: 2666 2898 2905 2940 3118 3172 4165 4244 4336
Size: 232 7 35 178 54 993 79 92
Cuts at: 4336 4370 4531 4532 4559 4599
Size: 34 161 1 27 40
Fragments arranged by size:
993 657 549 353 311 232 227 178
161 148 139 124 92 79 54 54
41 40 35 34 30 27 17 13
7 1 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 2130 4599
Size: 2130 2469
HaeII r_GCGC'y
Cuts at: 0 1446 3621 4599
Size: 1446 2175 978
Fragments arranged by size:
2175 1446 978
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 578 610 1069 1101 2163 2195 3415 3447
Size: 578 32 459 32 1062 32 1220 32
Cuts at: 3447 3547 3579 3964 3996 4543 4575 4599
Size: 100 32 385 32 547 32 24
Fragments arranged by size:
1220 1062 578 547 459 385 100 32
32 32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 0 999 2697 4446 4599
Size: 999 1698 1749 153
Fragments arranged by size:
1749 1698 999 153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 380 535 932 2580 4599
Size: 302 78 155 397 1648 2019
Fragments arranged by size:
2019 1648 397 302 155 78
MscI TGG'CCA
Cuts at: 0 4022 4599
Size: 4022 577
MslI CAynn'nnrTG
Cuts at: 0 458 683 1705 3061 4599
Size: 458 225 1022 1356 1538
Fragments arranged by size:
1538 1356 1022 458 225
MspA1I CmG'CkG
Cuts at: 0 109 315 740 1944 2401 2702 4089 4599
Size: 109 206 425 1204 457 301 1387 510
Fragments arranged by size:
1387 1204 510 457 425 301 206 109
NaeI GCC'GGC
Cuts at: 0 335 2128 3875 4599
Size: 335 1793 1747 724
Fragments arranged by size:
1793 1747 724 335
NcoI C'CATG_G
Cuts at: 0 123 444 3008 3683 4073 4599
Size: 123 321 2564 675 390 526
Fragments arranged by size:
2564 675 526 390 321 123
NgoMIV G'CCGG_C
Cuts at: 0 333 2126 3873 4599
Size: 333 1793 1747 726
Fragments arranged by size:
1793 1747 726 333
NheI G'CTAG_C
Cuts at: 0 4255 4599
Size: 4255 344
NspI r_CATG'y
Cuts at: 0 1434 4599
Size: 1434 3165
PasI CC'CwG_GG
Cuts at: 0 801 1276 2058 3724 4599
Size: 801 475 782 1666 875
Fragments arranged by size:
1666 875 801 782 475
PflMI CCAn_nnn'nTGG
Cuts at: 0 1294 3709 3831 4599
Size: 1294 2415 122 768
Fragments arranged by size:
2415 1294 768 122
PfoI T'CCnGG_A
Cuts at: 0 3593 4301 4599
Size: 3593 708 298
Fragments arranged by size:
3593 708 298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4093 4125 4599
Size: 4093 32 474
Fragments arranged by size:
4093 474 32
PpuMI rG'GwC_Cy
Cuts at: 0 1210 1300 1792 3118 3172 4336 4370 4559
Size: 1210 90 492 1326 54 1164 34 189
Cuts at: 4559 4599
Size: 40
Fragments arranged by size:
1326 1210 1164 492 189 90 54 40
34
PspOMI G'GGCC_C
Cuts at: 0 580 657 832 858 983 1251 1269 1397
Size: 580 77 175 26 125 268 18 128
Cuts at: 1397 2652 2898 4164 4531 4599
Size: 1255 246 1266 367 68
Fragments arranged by size:
1266 1255 580 367 268 246 175 128
125 77 68 26 18
PstI C_TGCA'G
Cuts at: 0 134 521 1569 3127 4013 4178 4599
Size: 134 387 1048 1558 886 165 421
Fragments arranged by size:
1558 1048 886 421 387 165 134
PvuI CG_AT'CG
Cuts at: 0 9 4599
Size: 9 4590
PvuII CAG'CTG
Cuts at: 0 4089 4599
Size: 4089 510
SacII CC_GC'GG
Cuts at: 0 741 4599
Size: 741 3858
SalI G'TCGA_C
Cuts at: 0 4444 4599
Size: 4444 155
SanDI GG'GwC_CC
Cuts at: 0 3172 4559 4599
Size: 3172 1387 40
Fragments arranged by size:
3172 1387 40
SapI GCTCTTCn'nnn_
Cuts at: 0 1433 1634 4599
Size: 1433 201 2965
Fragments arranged by size:
2965 1433 201
SbfI CC_TGCA'GG
Cuts at: 0 521 4599
Size: 521 4078
SexAI A'CCwGG_T
Cuts at: 0 3393 4599
Size: 3393 1206
SfcI C'TryA_G
Cuts at: 0 130 517 703 1565 2839 3123 3854 4009
Size: 130 387 186 862 1274 284 731 155
Cuts at: 4009 4174 4416 4599
Size: 165 242 183
Fragments arranged by size:
1274 862 731 387 284 242 186 183
165 155 130
SgrAI Cr'CCGG_yG
Cuts at: 0 2349 4599
Size: 2349 2250
SmaI CCC'GGG
Cuts at: 0 694 3298 4599
Size: 694 2604 1301
Fragments arranged by size:
2604 1301 694
SmlI C'TyrA_G
Cuts at: 0 497 1015 1468 2289 3154 3817 4396 4523
Size: 497 518 453 821 865 663 579 127
Cuts at: 4523 4599
Size: 76
Fragments arranged by size:
865 821 663 579 518 497 453 127
76
SnaBI TAC'GTA
Cuts at: 0 74 4599
Size: 74 4525
SpeI A'CTAG_T
Cuts at: 0 231 3418 4599
Size: 231 3187 1181
Fragments arranged by size:
3187 1181 231
SphI G_CATG'C
Cuts at: 0 1434 4599
Size: 1434 3165
StuI AGG'CCT
Cuts at: 0 3186 4462 4599
Size: 3186 1276 137
Fragments arranged by size:
3186 1276 137
StyI C'CwwG_G
Cuts at: 0 26 123 444 548 1853 2099 2617 2662
Size: 26 97 321 104 1305 246 518 45
Cuts at: 2662 3008 3016 3247 3683 4073 4329 4498 4599
Size: 346 8 231 436 390 256 169 101
Fragments arranged by size:
1305 518 436 390 346 321 256 246
231 169 104 101 97 45 26 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 2151 2746 3368 4450 4599
Size: 457 1694 595 622 1082 149
Fragments arranged by size:
1694 1082 622 595 457 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 710 742 1063 1095 1553 1585 3890 3922
Size: 710 32 321 32 458 32 2305 32
Cuts at: 3922 4599
Size: 677
Fragments arranged by size:
2305 710 677 458 321 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3395 4599
Size: 3395 1204
XbaI T'CTAG_A
Cuts at: 0 2220 4599
Size: 2220 2379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 451 1090 2366 4599
Size: 451 639 1276 2233
Fragments arranged by size:
2233 1276 639 451
XhoI C'TCGA_G
Cuts at: 0 497 4523 4599
Size: 497 4026 76
Fragments arranged by size:
4026 497 76
XmaI C'CCGG_G
Cuts at: 0 692 3296 4599
Size: 692 2604 1303
Fragments arranged by size:
2604 1303 692
XmnI GAAnn'nnTTC
Cuts at: 0 1811 4599
Size: 1811 2788
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AloI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI SexAI SfcI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
ApaLI AscI AseI AsiSI BaeI BbeI BcgI BfrBI
BmgBI BsaBI BsiWI BspEI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI RsrII SacI
ScaI SfiI SfoI SgfI SrfI SspI SwaI TatI
ZraI