Restriction map for pF1KB0031
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BglI        BlnI        BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        ClaI        CpoI        
DraI        DraII       DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AclI         2580  AACGTT               AA^CGTT
AflII         964  CTTAAG               C^TTAAG
AflIII       1906  ACACGT               A^C[GA][CT]GT
AhdI         1474  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1089  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         1069  ATTAAT               AT^TAAT
AsnI         1069  ATTAAT               AT^TAAT
AspEI        1474  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI           9  GAGCTC               G[AT]GC[AT]^C
AvaI         2507  CTCGAG               C^[CT]CG[GA]G
AvaII         532  GGTCC                G^G[AT]CC
BanII           9  GAGCTC               G[GA]GC[CT]^C
BbvCI         275  CCTCAGC              CCTCAGC(-5/-2)
BfrI          964  CTTAAG               C^TTAAG
BglII        1404  AGATCT               A^GATCT
BlpI         1492  GCTCAGC              GC^T[ACGT]AGC
BmyI            9  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I     1492  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1913  CACGTA               [CT]AC^GT[GA]
BsiHKAI         9  GAGCTC               G[AT]GC[AT]^C
BsoBI        2507  CTCGAG               C^[CT]CG[GA]G
Bsp1286I        9  GAGCTC               G[AGT]GC[ACT]^C
BsrFI        2175  GCCGGT               [GA]^CCGG[CT]
BstBI        1655  TTCGAA               TT^CGAA
BstEII       2178  GGTGACC              G^GT[ACGT]ACC
BstPI        2178  GGTGACC              G^GT[ACGT]ACC
CelII        1492  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2175  GCCGGT               [GA]^CCGG[CT]
Cfr13I        532  GGTCC                G^G[ACGT]CC
DraIII        130  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1212  CCGTGG               C^C[GA][CT]GG
Eam1105I     1474  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2178  GGTGACC              G^GT[ACGT]ACC
EcoRV        2086  GATATC               GAT^ATC
FauI         2586  GCGGG                CCCGC(4/6)
HaeIII       2174  GGCC                 GG^CC
HapII        2176  CCGG                 C^CGG
HincII       2162  GTCAAC               GT[CT]^[GA]AC
HindII       2162  GTCAAC               GT[CT]^[GA]AC
HpaII        2176  CCGG                 C^CGG
MspI         2176  CCGG                 C^CGG
MwoI          472  GCCAATGTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          779  ACATGC               [GA]CATG^[CT]
NspV         1655  TTCGAA               TT^CGAA
PaeR7I       2507  CTCGAG               C^TCGAG
PshBI        1069  ATTAAT               AT^TAAT
Psp1406I     2580  AACGTT               AA^CGTT
SacI            9  GAGCTC               GAGCT^C
Sau96I        532  GGTCC                G^G[ACGT]CC
SfuI         1655  TTCGAA               TT^CGAA
XbaI         1950  TCTAGA               T^CTAGA
XcmI         2102  CCAAAGATCATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2507  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BsaBI         955  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2276  GATCTCCATC          
BseRI          12  CTCCTC               GAGGAG(10/8)
             2659  GAGGAG              
BsgI         2333  CTGCAC               GTGCAG(16/14)
              761  GTGCAG              
BsmI         1320  GAATGC               GAATGC(1/-1)
             2625  GAATGC              
BspHI         489  TCATGA               T^CATGA
              957  TCATGA              
EcoRI          65  GAATTC               G^AATTC
             1652  GAATTC              
MamI          955  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2276  GATCTCCATC          
NheI         1111  GCTAGC               G^CTAGC
             2254  GCTAGC              
NlaIV         111  GGTTCC               GG[ACGT]^[ACGT]CC
              407  GGTTCC              
PflMI         129  CCACGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1504  CCACAAGCTGG         
PleI         1474  GACTC                GAGTC(4/5)
             1480  GAGTC               
PstI           82  CTGCAG               CTGCA^G
             2414  CTGCAG              
RcaI          489  TCATGA               T^CATGA
              957  TCATGA              
SspI           57  AATATT               AAT^ATT
             1250  AATATT              
TaqI         1656  TCGA                 T^CGA
             2508  TCGA                
TthHB8I      1656  TCGA                 T^CGA
             2508  TCGA                
Van91I        129  CCACGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1504  CCACAAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     BbsI        BbvI        BfaI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
NdeII       NlaIII      PvuII       RsaI        SapI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com