Restriction map for pF1KB0031
 (Linear) (Six-base) MAPSORT of: KIBB0031.seq  Check: 3042  from: 1  to: 2745



KIBB0031.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AclI AA'CG_TT

Cuts at:      0    2581    2745 
   Size:       2581     164
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1429    1711    2119    2745 
   Size:        925     504     282     408     626

  Fragments arranged by size: 

                 925     626     504     408     282
 
AflII C'TTAA_G

Cuts at:      0     964    2745 
   Size:        964    1781
 
AflIII A'CryG_T

Cuts at:      0    1906    2745 
   Size:       1906     839
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1479    2745 
   Size:       1479    1266
 
AleI CACnn'nnGTG

Cuts at:      0     559    2745 
   Size:        559    2186
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2599    2745 
   Size:       2599     146
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     311     343    2745 
   Size:        311      32    2402

  Fragments arranged by size: 

                2402     311      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1094    2745 
   Size:       1094    1651
 
ApoI r'AATT_y

Cuts at:      0      65     388     497     510     969    1652    1878    1891 
   Size:         65     323     109      13     459     683     226      13

Cuts at:   1891    2590    2745 
   Size:        699     155

  Fragments arranged by size: 

                 699     683     459     323     226     155     109      65
                  13      13
 
AseI AT'TA_AT

Cuts at:      0    1070    2745 
   Size:       1070    1675
 
AvaI C'yCGr_G

Cuts at:      0    2507    2745 
   Size:       2507     238
 
BanII G_rGCy'C

Cuts at:      0      13    2745 
   Size:         13    2732
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     534     572    2068    2137    2423    2745 
   Size:        534      38    1496      69     286     322

  Fragments arranged by size: 

                1496     534     322     286      69      38
 
BbvCI CC'TCA_GC

Cuts at:      0     276    2745 
   Size:        276    2469
 
BglII A'GATC_T

Cuts at:      0    1404    2745 
   Size:       1404    1341
 
BlpI GC'TnA_GC

Cuts at:      0    1493    2745 
   Size:       1493    1252
 
BmgBI CAC'GTC

Cuts at:      0    1909    2745 
   Size:       1909     836
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1170    2745 
   Size:       1170    1575
 
BmtI G_CTAG'C

Cuts at:      0    1115    2258    2745 
   Size:       1115    1143     487

  Fragments arranged by size: 

                1143    1115     487
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1505    1535    2745 
   Size:        184    1321      30    1210

  Fragments arranged by size: 

                1321    1210     184      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     276    2695    2745 
   Size:        276    2419      50

  Fragments arranged by size: 

                2419     276      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     350    1258    2677    2745 
   Size:         45     305     908    1419      68

  Fragments arranged by size: 

                1419     908     305      68      45
 
BsaAI yAC'GTr

Cuts at:      0    1915    2745 
   Size:       1915     830
 
BsaBI GATnn'nnATC

Cuts at:      0     959    2280    2745 
   Size:        959    1321     465

  Fragments arranged by size: 

                1321     959     465
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52     230     260    1880    1910    2745 
   Size:         22      30     178      30    1620      30     835

  Fragments arranged by size: 

                1620     835     178      30      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3    2674    2745 
   Size:          3    2671      71

  Fragments arranged by size: 

                2671      71       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    2318    2745 
   Size:        782    1536     427

  Fragments arranged by size: 

                1536     782     427
 
BsiHKAI G_wGCw'C

Cuts at:      0      13    2745 
   Size:         13    2732
 
BsmI GAATG_Cn'

Cuts at:      0    1326    2631    2745 
   Size:       1326    1305     114

  Fragments arranged by size: 

                1326    1305     114
 
Bsp1286I G_dGCh'C

Cuts at:      0      13    2745 
   Size:         13    2732
 
BspHI T'CATG_A

Cuts at:      0     489     957    2745 
   Size:        489     468    1788

  Fragments arranged by size: 

                1788     489     468
 
BsrFI r'CCGG_y

Cuts at:      0    2175    2745 
   Size:       2175     570
 
BstBI TT'CG_AA

Cuts at:      0    1656    2745 
   Size:       1656    1089
 
BstEII G'GTnAC_C

Cuts at:      0    2178    2745 
   Size:       2178     567
 
BstYI r'GATC_y

Cuts at:      0    1033    1404    2275    2487    2745 
   Size:       1033     371     871     212     258

  Fragments arranged by size: 

                1033     871     371     258     212
 
BtgI C'CryG_G

Cuts at:      0    1212    2745 
   Size:       1212    1533
 
BtsI GCAGTG_nn'

Cuts at:      0    1212    2745 
   Size:       1212    1533
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1088    1123    2745 
   Size:       1088      35    1622

  Fragments arranged by size: 

                1622    1088      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     135    2745 
   Size:        135    2610
 
EarI CTCTTCn'nnn_

Cuts at:      0       1     236     638     707     749    1433    1532    1950 
   Size:          1     235     402      69      42     684      99     418

Cuts at:   1950    1961    2114    2138    2745 
   Size:         11     153      24     607

  Fragments arranged by size: 

                 684     607     418     402     235     153      99      69
                  42      24      11       1
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     115    2745 
   Size:        115    2630
 
EcoICRI GAG'CTC

Cuts at:      0      11    2745 
   Size:         11    2734
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     925    1429    1505    1535    1711    2119    2745 
   Size:        184     741     504      76      30     176     408     626

  Fragments arranged by size: 

                 741     626     504     408     184     176      76      30
            
 
EcoRI G'AATT_C

Cuts at:      0      65    1652    2745 
   Size:         65    1587    1093

  Fragments arranged by size: 

                1587    1093      65
 
EcoRV GAT'ATC

Cuts at:      0    2088    2745 
   Size:       2088     657
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2044    2149    2710    2745 
   Size:       2044     105     561      35

  Fragments arranged by size: 

                2044     561     105      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     643     675    1261    1293    1465    1497 
   Size:        259      32     352      32     586      32     172      32

Cuts at:   1497    1612    1644    2091    2123    2243    2275    2745 
   Size:        115      32     447      32     120      32     470

  Fragments arranged by size: 

                 586     470     447     352     259     172     120     115
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2164    2745 
   Size:       2164     581
 
HindIII A'AGCT_T

Cuts at:      0    1935    2126    2692    2745 
   Size:       1935     191     566      53

  Fragments arranged by size: 

                1935     566     191      53
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1598    2745 
   Size:       1598    1147
 
MslI CAynn'nnrTG

Cuts at:      0     559    1022    1961    2745 
   Size:        559     463     939     784

  Fragments arranged by size: 

                 939     784     559     463
 
MspA1I CmG'CkG

Cuts at:      0     236     916    1497    1713    2745 
   Size:        236     680     581     216    1032

  Fragments arranged by size: 

                1032     680     581     236     216
 
NheI G'CTAG_C

Cuts at:      0    1111    2254    2745 
   Size:       1111    1143     491

  Fragments arranged by size: 

                1143    1111     491
 
NspI r_CATG'y

Cuts at:      0     783    2745 
   Size:        783    1962
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     135    1510    2745 
   Size:        135    1375    1235

  Fragments arranged by size: 

                1375    1235     135
 
PfoI T'CCnGG_A

Cuts at:      0     818    2745 
   Size:        818    1927
 
PsiI TTA'TAA

Cuts at:      0     374    2716    2745 
   Size:        374    2342      29

  Fragments arranged by size: 

                2342     374      29
 
PstI C_TGCA'G

Cuts at:      0      86    2418    2745 
   Size:         86    2332     327

  Fragments arranged by size: 

                2332     327      86
 
PvuII CAG'CTG

Cuts at:      0     916    1497    1713    2745 
   Size:        916     581     216    1032

  Fragments arranged by size: 

                1032     916     581     216
 
SacI G_AGCT'C

Cuts at:      0      13    2745 
   Size:         13    2732
 
SapI GCTCTTCn'nnn_

Cuts at:      0       1    1433    1950    2745 
   Size:          1    1432     517     795

  Fragments arranged by size: 

                1432     795     517       1
 
SfcI C'TryA_G

Cuts at:      0      82     884    1181    1788    2414    2745 
   Size:         82     802     297     607     626     331

  Fragments arranged by size: 

                 802     626     607     331     297      82
 
SmlI C'TyrA_G

Cuts at:      0      24     365     964    1237    2507    2656    2745 
   Size:         24     341     599     273    1270     149      89

  Fragments arranged by size: 

                1270     599     341     273     149      89      24
 
SspI AAT'ATT

Cuts at:      0      59    1252    2745 
   Size:         59    1193    1493

  Fragments arranged by size: 

                1493    1193      59
 
StyI C'CwwG_G

Cuts at:      0    1532    1985    2183    2271    2745 
   Size:       1532     453     198      88     474

  Fragments arranged by size: 

                1532     474     453     198      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     865    2745 
   Size:        865    1880
 
TatI w'GTAC_w

Cuts at:      0    2418    2663    2672    2745 
   Size:       2418     245       9      73

  Fragments arranged by size: 

                2418     245      73       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     230     262    1366    1398    2162    2194    2745 
   Size:        230      32    1104      32     764      32     551

  Fragments arranged by size: 

                1104     764     551     230      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1950    2745 
   Size:       1950     795
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2109    2745 
   Size:       2109     636
 
XhoI C'TCGA_G

Cuts at:      0    2507    2745 
   Size:       2507     238
 
XmnI GAAnn'nnTTC

Cuts at:      0     746    1013    2036    2745 
   Size:        746     267    1023     709

  Fragments arranged by size: 

                1023     746     709     267
 

 Enzymes that do cut:

     AclI     AcuI    AflII   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI    BanII     BbsI    BbvCI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrFI    BstBI   BstEII    BstYI     BtgI     BtsI    CspCI   DraIII
     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     NheI     NspI    PflMI
     PfoI     PsiI     PstI    PvuII     SacI     SapI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI     AgeI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI Bme1580I     BplI     BsaI    BsaHI
    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI     DrdI     EaeI     EagI    EcoNI EcoO109I
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com