Restriction map for pF1KA1825
 (Linear) (Six-base) MAPSORT of: KIAA1825.seq  Check: 8872  from: 1  to: 3612



KIAA1825.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     394    2462    2493    3612 
   Size:        394    2068      31    1119

  Fragments arranged by size: 

                2068    1119     394      31
 
AccI GT'mk_AC

Cuts at:      0     908    1040    3209    3308    3612 
   Size:        908     132    2169      99     304

  Fragments arranged by size: 

                2169     908     304     132      99
 
Acc65I G'GTAC_C

Cuts at:      0    1396    3612 
   Size:       1396    2216
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1228    1909    2575    2635    3612 
   Size:       1228     681     666      60     977

  Fragments arranged by size: 

                1228     977     681     666      60
 
AfeI AGC'GCT

Cuts at:      0    1867    3612 
   Size:       1867    1745
 
AflIII A'CryG_T

Cuts at:      0     860    1535    3612 
   Size:        860     675    2077

  Fragments arranged by size: 

                2077     860     675
 
AleI CACnn'nnGTG

Cuts at:      0    1165    1753    2941    3612 
   Size:       1165     588    1188     671

  Fragments arranged by size: 

                1188    1165     671     588
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      46     295    1294    2389    2392    3612 
   Size:         46     249     999    1095       3    1220

  Fragments arranged by size: 

                1220    1095     999     249      46       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0     250    1851    2280    2478    2811    3063    3528    3612 
   Size:        250    1601     429     198     333     252     465      84

  Fragments arranged by size: 

                1601     465     429     333     252     250     198      84
            
 
ApaI G_GGCC'C

Cuts at:      0      45    1710    1968    3612 
   Size:         45    1665     258    1644

  Fragments arranged by size: 

                1665    1644     258      45
 
ApaLI G'TGCA_C

Cuts at:      0     364    1440    3190    3612 
   Size:        364    1076    1750     422

  Fragments arranged by size: 

                1750    1076     422     364
 
ApoI r'AATT_y

Cuts at:      0     517     661    1856    3612 
   Size:        517     144    1195    1756

  Fragments arranged by size: 

                1756    1195     517     144
 
AvaI C'yCGr_G

Cuts at:      0      82     348    2036    2102    2387    2687    2821    3122 
   Size:         82     266    1688      66     285     300     134     301

Cuts at:   3122    3241    3385    3612 
   Size:        119     144     227

  Fragments arranged by size: 

                1688     301     300     285     266     227     144     134
                 119      82      66
 
BamHI G'GATC_C

Cuts at:      0    1559    3612 
   Size:       1559    2053
 
BanI G'GyrC_C

Cuts at:      0      30     188     238     432     690     770     999    1159 
   Size:         30     158      50     194     258      80     229     160

Cuts at:   1159    1396    1747    2593    2608    3612 
   Size:        237     351     846      15    1004

  Fragments arranged by size: 

                1004     846     351     258     237     229     194     160
                 158      80      50      30      15
 
BanII G_rGCy'C

Cuts at:      0      45     464     767    1088    1553    1710    1930    1968 
   Size:         45     419     303     321     465     157     220      38

Cuts at:   1968    2049    2395    2759    3241    3612 
   Size:         81     346     364     482     371

  Fragments arranged by size: 

                 482     465     419     371     364     346     321     303
                 220     157      81      45      38
 
BbeI G_GCGC'C

Cuts at:      0     192    2612    3612 
   Size:        192    2420    1000

  Fragments arranged by size: 

                2420    1000     192
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     497     638    1100    3612 
   Size:        497     141     462    2512

  Fragments arranged by size: 

                2512     497     462     141
 
BbvCI CC'TCA_GC

Cuts at:      0    1099    3612 
   Size:       1099    2513
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1013    1047    3612 
   Size:       1013      34    2565

  Fragments arranged by size: 

                2565    1013      34
 
BclI T'GATC_A

Cuts at:      0    2234    2918    3612 
   Size:       2234     684     694

  Fragments arranged by size: 

                2234     694     684
 
BglI GCCn_nnn'nGGC

Cuts at:      0      69     118     168    2435    2518    3479    3612 
   Size:         69      49      50    2267      83     961     133

  Fragments arranged by size: 

                2267     961     133      83      69      50      49
 
BglII A'GATC_T

Cuts at:      0    2030    3612 
   Size:       2030    1582
 
Bme1580I G_kGCm'C

Cuts at:      0      35      45     368    1162    1444    1710    1887    1968 
   Size:         35      10     323     794     282     266     177      81

Cuts at:   1968    2002    3194    3612 
   Size:         34    1192     418

  Fragments arranged by size: 

                1192     794     418     323     282     266     177      81
                  35      34      10
 
BmgBI CAC'GTC

Cuts at:      0    1011    1146    2604    2628    3612 
   Size:       1011     135    1458      24     984

  Fragments arranged by size: 

                1458    1011     984     135      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1669    1989    2860    3612 
   Size:       1669     320     871     752

  Fragments arranged by size: 

                1669     871     752     320
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2370    3612 
   Size:       2370    1242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1108    1345    1456    2146    2434    2715    3040 
   Size:        571     537     237     111     690     288     281     325

Cuts at:   3040    3163    3612 
   Size:        123     449

  Fragments arranged by size: 

                 690     571     537     449     325     288     281     237
                 123     111
 
Bpu10I CC'TnA_GC

Cuts at:      0    1099    1472    2657    2704    2800    3199    3612 
   Size:       1099     373    1185      47      96     399     413

  Fragments arranged by size: 

                1185    1099     413     399     373      96      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    2122    2158    3094    3612 
   Size:        520    1602      36     936     518

  Fragments arranged by size: 

                1602     936     520     518      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1321    2166    2810    3612 
   Size:       1321     845     644     802

  Fragments arranged by size: 

                1321     845     802     644
 
BsaAI yAC'GTr

Cuts at:      0    2268    2571    2916    3612 
   Size:       2268     303     345     696

  Fragments arranged by size: 

                2268     696     345     303
 
BsaHI Gr'CG_yC

Cuts at:      0     189    2609    3612 
   Size:        189    2420    1003

  Fragments arranged by size: 

                2420    1003     189
 
BsaWI w'CCGG_w

Cuts at:      0     872    1249    1556    3612 
   Size:        872     377     307    2056

  Fragments arranged by size: 

                2056     872     377     307
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     436     466     952     982    2036    2066    3612 
   Size:        436      30     486      30    1054      30    1546

  Fragments arranged by size: 

                1546    1054     486     436      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2785    3436    3444    3612 
   Size:       2785     651       8     168

  Fragments arranged by size: 

                2785     651     168       8
 
BseYI C'CCAG_C

Cuts at:      0     257     263     406    1408    1923    2061    2076    2471 
   Size:        257       6     143    1002     515     138      15     395

Cuts at:   2471    2562    3612 
   Size:         91    1050

  Fragments arranged by size: 

                1050    1002     515     395     257     143     138      91
                  15       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     372     457     862    1126    1192    1527    1963 
   Size:        301      71      85     405     264      66     335     436

Cuts at:   1963    2151    2266    2521    2919    3612 
   Size:        188     115     255     398     693

  Fragments arranged by size: 

                 693     436     405     398     335     301     264     255
                 188     115      85      71      66
 
BsiEI CG_ry'CG

Cuts at:      0     978    1463    1790    1955    2752    3564    3612 
   Size:        978     485     327     165     797     812      48

  Fragments arranged by size: 

                 978     812     797     485     327     165      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     368     464    1444    3194    3612 
   Size:        368      96     980    1750     418

  Fragments arranged by size: 

                1750     980     418     368      96
 
BsmI GAATG_Cn'

Cuts at:      0    2217    3612 
   Size:       2217    1395
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     972    1649    3612 
   Size:        972     677    1963

  Fragments arranged by size: 

                1963     972     677
 
Bsp1286I G_dGCh'C

Cuts at:      0      35      45     368     464     767    1088    1162    1444 
   Size:         35      10     323      96     303     321      74     282

Cuts at:   1444    1553    1710    1887    1930    1968    2002    2049    2395 
   Size:        109     157     177      43      38      34      47     346

Cuts at:   2395    2759    3194    3241    3612 
   Size:        364     435      47     371

  Fragments arranged by size: 

                 435     371     364     346     323     321     303     282
                 177     157     109      96      74      47      47      43
                  38      35      34      10
 
BspEI T'CCGG_A

Cuts at:      0     872    1556    3612 
   Size:        872     684    2056

  Fragments arranged by size: 

                2056     872     684
 
BspHI T'CATG_A

Cuts at:      0    2231    3612 
   Size:       2231    1381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     394    2462    2493    3612 
   Size:        394    2068      31    1119

  Fragments arranged by size: 

                2068    1119     394      31
 
BsrBI CCG'CTC

Cuts at:      0     980    2172    2253    2661    2673    3612 
   Size:        980    1192      81     408      12     939

  Fragments arranged by size: 

                1192     980     939     408      81      12
 
BsrDI GCAATG_nn'

Cuts at:      0     372     486     932    3515    3612 
   Size:        372     114     446    2583      97

  Fragments arranged by size: 

                2583     446     372     114      97
 
BsrFI r'CCGG_y

Cuts at:      0     117     167    1212    2336    3073    3612 
   Size:        117      50    1045    1124     737     539

  Fragments arranged by size: 

                1124    1045     737     539     117      50
 
BsrGI T'GTAC_A

Cuts at:      0    3155    3269    3612 
   Size:       3155     114     343

  Fragments arranged by size: 

                3155     343     114
 
BssHII G'CGCG_C

Cuts at:      0      96      98     377    3612 
   Size:         96       2     279    3235

  Fragments arranged by size: 

                3235     279      96       2
 
BssSI C'ACGA_G

Cuts at:      0     304     463    1716    1753    3484    3612 
   Size:        304     159    1253      37    1731     128

  Fragments arranged by size: 

                1731    1253     304     159     128      37
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     852    3612 
   Size:        852    2760
 
BstBI TT'CG_AA

Cuts at:      0     515    3612 
   Size:        515    3097
 
BstEII G'GTnAC_C

Cuts at:      0    1668    1759    2943    3612 
   Size:       1668      91    1184     669

  Fragments arranged by size: 

                1668    1184     669      91
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     819    2604    3333    3612 
   Size:        819    1785     729     279

  Fragments arranged by size: 

                1785     819     729     279
 
BstYI r'GATC_y

Cuts at:      0     284     641     869    1559    2030    2204    2966    3612 
   Size:        284     357     228     690     471     174     762     646

  Fragments arranged by size: 

                 762     690     646     471     357     284     228     174
            
 
BtgI C'CryG_G

Cuts at:      0     455     572    1199    1790    3320    3612 
   Size:        455     117     627     591    1530     292

  Fragments arranged by size: 

                1530     627     591     455     292     117
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1350    1500    1932    2850    3612 
   Size:       1350     150     432     918     762

  Fragments arranged by size: 

                1350     918     762     432     150
 
BtsI GCAGTG_nn'

Cuts at:      0    2255    2347    3612 
   Size:       2255      92    1265

  Fragments arranged by size: 

                2255    1265      92
 
DraIII CAC_nnn'GTG

Cuts at:      0    2226    3612 
   Size:       2226    1386
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1677    3612 
   Size:       1677    1935
 
EaeI y'GGCC_r

Cuts at:      0     160     975    1496    1523    1643    1787    1952    2645 
   Size:        160     815     521      27     120     144     165     693

Cuts at:   2645    3071    3317    3323    3335    3428    3557    3612 
   Size:        426     246       6      12      93     129      55

  Fragments arranged by size: 

                 815     693     521     426     246     165     160     144
                 129     120      93      55      27      12       6
 
EagI C'GGCC_G

Cuts at:      0     975    1787    1952    3612 
   Size:        975     812     165    1660

  Fragments arranged by size: 

                1660     975     812     165
 
EarI CTCTTCn'nnn_

Cuts at:      0    1297    1351    3091    3612 
   Size:       1297      54    1740     521

  Fragments arranged by size: 

                1740    1297     521      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2473    3612 
   Size:       2473    1139
 
EcoICRI GAG'CTC

Cuts at:      0     462    3612 
   Size:        462    3150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1108    1228    1345    1456    1909    2146    2434 
   Size:        571     537     120     117     111     453     237     288

Cuts at:   2434    2575    2635    2715    3040    3163    3612 
   Size:        141      60      80     325     123     449

  Fragments arranged by size: 

                 571     537     453     449     325     288     237     141
                 123     120     117     111      80      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2787    2803    3612 
   Size:       2787      16     809

  Fragments arranged by size: 

                2787     809      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41    1404    1707    1964    1965    2121    3525    3587 
   Size:         41    1363     303     257       1     156    1404      62

Cuts at:   3587    3612 
   Size:         25

  Fragments arranged by size: 

                1404    1363     303     257     156      62      41      25
                   1
 
EcoRI G'AATT_C

Cuts at:      0     517    3612 
   Size:        517    3095
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     514    3612 
   Size:        514    3098
 
FspI TGC'GCA

Cuts at:      0    1282    3612 
   Size:       1282    2330
 
HaeII r_GCGC'y

Cuts at:      0     124     181     192    1869    2062    2355    2612    2641 
   Size:        124      57      11    1677     193     293     257      29

Cuts at:   2641    2977    3553    3612 
   Size:        336     576      59

  Fragments arranged by size: 

                1677     576     336     293     257     193     124      59
                  57      29      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525     952     984    2036    2068    3612 
   Size:        493      32     427      32    1052      32    1544

  Fragments arranged by size: 

                1544    1052     493     427      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1218    1503    1613    3297    3612 
   Size:       1218     285     110    1684     315

  Fragments arranged by size: 

                1684    1218     315     285     110
 
KasI G'GCGC_C

Cuts at:      0     188    2608    3612 
   Size:        188    2420    1004

  Fragments arranged by size: 

                2420    1004     188
 
KpnI G_GTAC'C

Cuts at:      0    1400    3612 
   Size:       1400    2212
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     845    3612 
   Size:        845    2767
 
MscI TGG'CCA

Cuts at:      0    1525    2647    3319    3325    3337    3430    3612 
   Size:       1525    1122     672       6      12      93     182

  Fragments arranged by size: 

                1525    1122     672     182      93      12       6
 
MslI CAynn'nnrTG

Cuts at:      0     479     817    1165    1753    2941    3172    3319    3325 
   Size:        479     338     348     588    1188     231     147       6

Cuts at:   3325    3331    3496    3612 
   Size:          6     165     116

  Fragments arranged by size: 

                1188     588     479     348     338     231     165     147
                 116       6       6
 
MspA1I CmG'CkG

Cuts at:      0      10     250     652    1031    1223    1375    1737    2478 
   Size:         10     240     402     379     192     152     362     741

Cuts at:   2478    2748    2933    3612 
   Size:        270     185     679

  Fragments arranged by size: 

                 741     679     402     379     362     270     240     192
                 185     152      10
 
NaeI GCC'GGC

Cuts at:      0     119    2338    3075    3612 
   Size:        119    2219     737     537

  Fragments arranged by size: 

                2219     737     537     119
 
NarI GG'CG_CC

Cuts at:      0     189    2609    3612 
   Size:        189    2420    1003

  Fragments arranged by size: 

                2420    1003     189
 
NcoI C'CATG_G

Cuts at:      0    3320    3612 
   Size:       3320     292
 
NgoMIV G'CCGG_C

Cuts at:      0     117    2336    3073    3612 
   Size:        117    2219     737     539

  Fragments arranged by size: 

                2219     737     539     117
 
NspI r_CATG'y

Cuts at:      0     864    1539    2264    2623    3612 
   Size:        864     675     725     359     989

  Fragments arranged by size: 

                 989     864     725     675     359
 
PasI CC'CwG_GG

Cuts at:      0    1266    3612 
   Size:       1266    2346
 
PciI A'CATG_T

Cuts at:      0     860    1535    3612 
   Size:        860     675    2077

  Fragments arranged by size: 

                2077     860     675
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2226    3612 
   Size:       2226    1386
 
PfoI T'CCnGG_A

Cuts at:      0     626    2198    2731    3533    3612 
   Size:        626    1572     533     802      79

  Fragments arranged by size: 

                1572     802     626     533      79
 
PmlI CAC'GTG

Cuts at:      0    2268    2916    3612 
   Size:       2268     648     696

  Fragments arranged by size: 

                2268     696     648
 
PpuMI rG'GwC_Cy

Cuts at:      0    1404    3525    3587    3612 
   Size:       1404    2121      62      25

  Fragments arranged by size: 

                2121    1404      62      25
 
PspOMI G'GGCC_C

Cuts at:      0      41    1706    1964    3612 
   Size:         41    1665     258    1648

  Fragments arranged by size: 

                1665    1648     258      41
 
PspXI vC'TCGA_Gb

Cuts at:      0    2821    3612 
   Size:       2821     791
 
PstI C_TGCA'G

Cuts at:      0    1374    2318    2459    3575    3612 
   Size:       1374     944     141    1116      37

  Fragments arranged by size: 

                1374    1116     944     141      37
 
PvuII CAG'CTG

Cuts at:      0     652    1375    1737    2478    3612 
   Size:        652     723     362     741    1134

  Fragments arranged by size: 

                1134     741     723     652     362
 
RsrII CG'GwC_CG

Cuts at:      0    1247    3612 
   Size:       1247    2365
 
SacI G_AGCT'C

Cuts at:      0     464    3612 
   Size:        464    3148
 
SanDI GG'GwC_CC

Cuts at:      0    3587    3612 
   Size:       3587      25
 
SbfI CC_TGCA'GG

Cuts at:      0    2459    3612 
   Size:       2459    1153
 
ScaI AGT'ACT

Cuts at:      0     784    3612 
   Size:        784    2828
 
SexAI A'CCwGG_T

Cuts at:      0     995    3612 
   Size:        995    2617
 
SfcI C'TryA_G

Cuts at:      0     792    1370    1691    2314    2455    2953    2997    3055 
   Size:        792     578     321     623     141     498      44      58

Cuts at:   3055    3571    3612 
   Size:        516      41

  Fragments arranged by size: 

                 792     623     578     516     498     321     141      58
                  44      41
 
SfoI GGC'GCC

Cuts at:      0     190    2610    3612 
   Size:        190    2420    1002

  Fragments arranged by size: 

                2420    1002     190
 
SmaI CCC'GGG

Cuts at:      0      84    2038    2104    2389    2689    3612 
   Size:         84    1954      66     285     300     923

  Fragments arranged by size: 

                1954     923     300     285      84      66
 
SmlI C'TyrA_G

Cuts at:      0     499    2137    2173    2821    3109    3612 
   Size:        499    1638      36     648     288     503

  Fragments arranged by size: 

                1638     648     503     499     288      36
 
SphI G_CATG'C

Cuts at:      0    2264    2623    3612 
   Size:       2264     359     989

  Fragments arranged by size: 

                2264     989     359
 
SrfI GCCC'GGGC

Cuts at:      0      84    3612 
   Size:         84    3528
 
StuI AGG'CCT

Cuts at:      0     832    3612 
   Size:        832    2780
 
StyI C'CwwG_G

Cuts at:      0    1163    1400    2186    3320    3612 
   Size:       1163     237     786    1134     292

  Fragments arranged by size: 

                1163    1134     786     292     237
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     267    1449    1747    2620    3612 
   Size:        226      41    1182     298     873     992

  Fragments arranged by size: 

                1182     992     873     298     226      41
 
TatI w'GTAC_w

Cuts at:      0     782    1538    3155    3269    3612 
   Size:        782     756    1617     114     343

  Fragments arranged by size: 

                1617     782     756     343     114
 
Tth111I GACn'n_nGTC

Cuts at:      0     964    3565    3612 
   Size:        964    2601      47

  Fragments arranged by size: 

                2601     964      47
 
XbaI T'CTAG_A

Cuts at:      0    1767    3612 
   Size:       1767    1845
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     462    1623    2847    3612 
   Size:        462    1161    1224     765

  Fragments arranged by size: 

                1224    1161     765     462
 
XhoI C'TCGA_G

Cuts at:      0    2821    3612 
   Size:       2821     791
 
XmaI C'CCGG_G

Cuts at:      0      82    2036    2102    2387    2687    3612 
   Size:         82    1954      66     285     300     925

  Fragments arranged by size: 

                1954     925     300     285      82      66
 
XmnI GAAnn'nnTTC

Cuts at:      0    1822    3612 
   Size:       1822    1790
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI
    PvuII    RsrII     SacI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI    BfrBI     BlpI     BmtI    BsaBI
    BsiWI  BstZ17I   Bsu36I     ClaI    CspCI     DraI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI     PsrI
     PvuI    SacII     SalI     SapI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SspI     SwaI     TstI     ZraI
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