Restriction map for
pF1KA1793
(Circular) (Six-base) MAPSORT of: pF1KA1793.seq Check: 4278 from: 1 to: 6575
pF1KA1793 6575 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2163 2593 2163
Size: 430 6145
AatII G_ACGT'C
Cuts at: 943 3565 943
Size: 2622 3953
AccI GT'mk_AC
Cuts at: 3613 3613
Size: 6575
Acc65I G'GTAC_C
Cuts at: 3591 3591
Size: 6575
AclI AA'CG_TT
Cuts at: 6415 6415
Size: 6575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 1046 1228 1276 1534 2657 2800 4400 4832
Size: 102 182 48 258 1123 143 1600 432
Cuts at: 4832 5579 5847 944
Size: 747 268 1672
Fragments arranged by size:
1672 1600 1123 747 432 268 258 182
143 102 48
AfeI AGC'GCT
Cuts at: 2650 2650
Size: 6575
AflII C'TTAA_G
Cuts at: 514 514
Size: 6575
AflIII A'CryG_T
Cuts at: 1405 1607 3927 5031 6008 1405
Size: 202 2320 1104 977 1972
Fragments arranged by size:
2320 1972 1104 977 202
AgeI A'CCGG_T
Cuts at: 4963 4963
Size: 6575
AlwNI CAG_nnn'CTG
Cuts at: 303 564 792 1220 1380 1443 2169 2609 3313
Size: 261 228 428 160 63 726 440 704
Cuts at: 3313 3880 5447 303
Size: 567 1567 1431
Fragments arranged by size:
1567 1431 726 704 567 440 428 261
228 160 63
ApaI G_GGCC'C
Cuts at: 834 834
Size: 6575
ApaLI G'TGCA_C
Cuts at: 5345 5345
Size: 6575
ApoI r'AATT_y
Cuts at: 3579 5928 6184 3579
Size: 2349 256 3970
Fragments arranged by size:
3970 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
AvaI C'yCGr_G
Cuts at: 332 3041 3595 332
Size: 2709 554 3312
Fragments arranged by size:
3312 2709 554
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2387 2420 2387
Size: 33 6542
BamHI G'GATC_C
Cuts at: 3600 3600
Size: 6575
BanI G'GyrC_C
Cuts at: 3591 4005 4253 4288 3591
Size: 414 248 35 5878
Fragments arranged by size:
5878 414 248 35
BanII G_rGCy'C
Cuts at: 297 383 730 834 1142 1271 1304 1664 1808
Size: 86 347 104 308 129 33 360 144
Cuts at: 1808 2210 2999 3589 4619 297
Size: 402 789 590 1030 2253
Fragments arranged by size:
2253 1030 789 590 402 360 347 308
144 129 104 86 33
BbeI G_GCGC'C
Cuts at: 4009 4257 4009
Size: 248 6327
BbsI GAAGACnn'nnnn_
Cuts at: 662 1940 2645 3389 662
Size: 1278 705 744 3848
Fragments arranged by size:
3848 1278 744 705
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2608 2642 90
Size: 34 2484 34 4023
Fragments arranged by size:
4023 2484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1485 3114 4473 5234 6575 1485
Size: 1629 1359 761 1341 1485
Fragments arranged by size:
1629 1485 1359 1341 761
BclI T'GATC_A
Cuts at: 2678 4095 2678
Size: 1417 5158
BfrBI ATG'CAT
Cuts at: 1038 1038
Size: 6575
BglI GCCn_nnn'nGGC
Cuts at: 415 2194 2500 415
Size: 1779 306 4490
Fragments arranged by size:
4490 1779 306
BglII A'GATC_T
Cuts at: 873 4090 6127 873
Size: 3217 2037 1321
Fragments arranged by size:
3217 2037 1321
BlpI GC'TnA_GC
Cuts at: 1544 2630 3299 3687 1544
Size: 1086 669 388 4432
Fragments arranged by size:
4432 1086 669 388
Bme1580I G_kGCm'C
Cuts at: 834 4200 4293 5349 834
Size: 3366 93 1056 2060
Fragments arranged by size:
3366 2060 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3787 3868 4203 6347 3787
Size: 81 335 2144 4015
Fragments arranged by size:
4015 2144 335 81
BmtI G_CTAG'C
Cuts at: 403 2924 403
Size: 2521 4054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 378 714 4942 378
Size: 336 4228 2011
Fragments arranged by size:
4228 2011 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 343 406 898 1372 1612 1642 1669 1831
Size: 42 63 492 474 240 30 27 162
Cuts at: 1831 2986 3178 301
Size: 1155 192 3698
Fragments arranged by size:
3698 1155 492 474 240 192 162 63
42 30 27
Bpu10I CC'TnA_GC
Cuts at: 296 3007 296
Size: 2711 3864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1648 1888 2350 2452 3160 3751 4630 5122
Size: 522 240 462 102 708 591 879 492
Cuts at: 5122 5420 1126
Size: 298 2281
Fragments arranged by size:
2281 879 708 591 522 492 462 298
240 102
BsaI GGTCTCn'nnnn_
Cuts at: 322 757 3136 6279 322
Size: 435 2379 3143 618
Fragments arranged by size:
3143 2379 618 435
BsaAI yAC'GTr
Cuts at: 3928 3928
Size: 6575
BsaBI GATnn'nnATC
Cuts at: 6 691 4089 6126 6
Size: 685 3398 2037 455
Fragments arranged by size:
3398 2037 685 455
BsaHI Gr'CG_yC
Cuts at: 940 2186 3407 3562 4006 4254 4956 6452 940
Size: 1246 1221 155 444 248 702 1496 1063
Fragments arranged by size:
1496 1246 1221 1063 702 444 248 155
BsaWI w'CCGG_w
Cuts at: 739 1683 3764 4285 4963 5237 5384 5795 739
Size: 944 2081 521 678 274 147 411 1519
Fragments arranged by size:
2081 1519 944 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 365 395 1129 1159 1195 1225 1348 1378 1444
Size: 30 734 30 36 30 123 30 66
Cuts at: 1444 1474 365
Size: 30 5466
Fragments arranged by size:
5466 734 123 66 36 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 187 298 340 490 1126 1129 1216 1465
Size: 21 111 42 150 636 3 87 249
Cuts at: 1465 1836 1849 2254 3007 3229 3244 3253 3385
Size: 371 13 405 753 222 15 9 132
Cuts at: 3385 3409 3412 166
Size: 24 3 3329
Fragments arranged by size:
3329 753 636 405 371 249 222 150
132 111 87 42 24 21 15 13
9 3 3
BseYI C'CCAG_C
Cuts at: 138 410 2365 4000 4758 5335 5831 138
Size: 272 1955 1635 758 577 496 882
Fragments arranged by size:
1955 1635 882 758 577 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 728 1366 1846 2505 2595 2716 2974 229
Size: 499 638 480 659 90 121 258 3830
Fragments arranged by size:
3830 659 638 499 480 258 121 90
BsiEI CG_ry'CG
Cuts at: 104 4117 4163 5371 104
Size: 4013 46 1208 1308
Fragments arranged by size:
4013 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 730 1142 1152 1271 1304 1664 1808 2210 2999
Size: 412 10 119 33 360 144 402 789
Cuts at: 2999 3589 4367 4557 5349 730
Size: 590 778 190 792 1956
Fragments arranged by size:
1956 792 789 778 590 412 402 360
190 144 119 33 10
BsmI GAATG_Cn'
Cuts at: 668 2833 3057 3081 668
Size: 2165 224 24 4162
Fragments arranged by size:
4162 2165 224 24
BsmBI CGTCTCn'nnnn_
Cuts at: 1528 3553 1528
Size: 2025 4550
Bsp1286I G_dGCh'C
Cuts at: 297 383 730 834 1142 1152 1271 1304 1664
Size: 86 347 104 308 10 119 33 360
Cuts at: 1664 1808 2210 2999 3589 4200 4293 4367 4557
Size: 144 402 789 590 611 93 74 190
Cuts at: 4557 4619 5349 297
Size: 62 730 1523
Fragments arranged by size:
1523 789 730 611 590 402 360 347
308 190 144 119 104 93 86 74
62 33 10
BspEI T'CCGG_A
Cuts at: 1683 5795 1683
Size: 4112 2463
BspHI T'CATG_A
Cuts at: 1319 5751 1319
Size: 4432 2143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2163 2593 2634 3625 3839 4141 4522 2163
Size: 430 41 991 214 302 381 4216
Fragments arranged by size:
4216 991 430 381 302 214 41
BsrBI CCG'CTC
Cuts at: 50 1596 2244 4867 4921 6405 6570 50
Size: 1546 648 2623 54 1484 165 55
Fragments arranged by size:
2623 1546 1484 648 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1524 2376 4487 1524
Size: 852 2111 3612
Fragments arranged by size:
3612 2111 852
BsrFI r'CCGG_y
Cuts at: 1367 2188 4573 4754 4963 1367
Size: 821 2385 181 209 2979
Fragments arranged by size:
2979 2385 821 209 181
BssSI C'ACGA_G
Cuts at: 4846 5204 4846
Size: 358 6217
BstAPI GCAn_nnn'nTGC
Cuts at: 792 1380 1443 2169 2694 2828 3952 792
Size: 588 63 726 525 134 1124 3415
Fragments arranged by size:
3415 1124 726 588 525 134 63
BstBI TT'CG_AA
Cuts at: 4936 4936
Size: 6575
BstEII G'GTnAC_C
Cuts at: 3102 3102
Size: 6575
BstYI r'GATC_y
Cuts at: 1 437 554 627 873 2030 3309 3600 4067
Size: 436 117 73 246 1157 1279 291 467
Cuts at: 4067 4090 4425 4671 5683 5769 5781 6127 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1279 1157 1012 467 449 436 346 335
291 246 246 117 86 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 1486 3314 1486
Size: 1828 4747
BtgI C'CryG_G
Cuts at: 2843 2843
Size: 6575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1081 4525 4706 4869 1081
Size: 3444 181 163 2787
Fragments arranged by size:
3444 2787 181 163
BtsI GCAGTG_nn'
Cuts at: 228 1465 1720 1804 1873 1879 2986 3283 3818
Size: 1237 255 84 69 6 1107 297 535
Cuts at: 3818 228
Size: 2985
Fragments arranged by size:
2985 1237 1107 535 297 255 84 69
6
DraI TTT'AAA
Cuts at: 3574 3574
Size: 6575
DrdI GACnn_nn'nnGTC
Cuts at: 4281 5139 4281
Size: 858 5717
EaeI y'GGCC_r
Cuts at: 2762 4160 4334 4725 4752 6097 2762
Size: 1398 174 391 27 1345 3240
Fragments arranged by size:
3240 1398 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4160 4160
Size: 6575
EarI CTCTTCn'nnn_
Cuts at: 152 158 164 176 718 1022 1076 1850 2033
Size: 6 6 12 542 304 54 774 183
Cuts at: 2033 2242 2338 2506 2548 2587 2780 3010 3347
Size: 209 96 168 42 39 193 230 337
Cuts at: 3347 3362 3386 3420 4598 4808 152
Size: 15 24 34 1178 210 1919
Fragments arranged by size:
1919 1178 774 542 337 304 230 210
209 193 183 168 96 54 42 39
34 24 15 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2989 4783 5093 5239 6385 2989
Size: 1794 310 146 1146 3179
Fragments arranged by size:
3179 1794 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 1140 1269 1302 1662 1806 2208 2997 3587
Size: 412 129 33 360 144 402 789 590
Cuts at: 3587 728
Size: 3716
Fragments arranged by size:
3716 789 590 412 402 360 144 129
33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 343 406 898 944 1046 1228 1276 1372
Size: 42 63 492 46 102 182 48 96
Cuts at: 1372 1534 1612 1642 1669 1831 2657 2800 2986
Size: 162 78 30 27 162 826 143 186
Cuts at: 2986 3178 4400 4832 5579 5847 301
Size: 192 1222 432 747 268 1029
Fragments arranged by size:
1222 1029 826 747 492 432 268 192
186 182 162 162 143 102 96 78
63 48 46 42 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 622 817 2802 3512 622
Size: 195 1985 710 3685
Fragments arranged by size:
3685 1985 710 195
EcoO109I rG'GnC_Cy
Cuts at: 831 1629 2166 3714 831
Size: 798 537 1548 3692
Fragments arranged by size:
3692 1548 798 537
EcoRI G'AATT_C
Cuts at: 3579 3579
Size: 6575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4594 4594
Size: 6575
FspI TGC'GCA
Cuts at: 4356 4356
Size: 6575
HaeII r_GCGC'y
Cuts at: 410 2652 4009 4257 5279 6258 410
Size: 2242 1357 248 1022 979 727
Fragments arranged by size:
2242 1357 1022 979 727 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 546 578 598 630 1195 1227 1527
Size: 32 266 32 20 32 565 32 300
Cuts at: 1527 1559 3397 3429 3550 3582 4417 4447 4449
Size: 32 1838 32 121 32 835 30 2
Cuts at: 4449 4479 248
Size: 30 2344
Fragments arranged by size:
2344 1838 835 565 300 266 121 32
32 32 32 32 32 32 30 30
20 2
HincII GTy'rAC
Cuts at: 243 3614 243
Size: 3371 3204
KasI G'GCGC_C
Cuts at: 4005 4253 4005
Size: 248 6327
KpnI G_GTAC'C
Cuts at: 3595 3595
Size: 6575
MluI A'CGCG_T
Cuts at: 6008 6008
Size: 6575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1058 2060 5246 5430 1058
Size: 1002 3186 184 2203
Fragments arranged by size:
3186 2203 1002 184
MscI TGG'CCA
Cuts at: 2764 4336 2764
Size: 1572 5003
MslI CAynn'nnrTG
Cuts at: 356 602 1610 4564 4691 356
Size: 246 1008 2954 127 2240
Fragments arranged by size:
2954 2240 1008 246 127
MspA1I CmG'CkG
Cuts at: 309 1284 1443 1566 1620 1647 1785 1848 1953
Size: 975 159 123 54 27 138 63 105
Cuts at: 1953 2292 2609 2736 2907 2964 2976 3686 4000
Size: 339 317 127 171 57 12 710 314
Cuts at: 4000 4360 5373 5618 309
Size: 360 1013 245 1266
Fragments arranged by size:
1266 1013 975 710 360 339 317 314
245 171 159 138 127 123 105 63
57 54 27 12
NaeI GCC'GGC
Cuts at: 1369 4756 1369
Size: 3387 3188
NarI GG'CG_CC
Cuts at: 4006 4254 4006
Size: 248 6327
NcoI C'CATG_G
Cuts at: 2843 2843
Size: 6575
NgoMIV G'CCGG_C
Cuts at: 1367 4754 1367
Size: 3387 3188
NheI G'CTAG_C
Cuts at: 399 2920 399
Size: 2521 4054
NsiI A_TGCA'T
Cuts at: 1040 1040
Size: 6575
NspI r_CATG'y
Cuts at: 1409 1611 1971 3628 4128 5035 1409
Size: 202 360 1657 500 907 2949
Fragments arranged by size:
2949 1657 907 500 360 202
PciI A'CATG_T
Cuts at: 1405 1607 5031 1405
Size: 202 3424 2949
Fragments arranged by size:
3424 2949 202
PflMI CCAn_nnn'nTGG
Cuts at: 1891 1891
Size: 6575
PfoI T'CCnGG_A
Cuts at: 383 1808 2069 383
Size: 1425 261 4889
Fragments arranged by size:
4889 1425 261
PmeI GTTT'AAAC
Cuts at: 3574 3574
Size: 6575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5714 5746 5714
Size: 32 6543
PpuMI rG'GwC_Cy
Cuts at: 1629 1629
Size: 6575
PshAI GACnn'nnGTC
Cuts at: 2189 2189
Size: 6575
PspOMI G'GGCC_C
Cuts at: 830 830
Size: 6575
PstI C_TGCA'G
Cuts at: 308 623 788 839 920 1133 1232 1376 1448
Size: 315 165 51 81 213 99 144 72
Cuts at: 1448 1460 1481 1790 1853 1952 2174 2258 2690
Size: 12 21 309 63 99 222 84 432
Cuts at: 2690 2885 2906 2981 3622 4307 308
Size: 195 21 75 641 685 2576
Fragments arranged by size:
2576 685 641 432 315 309 222 213
195 165 144 99 99 84 81 75
72 63 51 21 21 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6575
PvuII CAG'CTG
Cuts at: 309 1284 1443 1566 1620 1647 1785 1848 1953
Size: 975 159 123 54 27 138 63 105
Cuts at: 1953 2292 2609 2736 2907 2964 2976 4000 4360
Size: 339 317 127 171 57 12 1024 360
Cuts at: 4360 309
Size: 2524
Fragments arranged by size:
2524 1024 975 360 339 317 171 159
138 127 123 105 63 57 54 27
12
RsrII CG'GwC_CG
Cuts at: 4770 4770
Size: 6575
SacI G_AGCT'C
Cuts at: 730 1142 1271 1304 1664 1808 2210 2999 3589
Size: 412 129 33 360 144 402 789 590
Cuts at: 3589 730
Size: 3716
Fragments arranged by size:
3716 789 590 412 402 360 144 129
33
SalI G'TCGA_C
Cuts at: 3612 3612
Size: 6575
SapI GCTCTTCn'nnn_
Cuts at: 718 1022 1076 1850 2033 2338 2780 4598 4808
Size: 304 54 774 183 305 442 1818 210
Cuts at: 4808 718
Size: 2485
Fragments arranged by size:
2485 1818 774 442 305 304 210 183
54
SbfI CC_TGCA'GG
Cuts at: 623 839 2174 3622 623
Size: 216 1335 1448 3576
Fragments arranged by size:
3576 1448 1335 216
ScaI AGT'ACT
Cuts at: 6092 6092
Size: 6575
SfcI C'TryA_G
Cuts at: 33 304 373 619 784 835 916 1087 1129
Size: 271 69 246 165 51 81 171 42
Cuts at: 1129 1228 1372 1444 1456 1477 1786 1849 1948
Size: 99 144 72 12 21 309 63 99
Cuts at: 1948 2170 2254 2686 2881 2902 2977 3618 4303
Size: 222 84 432 195 21 75 641 685
Cuts at: 4303 5296 5487 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 641 432 309 271 246
222 195 191 171 165 144 99 99
84 81 75 72 69 63 51 42
21 21 12
SfoI GGC'GCC
Cuts at: 4007 4255 4007
Size: 248 6327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
SmaI CCC'GGG
Cuts at: 334 3043 3597 334
Size: 2709 554 3312
Fragments arranged by size:
3312 2709 554
SmlI C'TyrA_G
Cuts at: 514 1141 1663 1903 2329 2467 3139 3730 4645
Size: 627 522 240 426 138 672 591 915
Cuts at: 4645 5137 5399 514
Size: 492 262 1690
Fragments arranged by size:
1690 915 672 627 591 522 492 426
262 240 138
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6575
SphI G_CATG'C
Cuts at: 1971 3628 4128 1971
Size: 1657 500 4418
Fragments arranged by size:
4418 1657 500
StuI AGG'CCT
Cuts at: 1669 2503 3021 3518 1669
Size: 834 518 497 4726
Fragments arranged by size:
4726 834 518 497
StyI C'CwwG_G
Cuts at: 365 450 1962 2843 3709 4063 365
Size: 85 1512 881 866 354 2877
Fragments arranged by size:
2877 1512 881 866 354 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1207 2458 4158 4291 4750 4959 1207
Size: 1251 1700 133 459 209 2823
Fragments arranged by size:
2823 1700 1251 459 209 133
TatI w'GTAC_w
Cuts at: 1754 3170 6090 1754
Size: 1416 2920 2239
Fragments arranged by size:
2920 2239 1416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1129 1161 1348 1380 1444 1476 6092 6124 1129
Size: 32 187 32 64 32 4616 32 1580
Fragments arranged by size:
4616 1580 187 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3564 4372 3564
Size: 808 5767
XbaI T'CTAG_A
Cuts at: 3606 3606
Size: 6575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2148 3153 4070 114
Size: 2034 1005 917 2619
Fragments arranged by size:
2619 2034 1005 917
XmaI C'CCGG_G
Cuts at: 332 3041 3595 332
Size: 2709 554 3312
Fragments arranged by size:
3312 2709 554
XmnI GAAnn'nnTTC
Cuts at: 668 668
Size: 6575
ZraI GAC'GTC
Cuts at: 941 3563 941
Size: 2622 3953
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AleI AlfI AloI AscI AvrII BbvCI BmgBI
BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI PacI PasI PmlI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI