Restriction map for pF1KA1558
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# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AviII       AvrII       BbrPI       BcgI        BclI        
BfrI        BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstBI       BstEII      BstPI       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MroI        MspA1I      MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII        1662  GACGTC               GACGT^C
AccII        2550  CGCG                 CG^CG
Alw44I       1405  GTGCAC               G^TGCAC
AocI         1508  CCTTAGG              CC^T[ACGT]AGG
ApaI         1422  GGGCCC               GGGCC^C
ApaLI        1405  GTGCAC               G^TGCAC
AseI         1296  ATTAAT               AT^TAAT
AsnI         1296  ATTAAT               AT^TAAT
AspI         1468  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1008  GGATCC               G^GATCC
BanI         2553  GGCGCC               G^G[CT][GA]CC
BbeI         2553  GGCGCC               GGCGC^C
BbvCI        1345  GCTGAGG              CCTCAGC(-5/-2)
BciVI         228  GTATCC               GTATCC(6/5)
BcnI         1028  CCCGG                CC^[GC]GG
BglI          869  GCCCACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2403  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2403  GCTCAGC              GC^T[ACGT]AGC
BsaI          668  GAGACC               GGTCTC(1/5)
BsmI         1885  GCATTC               GAATGC(1/-1)
BspHI         948  TCATGA               T^CATGA
BspLU11I     1130  ACATGT               A^CATGT
BspMI        1552  ACCTGC               ACCTGC(4/8)
BsrDI        1727  GCAATG               GCAATG(2/0)
Bst1107I     1143  GTATAC               GTA^TAC
BstAPI        537  GCAAGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        2550  CGCG                 CG^CG
BstXI        2038  CCACCCAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1143  GTATAC               GTA^TAC
Bsu36I       1508  CCTTAGG              CC^T[ACGT]AGG
CelII        2403  GCTCAGC              GC^T[ACGT]AGC
DraI         1591  TTTAAA               TTT^AAA
Eco81I       1508  CCTTAGG              CC^T[ACGT]AGG
EcoRV        1050  GATATC               GAT^ATC
EcoT22I       629  ATGCAT               ATGCA^T
HaeII        2553  GGCGCC               [GA]GCGC^[CT]
HapII        1029  CCGG                 C^CGG
HgaI         2512  GACGC                GACGC(5/10)
HpaII        1029  CCGG                 C^CGG
KasI         2553  GGCGCC               G^GCGCC
MspI         1029  CCGG                 C^CGG
MvnI         2550  CGCG                 CG^CG
NarI         2553  GGCGCC               GG^CGCC
NciI         1028  CCCGG                CC^[GC]GG
NsiI          629  ATGCAT               ATGCA^T
PflFI        1468  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1091  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2433  GACTGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1296  ATTAAT               AT^TAAT
PstI         2534  CTGCAG               CTGCA^G
RcaI          948  TCATGA               T^CATGA
SexAI        2607  ACCAGGT              A^CC[AT]GGT
SfoI         2553  GGCGCC               GGC^GCC
StuI         2560  AGGCCT               AGG^CCT
Tsp45I       2612  GTGAC                ^GT[GC]AC
Tth111I      1468  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI         1662  GACGTC               G[GA]^CG[CT]C
             2553  GGCGCC              
AflIII        511  ACGTGT               A^C[GA][CT]GT
             1130  ACATGT              
AlwNI        1946  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2429  CAGAGACTG           
BalI         2312  TGGCCA               TGG^CCA
             2592  TGGCCA              
BanII         700  GAGCCC               G[GA]GC[CT]^C
             1422  GGGCCC              
BsaHI        1662  GACGTC               G[GA]^CG[CT]C
             2553  GGCGCC              
BsmBI        1489  GAGACG               CGTCTC(1/5)
             2149  GAGACG              
DraIII        641  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2606  CACCAGGTG           
EaeI         2312  TGGCCA               [CT]^GGCC[GA]
             2592  TGGCCA              
EcoT14I      2249  CCATGG               C^C[AT][AT]GG
             2315  CCATGG              
Hin1I        1662  GACGTC               G[GA]^CG[CT]C
             2553  GGCGCC              
HphI         1255  GGTGA                GGTGA(8/7)
             2611  GGTGA               
MluNI        2312  TGGCCA               TGG^CCA
             2592  TGGCCA              
MscI         2312  TGGCCA               TGG^CCA
             2592  TGGCCA              
MslI          622  CATTCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2616  CACTTCAGTG          
NcoI         2249  CCATGG               C^CATGG
             2315  CCATGG              
NspI         1130  ACATGT               [GA]CATG^[CT]
             1807  GCATGT              
SfcI         2414  CTGTAG               C^T[GA][CT]AG
             2534  CTGCAG              
SspI          760  AATATT               AAT^ATT
             1789  AATATT              
StyI         2249  CCATGG               C^C[AT][AT]GG
             2315  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BbsI        BbvI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       XmnI        
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