Restriction map for pF1KA1520
 (Circular) (Six-base) MAPSORT of: pF1KA1520.seq  Check: 6153  from: 1  to: 5411



pF1KA1520  5411 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1287    1287 
   Size:       5411
 
AccI GT'mk_AC

Cuts at:   1502    2449    1502 
   Size:        947    4464
 
Acc65I G'GTAC_C

Cuts at:   2427    2427 
   Size:       5411
 
AclI AA'CG_TT

Cuts at:   5251    5251 
   Size:       5411
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    171    1671    2326    3236    3668    4415    4683     171 
   Size:       1500     655     910     432     747     268     899

  Fragments arranged by size: 

                1500     910     899     747     655     432     268
 
AfeI AGC'GCT

Cuts at:   2121    2121 
   Size:       5411
 
AflIII A'CryG_T

Cuts at:   1334    2763    3867    4844    1334 
   Size:       1429    1104     977    1901

  Fragments arranged by size: 

                1901    1429    1104     977
 
AgeI A'CCGG_T

Cuts at:   3799    3799 
   Size:       5411
 
AleI CACnn'nnGTG

Cuts at:    370    1054     370 
   Size:        684    4727
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    301     301 
   Size:       5411
 
AlwNI CAG_nnn'CTG

Cuts at:    546     636    1671    2286    2289    2334    2716    4283     546 
   Size:         90    1035     615       3      45     382    1567    1674

  Fragments arranged by size: 

                1674    1567    1035     615     382      90      45       3
            
 
ApaI G_GGCC'C

Cuts at:    335     462    1727     335 
   Size:        127    1265    4019

  Fragments arranged by size: 

                4019    1265     127
 
ApaLI G'TGCA_C

Cuts at:   1576    4181    1576 
   Size:       2605    2806
 
ApoI r'AATT_y

Cuts at:   2415    4764    5020    2415 
   Size:       2349     256    2806

  Fragments arranged by size: 

                2806    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5411
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5411
 
AvaI C'yCGr_G

Cuts at:    604    1728    2069    2431     604 
   Size:       1124     341     362    3584

  Fragments arranged by size: 

                3584    1124     362     341
 
BamHI G'GATC_C

Cuts at:   2436    2436 
   Size:       5411
 
BanI G'GyrC_C

Cuts at:    535    2026    2272    2427    2841    3089    3124     535 
   Size:       1491     246     155     414     248      35    2822

  Fragments arranged by size: 

                2822    1491     414     248     246     155      35
 
BanII G_rGCy'C

Cuts at:    335     462    1361    1498    1727    1747    1874    2076    2425 
   Size:        127     899     137     229      20     127     202     349

Cuts at:   2425    3455     335 
   Size:       1030    2291

  Fragments arranged by size: 

                2291    1030     899     349     229     202     137     127
                 127      20
 
BbeI G_GCGC'C

Cuts at:   2030    2845    3093    2030 
   Size:        815     248    4348

  Fragments arranged by size: 

                4348     815     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    177    1017    1056    1240     177 
   Size:        840      39     184    4348

  Fragments arranged by size: 

                4348     840     184      39
 
BbvCI CC'TCA_GC

Cuts at:    572     572 
   Size:       5411
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     774     808    1425    1459    1557    1591      90 
   Size:         34     650      34     617      34      98      34    3910

  Fragments arranged by size: 

                3910     650     617      98      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3309    4070    5411    3309 
   Size:        761    1341    3309

  Fragments arranged by size: 

                3309    1341     761
 
BclI T'GATC_A

Cuts at:   2931    2931 
   Size:       5411
 
BglI GCCn_nnn'nGGC

Cuts at:    339     568     339 
   Size:        229    5182
 
BglII A'GATC_T

Cuts at:   2926    4963    2926 
   Size:       2037    3374
 
BlpI GC'TnA_GC

Cuts at:   2209    2523    2209 
   Size:        314    5097
 
Bme1580I G_kGCm'C

Cuts at:    335     462    1580    1727    2275    3036    3129    4185     335 
   Size:        127    1118     147     548     761      93    1056    1561

  Fragments arranged by size: 

                1561    1118    1056     761     548     147     127      93
            
 
BmgBI CAC'GTC

Cuts at:    660     660 
   Size:       5411
 
BmrI ACTGGGnnnn_n'

Cuts at:   2086    2623    2704    3039    5183    2086 
   Size:        537      81     335    2144    2314

  Fragments arranged by size: 

                2314    2144     537     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3778    3778 
   Size:       5411
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     568    1627    1783    1801    1975     232 
   Size:        336    1059     156      18     174    3668

  Fragments arranged by size: 

                3668    1059     336     174     156      18
 
Bpu10I CC'TnA_GC

Cuts at:    572     572 
   Size:       5411
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2587    3466    3958    4256    2587 
   Size:        879     492     298    3742

  Fragments arranged by size: 

                3742     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    706    1011    1210    5115     706 
   Size:        305     199    3905    1002

  Fragments arranged by size: 

                3905    1002     305     199
 
BsaAI yAC'GTr

Cuts at:   2764    2764 
   Size:       5411
 
BsaBI GATnn'nnATC

Cuts at:      6    2925    4962       6 
   Size:       2919    2037     455

  Fragments arranged by size: 

                2919    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    613    2027    2842    3090    3792    5288     613 
   Size:       1414     815     248     702    1496     736

  Fragments arranged by size: 

                1496    1414     815     736     702     248
 
BsaWI w'CCGG_w

Cuts at:   1241    2600    3121    3799    4073    4220    4631    1241 
   Size:       1359     521     678     274     147     411    2021

  Fragments arranged by size: 

                2021    1359     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    949     979     991    1021     949 
   Size:         30      12      30    5339

  Fragments arranged by size: 

                5339      30      30      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1228    1273    1276    1279    1228 
   Size:         45       3       3    5360

  Fragments arranged by size: 

                5360      45       3       3
 
BseYI C'CCAG_C

Cuts at:    291     327    2030    2334    2836    3594    4171    4667     291 
   Size:         36    1703     304     502     758     577     496    1035

  Fragments arranged by size: 

                1703    1035     758     577     502     496     304      36
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2284    2341    2284 
   Size:         57    5354
 
BsiEI CG_ry'CG

Cuts at:    104     594    1560    2953    2999    4207     104 
   Size:        490     966    1393      46    1208    1308

  Fragments arranged by size: 

                1393    1308    1208     966     490      46
 
BsiHKAI G_wGCw'C

Cuts at:   1580    1747    2215    2225    2425    3203    3393    4185    1580 
   Size:        167     468      10     200     778     190     792    2806

  Fragments arranged by size: 

                2806     792     778     468     200     190     167      10
            
 
BsmI GAATG_Cn'

Cuts at:   1188    1188 
   Size:       5411
 
Bsp1286I G_dGCh'C

Cuts at:    335     462    1361    1498    1580    1727    1747    1874    2076 
   Size:        127     899     137      82     147      20     127     202

Cuts at:   2076    2215    2225    2275    2425    3036    3129    3203    3393 
   Size:        139      10      50     150     611      93      74     190

Cuts at:   3393    3455    4185     335 
   Size:         62     730    1561

  Fragments arranged by size: 

                1561     899     730     611     202     190     150     147
                 139     137     127     127      93      82      74      62
                  50      20      10
 
BspEI T'CCGG_A

Cuts at:   1241    4631    1241 
   Size:       3390    2021
 
BspHI T'CATG_A

Cuts at:    146    1124    4587     146 
   Size:        978    3463     970

  Fragments arranged by size: 

                3463     978     970
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    826    1287    1289    2180    2461    2675    2977    3358     826 
   Size:        461       2     891     281     214     302     381    2879

  Fragments arranged by size: 

                2879     891     461     381     302     281     214       2
            
 
BsrBI CCG'CTC

Cuts at:     50     590     908    1889    3703    3757    5241    5406      50 
   Size:        540     318     981    1814      54    1484     165      55

  Fragments arranged by size: 

                1814    1484     981     540     318     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   3323    3323 
   Size:       5411
 
BsrFI r'CCGG_y

Cuts at:    593    1559    2204    3409    3590    3799     593 
   Size:        966     645    1205     181     209    2205

  Fragments arranged by size: 

                2205    1205     966     645     209     181
 
BsrGI T'GTAC_A

Cuts at:   1301    1301 
   Size:       5411
 
BssSI C'ACGA_G

Cuts at:    351     370     670    3682    4040     351 
   Size:         19     300    3012     358    1722

  Fragments arranged by size: 

                3012    1722     358     300      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:    636    2788     636 
   Size:       2152    3259
 
BstBI TT'CG_AA

Cuts at:    891    3772     891 
   Size:       2881    2530
 
BstEII G'GTnAC_C

Cuts at:   1098    1098 
   Size:       5411
 
BstXI CCAn_nnnn'nTGG

Cuts at:    309    2124     309 
   Size:       1815    3596
 
BstYI r'GATC_y

Cuts at:      1     252    2436    2903    2926    3261    3507    4519    4605 
   Size:        251    2184     467      23     335     246    1012      86

Cuts at:   4605    4617    4963       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2184    1012     467     449     346     335     251     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    170     398     989    1490    1571    1971    2063    2163    2216 
   Size:        228     591     501      81     400      92     100      53

Cuts at:   2216     170 
   Size:       3365

  Fragments arranged by size: 

                3365     591     501     400     228     100      92      81
                  53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1428    2181    3361    3542    3705    1428 
   Size:        753    1180     181     163    3134

  Fragments arranged by size: 

                3134    1180     753     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1363    1740    2654    1363 
   Size:        377     914    4120

  Fragments arranged by size: 

                4120     914     377
 
DraI TTT'AAA

Cuts at:   2410    2410 
   Size:       5411
 
DraIII CAC_nnn'GTG

Cuts at:   1848    1848 
   Size:       5411
 
DrdI GACnn_nn'nnGTC

Cuts at:   3117    3975    3117 
   Size:        858    4553
 
EaeI y'GGCC_r

Cuts at:    173     311     529     560     591     595     674     812    1190 
   Size:        138     218      31      31       4      79     138     378

Cuts at:   1190    2043    2060    2367    2996    3170    3561    3588    4933 
   Size:        853      17     307     629     174     391      27    1345

Cuts at:   4933     173 
   Size:        651

  Fragments arranged by size: 

                1345     853     651     629     391     378     307     218
                 174     138     138      79      31      31      27      17
                   4
 
EagI C'GGCC_G

Cuts at:    591    2996     591 
   Size:       2405    3006
 
EarI CTCTTCn'nnn_

Cuts at:    385     421     907    1735    3434    3644     385 
   Size:         36     486     828    1699     210    2152

  Fragments arranged by size: 

                2152    1699     828     486     210      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    211    1225    3619    3929    4075    5221     211 
   Size:       1014    2394     310     146    1146     401

  Fragments arranged by size: 

                2394    1146    1014     401     310     146
 
EcoICRI GAG'CTC

Cuts at:   1745    2423    1745 
   Size:        678    4733
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    171     232     568    1627    1671    1783    1801    1975    2326 
   Size:         61     336    1059      44     112      18     174     351

Cuts at:   2326    3236    3668    4415    4683     171 
   Size:        910     432     747     268     899

  Fragments arranged by size: 

                1059     910     899     747     432     351     336     268
                 174     112      61      44      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:    211    1516     211 
   Size:       1305    4106
 
EcoO109I rG'GnC_Cy

Cuts at:    331     332     444     459     543     957    1668    1723    1724 
   Size:          1     112      15      84     414     711      55       1

Cuts at:   1724    2550     331 
   Size:        826    3192

  Fragments arranged by size: 

                3192     826     711     414     112      84      55      15
                   1       1
 
EcoRI G'AATT_C

Cuts at:   2415    2415 
   Size:       5411
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3430    3430 
   Size:       5411
 
FseI GG_CCGG'CC

Cuts at:    597     597 
   Size:       5411
 
FspI TGC'GCA

Cuts at:    430    3192     430 
   Size:       2762    2649
 
HaeII r_GCGC'y

Cuts at:   1823    2030    2123    2845    3093    4115    5094    1823 
   Size:        207      93     722     248    1022     979    2140

  Fragments arranged by size: 

                2140    1022     979     722     248     207      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    598     630     949     981     991    1023    1891    1923    2127 
   Size:         32     319      32      10      32     868      32     204

Cuts at:   2127    2159    3253    3283    3285    3315     598 
   Size:         32    1094      30       2      30    2694

  Fragments arranged by size: 

                2694    1094     868     319     204      32      32      32
                  32      32      30      30      10       2
 
HincII GTy'rAC

Cuts at:   1755    2450    1755 
   Size:        695    4716
 
HindIII A'AGCT_T

Cuts at:    930     930 
   Size:       5411
 
KasI G'GCGC_C

Cuts at:   2026    2841    3089    2026 
   Size:        815     248    4348

  Fragments arranged by size: 

                4348     815     248
 
KpnI G_GTAC'C

Cuts at:   2431    2431 
   Size:       5411
 
MluI A'CGCG_T

Cuts at:   4844    4844 
   Size:       5411
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1160    4082    4266    1160 
   Size:       2922     184    2305

  Fragments arranged by size: 

                2922    2305     184
 
MscI TGG'CCA

Cuts at:    313     814    1192    2045    2062    2369    3172     313 
   Size:        501     378     853      17     307     803    2552

  Fragments arranged by size: 

                2552     853     803     501     378     307      17
 
MslI CAynn'nnrTG

Cuts at:    151     163     307     370     988    1054    1105    1123    1126 
   Size:         12     144      63     618      66      51      18       3

Cuts at:   1126    1129    2239    3400    3527     151 
   Size:          3    1110    1161     127    2035

  Fragments arranged by size: 

                2035    1161    1110     618     144     127      66      63
                  51      18      12       3       3
 
MspA1I CmG'CkG

Cuts at:    281    1327    1355    1433    2034    2055    2286    2325    2365 
   Size:       1046      28      78     601      21     231      39      40

Cuts at:   2365    2522    2836    3196    4209    4454     281 
   Size:        157     314     360    1013     245    1238

  Fragments arranged by size: 

                1238    1046    1013     601     360     314     245     231
                 157      78      40      39      28      21
 
NaeI GCC'GGC

Cuts at:    595    3592     595 
   Size:       2997    2414
 
NarI GG'CG_CC

Cuts at:   2027    2842    3090    2027 
   Size:        815     248    4348

  Fragments arranged by size: 

                4348     815     248
 
NcoI C'CATG_G

Cuts at:    398     989    1571    2063    2163     398 
   Size:        591     582     492     100    3646

  Fragments arranged by size: 

                3646     591     582     492     100
 
NgoMIV G'CCGG_C

Cuts at:    593    3590     593 
   Size:       2997    2414
 
NspI r_CATG'y

Cuts at:   1338    2464    2964    3871    1338 
   Size:       1126     500     907    2878

  Fragments arranged by size: 

                2878    1126     907     500
 
PasI CC'CwG_GG

Cuts at:    583    2295     583 
   Size:       1712    3699
 
PciI A'CATG_T

Cuts at:   1334    3867    1334 
   Size:       2533    2878
 
PflMI CCAn_nnn'nTGG

Cuts at:    546     546 
   Size:       5411
 
PfoI T'CCnGG_A

Cuts at:    209     440    1760    1991    2102     209 
   Size:        231    1320     231     111    3518

  Fragments arranged by size: 

                3518    1320     231     231     111
 
PmeI GTTT'AAAC

Cuts at:   2410    2410 
   Size:       5411
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1528    1560    4550    4582    1528 
   Size:         32    2990      32    2357

  Fragments arranged by size: 

                2990    2357      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    444     957    1668     444 
   Size:        513     711    4187

  Fragments arranged by size: 

                4187     711     513
 
PspOMI G'GGCC_C

Cuts at:    331     458    1723     331 
   Size:        127    1265    4019

  Fragments arranged by size: 

                4019    1265     127
 
PstI C_TGCA'G

Cuts at:    632    1295    1676    2177    2458    3143     632 
   Size:        663     381     501     281     685    2900

  Fragments arranged by size: 

                2900     685     663     501     381     281
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5411
 
PvuII CAG'CTG

Cuts at:    281    1327    2034    2286    2365    2836    3196     281 
   Size:       1046     707     252      79     471     360    2496

  Fragments arranged by size: 

                2496    1046     707     471     360     252      79
 
RsrII CG'GwC_CG

Cuts at:   3606    3606 
   Size:       5411
 
SacI G_AGCT'C

Cuts at:   1747    2425    1747 
   Size:        678    4733
 
SalI G'TCGA_C

Cuts at:   2448    2448 
   Size:       5411
 
SanDI GG'GwC_CC

Cuts at:    444     444 
   Size:       5411
 
SapI GCTCTTCn'nnn_

Cuts at:    421    1735    3434    3644     421 
   Size:       1314    1699     210    2188

  Fragments arranged by size: 

                2188    1699    1314     210
 
SbfI CC_TGCA'GG

Cuts at:   2458    2458 
   Size:       5411
 
ScaI AGT'ACT

Cuts at:   1153    1837    4928    1153 
   Size:        684    3091    1636

  Fragments arranged by size: 

                3091    1636     684
 
SexAI A'CCwGG_T

Cuts at:   1001    1001 
   Size:       5411
 
SfcI C'TryA_G

Cuts at:     33     628    1291    1503    1672    2001    2173    2381    2454 
   Size:        595     663     212     169     329     172     208      73

Cuts at:   2454    3139    4132    4323      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     663     595     329     212     208
                 191     172     169      73
 
SfoI GGC'GCC

Cuts at:   2028    2843    3091    2028 
   Size:        815     248    4348

  Fragments arranged by size: 

                4348     815     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5411
 
SmaI CCC'GGG

Cuts at:   2071    2433    2071 
   Size:        362    5049
 
SmlI C'TyrA_G

Cuts at:   2566    3481    3973    4235    2566 
   Size:        915     492     262    3742

  Fragments arranged by size: 

                3742     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5411
 
SphI G_CATG'C

Cuts at:   2464    2964    2464 
   Size:        500    4911
 
SrfI GCCC'GGGC

Cuts at:   2071    2071 
   Size:       5411
 
StyI C'CwwG_G

Cuts at:    398     989    1094    1571    2063    2163    2545    2899     398 
   Size:        591     105     477     492     100     382     354    2910

  Fragments arranged by size: 

                2910     591     492     477     382     354     105     100
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    553    1678    2290    2994    3127    3586    3795     553 
   Size:       1125     612     704     133     459     209    2169

  Fragments arranged by size: 

                2169    1125     704     612     459     209     133
 
TatI w'GTAC_w

Cuts at:   1151    1301    1337    1835    4926    1151 
   Size:        150      36     498    3091    1636

  Fragments arranged by size: 

                3091    1636     498     150      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4928    4960    4928 
   Size:         32    5379
 
Tth111I GACn'n_nGTC

Cuts at:   1003    3208    1003 
   Size:       2205    3206
 
XbaI T'CTAG_A

Cuts at:   2442    2442 
   Size:       5411
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    306     657    1125    2038    2121    2906     306 
   Size:        351     468     913      83     785    2811

  Fragments arranged by size: 

                2811     913     785     468     351      83
 
XmaI C'CCGG_G

Cuts at:   2069    2431    2069 
   Size:        362    5049
 
XmnI GAAnn'nnTTC

Cuts at:   1543    1543 
   Size:       5411
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AloI     AscI    AvrII     BaeI    BfrBI
     BmtI    BsiWI    BsmBI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI
    EcoRV    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII
     SfiI    SgrAI    SnaBI     SspI     StuI     SwaI     XhoI     ZraI
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