Restriction map for pF1KA1462
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       Aor51HI     
AscI        AseI        AsnI        Asp718I     BfrI        
BsaBI       BsiWI       BsmBI       BspDI       BspHI       
BsrDI       BssHII      Bst1107I    BstAPI      BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT22I     FseI        HpaI        KpnI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         174  GACGTC               GACGT^C
AccIII       3308  TCCGGA               T^CCGGA
AhdI         3139  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2825  GTGCAC               G^TGCAC
ApaI         2421  GGGCCC               GGGCC^C
ApaLI        2825  GTGCAC               G^TGCAC
AspEI        3139  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3556  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         793  TGCGCA               TGC^GCA
BamHI        3263  GGATCC               G^GATCC
BbeI           86  GGCGCC               GGCGC^C
BcgI         3342  CGATGCAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1950  AGATCT               A^GATCT
BseAI        3308  TCCGGA               T^CCGGA
BsiEI        1127  CGACCG               CG[GA][CT]^CG
Bsp1407I        2  TGTACA               T^GTACA
BspEI        3308  TCCGGA               T^CCGGA
BsrGI           2  TGTACA               T^GTACA
BstBI        3601  TTCGAA               TT^CGAA
DraI         1300  TTTAAA               TTT^AAA
Eam1105I     3139  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1156  CCTCCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          793  TGCGCA               TGC^GCA
HaeII          86  GGCGCC               [GA]GCGC^[CT]
HincII       1112  GTCAAC               GT[CT]^[GA]AC
HindII       1112  GTCAAC               GT[CT]^[GA]AC
HindIII      1339  AAGCTT               A^AGCTT
KasI           86  GGCGCC               G^GCGCC
MroI         3308  TCCGGA               T^CCGGA
NaeI         1386  GCCGGC               GCC^GGC
NarI           86  GGCGCC               GG^CGCC
NcoI          457  CCATGG               C^CATGG
NgoMIV       1386  GCCGGC               G^CCGGC
NspV         3601  TTCGAA               TT^CGAA
PflFI        3556  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         4014  GAAGAGC              GCTCTTC(1/4)
SfoI           86  GGCGCC               GGC^GCC
SfuI         3601  TTCGAA               TT^CGAA
SspBI           2  TGTACA               T^GTACA
TaqI         3602  TCGA                 T^CGA
Tth111I      3556  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      3602  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AflIII        192  ACATGT               A^C[GA][CT]GT
              654  ACATGT              
AocI         1210  CCTCAGG              CC^T[ACGT]AGG
             3508  CCTCAGG             
Asp700I      2930  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3701  GAAGCCCTTC          
AvrII        2986  CCTAGG               C^CTAGG
             3751  CCTAGG              
BalI         2041  TGGCCA               TGG^CCA
             2766  TGGCCA              
BbrPI         442  CACGTG               CAC^GTG
             2774  CACGTG              
BbvCI         299  CCTCAGC              CCTCAGC(-5/-2)
             2265  CCTCAGC             
BciVI          34  GGATAC               GTATCC(6/5)
              780  GTATCC              
BglI         1209  GCCTCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3496  GCCAGGCGGGC         
BlnI         2986  CCTAGG               C^CTAGG
             3751  CCTAGG              
BsaI           50  GAGACC               GGTCTC(1/5)
             1550  GAGACC              
BspLU11I      192  ACATGT               A^CATGT
              654  ACATGT              
BspMI        1651  ACCTGC               ACCTGC(4/8)
             2905  GCAGGT              
BsrBI         920  GAGCGG               CCGCTC(-3/-3)
             3314  GAGCGG              
BstYI        1950  AGATCT               [GA]^GATC[CT]
             3263  GGATCC              
Bsu36I       1210  CCTCAGG              CC^T[ACGT]AGG
             3508  CCTCAGG             
CfoI           87  GCGC                 GCG^C
              794  GCGC                
Eco81I       1210  CCTCAGG              CC^T[ACGT]AGG
             3508  CCTCAGG             
EcoRI         663  GAATTC               G^AATTC
              819  GAATTC              
HhaI           87  GCGC                 GCG^C
              794  GCGC                
HinP1I         87  GCGC                 G^CGC
              794  GCGC                
KspI          179  CCGCGG               CCGC^GG
             2643  CCGCGG              
MflI         1950  AGATCT               [GA]^GATC[CT]
             3263  GGATCC              
MluNI        2041  TGGCCA               TGG^CCA
             2766  TGGCCA              
MscI         2041  TGGCCA               TGG^CCA
             2766  TGGCCA              
PflMI         948  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3933  CCAGAGATTGG         
PmaCI         442  CACGTG               CAC^GTG
             2774  CACGTG              
PmlI          442  CACGTG               CAC^GTG
             2774  CACGTG              
PpuMI         528  AGGTCCT              [GA]G^G[AT]CC[CT]
             2260  AGGTCCC             
PvuII        2107  CAGCTG               CAG^CTG
             2978  CAGCTG              
SacII         179  CCGCGG               CCGC^GG
             2643  CCGCGG              
StuI         1464  AGGCCT               AGG^CCT
             3900  AGGCCT              
Van91I        948  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3933  CCAGAGATTGG         
XhoII        1950  AGATCT               [GA]^GATC[CT]
             3263  GGATCC              
XmnI         2930  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3701  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AgeI        AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CelII       Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        Hin1I       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PinAI       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmaCI       XmaI        
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