Restriction map for pF1KA1449
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       FauI        FbaI        FseI        
FspI        HgaI        Hin1I       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SphI        Sse8387I    SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AseI          267  ATTAAT               AT^TAAT
AsnI          267  ATTAAT               AT^TAAT
Asp700I      1971  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1608  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI         1598  CCGGG                CC^[GC]GG
BglI          674  GCCTGTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1188  AGATCT               A^GATCT
BsaAI         355  TACGTA               [CT]AC^GT[GA]
BsaI         1997  GAGACC               GGTCTC(1/5)
BsiEI         144  CGGTCG               CG[GA][CT]^CG
BsmI          306  GAATGC               GAATGC(1/-1)
BspMI          45  GCAGGT               ACCTGC(4/8)
BsrBI        1989  GAGCGG               CCGCTC(-3/-3)
BstBI        1961  TTCGAA               TT^CGAA
BstXI        1470  CCATGTAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          769  GCGC                 GCG^C
DraI         1231  TTTAAA               TTT^AAA
EcoRI         983  GAATTC               G^AATTC
EcoT22I      1715  ATGCAT               ATGCA^T
HaeII         768  GGCGCT               [GA]GCGC^[CT]
HhaI          769  GCGC                 GCG^C
HinP1I        769  GCGC                 G^CGC
HindIII       613  AAGCTT               A^AGCTT
HpaI         1284  GTTAAC               GTT^AAC
MaeII         356  ACGT                 A^CGT
NciI         1598  CCGGG                CC^[GC]GG
NsiI         1715  ATGCAT               ATGCA^T
NspV         1961  TTCGAA               TT^CGAA
PflFI        1608  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1935  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         267  ATTAAT               AT^TAAT
PstI          106  CTGCAG               CTGCA^G
SfuI         1961  TTCGAA               TT^CGAA
SmlI          892  CTCAAG               C^T[CT][GA]AG
SnaBI         355  TACGTA               TAC^GTA
SpeI          390  ACTAGT               A^CTAGT
StuI          362  AGGCCT               AGG^CCT
Tth111I      1608  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1935  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1971  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          954  GTCTAC               GT^[AC][GT]AC
             1264  GTAGAC              
BalI          521  TGGCCA               TGG^CCA
              717  TGGCCA              
BmyI          622  GGGCAC               G[AGT]GC[ACT]^C
             1525  GTGCCC              
BpmI         1004  CTGGAG               CTGGAG(16/14)
             1559  CTGGAG              
BseRI         923  CTCCTC               GAGGAG(10/8)
             1906  GAGGAG              
BsgI           43  GTGCAG               GTGCAG(16/14)
             1498  GTGCAG              
Bsp1286I      622  GGGCAC               G[AGT]GC[ACT]^C
             1525  GTGCCC              
BsrDI         644  CATTGC               GCAATG(2/0)
              669  GCAATG              
BsrFI          14  ACCGGC               [GA]^CCGG[CT]
              142  GCCGGT              
BsrI          534  ACTGG                ACTGG(1/-1)
              886  CCAGT               
Cac8I         665  GCACGC               GC[ACGT]^[ACGT]GC
              719  GCCAGC              
Cfr10I         14  ACCGGC               [GA]^CCGG[CT]
              142  GCCGGT              
EaeI          521  TGGCCA               [CT]^GGCC[GA]
              717  TGGCCA              
EcoT14I       377  CCAAGG               C^C[AT][AT]GG
              714  CCTTGG              
HincII        330  GTCAAC               GT[CT]^[GA]AC
             1284  GTTAAC              
HindII        330  GTCAAC               GT[CT]^[GA]AC
             1284  GTTAAC              
MluNI         521  TGGCCA               TGG^CCA
              717  TGGCCA              
MscI          521  TGGCCA               TGG^CCA
              717  TGGCCA              
NdeI          371  CATATG               CA^TATG
             1980  CATATG              
SapI         1986  GAAGAGC              GCTCTTC(1/4)
             1844  GCTCTTC             
SexAI        1061  ACCAGGT              A^CC[AT]GGT
             1934  ACCAGGT             
SfcI          106  CTGCAG               C^T[GA][CT]AG
              956  CTACAG              
SspI          420  AATATT               AAT^ATT
             1415  AATATT              
StyI          377  CCAAGG               C^C[AT][AT]GG
              714  CCTTGG              
Tsp45I        475  GTCAC                ^GT[GC]AC
             1763  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AvaII       BamHI       BbvI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1407I    BspLU11I    BsrGI       BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SspBI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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