Restriction map for pF1KA1352
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AspEI       AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       CelII       CfoI        ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        Sse8387I    StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        224  GGTACC               G^GTACC
AccII        2011  CGCG                 CG^CG
AflIII        188  ACACGT               A^C[GA][CT]GT
AseI         1053  ATTAAT               AT^TAAT
AsnI         1053  ATTAAT               AT^TAAT
Asp700I      1313  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       224  GGTACC               G^GTACC
AspHI        2602  GAGCAC               G[AT]GC[AT]^C
AspI         1261  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3234  TGGCCA               TGG^CCA
BcnI         1368  CCGGG                CC^[GC]GG
BmyI         2602  GAGCAC               G[AGT]GC[ACT]^C
BsaAI        2864  TACGTA               [CT]AC^GT[GA]
BsaBI         625  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2546  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      2602  GAGCAC               G[AT]GC[AT]^C
Bsp1286I     2602  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I     2300  TGTACA               T^GTACA
BsrFI        1281  GCCGGT               [GA]^CCGG[CT]
BsrGI        2300  TGTACA               T^GTACA
Bst1107I      708  GTATAC               GTA^TAC
BstUI        2011  CGCG                 CG^CG
BstZ17I       708  GTATAC               GTA^TAC
Cfr10I       1281  GCCGGT               [GA]^CCGG[CT]
DrdI         2902  GACAACAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2273  CCATGG               C^C[GA][CT]GG
EcoRI        1987  GAATTC               G^AATTC
EcoT22I        98  ATGCAT               ATGCA^T
FauI          552  CCCGC                CCCGC(4/6)
HindIII      2456  AAGCTT               A^AGCTT
KpnI          224  GGTACC               GGTAC^C
MamI          625  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        3234  TGGCCA               TGG^CCA
MscI         3234  TGGCCA               TGG^CCA
MvnI         2011  CGCG                 CG^CG
NciI         1368  CCGGG                CC^[GC]GG
NcoI         2273  CCATGG               C^CATGG
NsiI           98  ATGCAT               ATGCA^T
PflFI        1261  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2664  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1053  ATTAAT               AT^TAAT
PstI         2788  CTGCAG               CTGCA^G
PvuII        2105  CAGCTG               CAG^CTG
SexAI        3192  ACCTGGT              A^CC[AT]GGT
SnaBI        2864  TACGTA               TAC^GTA
SphI         3429  GCATGC               GCATG^C
SspBI        2300  TGTACA               T^GTACA
SspI          856  AATATT               AAT^ATT
Tth111I      1261  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1313  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          595  GTAGAC               GT^[AC][GT]AC
              708  GTATAC              
AcyI          170  GACGCC               G[GA]^CG[CT]C
              602  GGCGTC              
AlwNI        1762  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2663  CAGGTCCTG           
AocI          781  CCTCAGG              CC^T[ACGT]AGG
             1691  CCTTAGG             
BciVI          72  GGATAC               GTATCC(6/5)
             1995  GTATCC              
BsaHI         170  GACGCC               G[GA]^CG[CT]C
              602  GGCGTC              
BsaI          889  GAGACC               GGTCTC(1/5)
             1666  GAGACC              
BsgI          268  CTGCAC               GTGCAG(16/14)
             1088  CTGCAC              
BsmBI        2013  CGTCTC               CGTCTC(1/5)
              949  GAGACG              
BsmFI         183  GGGAC                GGGAC(10/14)
             2342  GGGAC               
BspHI         750  TCATGA               T^CATGA
             3452  TCATGA              
BsrDI        2803  CATTGC               GCAATG(2/0)
             2913  CATTGC              
BstXI        2258  CCAACTTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2810  CCATCTATGTGG        
BstYI         454  GGATCT               [GA]^GATC[CT]
             2025  GGATCT              
Bsu36I        781  CCTCAGG              CC^T[ACGT]AGG
             1691  CCTTAGG             
EaeI         2077  TGGCCG               [CT]^GGCC[GA]
             3234  TGGCCA              
Eco81I        781  CCTCAGG              CC^T[ACGT]AGG
             1691  CCTTAGG             
Hin1I         170  GACGCC               G[GA]^CG[CT]C
              602  GGCGTC              
MaeII         190  ACGT                 A^CGT
             2865  ACGT                
MflI          454  GGATCT               [GA]^GATC[CT]
             2025  GGATCT              
MspA1I       1864  CCGCTG               C[AC]G^C[GT]G
             2105  CAGCTG              
PflMI        1303  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3226  CCATCCAATGG         
PshAI         598  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3151  GACTGCTGTC          
RcaI          750  TCATGA               T^CATGA
             3452  TCATGA              
TaqI         3312  TCGA                 T^CGA
             3375  TCGA                
TthHB8I      3312  TCGA                 T^CGA
             3375  TCGA                
Van91I       1303  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3226  CCATCCAATGG         
XcmI         1790  CCACTATTTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3222  CCAGCCATCCAATGG     
XhoII         454  GGATCT               [GA]^GATC[CT]
             2025  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BbsI        BbvI        
BfaI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmI        BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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