Restriction map for pF1KA1352
 (Circular) (Six-base) MAPSORT of: pF1KA1352.seq  Check: 7070  from: 1  to: 6641



pF1KA1352  6641 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1862    1862 
   Size:       6641
 
AccI GT'mk_AC

Cuts at:    704     817    3679     704 
   Size:        113    2862    3666

  Fragments arranged by size: 

                3666    2862     113
 
Acc65I G'GTAC_C

Cuts at:    332    3657     332 
   Size:       3325    3316
 
AclI AA'CG_TT

Cuts at:   6481    6481 
   Size:       6641
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    653    3073    4466    4898    5645    5913     653 
   Size:       2420    1393     432     747     268    1381

  Fragments arranged by size: 

                2420    1393    1381     747     432     268
 
AflIII A'CryG_T

Cuts at:    296    3993    5097    6074     296 
   Size:       3697    1104     977     863

  Fragments arranged by size: 

                3697    1104     977     863
 
AgeI A'CCGG_T

Cuts at:   5029    5029 
   Size:       6641
 
AleI CACnn'nnGTG

Cuts at:   1267    2703    1267 
   Size:       1436    5205
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1870    1870 
   Size:       6641
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1550    1582    2636    2668    1550 
   Size:         32    1054      32    5523

  Fragments arranged by size: 

                5523    1054      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1875    2776    3946    5513    1875 
   Size:        901    1170    1567    3003

  Fragments arranged by size: 

                3003    1567    1170     901
 
ApaLI G'TGCA_C

Cuts at:   5411    5411 
   Size:       6641
 
ApoI r'AATT_y

Cuts at:    625     802    1175    1457    1785    2095    2305    3145    3572 
   Size:        177     373     282     328     310     210     840     427

Cuts at:   3572    3645    5994    6250     625 
   Size:         73    2349     256    1016

  Fragments arranged by size: 

                2349    1016     840     427     373     328     310     282
                 256     210     177      73
 
AseI AT'TA_AT

Cuts at:     20    1162      20 
   Size:       1142    5499
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6641
 
AvaI C'yCGr_G

Cuts at:   3661    3661 
   Size:       6641
 
BamHI G'GATC_C

Cuts at:   3666    3666 
   Size:       6641
 
BanI G'GyrC_C

Cuts at:    332    1143    1392    3657    4071    4319    4354     332 
   Size:        811     249    2265     414     248      35    2619

  Fragments arranged by size: 

                2619    2265     811     414     249     248      35
 
BanII G_rGCy'C

Cuts at:   3655    4685    3655 
   Size:       1030    5611
 
BbeI G_GCGC'C

Cuts at:   4075    4323    4075 
   Size:        248    6393
 
BbsI GAAGACnn'nnnn_

Cuts at:   1588    2884    3263    3526    1588 
   Size:       1296     379     263    4703

  Fragments arranged by size: 

                4703    1296     379     263
 
BciVI GTATCCnnnnn_n'

Cuts at:    174    2114    4539    5300    6641     174 
   Size:       1940    2425     761    1341     174

  Fragments arranged by size: 

                2425    1940    1341     761     174
 
BclI T'GATC_A

Cuts at:   4161    4161 
   Size:       6641
 
BfrBI ATG'CAT

Cuts at:    208     208 
   Size:       6641
 
BglII A'GATC_T

Cuts at:   4156    6193    4156 
   Size:       2037    4604
 
BlpI GC'TnA_GC

Cuts at:   3753    3753 
   Size:       6641
 
Bme1580I G_kGCm'C

Cuts at:   4266    4359    5415    4266 
   Size:         93    1056    5492

  Fragments arranged by size: 

                5492    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2457    3853    3934    4269    6413    2457 
   Size:       1396      81     335    2144    2685

  Fragments arranged by size: 

                2685    2144    1396     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5008    5008 
   Size:       6641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    893    1631    3127     893 
   Size:        738    1496    4407

  Fragments arranged by size: 

                4407    1496     738
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    943    1812    2830    3220    3817    4696    5188    5486     943 
   Size:        869    1018     390     597     879     492     298    2098

  Fragments arranged by size: 

                2098    1018     879     869     597     492     390     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    991    1768    6345     991 
   Size:        777    4577    1287

  Fragments arranged by size: 

                4577    1287     777
 
BsaAI yAC'GTr

Cuts at:   2974    3994    2974 
   Size:       1020    5621
 
BsaBI GATnn'nnATC

Cuts at:      6     737    4155    6192       6 
   Size:        731    3418    2037     455

  Fragments arranged by size: 

                3418    2037     731     455
 
BsaHI Gr'CG_yC

Cuts at:    279     711    4072    4320    5022    6518     279 
   Size:        432    3361     248     702    1496     402

  Fragments arranged by size: 

                3361    1496     702     432     402     248
 
BsaWI w'CCGG_w

Cuts at:   2654    3830    4351    5029    5303    5450    5861    2654 
   Size:       1176     521     678     274     147     411    3434

  Fragments arranged by size: 

                3434    1176     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    866     896    2222    2252     866 
   Size:         30    1326      30    5255

  Fragments arranged by size: 

                5255    1326      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1786    2227    2671    2790    1786 
   Size:        441     444     119    5637

  Fragments arranged by size: 

                5637     444     441     119
 
BseYI C'CCAG_C

Cuts at:   1974    3329    4066    4824    5401    5897    1974 
   Size:       1355     737     758     577     496    2718

  Fragments arranged by size: 

                2718    1355     758     737     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    361    1181     361 
   Size:        820    5821
 
BsiEI CG_ry'CG

Cuts at:    104    4183    4229    5437     104 
   Size:       4079      46    1208    1308

  Fragments arranged by size: 

                4079    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2714    3655    4433    4623    5415    2714 
   Size:        941     778     190     792    3940

  Fragments arranged by size: 

                3940     941     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    395    2327    2517    2757     395 
   Size:       1932     190     240    4279

  Fragments arranged by size: 

                4279    1932     240     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1051    2127    1051 
   Size:       1076    5565
 
Bsp1286I G_dGCh'C

Cuts at:   2714    3655    4266    4359    4433    4623    4685    5415    2714 
   Size:        941     611      93      74     190      62     730    3940

  Fragments arranged by size: 

                3940     941     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   5861    5861 
   Size:       6641
 
BspHI T'CATG_A

Cuts at:    858    3560    5817     858 
   Size:       2702    2257    1682

  Fragments arranged by size: 

                2702    2257    1682
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1862    2383    2761    3691    3905    4207    4588    1862 
   Size:        521     378     930     214     302     381    3915

  Fragments arranged by size: 

                3915     930     521     381     378     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4933    4987    6471    6636      50 
   Size:       4883      54    1484     165      55

  Fragments arranged by size: 

                4883    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2910    3020    4553    2910 
   Size:        110    1533    4998

  Fragments arranged by size: 

                4998    1533     110
 
BsrFI r'CCGG_y

Cuts at:   1389    4639    4820    5029    1389 
   Size:       3250     181     209    3001

  Fragments arranged by size: 

                3250    3001     209     181
 
BsrGI T'GTAC_A

Cuts at:   2408    2408 
   Size:       6641
 
BssSI C'ACGA_G

Cuts at:   4912    5270    4912 
   Size:        358    6283
 
BstAPI GCAn_nnn'nTGC

Cuts at:   4018    4018 
   Size:       6641
 
BstBI TT'CG_AA

Cuts at:   5002    5002 
   Size:       6641
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2373    2925    2373 
   Size:        552    6089
 
BstYI r'GATC_y

Cuts at:      1     562    2133    3666    4133    4156    4491    4737    5749 
   Size:        561    1571    1533     467      23     335     246    1012

Cuts at:   5749    5835    5847    6193       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1571    1533    1012     561     467     449     346     335
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    818     818 
   Size:       6641
 
Bsu36I CC'TnA_GG

Cuts at:    890    1800     890 
   Size:        910    5731
 
BtgI C'CryG_G

Cuts at:    107    2381     107 
   Size:       2274    4367
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4591    4772    4935    4591 
   Size:        181     163    6297

  Fragments arranged by size: 

                6297     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1876    3884    1876 
   Size:       2008    4633
 
DraI TTT'AAA

Cuts at:    683    1554    2559    3640     683 
   Size:        871    1005    1081    3684

  Fragments arranged by size: 

                3684    1081    1005     871
 
DrdI GACnn_nn'nnGTC

Cuts at:   3016    4347    5205    3016 
   Size:       1331     858    4452

  Fragments arranged by size: 

                4452    1331     858
 
EaeI y'GGCC_r

Cuts at:   2185    3342    4226    4400    4791    4818    6163    2185 
   Size:       1157     884     174     391      27    1345    2663

  Fragments arranged by size: 

                2663    1345    1157     884     391     174      27
 
EagI C'GGCC_G

Cuts at:   4226    4226 
   Size:       6641
 
EarI CTCTTCn'nnn_

Cuts at:    464     470     608    1709    4664    4874     464 
   Size:          6     138    1101    2955     210    2231

  Fragments arranged by size: 

                2955    2231    1101     210     138       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    4849    5159    5305    6451     127 
   Size:       4722     310     146    1146     317

  Fragments arranged by size: 

                4722    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:   3653    3653 
   Size:       6641
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    653     893    1631    3073    3127    4466    4898    5645    5913 
   Size:        240     738    1442      54    1339     432     747     268

Cuts at:   5913     653 
   Size:       1381

  Fragments arranged by size: 

                1442    1381    1339     747     738     432     268     240
                  54
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1936    2768    3502    1936 
   Size:        832     734    5075

  Fragments arranged by size: 

                5075     832     734
 
EcoO109I rG'GnC_Cy

Cuts at:   2773    3118    3478    3780    2773 
   Size:        345     360     302    5634

  Fragments arranged by size: 

                5634     360     345     302
 
EcoRI G'AATT_C

Cuts at:   2095    3645    2095 
   Size:       1550    5091
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1301    1356    4660    1301 
   Size:         55    3304    3282

  Fragments arranged by size: 

                3304    3282      55
 
FspI TGC'GCA

Cuts at:   4422    4422 
   Size:       6641
 
HaeII r_GCGC'y

Cuts at:   4075    4323    5345    6324    4075 
   Size:        248    1022     979    4392

  Fragments arranged by size: 

                4392    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2222    2242    2254    2274    2828    2860    3105    3137    3479 
   Size:         20      12      20     554      32     245      32     342

Cuts at:   3479    3511    4483    4513    4515    4545    2222 
   Size:         32     972      30       2      30    4318

  Fragments arranged by size: 

                4318     972     554     342     245      32      32      32
                  30      30      20      20      12       2
 
HincII GTy'rAC

Cuts at:   3680    3680 
   Size:       6641
 
HindIII A'AGCT_T

Cuts at:   2564    2564 
   Size:       6641
 
KasI G'GCGC_C

Cuts at:   4071    4319    4071 
   Size:        248    6393
 
KpnI G_GTAC'C

Cuts at:    336    3661     336 
   Size:       3325    3316
 
MluI A'CGCG_T

Cuts at:   6074    6074 
   Size:       6641
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    192     864    1313    5312    5496     192 
   Size:        672     449    3999     184    1337

  Fragments arranged by size: 

                3999    1337     672     449     184
 
MscI TGG'CCA

Cuts at:   3344    4402    3344 
   Size:       1058    5583
 
MslI CAynn'nnrTG

Cuts at:   1267    1540    1740    2703    2705    2923    4630    4757    1267 
   Size:        273     200     963       2     218    1707     127    3151

  Fragments arranged by size: 

                3151    1707     963     273     218     200     127       2
            
 
MspA1I CmG'CkG

Cuts at:   1974    2215    3752    4066    4426    5439    5684    1974 
   Size:        241    1537     314     360    1013     245    2931

  Fragments arranged by size: 

                2931    1537    1013     360     314     245     241
 
NaeI GCC'GGC

Cuts at:   4822    4822 
   Size:       6641
 
NarI GG'CG_CC

Cuts at:   4072    4320    4072 
   Size:        248    6393
 
NcoI C'CATG_G

Cuts at:    107    2381     107 
   Size:       2274    4367
 
NgoMIV G'CCGG_C

Cuts at:   4820    4820 
   Size:       6641
 
NsiI A_TGCA'T

Cuts at:    210     210 
   Size:       6641
 
NspI r_CATG'y

Cuts at:    407    1822    3541    3694    4194    5101     407 
   Size:       1415    1719     153     500     907    1947

  Fragments arranged by size: 

                1947    1719    1415     907     500     153
 
PciI A'CATG_T

Cuts at:   5097    5097 
   Size:       6641
 
PflMI CCAn_nnn'nTGG

Cuts at:   1417    3340    1417 
   Size:       1923    4718
 
PmeI GTTT'AAAC

Cuts at:   3640    3640 
   Size:       6641
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5780    5812    5780 
   Size:         32    6609
 
PpuMI rG'GwC_Cy

Cuts at:   2773    2773 
   Size:       6641
 
PshAI GACnn'nnGTC

Cuts at:    710    3263     710 
   Size:       2553    4088
 
PsiI TTA'TAA

Cuts at:   2168    2524    2168 
   Size:        356    6285
 
PstI C_TGCA'G

Cuts at:   2900    3688    4373    2900 
   Size:        788     685    5168

  Fragments arranged by size: 

                5168     788     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6641
 
PvuII CAG'CTG

Cuts at:   2215    4066    4426    2215 
   Size:       1851     360    4430

  Fragments arranged by size: 

                4430    1851     360
 
RsrII CG'GwC_CG

Cuts at:   4836    4836 
   Size:       6641
 
SacI G_AGCT'C

Cuts at:   3655    3655 
   Size:       6641
 
SalI G'TCGA_C

Cuts at:   3678    3678 
   Size:       6641
 
SapI GCTCTTCn'nnn_

Cuts at:   4664    4874    4664 
   Size:        210    6431
 
SbfI CC_TGCA'GG

Cuts at:   3688    3688 
   Size:       6641
 
ScaI AGT'ACT

Cuts at:   6158    6158 
   Size:       6641
 
SexAI A'CCwGG_T

Cuts at:   3300    3300 
   Size:       6641
 
SfcI C'TryA_G

Cuts at:     33     326    2210    2354    2896    3684    4369    5362    5553 
   Size:        293    1884     144     542     788     685     993     191

Cuts at:   5553      33 
   Size:       1121

  Fragments arranged by size: 

                1884    1121     993     788     685     542     293     191
                 144
 
SfoI GGC'GCC

Cuts at:   4073    4321    4073 
   Size:        248    6393
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6641
 
SmaI CCC'GGG

Cuts at:   3663    3663 
   Size:       6641
 
SmlI C'TyrA_G

Cuts at:    922    1827    2845    3199    3796    4711    5203    5465     922 
   Size:        905    1018     354     597     915     492     262    2098

  Fragments arranged by size: 

                2098    1018     915     905     597     492     354     262
            
 
SnaBI TAC'GTA

Cuts at:   2974    2974 
   Size:       6641
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6641
 
SphI G_CATG'C

Cuts at:   3541    3694    4194    3541 
   Size:        153     500    5988

  Fragments arranged by size: 

                5988     500     153
 
SspI AAT'ATT

Cuts at:    966     966 
   Size:       6641
 
StyI C'CwwG_G

Cuts at:    107     597    1858    2000    2381    2944    3775    4129     107 
   Size:        490    1261     142     381     563     831     354    2619

  Fragments arranged by size: 

                2619    1261     831     563     490     381     354     142
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2415    4224    4357    4816    5025    2415 
   Size:       1809     133     459     209    4031

  Fragments arranged by size: 

                4031    1809     459     209     133
 
TatI w'GTAC_w

Cuts at:    382    1043    2408    3509    6156     382 
   Size:        661    1365    1101    2647     867

  Fragments arranged by size: 

                2647    1365    1101     867     661
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1855    1887    6158    6190    1855 
   Size:         32    4271      32    2306

  Fragments arranged by size: 

                4271    2306      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1372    4438    1372 
   Size:       3066    3575
 
XbaI T'CTAG_A

Cuts at:   3672    3672 
   Size:       6641
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1905    3337    4136    1905 
   Size:       1432     799    4410

  Fragments arranged by size: 

                4410    1432     799
 
XmaI C'CCGG_G

Cuts at:   3661    3661 
   Size:       6641
 
XmnI GAAnn'nnTTC

Cuts at:   1425    1425 
   Size:       6641
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NsiI     NspI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     ApaI     AscI    AvrII     BaeI
    BbvCI     BcgI     BglI    BmgBI     BmtI   Bpu10I    BsiWI   BssHII
   BstEII     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NruI     PacI     PasI     PfoI
     PmlI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
     SrfI     StuI     SwaI     XhoI     ZraI
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