Restriction map for pF1KA1352
 (Linear) (Six-base) MAPSORT of: KIAA1352.seq  Check: 6037  from: 1  to: 3528



KIAA1352.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1754    3528 
   Size:       1754    1774
 
AccI GT'mk_AC

Cuts at:      0     596     709    3528 
   Size:        596     113    2819

  Fragments arranged by size: 

                2819     596     113
 
Acc65I G'GTAC_C

Cuts at:      0     224    3528 
   Size:        224    3304
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    2965    3528 
   Size:        545    2420     563

  Fragments arranged by size: 

                2420     563     545
 
AflIII A'CryG_T

Cuts at:      0     188    3528 
   Size:        188    3340
 
AleI CACnn'nnGTG

Cuts at:      0    1159    2595    3528 
   Size:       1159    1436     933

  Fragments arranged by size: 

                1436    1159     933
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1762    3528 
   Size:       1762    1766
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1442    1474    2528    2560    3528 
   Size:       1442      32    1054      32     968

  Fragments arranged by size: 

                1442    1054     968      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1767    2668    3528 
   Size:       1767     901     860

  Fragments arranged by size: 

                1767     901     860
 
ApoI r'AATT_y

Cuts at:      0     517     694    1067    1349    1677    1987    2197    3037 
   Size:        517     177     373     282     328     310     210     840

Cuts at:   3037    3464    3528 
   Size:        427      64

  Fragments arranged by size: 

                 840     517     427     373     328     310     282     210
                 177      64
 
AseI AT'TA_AT

Cuts at:      0    1054    3528 
   Size:       1054    2474
 
BanI G'GyrC_C

Cuts at:      0     224    1035    1284    3528 
   Size:        224     811     249    2244

  Fragments arranged by size: 

                2244     811     249     224
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1480    2776    3155    3418    3528 
   Size:       1480    1296     379     263     110

  Fragments arranged by size: 

                1480    1296     379     263     110
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      66    2006    3528 
   Size:         66    1940    1522

  Fragments arranged by size: 

                1940    1522      66
 
BfrBI ATG'CAT

Cuts at:      0     100    3528 
   Size:        100    3428
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2349    3528 
   Size:       2349    1179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1523    3019    3528 
   Size:        785     738    1496     509

  Fragments arranged by size: 

                1496     785     738     509
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1704    2722    3112    3528 
   Size:        835     869    1018     390     416

  Fragments arranged by size: 

                1018     869     835     416     390
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     883    1660    3528 
   Size:        883     777    1868

  Fragments arranged by size: 

                1868     883     777
 
BsaAI yAC'GTr

Cuts at:      0    2866    3528 
   Size:       2866     662
 
BsaBI GATnn'nnATC

Cuts at:      0     629    3528 
   Size:        629    2899
 
BsaHI Gr'CG_yC

Cuts at:      0     171     603    3528 
   Size:        171     432    2925

  Fragments arranged by size: 

                2925     432     171
 
BsaWI w'CCGG_w

Cuts at:      0    2546    3528 
   Size:       2546     982
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     758     788    2114    2144    3528 
   Size:        758      30    1326      30    1384

  Fragments arranged by size: 

                1384    1326     758      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1678    2119    2563    2682    3528 
   Size:       1678     441     444     119     846

  Fragments arranged by size: 

                1678     846     444     441     119
 
BseYI C'CCAG_C

Cuts at:      0    1866    3221    3528 
   Size:       1866    1355     307

  Fragments arranged by size: 

                1866    1355     307
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253    1073    3528 
   Size:        253     820    2455

  Fragments arranged by size: 

                2455     820     253
 
BsiHKAI G_wGCw'C

Cuts at:      0    2606    3528 
   Size:       2606     922
 
BsmI GAATG_Cn'

Cuts at:      0     287    2219    2409    2649    3528 
   Size:        287    1932     190     240     879

  Fragments arranged by size: 

                1932     879     287     240     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     943    2019    3528 
   Size:        943    1076    1509

  Fragments arranged by size: 

                1509    1076     943
 
Bsp1286I G_dGCh'C

Cuts at:      0    2606    3528 
   Size:       2606     922
 
BspHI T'CATG_A

Cuts at:      0     750    3452    3528 
   Size:        750    2702      76

  Fragments arranged by size: 

                2702     750      76
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1754    2275    2653    3528 
   Size:       1754     521     378     875

  Fragments arranged by size: 

                1754     875     521     378
 
BsrDI GCAATG_nn'

Cuts at:      0    2802    2912    3528 
   Size:       2802     110     616

  Fragments arranged by size: 

                2802     616     110
 
BsrFI r'CCGG_y

Cuts at:      0    1281    3528 
   Size:       1281    2247
 
BsrGI T'GTAC_A

Cuts at:      0    2300    3528 
   Size:       2300    1228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2265    2817    3528 
   Size:       2265     552     711

  Fragments arranged by size: 

                2265     711     552
 
BstYI r'GATC_y

Cuts at:      0     454    2025    3528 
   Size:        454    1571    1503

  Fragments arranged by size: 

                1571    1503     454
 
BstZ17I GTA'TAC

Cuts at:      0     710    3528 
   Size:        710    2818
 
Bsu36I CC'TnA_GG

Cuts at:      0     782    1692    3528 
   Size:        782     910    1836

  Fragments arranged by size: 

                1836     910     782
 
BtgI C'CryG_G

Cuts at:      0    2273    3528 
   Size:       2273    1255
 
BtsI GCAGTG_nn'

Cuts at:      0    1768    3528 
   Size:       1768    1760
 
DraI TTT'AAA

Cuts at:      0     575    1446    2451    3528 
   Size:        575     871    1005    1077

  Fragments arranged by size: 

                1077    1005     871     575
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2908    3528 
   Size:       2908     620
 
EaeI y'GGCC_r

Cuts at:      0    2077    3234    3528 
   Size:       2077    1157     294

  Fragments arranged by size: 

                2077    1157     294
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     362     500    1601    3528 
   Size:        356       6     138    1101    1927

  Fragments arranged by size: 

                1927    1101     356     138       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    3528 
   Size:         19    3509
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     785    1523    2965    3019    3528 
   Size:        545     240     738    1442      54     509

  Fragments arranged by size: 

                1442     738     545     509     240      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1828    2660    3394    3528 
   Size:       1828     832     734     134

  Fragments arranged by size: 

                1828     832     734     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2665    3010    3370    3528 
   Size:       2665     345     360     158

  Fragments arranged by size: 

                2665     360     345     158
 
EcoRI G'AATT_C

Cuts at:      0    1987    3528 
   Size:       1987    1541
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1193    1248    3528 
   Size:       1193      55    2280

  Fragments arranged by size: 

                2280    1193      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2114    2134    2146    2166    2720    2752    2997    3029 
   Size:       2114      20      12      20     554      32     245      32

Cuts at:   3029    3371    3403    3528 
   Size:        342      32     125

  Fragments arranged by size: 

                2114     554     342     245     125      32      32      32
                  20      20      12
 
HindIII A'AGCT_T

Cuts at:      0    2456    3528 
   Size:       2456    1072
 
KpnI G_GTAC'C

Cuts at:      0     228    3528 
   Size:        228    3300
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     756    1205    3528 
   Size:         84     672     449    2323

  Fragments arranged by size: 

                2323     672     449      84
 
MscI TGG'CCA

Cuts at:      0    3236    3528 
   Size:       3236     292
 
MslI CAynn'nnrTG

Cuts at:      0    1159    1432    1632    2595    2597    2815    3528 
   Size:       1159     273     200     963       2     218     713

  Fragments arranged by size: 

                1159     963     713     273     218     200       2
 
MspA1I CmG'CkG

Cuts at:      0    1866    2107    3528 
   Size:       1866     241    1421

  Fragments arranged by size: 

                1866    1421     241
 
NcoI C'CATG_G

Cuts at:      0    2273    3528 
   Size:       2273    1255
 
NsiI A_TGCA'T

Cuts at:      0     102    3528 
   Size:        102    3426
 
NspI r_CATG'y

Cuts at:      0     299    1714    3433    3528 
   Size:        299    1415    1719      95

  Fragments arranged by size: 

                1719    1415     299      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1309    3232    3528 
   Size:       1309    1923     296

  Fragments arranged by size: 

                1923    1309     296
 
PpuMI rG'GwC_Cy

Cuts at:      0    2665    3528 
   Size:       2665     863
 
PshAI GACnn'nnGTC

Cuts at:      0     602    3155    3528 
   Size:        602    2553     373

  Fragments arranged by size: 

                2553     602     373
 
PsiI TTA'TAA

Cuts at:      0    2060    2416    3528 
   Size:       2060     356    1112

  Fragments arranged by size: 

                2060    1112     356
 
PstI C_TGCA'G

Cuts at:      0    2792    3528 
   Size:       2792     736
 
PvuII CAG'CTG

Cuts at:      0    2107    3528 
   Size:       2107    1421
 
SexAI A'CCwGG_T

Cuts at:      0    3192    3528 
   Size:       3192     336
 
SfcI C'TryA_G

Cuts at:      0     218    2102    2246    2788    3528 
   Size:        218    1884     144     542     740

  Fragments arranged by size: 

                1884     740     542     218     144
 
SmlI C'TyrA_G

Cuts at:      0     814    1719    2737    3091    3528 
   Size:        814     905    1018     354     437

  Fragments arranged by size: 

                1018     905     814     437     354
 
SnaBI TAC'GTA

Cuts at:      0    2866    3528 
   Size:       2866     662
 
SphI G_CATG'C

Cuts at:      0    3433    3528 
   Size:       3433      95
 
SspI AAT'ATT

Cuts at:      0     858    3528 
   Size:        858    2670
 
StyI C'CwwG_G

Cuts at:      0     489    1750    1892    2273    2836    3528 
   Size:        489    1261     142     381     563     692

  Fragments arranged by size: 

                1261     692     563     489     381     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2307    3528 
   Size:       2307    1221
 
TatI w'GTAC_w

Cuts at:      0     274     935    2300    3401    3528 
   Size:        274     661    1365    1101     127

  Fragments arranged by size: 

                1365    1101     661     274     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1747    1779    3528 
   Size:       1747      32    1749

  Fragments arranged by size: 

                1749    1747      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1264    3528 
   Size:       1264    2264
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1797    3229    3528 
   Size:       1797    1432     299

  Fragments arranged by size: 

                1797    1432     299
 
XmnI GAAnn'nnTTC

Cuts at:      0    1317    3528 
   Size:       1317    2211
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     BanI     BbsI    BciVI    BfrBI     BmrI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BsrGI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     DraI     DrdI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII     KpnI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI    PflMI    PpuMI
    PshAI     PsiI     PstI    PvuII    SexAI     SfcI     SmlI    SnaBI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BsiEI    BsiWI    BspEI    BsrBI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BtgZI     ClaI    CspCI   DraIII
     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SpeI     SrfI     StuI     SwaI     XbaI
     XhoI     XmaI     ZraI
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