Restriction map for pF1KA1299
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccII       AclI        AcsI        AflII       
AgeI        AlwI        AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BalI        BamHI       BbrPI       BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FspI        HgaI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MscI        MunI        MvnI        NcoI        
NheI        NotI        NruI        NsiI        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     Van91I      XbaI        XcmI        
XhoII       


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         982  GACGTC               GACGT^C
AccI         1250  GTCTAC               GT^[AC][GT]AC
AfaI          928  GTAC                 GT^AC
AflIII       1025  ACACGT               A^C[GA][CT]GT
AhdI         1294  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1816  GTGCAC               G^TGCAC
Aor51HI       452  AGCGCT               AGC^GCT
ApaLI        1816  GTGCAC               G^TGCAC
Asp700I      1396  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1294  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          712  GGCGCC               GGCGC^C
BbsI         1651  GTCTTC               GAAGAC(2/6)
BbvCI         772  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1572  GTATCC               GTATCC(6/5)
BfaI           71  CTAG                 C^TAG
BlpI          205  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      205  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1651  GTCTTC               GAAGAC(2/6)
BsaI          556  GAGACC               GGTCTC(1/5)
BsmBI          41  CGTCTC               CGTCTC(1/5)
BsmI         1117  GAATGC               GAATGC(1/-1)
BsrFI         388  GCCGGC               [GA]^CCGG[CT]
BstAPI        959  GCATCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         881  TTCGAA               TT^CGAA
CelII         205  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        388  GCCGGC               [GA]^CCGG[CT]
DpnII         371  GATC                 ^GATC
EaeI          386  TGGCCG               [CT]^GGCC[GA]
Eam1105I     1294  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      452  AGCGCT               AGC^GCT
FseI          387  GGCCGGCC             GGCCGG^CC
HincII        526  GTTGAC               GT[CT]^[GA]AC
HindII        526  GTTGAC               GT[CT]^[GA]AC
KasI          712  GGCGCC               G^GCGCC
MaeI           71  CTAG                 C^TAG
MboI          371  GATC                 ^GATC
MseI         1037  TTAA                 T^TAA
NaeI          388  GCCGGC               GCC^GGC
NarI          712  GGCGCC               GG^CGCC
NdeI         1313  CATATG               CA^TATG
NdeII         371  GATC                 ^GATC
NgoMIV        388  GCCGGC               G^CCGGC
NspI         1086  GCATGT               [GA]CATG^[CT]
NspV          881  TTCGAA               TT^CGAA
PaeR7I       1801  CTCGAG               C^TCGAG
RsaI          928  GTAC                 GT^AC
SacI          208  GAGCTC               GAGCT^C
Sau3AI        371  GATC                 ^GATC
SfoI          712  GGCGCC               GGC^GCC
SfuI          881  TTCGAA               TT^CGAA
TfiI         1236  GAATC                G^A[AT]TC
Tru9I        1037  TTAA                 T^TAA
Tsp45I       1988  GTGAC                ^GT[GC]AC
XhoI         1801  CTCGAG               C^TCGAG
XmnI         1396  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccIII        986  TCCGGA               T^CCGGA
             1497  TCCGGA              
AcyI          712  GGCGCC               G[GA]^CG[CT]C
              982  GACGTC              
AspI          558  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              695  GACTCAGTC           
BanI            7  GGTGCC               G^G[CT][GA]CC
              712  GGCGCC              
BcgI         1391  CGATGGAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              861  GCAGAAGTGTCG        
BglI          232  GCCGAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              852  GCCTCAGTGGC         
BsaHI         712  GGCGCC               G[GA]^CG[CT]C
              982  GACGTC              
BsaWI         986  TCCGGA               [AT]^CCGG[AT]
             1497  TCCGGA              
BseAI         986  TCCGGA               T^CCGGA
             1497  TCCGGA              
BspEI         986  TCCGGA               T^CCGGA
             1497  TCCGGA              
BspMI        1265  ACCTGC               ACCTGC(4/8)
             1727  ACCTGC              
BsrDI        1368  CATTGC               GCAATG(2/0)
             1289  GCAATG              
BstXI         995  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1823  CCATCCCTTTGG        
HaeII         452  AGCGCT               [GA]GCGC^[CT]
              712  GGCGCC              
Hin1I         712  GGCGCC               G[GA]^CG[CT]C
              982  GACGTC              
MaeIII       1988  GTGAC                ^GT[ACGT]AC
             1996  GTAAC               
MroI          986  TCCGGA               T^CCGGA
             1497  TCCGGA              
MspA1I         49  CCGCTG               C[AC]G^C[GT]G
              542  CCGCTG              
PflFI         558  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              695  GACTCAGTC           
StuI          938  AGGCCT               AGG^CCT
             1094  AGGCCT              
Tth111I       558  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              695  GACTCAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
PpuMI       PstI        SapI        Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TseI        TspRI       TthHB8I     XmaCI       
XmaI        
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