Restriction map for
pF1KA1299
(Circular) (Six-base) MAPSORT of: pF1KA1299.seq Check: 7577 from: 1 to: 5126
pF1KA1299 5126 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1844 1844
Size: 5126
AatII G_ACGT'C
Cuts at: 1094 1094
Size: 5126
AccI GT'mk_AC
Cuts at: 1359 2164 1359
Size: 805 4321
Acc65I G'GTAC_C
Cuts at: 2142 2142
Size: 5126
AclI AA'CG_TT
Cuts at: 4966 4966
Size: 5126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 2951 3383 4130 4398 926
Size: 2025 432 747 268 1654
Fragments arranged by size:
2025 1654 747 432 268
AfeI AGC'GCT
Cuts at: 562 562
Size: 5126
AflIII A'CryG_T
Cuts at: 1133 2478 3582 4559 1133
Size: 1345 1104 977 1700
Fragments arranged by size:
1700 1345 1104 977
AgeI A'CCGG_T
Cuts at: 3514 3514
Size: 5126
AhdI GACnn_n'nnGTC
Cuts at: 1407 1407
Size: 5126
AleI CACnn'nnGTG
Cuts at: 1138 1138
Size: 5126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2095 2095
Size: 5126
AlwNI CAG_nnn'CTG
Cuts at: 895 1374 1881 2431 3998 895
Size: 479 507 550 1567 2023
Fragments arranged by size:
2023 1567 550 507 479
ApaI G_GGCC'C
Cuts at: 281 455 472 659 281
Size: 174 17 187 4748
Fragments arranged by size:
4748 187 174 17
ApaLI G'TGCA_C
Cuts at: 1924 3896 1924
Size: 1972 3154
ApoI r'AATT_y
Cuts at: 2130 4479 4735 2130
Size: 2349 256 2521
Fragments arranged by size:
2521 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
AvaI C'yCGr_G
Cuts at: 209 280 350 1909 2146 209
Size: 71 70 1559 237 3189
Fragments arranged by size:
3189 1559 237 71 70
BamHI G'GATC_C
Cuts at: 2151 2151
Size: 5126
BanI G'GyrC_C
Cuts at: 115 820 2142 2556 2804 2839 115
Size: 705 1322 414 248 35 2402
Fragments arranged by size:
2402 1322 705 414 248 35
BanII G_rGCy'C
Cuts at: 281 320 397 455 472 659 1237 2140 3170
Size: 39 77 58 17 187 578 903 1030
Cuts at: 3170 281
Size: 2237
Fragments arranged by size:
2237 1030 903 578 187 77 58 39
17
BbeI G_GCGC'C
Cuts at: 824 2560 2808 824
Size: 1736 248 3142
Fragments arranged by size:
3142 1736 248
BbsI GAAGACnn'nnnn_
Cuts at: 1752 1752
Size: 5126
BbvCI CC'TCA_GC
Cuts at: 881 881
Size: 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 958 992 1488 1522 958
Size: 34 496 34 4562
Fragments arranged by size:
4562 496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1691 3024 3785 5126 1691
Size: 1333 761 1341 1691
Fragments arranged by size:
1691 1341 1333 761
BclI T'GATC_A
Cuts at: 2646 2646
Size: 5126
BglI GCCn_nnn'nGGC
Cuts at: 346 966 346
Size: 620 4506
BglII A'GATC_T
Cuts at: 2641 4678 2641
Size: 2037 3089
BlpI GC'TnA_GC
Cuts at: 314 2238 314
Size: 1924 3202
Bme1580I G_kGCm'C
Cuts at: 120 281 455 472 659 1928 2751 2844 3900
Size: 161 174 17 187 1269 823 93 1056
Cuts at: 3900 120
Size: 1346
Fragments arranged by size:
1346 1269 1056 823 187 174 161 93
17
BmrI ACTGGGnnnn_n'
Cuts at: 676 2113 2338 2419 2754 4898 676
Size: 1437 225 81 335 2144 904
Fragments arranged by size:
2144 1437 904 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1045 1132 1348 1938 682
Size: 363 87 216 590 3870
Fragments arranged by size:
3870 590 363 216 87
Bpu10I CC'TnA_GC
Cuts at: 391 881 1231 391
Size: 490 350 4286
Fragments arranged by size:
4286 490 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 538 1696 2302 3181 3673 3971 451
Size: 87 1158 606 879 492 298 1606
Fragments arranged by size:
1606 1158 879 606 492 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 658 4830 658
Size: 4172 954
BsaAI yAC'GTr
Cuts at: 2479 2479
Size: 5126
BsaBI GATnn'nnATC
Cuts at: 6 2640 4677 6
Size: 2634 2037 455
Fragments arranged by size:
2634 2037 455
BsaHI Gr'CG_yC
Cuts at: 821 1091 2557 2805 3507 5003 821
Size: 270 1466 248 702 1496 944
Fragments arranged by size:
1496 1466 944 702 270 248
BsaWI w'CCGG_w
Cuts at: 1094 1605 2315 2836 3514 3788 3935 4346 1094
Size: 511 710 521 678 274 147 411 1874
Fragments arranged by size:
1874 710 678 521 511 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 241 271 628 655 658 685 688
Size: 30 87 30 357 27 3 27 3
Cuts at: 688 694 718 724 911 941 1120 1150 1849
Size: 6 24 6 187 30 179 30 699
Cuts at: 1849 1879 124
Size: 30 3371
Fragments arranged by size:
3371 699 357 187 179 87 30 30
30 30 30 27 27 24 6 6
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 516 528 636 702 898 935 965 1022 1025
Size: 12 108 66 196 37 30 57 3
Cuts at: 1025 1870 516
Size: 845 3772
Fragments arranged by size:
3772 845 196 108 66 57 37 30
12 3
BseYI C'CCAG_C
Cuts at: 465 652 724 900 931 1190 1363 1387 1462
Size: 187 72 176 31 259 173 24 75
Cuts at: 1462 1991 2017 2551 3309 3886 4382 465
Size: 529 26 534 758 577 496 1209
Fragments arranged by size:
1209 758 577 534 529 496 259 187
176 173 75 72 31 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1703 2055 868
Size: 835 352 3939
Fragments arranged by size:
3939 835 352
BsiEI CG_ry'CG
Cuts at: 104 2668 2714 3922 104
Size: 2564 46 1208 1308
Fragments arranged by size:
2564 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 320 1916 1928 2140 2918 3108 3900 320
Size: 1596 12 212 778 190 792 1546
Fragments arranged by size:
1596 1546 792 778 212 190 12
BsmI GAATG_Cn'
Cuts at: 1231 1231
Size: 5126
BsmBI CGTCTCn'nnnn_
Cuts at: 155 155
Size: 5126
Bsp1286I G_dGCh'C
Cuts at: 120 281 320 397 455 472 659 1237 1916
Size: 161 39 77 58 17 187 578 679
Cuts at: 1916 1928 2140 2751 2844 2918 3108 3170 3900
Size: 12 212 611 93 74 190 62 730
Cuts at: 3900 120
Size: 1346
Fragments arranged by size:
1346 730 679 611 578 212 190 187
161 93 77 74 62 58 39 17
12
BspEI T'CCGG_A
Cuts at: 1094 1605 4346 1094
Size: 511 2741 1874
Fragments arranged by size:
2741 1874 511
BspHI T'CATG_A
Cuts at: 4302 4302
Size: 5126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1382 1844 2176 2390 2692 3073 1382
Size: 462 332 214 302 381 3435
Fragments arranged by size:
3435 462 381 332 302 214
BsrBI CCG'CTC
Cuts at: 50 3418 3472 4956 5121 50
Size: 3368 54 1484 165 55
Fragments arranged by size:
3368 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1404 1475 3038 1404
Size: 71 1563 3492
Fragments arranged by size:
3492 1563 71
BsrFI r'CCGG_y
Cuts at: 496 3124 3305 3514 496
Size: 2628 181 209 2108
Fragments arranged by size:
2628 2108 209 181
BssSI C'ACGA_G
Cuts at: 3397 3755 3397
Size: 358 4768
BstAPI GCAn_nnn'nTGC
Cuts at: 1073 2503 1073
Size: 1430 3696
BstBI TT'CG_AA
Cuts at: 990 3487 990
Size: 2497 2629
BstXI CCAn_nnnn'nTGG
Cuts at: 1110 1938 1110
Size: 828 4298
BstYI r'GATC_y
Cuts at: 1 2151 2618 2641 2976 3222 4234 4320 4332
Size: 2150 467 23 335 246 1012 86 12
Cuts at: 4332 4678 1
Size: 346 449
Fragments arranged by size:
2150 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 606 1692 1752 606
Size: 1086 60 3980
Fragments arranged by size:
3980 1086 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3076 3257 3420 3076
Size: 181 163 4782
Fragments arranged by size:
4782 181 163
BtsI GCAGTG_nn'
Cuts at: 625 2369 625
Size: 1744 3382
DraI TTT'AAA
Cuts at: 2125 2125
Size: 5126
DrdI GACnn_nn'nnGTC
Cuts at: 2832 3690 2832
Size: 858 4268
EaeI y'GGCC_r
Cuts at: 494 2711 2885 3276 3303 4648 494
Size: 2217 174 391 27 1345 972
Fragments arranged by size:
2217 1345 972 391 174 27
EagI C'GGCC_G
Cuts at: 2711 2711
Size: 5126
EarI CTCTTCn'nnn_
Cuts at: 325 525 851 1083 1538 3149 3359 325
Size: 200 326 232 455 1611 210 2092
Fragments arranged by size:
2092 1611 455 326 232 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3334 3644 3790 4936 3334
Size: 310 146 1146 3524
Fragments arranged by size:
3524 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 2138 318
Size: 1820 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 926 1045 1132 1348 1938 2951 3383 4130
Size: 244 119 87 216 590 1013 432 747
Cuts at: 4130 4398 682
Size: 268 1410
Fragments arranged by size:
1410 1013 747 590 432 268 244 216
119 87
EcoNI CCTnn'n_nnAGG
Cuts at: 941 1411 1633 941
Size: 470 222 4434
Fragments arranged by size:
4434 470 222
EcoO109I rG'GnC_Cy
Cuts at: 137 277 452 469 489 656 676 1240 1292
Size: 140 175 17 20 167 20 564 52
Cuts at: 1292 1371 1428 1549 1622 2265 137
Size: 79 57 121 73 643 2998
Fragments arranged by size:
2998 643 564 175 167 140 121 79
73 57 52 20 20 17
EcoRI G'AATT_C
Cuts at: 2130 2130
Size: 5126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 3145 579
Size: 2566 2560
FseI GG_CCGG'CC
Cuts at: 500 500
Size: 5126
FspI TGC'GCA
Cuts at: 2907 2907
Size: 5126
HaeII r_GCGC'y
Cuts at: 564 824 2560 2808 3830 4809 564
Size: 260 1736 248 1022 979 881
Fragments arranged by size:
1736 1022 979 881 260 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 587 619 628 655 660 687 745
Size: 32 431 32 9 27 5 27 58
Cuts at: 745 777 792 824 1052 1084 1263 1295 1393
Size: 32 15 32 228 32 179 32 98
Cuts at: 1393 1425 1429 1461 1554 1586 1689 1721 2968
Size: 32 4 32 93 32 103 32 1247
Cuts at: 2968 2998 3000 3030 124
Size: 30 2 30 2220
Fragments arranged by size:
2220 1247 431 228 179 103 98 93
58 32 32 32 32 32 32 32
32 32 32 30 30 27 27 15
9 5 4 2
HincII GTy'rAC
Cuts at: 636 2165 636
Size: 1529 3597
KasI G'GCGC_C
Cuts at: 820 2556 2804 820
Size: 1736 248 3142
Fragments arranged by size:
3142 1736 248
KpnI G_GTAC'C
Cuts at: 2146 2146
Size: 5126
MluI A'CGCG_T
Cuts at: 4559 4559
Size: 5126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 731 3797 3981 731
Size: 3066 184 1876
Fragments arranged by size:
3066 1876 184
MscI TGG'CCA
Cuts at: 2887 2887
Size: 5126
MslI CAynn'nnrTG
Cuts at: 1138 2060 2077 3115 3242 1138
Size: 922 17 1038 127 3022
Fragments arranged by size:
3022 1038 922 127 17
MspA1I CmG'CkG
Cuts at: 159 652 2237 2551 2911 3924 4169 159
Size: 493 1585 314 360 1013 245 1116
Fragments arranged by size:
1585 1116 1013 493 360 314 245
NaeI GCC'GGC
Cuts at: 498 3307 498
Size: 2809 2317
NarI GG'CG_CC
Cuts at: 821 2557 2805 821
Size: 1736 248 3142
Fragments arranged by size:
3142 1736 248
NdeI CA'TA_TG
Cuts at: 1422 1422
Size: 5126
NgoMIV G'CCGG_C
Cuts at: 496 3305 496
Size: 2809 2317
NspI r_CATG'y
Cuts at: 1198 2179 2679 3586 1198
Size: 981 500 907 2738
Fragments arranged by size:
2738 981 907 500
PasI CC'CwG_GG
Cuts at: 766 1288 766
Size: 522 4604
PciI A'CATG_T
Cuts at: 3582 3582
Size: 5126
PmeI GTTT'AAAC
Cuts at: 2125 2125
Size: 5126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 4265
Size: 32 5094
PpuMI rG'GwC_Cy
Cuts at: 489 1240 1292 1371 1549 1622 489
Size: 751 52 79 178 73 3993
Fragments arranged by size:
3993 751 178 79 73 52
PspOMI G'GGCC_C
Cuts at: 277 451 468 655 277
Size: 174 17 187 4748
Fragments arranged by size:
4748 187 174 17
PspXI vC'TCGA_Gb
Cuts at: 1909 1909
Size: 5126
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1672 1704 1672
Size: 32 5094
PstI C_TGCA'G
Cuts at: 329 442 620 2173 2858 329
Size: 113 178 1553 685 2597
Fragments arranged by size:
2597 1553 685 178 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5126
PvuII CAG'CTG
Cuts at: 2551 2911 2551
Size: 360 4766
RsrII CG'GwC_CG
Cuts at: 3321 3321
Size: 5126
SacI G_AGCT'C
Cuts at: 320 2140 320
Size: 1820 3306
SalI G'TCGA_C
Cuts at: 2163 2163
Size: 5126
SanDI GG'GwC_CC
Cuts at: 1549 1549
Size: 5126
SapI GCTCTTCn'nnn_
Cuts at: 325 851 1083 3149 3359 325
Size: 526 232 2066 210 2092
Fragments arranged by size:
2092 2066 526 232 210
SbfI CC_TGCA'GG
Cuts at: 2173 2173
Size: 5126
ScaI AGT'ACT
Cuts at: 4643 4643
Size: 5126
SfcI C'TryA_G
Cuts at: 33 325 438 616 2169 2854 3847 4038 33
Size: 292 113 178 1553 685 993 191 1121
Fragments arranged by size:
1553 1121 993 685 292 191 178 113
SfoI GGC'GCC
Cuts at: 822 2558 2806 822
Size: 1736 248 3142
Fragments arranged by size:
3142 1736 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
SmaI CCC'GGG
Cuts at: 211 282 352 2148 211
Size: 71 70 1796 3189
Fragments arranged by size:
3189 1796 71 70
SmlI C'TyrA_G
Cuts at: 430 553 1711 1909 2281 3196 3688 3950 430
Size: 123 1158 198 372 915 492 262 1606
Fragments arranged by size:
1606 1158 915 492 372 262 198 123
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5126
SphI G_CATG'C
Cuts at: 2179 2679 2179
Size: 500 4626
SrfI GCCC'GGGC
Cuts at: 211 352 211
Size: 141 4985
StuI AGG'CCT
Cuts at: 1048 1204 1048
Size: 156 4970
StyI C'CwwG_G
Cuts at: 1043 1595 1684 2260 2614 1043
Size: 552 89 576 354 3555
Fragments arranged by size:
3555 576 552 354 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 436 1056 1197 2709 2842 3301 3510 390
Size: 46 620 141 1512 133 459 209 2006
Fragments arranged by size:
2006 1512 620 459 209 141 133 46
TatI w'GTAC_w
Cuts at: 4641 4641
Size: 5126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 911 943 1849 1881 4643 4675 911
Size: 32 906 32 2762 32 1362
Fragments arranged by size:
2762 1362 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 669 806 2923 669
Size: 137 2117 2872
Fragments arranged by size:
2872 2117 137
XbaI T'CTAG_A
Cuts at: 2157 2157
Size: 5126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 2621
Size: 5126
XhoI C'TCGA_G
Cuts at: 1909 1909
Size: 5126
XmaI C'CCGG_G
Cuts at: 209 280 350 2146 209
Size: 71 70 1796 3189
Fragments arranged by size:
3189 1796 71 70
XmnI GAAnn'nnTTC
Cuts at: 1508 1508
Size: 5126
ZraI GAC'GTC
Cuts at: 1092 1092
Size: 5126
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BfrBI BmgBI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI SacII SexAI SfiI SgrAI SnaBI SspI SwaI