Restriction map for pF1KA1299
 (Linear) (Six-base) MAPSORT of: KIAA1299.seq  Check: 2722  from: 1  to: 2013



KIAA1299.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1736    2013 
   Size:       1736     277
 
AatII G_ACGT'C

Cuts at:      0     986    2013 
   Size:        986    1027
 
AccI GT'mk_AC

Cuts at:      0    1251    2013 
   Size:       1251     762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    2013 
   Size:        818    1195
 
AfeI AGC'GCT

Cuts at:      0     454    2013 
   Size:        454    1559
 
AflIII A'CryG_T

Cuts at:      0    1025    2013 
   Size:       1025     988
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1299    2013 
   Size:       1299     714
 
AleI CACnn'nnGTG

Cuts at:      0    1030    2013 
   Size:       1030     983
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1987    2013 
   Size:       1987      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0     787    1266    1773    2013 
   Size:        787     479     507     240

  Fragments arranged by size: 

                 787     507     479     240
 
ApaI G_GGCC'C

Cuts at:      0     173     347     364     551    2013 
   Size:        173     174      17     187    1462

  Fragments arranged by size: 

                1462     187     174     173      17
 
ApaLI G'TGCA_C

Cuts at:      0    1816    2013 
   Size:       1816     197
 
AvaI C'yCGr_G

Cuts at:      0     101     172     242    1801    2013 
   Size:        101      71      70    1559     212

  Fragments arranged by size: 

                1559     212     101      71      70
 
BanI G'GyrC_C

Cuts at:      0       7     712    2013 
   Size:          7     705    1301

  Fragments arranged by size: 

                1301     705       7
 
BanII G_rGCy'C

Cuts at:      0     173     212     289     347     364     551    1129    2013 
   Size:        173      39      77      58      17     187     578     884

  Fragments arranged by size: 

                 884     578     187     173      77      58      39      17
            
 
BbeI G_GCGC'C

Cuts at:      0     716    2013 
   Size:        716    1297
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1644    2013 
   Size:       1644     369
 
BbvCI CC'TCA_GC

Cuts at:      0     773    2013 
   Size:        773    1240
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     850     884    1380    1414    2013 
   Size:        850      34     496      34     599

  Fragments arranged by size: 

                 850     599     496      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1583    2013 
   Size:       1583     430
 
BglI GCCn_nnn'nGGC

Cuts at:      0     238     858    2013 
   Size:        238     620    1155

  Fragments arranged by size: 

                1155     620     238
 
BlpI GC'TnA_GC

Cuts at:      0     206    2013 
   Size:        206    1807
 
Bme1580I G_kGCm'C

Cuts at:      0      12     173     347     364     551    1820    2013 
   Size:         12     161     174      17     187    1269     193

  Fragments arranged by size: 

                1269     193     187     174     161      17      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     568    2013 
   Size:        568    1445
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     937    1024    1240    1830    2013 
   Size:        574     363      87     216     590     183

  Fragments arranged by size: 

                 590     574     363     216     183      87
 
Bpu10I CC'TnA_GC

Cuts at:      0     283     773    1123    2013 
   Size:        283     490     350     890

  Fragments arranged by size: 

                 890     490     350     283
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     430    1588    2013 
   Size:        343      87    1158     425

  Fragments arranged by size: 

                1158     425     343      87
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     550    2013 
   Size:        550    1463
 
BsaHI Gr'CG_yC

Cuts at:      0     713     983    2013 
   Size:        713     270    1030

  Fragments arranged by size: 

                1030     713     270
 
BsaWI w'CCGG_w

Cuts at:      0     986    1497    2013 
   Size:        986     511     516

  Fragments arranged by size: 

                 986     516     511
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      16      46     133     163     520     547     550     577 
   Size:         16      30      87      30     357      27       3      27

Cuts at:    577     580     586     610     616     803     833    1012    1042 
   Size:          3       6      24       6     187      30     179      30

Cuts at:   1042    1741    1771    2013 
   Size:        699      30     242

  Fragments arranged by size: 

                 699     357     242     187     179      87      30      30
                  30      30      30      27      27      24      16       6
                   6       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     408     420     528     594     790     827     857     914 
   Size:        408      12     108      66     196      37      30      57

Cuts at:    914     917    1762    2013 
   Size:          3     845     251

  Fragments arranged by size: 

                 845     408     251     196     108      66      57      37
                  30      12       3
 
BseYI C'CCAG_C

Cuts at:      0     357     544     616     792     823    1082    1255    1279 
   Size:        357     187      72     176      31     259     173      24

Cuts at:   1279    1354    1883    1909    2013 
   Size:         75     529      26     104

  Fragments arranged by size: 

                 529     357     259     187     176     173     104      75
                  72      31      26      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    1595    1947    2013 
   Size:        760     835     352      66

  Fragments arranged by size: 

                 835     760     352      66
 
BsiHKAI G_wGCw'C

Cuts at:      0     212    1808    1820    2013 
   Size:        212    1596      12     193

  Fragments arranged by size: 

                1596     212     193      12
 
BsmI GAATG_Cn'

Cuts at:      0    1123    2013 
   Size:       1123     890
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      47    2013 
   Size:         47    1966
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     173     212     289     347     364     551    1129 
   Size:         12     161      39      77      58      17     187     578

Cuts at:   1129    1808    1820    2013 
   Size:        679      12     193

  Fragments arranged by size: 

                 679     578     193     187     161      77      58      39
                  17      12      12
 
BspEI T'CCGG_A

Cuts at:      0     986    1497    2013 
   Size:        986     511     516

  Fragments arranged by size: 

                 986     516     511
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1274    1736    2013 
   Size:       1274     462     277

  Fragments arranged by size: 

                1274     462     277
 
BsrDI GCAATG_nn'

Cuts at:      0    1296    1367    2013 
   Size:       1296      71     646

  Fragments arranged by size: 

                1296     646      71
 
BsrFI r'CCGG_y

Cuts at:      0     388    2013 
   Size:        388    1625
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     965    2013 
   Size:        965    1048
 
BstBI TT'CG_AA

Cuts at:      0     882    2013 
   Size:        882    1131
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1002    1830    2013 
   Size:       1002     828     183

  Fragments arranged by size: 

                1002     828     183
 
BtgI C'CryG_G

Cuts at:      0     498    1584    1644    2013 
   Size:        498    1086      60     369

  Fragments arranged by size: 

                1086     498     369      60
 
BtsI GCAGTG_nn'

Cuts at:      0     517    2013 
   Size:        517    1496
 
EaeI y'GGCC_r

Cuts at:      0     386    2013 
   Size:        386    1627
 
EarI CTCTTCn'nnn_

Cuts at:      0     217     417     743     975    1430    2013 
   Size:        217     200     326     232     455     583

  Fragments arranged by size: 

                 583     455     326     232     217     200
 
EcoICRI GAG'CTC

Cuts at:      0     210    2013 
   Size:        210    1803
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     818     937    1024    1240    1830    2013 
   Size:        574     244     119      87     216     590     183

  Fragments arranged by size: 

                 590     574     244     216     183     119      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     833    1303    1525    2013 
   Size:        833     470     222     488

  Fragments arranged by size: 

                 833     488     470     222
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29     169     344     361     381     548     568    1132 
   Size:         29     140     175      17      20     167      20     564

Cuts at:   1132    1184    1263    1320    1441    1514    2013 
   Size:         52      79      57     121      73     499

  Fragments arranged by size: 

                 564     499     175     167     140     121      79      73
                  57      52      29      20      20      17
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     471    2013 
   Size:        471    1542
 
FseI GG_CCGG'CC

Cuts at:      0     392    2013 
   Size:        392    1621
 
HaeII r_GCGC'y

Cuts at:      0     456     716    2013 
   Size:        456     260    1297

  Fragments arranged by size: 

                1297     456     260
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     479     511     520     547     552     579 
   Size:         16      32     431      32       9      27       5      27

Cuts at:    579     637     669     684     716     944     976    1155    1187 
   Size:         58      32      15      32     228      32     179      32

Cuts at:   1187    1285    1317    1321    1353    1446    1478    1581    1613 
   Size:         98      32       4      32      93      32     103      32

Cuts at:   1613    2013 
   Size:        400

  Fragments arranged by size: 

                 431     400     228     179     103      98      93      58
                  32      32      32      32      32      32      32      32
                  32      32      27      27      16      15       9       5
                   4
 
HincII GTy'rAC

Cuts at:      0     528    2013 
   Size:        528    1485
 
KasI G'GCGC_C

Cuts at:      0     712    2013 
   Size:        712    1301
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    2013 
   Size:        623    1390
 
MslI CAynn'nnrTG

Cuts at:      0    1030    1952    1969    2013 
   Size:       1030     922      17      44

  Fragments arranged by size: 

                1030     922      44      17
 
MspA1I CmG'CkG

Cuts at:      0      51     544    2013 
   Size:         51     493    1469

  Fragments arranged by size: 

                1469     493      51
 
NaeI GCC'GGC

Cuts at:      0     390    2013 
   Size:        390    1623
 
NarI GG'CG_CC

Cuts at:      0     713    2013 
   Size:        713    1300
 
NdeI CA'TA_TG

Cuts at:      0    1314    2013 
   Size:       1314     699
 
NgoMIV G'CCGG_C

Cuts at:      0     388    2013 
   Size:        388    1625
 
NspI r_CATG'y

Cuts at:      0    1090    2013 
   Size:       1090     923
 
PasI CC'CwG_GG

Cuts at:      0     658    1180    2013 
   Size:        658     522     833

  Fragments arranged by size: 

                 833     658     522
 
PpuMI rG'GwC_Cy

Cuts at:      0     381    1132    1184    1263    1441    1514    2013 
   Size:        381     751      52      79     178      73     499

  Fragments arranged by size: 

                 751     499     381     178      79      73      52
 
PspOMI G'GGCC_C

Cuts at:      0     169     343     360     547    2013 
   Size:        169     174      17     187    1466

  Fragments arranged by size: 

                1466     187     174     169      17
 
PspXI vC'TCGA_Gb

Cuts at:      0    1801    2013 
   Size:       1801     212
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    2013 
   Size:       1564      32     417

  Fragments arranged by size: 

                1564     417      32
 
PstI C_TGCA'G

Cuts at:      0     221     334     512    2013 
   Size:        221     113     178    1501

  Fragments arranged by size: 

                1501     221     178     113
 
SacI G_AGCT'C

Cuts at:      0     212    2013 
   Size:        212    1801
 
SanDI GG'GwC_CC

Cuts at:      0    1441    2013 
   Size:       1441     572
 
SapI GCTCTTCn'nnn_

Cuts at:      0     217     743     975    2013 
   Size:        217     526     232    1038

  Fragments arranged by size: 

                1038     526     232     217
 
SfcI C'TryA_G

Cuts at:      0     217     330     508    2013 
   Size:        217     113     178    1505

  Fragments arranged by size: 

                1505     217     178     113
 
SfoI GGC'GCC

Cuts at:      0     714    2013 
   Size:        714    1299
 
SmaI CCC'GGG

Cuts at:      0     103     174     244    2013 
   Size:        103      71      70    1769

  Fragments arranged by size: 

                1769     103      71      70
 
SmlI C'TyrA_G

Cuts at:      0     322     445    1603    1801    2013 
   Size:        322     123    1158     198     212

  Fragments arranged by size: 

                1158     322     212     198     123
 
SrfI GCCC'GGGC

Cuts at:      0     103     244    2013 
   Size:        103     141    1769

  Fragments arranged by size: 

                1769     141     103
 
StuI AGG'CCT

Cuts at:      0     940    1096    2013 
   Size:        940     156     917

  Fragments arranged by size: 

                 940     917     156
 
StyI C'CwwG_G

Cuts at:      0     935    1487    1576    2013 
   Size:        935     552      89     437

  Fragments arranged by size: 

                 935     552     437      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     282     328     948    1089    2013 
   Size:        282      46     620     141     924

  Fragments arranged by size: 

                 924     620     282     141      46
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     803     835    1741    1773    2013 
   Size:        803      32     906      32     240

  Fragments arranged by size: 

                 906     803     240      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     561     698    2013 
   Size:        561     137    1315

  Fragments arranged by size: 

                1315     561     137
 
XhoI C'TCGA_G

Cuts at:      0    1801    2013 
   Size:       1801     212
 
XmaI C'CCGG_G

Cuts at:      0     101     172     242    2013 
   Size:        101      71      70    1771

  Fragments arranged by size: 

                1771     101      71      70
 
XmnI GAAnn'nnTTC

Cuts at:      0    1400    2013 
   Size:       1400     613
 
ZraI GAC'GTC

Cuts at:      0     984    2013 
   Size:        984    1029
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI   BstAPI    BstBI    BstXI     BtgI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI    HaeII
    Hin4I   HincII     KasI     MmeI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NspI     PasI    PpuMI   PspOMI    PspXI     PsrI
     PstI     SacI    SanDI     SapI     SfcI     SfoI     SmaI     SmlI
     SrfI     StuI     StyI    TaqII     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BclI    BfrBI    BglII    BmgBI
     BmtI     BplI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrBI
    BsrGI   BssHII    BssSI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NcoI     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PvuI
    PvuII    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI     TatI
     XbaI     XcmI
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