Restriction map for pF1KA1221
 (Linear) (Six-base) MAPSORT of: KIAA1221.seq  Check: 8717  from: 1  to: 3981



KIAA1221.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1203    3981 
   Size:       1203    2778
 
AccI GT'mk_AC

Cuts at:      0     490     968    1004    3981 
   Size:        490     478      36    2977

  Fragments arranged by size: 

                2977     490     478      36
 
Acc65I G'GTAC_C

Cuts at:      0    1510    3981 
   Size:       1510    2471
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     466     578    1444    1769    3422    3981 
   Size:        245     221     112     866     325    1653     559

  Fragments arranged by size: 

                1653     866     559     325     245     221     112
 
AflII C'TTAA_G

Cuts at:      0     258    3981 
   Size:        258    3723
 
AflIII A'CryG_T

Cuts at:      0    2090    2412    3981 
   Size:       2090     322    1569

  Fragments arranged by size: 

                2090    1569     322
 
AleI CACnn'nnGTG

Cuts at:      0    2411    3981 
   Size:       2411    1570
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3418    3981 
   Size:       3418     563
 
ApaLI G'TGCA_C

Cuts at:      0    1478    3981 
   Size:       1478    2503
 
ApoI r'AATT_y

Cuts at:      0     615     728    1251    1439    2027    2307    2661    3441 
   Size:        615     113     523     188     588     280     354     780

Cuts at:   3441    3552    3981 
   Size:        111     429

  Fragments arranged by size: 

                 780     615     588     523     429     354     280     188
                 113     111
 
AseI AT'TA_AT

Cuts at:      0    2645    3981 
   Size:       2645    1336
 
BamHI G'GATC_C

Cuts at:      0     777    3981 
   Size:        777    3204
 
BanI G'GyrC_C

Cuts at:      0    1510    3981 
   Size:       1510    2471
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1061    1425    2477    3839    3981 
   Size:       1061     364    1052    1362     142

  Fragments arranged by size: 

                1362    1061    1052     364     142
 
BbvCI CC'TCA_GC

Cuts at:      0    2807    3981 
   Size:       2807    1174
 
BclI T'GATC_A

Cuts at:      0    2246    3981 
   Size:       2246    1735
 
BfrBI ATG'CAT

Cuts at:      0    1021    3981 
   Size:       1021    2960
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1631    3981 
   Size:       1631    2350
 
BglII A'GATC_T

Cuts at:      0    3721    3981 
   Size:       3721     260
 
Bme1580I G_kGCm'C

Cuts at:      0    1482    3981 
   Size:       1482    2499
 
BmgBI CAC'GTC

Cuts at:      0    2413    3911    3981 
   Size:       2413    1498      70

  Fragments arranged by size: 

                2413    1498      70
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     783    3476    3981 
   Size:        783    2693     505

  Fragments arranged by size: 

                2693     783     505
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2726    3908    3981 
   Size:       2726    1182      73

  Fragments arranged by size: 

                2726    1182      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    2807    3981 
   Size:       2807    1174
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2899    3981 
   Size:       2899    1082
 
BsaBI GATnn'nnATC

Cuts at:      0    2483    2645    3981 
   Size:       2483     162    1336

  Fragments arranged by size: 

                2483    1336     162
 
BsaHI Gr'CG_yC

Cuts at:      0    2378    3981 
   Size:       2378    1603
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359    3981 
   Size:        329      30    3622

  Fragments arranged by size: 

                3622     329      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     357    2102    3557    3981 
   Size:        357    1745    1455     424

  Fragments arranged by size: 

                1745    1455     424     357
 
BseYI C'CCAG_C

Cuts at:      0    2904    3981 
   Size:       2904    1077
 
BsiHKAI G_wGCw'C

Cuts at:      0     300    1482    3981 
   Size:        300    1182    2499

  Fragments arranged by size: 

                2499    1182     300
 
BsmI GAATG_Cn'

Cuts at:      0    1627    1824    3981 
   Size:       1627     197    2157

  Fragments arranged by size: 

                2157    1627     197
 
Bsp1286I G_dGCh'C

Cuts at:      0     300    1482    3981 
   Size:        300    1182    2499

  Fragments arranged by size: 

                2499    1182     300
 
BspHI T'CATG_A

Cuts at:      0    1712    3981 
   Size:       1712    2269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1203    3779    3981 
   Size:       1203    2576     202

  Fragments arranged by size: 

                2576    1203     202
 
BsrBI CCG'CTC

Cuts at:      0     161    3981 
   Size:        161    3820
 
BsrDI GCAATG_nn'

Cuts at:      0    1251    1634    2069    3981 
   Size:       1251     383     435    1912

  Fragments arranged by size: 

                1912    1251     435     383
 
BsrGI T'GTAC_A

Cuts at:      0    1234    3981 
   Size:       1234    2747
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      75    2740    3981 
   Size:         75    2665    1241

  Fragments arranged by size: 

                2665    1241      75
 
BstYI r'GATC_y

Cuts at:      0     777    3575    3721    3981 
   Size:        777    2798     146     260

  Fragments arranged by size: 

                2798     777     260     146
 
Bsu36I CC'TnA_GG

Cuts at:      0     735    3981 
   Size:        735    3246
 
BtgI C'CryG_G

Cuts at:      0    3880    3981 
   Size:       3880     101
 
BtsI GCAGTG_nn'

Cuts at:      0     408    1607    1655    3981 
   Size:        408    1199      48    2326

  Fragments arranged by size: 

                2326    1199     408      48
 
DraI TTT'AAA

Cuts at:      0    1802    1970    3981 
   Size:       1802     168    2011

  Fragments arranged by size: 

                2011    1802     168
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2871    3981 
   Size:       2871    1110
 
EaeI y'GGCC_r

Cuts at:      0    2038    3965    3981 
   Size:       2038    1927      16

  Fragments arranged by size: 

                2038    1927      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     983    1109    1169    1208    1577    3981 
   Size:        983     126      60      39     369    2404

  Fragments arranged by size: 

                2404     983     369     126      60      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     466     578    1444    1769    2726    3422    3908 
   Size:        245     221     112     866     325     957     696     486

Cuts at:   3908    3981 
   Size:         73

  Fragments arranged by size: 

                 957     866     696     486     325     245     221     112
                  73
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3814    3981 
   Size:       3814     167
 
EcoRI G'AATT_C

Cuts at:      0     615    3552    3981 
   Size:        615    2937     429

  Fragments arranged by size: 

                2937     615     429
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1048    1080    1174    1206    2787    2819    2872    2904 
   Size:       1048      32      94      32    1581      32      53      32

Cuts at:   2904    3337    3369    3390    3422    3567    3599    3981 
   Size:        433      32      21      32     145      32     382

  Fragments arranged by size: 

                1581    1048     433     382     145      94      53      32
                  32      32      32      32      32      32      21
 
HincII GTy'rAC

Cuts at:      0     215     511    1943    2145    3981 
   Size:        215     296    1432     202    1836

  Fragments arranged by size: 

                1836    1432     296     215     202
 
HindIII A'AGCT_T

Cuts at:      0    2394    3981 
   Size:       2394    1587
 
KpnI G_GTAC'C

Cuts at:      0    1514    3981 
   Size:       1514    2467
 
MfeI C'AATT_G

Cuts at:      0    1515    3981 
   Size:       1515    2466
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1104    2442    3981 
   Size:       1104    1338    1539

  Fragments arranged by size: 

                1539    1338    1104
 
MslI CAynn'nnrTG

Cuts at:      0     293    1298    1325    1600    1833    2120    2228    2411 
   Size:        293    1005      27     275     233     287     108     183

Cuts at:   2411    3981 
   Size:       1570

  Fragments arranged by size: 

                1570    1005     293     287     275     233     183     108
                  27
 
MspA1I CmG'CkG

Cuts at:      0     475     486    3981 
   Size:        475      11    3495

  Fragments arranged by size: 

                3495     475      11
 
NdeI CA'TA_TG

Cuts at:      0    1283    1341    1397    1759    3981 
   Size:       1283      58      56     362    2222

  Fragments arranged by size: 

                2222    1283     362      58      56
 
NsiI A_TGCA'T

Cuts at:      0    1023    3981 
   Size:       1023    2958
 
NspI r_CATG'y

Cuts at:      0     292     550    1543    2094    2659    3981 
   Size:        292     258     993     551     565    1322

  Fragments arranged by size: 

                1322     993     565     551     292     258
 
PciI A'CATG_T

Cuts at:      0    2090    3981 
   Size:       2090    1891
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3886    3981 
   Size:       3886      95
 
PpuMI rG'GwC_Cy

Cuts at:      0    3814    3981 
   Size:       3814     167
 
PshAI GACnn'nnGTC

Cuts at:      0    2512    3819    3981 
   Size:       2512    1307     162

  Fragments arranged by size: 

                2512    1307     162
 
PsiI TTA'TAA

Cuts at:      0    1094    3981 
   Size:       1094    2887
 
PstI C_TGCA'G

Cuts at:      0    2826    3981 
   Size:       2826    1155
 
PvuII CAG'CTG

Cuts at:      0     475     486    3981 
   Size:        475      11    3495

  Fragments arranged by size: 

                3495     475      11
 
SapI GCTCTTCn'nnn_

Cuts at:      0     983    1208    3981 
   Size:        983     225    2773

  Fragments arranged by size: 

                2773     983     225
 
ScaI AGT'ACT

Cuts at:      0    1819    3981 
   Size:       1819    2162
 
SfcI C'TryA_G

Cuts at:      0    1724    1901    2238    2634    2822    3083    3981 
   Size:       1724     177     337     396     188     261     898

  Fragments arranged by size: 

                1724     898     396     337     261     188     177
 
SmlI C'TyrA_G

Cuts at:      0     258    2878    3981 
   Size:        258    2620    1103

  Fragments arranged by size: 

                2620    1103     258
 
StuI AGG'CCT

Cuts at:      0    2388    3981 
   Size:       2388    1593
 
StyI C'CwwG_G

Cuts at:      0    1333    2195    3981 
   Size:       1333     862    1786

  Fragments arranged by size: 

                1786    1333     862
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     577     661    3981 
   Size:        577      84    3320

  Fragments arranged by size: 

                3320     577      84
 
TatI w'GTAC_w

Cuts at:      0    1234    1817    3981 
   Size:       1234     583    2164

  Fragments arranged by size: 

                2164    1234     583
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     413     445    3981 
   Size:        413      32    3536

  Fragments arranged by size: 

                3536     413      32
 
XbaI T'CTAG_A

Cuts at:      0      40     858     975    3981 
   Size:         40     818     117    3006

  Fragments arranged by size: 

                3006     818     117      40
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AleI    AlwNI
    ApaLI     ApoI     AseI    BamHI     BanI     BbsI    BbvCI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI    PpuMI
    PshAI     PsiI     PstI    PvuII     SapI     ScaI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AhdI     AlfI     AloI     ApaI
     AscI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BcgI
    BciVI     BlpI     BmtI     BplI     BsaI    BsaAI    BsaWI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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