Restriction map for pF1KA1114
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AhdI        AocI        AscI        
AseI        AsnI        Asp700I     AspEI       BbeI        
BbrPI       BcgI        BclI        BfrI        BsaAI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoRI       FbaI        FseI        
Hin1I       HpaI        KasI        KspI        MluI        
MroI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
SspBI       SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       3730  GGTACC               G^GTACC
AcsI          930  AAATTC               [GA]^AATT[CT]
AgeI         3583  ACCGGT               A^CCGGT
Aor51HI      3736  AGCGCT               AGC^GCT
ApoI          930  AAATTC               [GA]^AATT[CT]
Asp718I      3730  GGTACC               G^GTACC
AspI          118  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1676  TGCGCA               TGC^GCA
AvrII        3666  CCTAGG               C^CTAGG
BamHI        1283  GGATCC               G^GATCC
BciVI         731  GTATCC               GTATCC(6/5)
BglII         944  AGATCT               A^GATCT
BlnI         3666  CCTAGG               C^CTAGG
BsaWI        3583  ACCGGT               [AT]^CCGG[AT]
BsmI         1707  GCATTC               GAATGC(1/-1)
BsrDI        3599  GCAATG               GCAATG(2/0)
BssSI        1837  CACGAG               CACGAG(-5/-1)
BstAPI        247  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        369  GGTAACC              G^GT[ACGT]ACC
BstPI         369  GGTAACC              G^GT[ACGT]ACC
BstXI         474  CCATGAGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          318  TTTAAA               TTT^AAA
Eco47III     3736  AGCGCT               AGC^GCT
EcoO65I       369  GGTAACC              G^GT[ACGT]ACC
EcoRV        2050  GATATC               GAT^ATC
EcoT22I       133  ATGCAT               ATGCA^T
FauI         1005  GCGGG                CCCGC(4/6)
FspI         1676  TGCGCA               TGC^GCA
HaeII        3736  AGCGCT               [GA]GCGC^[CT]
HindIII       611  AAGCTT               A^AGCTT
KpnI         3730  GGTACC               GGTAC^C
NsiI          133  ATGCAT               ATGCA^T
PflFI         118  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3583  ACCGGT               A^CCGGT
SacI         3452  GAGCTC               GAGCT^C
SapI         2822  GCTCTTC              GCTCTTC(1/4)
ScaI         2956  AGTACT               AGT^ACT
Tth111I       118  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII        1313  CGCG                 CG^CG
             1918  CGCG                
BbsI          783  GAAGAC               GAAGAC(2/6)
             2632  GTCTTC              
BglI         1039  GCCACTAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1648  GCCAGTGAGGC         
BlpI          400  GCTCAGC              GC^T[ACGT]AGC
             2646  GCTTAGC             
Bpu1102I      400  GCTCAGC              GC^T[ACGT]AGC
             2646  GCTTAGC             
BpuAI         783  GAAGAC               GAAGAC(2/6)
             2632  GTCTTC              
BsaBI        1171  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1386  GATCCCCATC          
BseRI        1603  CTCCTC               GAGGAG(10/8)
             2174  GAGGAG              
BsmFI         265  GTCCC                GGGAC(10/14)
             1783  GTCCC               
BsrFI         191  ACCGGC               [GA]^CCGG[CT]
             3583  ACCGGT              
BstUI        1313  CGCG                 CG^CG
             1918  CGCG                
CelII         400  GCTCAGC              GC^T[ACGT]AGC
             2646  GCTTAGC             
Cfr10I        191  ACCGGC               [GA]^CCGG[CT]
             3583  ACCGGT              
HgaI          844  GACGC                GACGC(5/10)
             3137  GCGTC               
HincII        187  GTCAAC               GT[CT]^[GA]AC
             2161  GTCAAC              
HindII        187  GTCAAC               GT[CT]^[GA]AC
             2161  GTCAAC              
MaeII        1121  ACGT                 A^CGT
             2159  ACGT                
MamI         1171  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1386  GATCCCCATC          
MfeI          554  CAATTG               C^AATTG
              788  CAATTG              
MunI          554  CAATTG               C^AATTG
              788  CAATTG              
MvnI         1313  CGCG                 CG^CG
             1918  CGCG                
NcoI          170  CCATGG               C^CATGG
             1288  CCATGG              
SmaI           60  CCCGGG               CCC^GGG
             1214  CCCGGG              
SmlI         1132  CTTGAG               C^T[CT][GA]AG
             1861  CTCAAG              
SpeI         2407  ACTAGT               A^CTAGT
             3853  ACTAGT              
SphI         1870  GCATGC               GCATG^C
             2444  GCATGC              
SspI          541  AATATT               AAT^ATT
             1439  AATATT              
TfiI         1552  GAATC                G^A[AT]TC
             1614  GATTC               
XmaCI          60  CCCGGG               C^CCGGG
             1214  CCCGGG              
XmaI           60  CCCGGG               C^CCGGG
             1214  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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