Restriction map for pF1KA1114
 (Linear) (Six-base) MAPSORT of: KIAA1114.seq  Check: 8668  from: 1  to: 4293



KIAA1114.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     258    4293 
   Size:        258    4035
 
Acc65I G'GTAC_C

Cuts at:      0    3730    4293 
   Size:       3730     563
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     517    1991    2151    2181    2235    2289    2343 
   Size:        374     143    1474     160      30      54      54      54

Cuts at:   2343    2619    3219    3249    3669    3789    4053    4083    4140 
   Size:        276     600      30     420     120     264      30      57

Cuts at:   4140    4293 
   Size:        153

  Fragments arranged by size: 

                1474     600     420     374     276     264     160     153
                 143     120      57      54      54      54      30      30
                  30
 
AfeI AGC'GCT

Cuts at:      0    3738    4293 
   Size:       3738     555
 
AgeI A'CCGG_T

Cuts at:      0    3583    4293 
   Size:       3583     710
 
AlwNI CAG_nnn'CTG

Cuts at:      0     397    1164    2690    2750    2870    3020    3140    3440 
   Size:        397     767    1526      60     120     150     120     300

Cuts at:   3440    3470    3530    4180    4293 
   Size:         30      60     650     113

  Fragments arranged by size: 

                1526     767     650     397     300     150     120     120
                 113      60      60      30
 
ApaI G_GGCC'C

Cuts at:      0      49     928    1092    2030    4293 
   Size:         49     879     164     938    2263

  Fragments arranged by size: 

                2263     938     879     164      49
 
ApaLI G'TGCA_C

Cuts at:      0    2228    2390    2612    3062    3182    3272    4293 
   Size:       2228     162     222     450     120      90    1021

  Fragments arranged by size: 

                2228    1021     450     222     162     120      90
 
ApoI r'AATT_y

Cuts at:      0     930    4293 
   Size:        930    3363
 
AvaI C'yCGr_G

Cuts at:      0      60    1049    1214    4293 
   Size:         60     989     165    3079

  Fragments arranged by size: 

                3079     989     165      60
 
AvrII C'CTAG_G

Cuts at:      0    3666    4293 
   Size:       3666     627
 
BamHI G'GATC_C

Cuts at:      0    1283    4293 
   Size:       1283    3010
 
BanI G'GyrC_C

Cuts at:      0      30    3040    3430    3670    3730    3760    3790    3820 
   Size:         30    3010     390     240      60      30      30      30

Cuts at:   3820    3880    3910    4270    4293 
   Size:         60      30     360      23

  Fragments arranged by size: 

                3010     390     360     240      60      60      30      30
                  30      30      30      23
 
BanII G_rGCy'C

Cuts at:      0      49     928    1029    1092    1202    1995    2030    2133 
   Size:         49     879     101      63     110     793      35     103

Cuts at:   2133    3456    4293 
   Size:       1323     837

  Fragments arranged by size: 

                1323     879     837     793     110     103     101      63
                  49      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     790    2625    4293 
   Size:        790    1835    1668

  Fragments arranged by size: 

                1835    1668     790
 
BbvCI CC'TCA_GC

Cuts at:      0    1411    1964    1982    3937    4293 
   Size:       1411     553      18    1955     356

  Fragments arranged by size: 

                1955    1411     553     356      18
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     742    4293 
   Size:        742    3551
 
BfrBI ATG'CAT

Cuts at:      0     135    4293 
   Size:        135    4158
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1045    1654    4293 
   Size:       1045     609    2639

  Fragments arranged by size: 

                2639    1045     609
 
BglII A'GATC_T

Cuts at:      0     944    4293 
   Size:        944    3349
 
BlpI GC'TnA_GC

Cuts at:      0     401    2647    4293 
   Size:        401    2246    1646

  Fragments arranged by size: 

                2246    1646     401
 
Bme1580I G_kGCm'C

Cuts at:      0      49     928    1092    1131    2030    2232    2394    2616 
   Size:         49     879     164      39     899     202     162     222

Cuts at:   2616    3066    3186    3276    3336    3793    3823    3883    4293 
   Size:        450     120      90      60     457      30      60     410

  Fragments arranged by size: 

                 899     879     457     450     410     222     202     164
                 162     120      90      60      60      49      39      30
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1579    1911    3796    3826    3886    4076    4293 
   Size:       1579     332    1885      30      60     190     217

  Fragments arranged by size: 

                1885    1579     332     217     190      60      30
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2572    2692    4293 
   Size:       2572     120    1601

  Fragments arranged by size: 

                2572    1601     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     245    1216    1498    1789    2118    2223    2337 
   Size:         71     174     971     282     291     329     105     114

Cuts at:   2337    2697    4293 
   Size:        360    1596

  Fragments arranged by size: 

                1596     971     360     329     291     282     174     114
                 105      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     794    1411    1964    1982    2090    3697    3937    4293 
   Size:        794     617     553      18     108    1607     240     356

  Fragments arranged by size: 

                1607     794     617     553     356     240     108      18
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1153    1846    4293 
   Size:       1153     693    2447

  Fragments arranged by size: 

                2447    1153     693
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      97     669    1606    4293 
   Size:         97     572     937    2687

  Fragments arranged by size: 

                2687     937     572      97
 
BsaBI GATnn'nnATC

Cuts at:      0    1175    1390    4293 
   Size:       1175     215    2903

  Fragments arranged by size: 

                2903    1175     215
 
BsaWI w'CCGG_w

Cuts at:      0    3583    4293 
   Size:       3583     710
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1471    1501    4293 
   Size:       1471      30    2792

  Fragments arranged by size: 

                2792    1471      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1594    2189    4293 
   Size:       1594     595    2104

  Fragments arranged by size: 

                2104    1594     595
 
BseYI C'CCAG_C

Cuts at:      0     267    1596    2023    2233    2287    2707    2887    3157 
   Size:        267    1329     427     210      54     420     180     270

Cuts at:   3157    3367    3397    3457    3571    4051    4293 
   Size:        210      30      60     114     480     242

  Fragments arranged by size: 

                1329     480     427     420     270     267     242     210
                 210     180     114      60      54      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230    1289    1900    1930    2754    4293 
   Size:        230    1059     611      30     824    1539

  Fragments arranged by size: 

                1539    1059     824     611     230      30
 
BsiHKAI G_wGCw'C

Cuts at:      0    2232    2347    2394    2616    2629    2916    2948    2983 
   Size:       2232     115      47     222      13     287      32      35

Cuts at:   2983    3066    3186    3276    3426    3456    4174    4234    4293 
   Size:         83     120      90     150      30     718      60      59

  Fragments arranged by size: 

                2232     718     287     222     150     120     115      90
                  83      60      59      47      35      32      30      13
            
 
BsmI GAATG_Cn'

Cuts at:      0    1707    4293 
   Size:       1707    2586
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     928    1029    1092    1131    1202    1995    2030 
   Size:         49     879     101      63      39      71     793      35

Cuts at:   2030    2133    2232    2347    2394    2616    2629    2916    2948 
   Size:        103      99     115      47     222      13     287      32

Cuts at:   2948    2983    3066    3186    3276    3336    3426    3456    3793 
   Size:         35      83     120      90      60      90      30     337

Cuts at:   3793    3823    3883    4174    4234    4293 
   Size:         30      60     291      60      59

  Fragments arranged by size: 

                 879     793     337     291     287     222     120     115
                 103     101      99      90      90      83      71      63
                  60      60      60      59      49      47      39      35
                  35      32      30      30      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     258     332     501    4293 
   Size:        258      74     169    3792

  Fragments arranged by size: 

                3792     258     169      74
 
BsrDI GCAATG_nn'

Cuts at:      0    3606    4293 
   Size:       3606     687
 
BsrFI r'CCGG_y

Cuts at:      0     191    3583    4293 
   Size:        191    3392     710

  Fragments arranged by size: 

                3392     710     191
 
BssSI C'ACGA_G

Cuts at:      0    1837    4293 
   Size:       1837    2456
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     253    4293 
   Size:        253    4040
 
BstEII G'GTnAC_C

Cuts at:      0     369    4293 
   Size:        369    3924
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     481    4293 
   Size:        481    3812
 
BstYI r'GATC_y

Cuts at:      0     944    1283    1385    4293 
   Size:        944     339     102    2908

  Fragments arranged by size: 

                2908     944     339     102
 
BtgI C'CryG_G

Cuts at:      0     170    1288    2564    4293 
   Size:        170    1118    1276    1729

  Fragments arranged by size: 

                1729    1276    1118     170
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2096    2216    3962    4293 
   Size:       2096     120    1746     331

  Fragments arranged by size: 

                2096    1746     331     120
 
BtsI GCAGTG_nn'

Cuts at:      0     395    1931    2424    2616    2946    3066    3486    4020 
   Size:        395    1536     493     192     330     120     420     534

Cuts at:   4020    4140    4257    4293 
   Size:        120     117      36

  Fragments arranged by size: 

                1536     534     493     420     395     330     192     120
                 120     117      36
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2829    2864    4293 
   Size:       2829      35    1429

  Fragments arranged by size: 

                2829    1429      35
 
DraI TTT'AAA

Cuts at:      0     320    4293 
   Size:        320    3973
 
EaeI y'GGCC_r

Cuts at:      0     167     471    1322    4293 
   Size:        167     304     851    2971

  Fragments arranged by size: 

                2971     851     304     167
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     421    2066    2829    4293 
   Size:        107     314    1645     763    1464

  Fragments arranged by size: 

                1645    1464     763     314     107
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1296    3827    4293 
   Size:       1296    2531     466

  Fragments arranged by size: 

                2531    1296     466
 
EcoICRI GAG'CTC

Cuts at:      0    3454    4293 
   Size:       3454     839
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     245     374     517    1216    1498    1789    1991 
   Size:         71     174     129     143     699     282     291     202

Cuts at:   1991    2118    2151    2181    2223    2235    2289    2337    2343 
   Size:        127      33      30      42      12      54      48       6

Cuts at:   2343    2619    2697    3219    3249    3669    3789    4053    4083 
   Size:        276      78     522      30     420     120     264      30

Cuts at:   4083    4140    4293 
   Size:         57     153

  Fragments arranged by size: 

                 699     522     420     291     282     276     264     202
                 174     153     143     129     127     120      78      71
                  57      54      48      42      33      30      30      30
                  12       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45      46     219     513     924     954    1088    1161 
   Size:         45       1     173     294     411      30     134      73

Cuts at:   1161    1211    1782    2027    4293 
   Size:         50     571     245    2266

  Fragments arranged by size: 

                2266     571     411     294     245     173     134      73
                  50      45      30       1
 
EcoRV GAT'ATC

Cuts at:      0    2052    4293 
   Size:       2052    2241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     522    4293 
   Size:        522    3771
 
FspI TGC'GCA

Cuts at:      0    1678    4293 
   Size:       1678    2615
 
HaeII r_GCGC'y

Cuts at:      0    3740    4293 
   Size:       3740     553
 
HincII GTy'rAC

Cuts at:      0     189    2163    4293 
   Size:        189    1974    2130

  Fragments arranged by size: 

                2130    1974     189
 
HindIII A'AGCT_T

Cuts at:      0     611    4293 
   Size:        611    3682
 
KpnI G_GTAC'C

Cuts at:      0    3734    4293 
   Size:       3734     559
 
MfeI C'AATT_G

Cuts at:      0     554     788    4293 
   Size:        554     234    3505

  Fragments arranged by size: 

                3505     554     234
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    4046    4293 
   Size:       4046     247
 
MscI TGG'CCA

Cuts at:      0     169     473    1324    4293 
   Size:        169     304     851    2969

  Fragments arranged by size: 

                2969     851     304     169
 
MslI CAynn'nnrTG

Cuts at:      0     479    1247    1630    4293 
   Size:        479     768     383    2663

  Fragments arranged by size: 

                2663     768     479     383
 
MspA1I CmG'CkG

Cuts at:      0     271     495    1951    3346    4103    4160    4293 
   Size:        271     224    1456    1395     757      57     133

  Fragments arranged by size: 

                1456    1395     757     271     224     133      57
 
NcoI C'CATG_G

Cuts at:      0     170    1288    4293 
   Size:        170    1118    3005

  Fragments arranged by size: 

                3005    1118     170
 
NsiI A_TGCA'T

Cuts at:      0     137    4293 
   Size:        137    4156
 
NspI r_CATG'y

Cuts at:      0    1410    1874    2448    4293 
   Size:       1410     464     574    1845

  Fragments arranged by size: 

                1845    1410     574     464
 
PasI CC'CwG_GG

Cuts at:      0    1696    4293 
   Size:       1696    2597
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     990    1164    1901    4117    4204    4293 
   Size:        990     174     737    2216      87      89

  Fragments arranged by size: 

                2216     990     737     174      89      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     513    1211    1782    4293 
   Size:        513     698     571    2511

  Fragments arranged by size: 

                2511     698     571     513
 
PspOMI G'GGCC_C

Cuts at:      0      45     924    1088    2026    4293 
   Size:         45     879     164     938    2267

  Fragments arranged by size: 

                2267     938     879     164      45
 
PstI C_TGCA'G

Cuts at:      0    1563    1950    4135    4293 
   Size:       1563     387    2185     158

  Fragments arranged by size: 

                2185    1563     387     158
 
PvuII CAG'CTG

Cuts at:      0     271     495    1951    4293 
   Size:        271     224    1456    2342

  Fragments arranged by size: 

                2342    1456     271     224
 
SacI G_AGCT'C

Cuts at:      0    3456    4293 
   Size:       3456     837
 
SanDI GG'GwC_CC

Cuts at:      0    1782    4293 
   Size:       1782    2511
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2829    4293 
   Size:       2829    1464
 
ScaI AGT'ACT

Cuts at:      0    2958    4293 
   Size:       2958    1335
 
SfcI C'TryA_G

Cuts at:      0    1559    1946    4131    4293 
   Size:       1559     387    2185     162

  Fragments arranged by size: 

                2185    1559     387     162
 
SmaI CCC'GGG

Cuts at:      0      62    1216    4293 
   Size:         62    1154    3077

  Fragments arranged by size: 

                3077    1154      62
 
SmlI C'TyrA_G

Cuts at:      0    1132    1861    4293 
   Size:       1132     729    2432

  Fragments arranged by size: 

                2432    1132     729
 
SpeI A'CTAG_T

Cuts at:      0    2407    3853    4293 
   Size:       2407    1446     440

  Fragments arranged by size: 

                2407    1446     440
 
SphI G_CATG'C

Cuts at:      0    1874    2448    4293 
   Size:       1874     574    1845

  Fragments arranged by size: 

                1874    1845     574
 
SspI AAT'ATT

Cuts at:      0     543    1441    4293 
   Size:        543     898    2852

  Fragments arranged by size: 

                2852     898     543
 
StuI AGG'CCT

Cuts at:      0     868    1302    2095    3966    4293 
   Size:        868     434     793    1871     327

  Fragments arranged by size: 

                1871     868     793     434     327
 
StyI C'CwwG_G

Cuts at:      0     155     170     440     450     617     702     899    1083 
   Size:        155      15     270      10     167      85     197     184

Cuts at:   1083    1288    1580    1895    3666    4293 
   Size:        205     292     315    1771     627

  Fragments arranged by size: 

                1771     627     315     292     270     205     197     184
                 167     155      85      15      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     700     715    1605    2247    2301    2631    4182    4242 
   Size:        700      15     890     642      54     330    1551      60

Cuts at:   4242    4293 
   Size:         51

  Fragments arranged by size: 

                1551     890     700     642     330      60      54      51
                  15
 
TatI w'GTAC_w

Cuts at:      0    1772    2583    2956    4293 
   Size:       1772     811     373    1337

  Fragments arranged by size: 

                1772    1337     811     373
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46    4293 
   Size:         14      32    4247

  Fragments arranged by size: 

                4247      32      14
 
Tth111I GACn'n_nGTC

Cuts at:      0     121    4293 
   Size:        121    4172
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     466    2002    4293 
   Size:        162     304    1536    2291

  Fragments arranged by size: 

                2291    1536     304     162
 
XmaI C'CCGG_G

Cuts at:      0      60    1214    4293 
   Size:         60    1154    3079

  Fragments arranged by size: 

                3079    1154      60
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AgeI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI     FspI    HaeII   HincII  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI
    PflMI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI
     ScaI     SfcI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AhdI     AleI     AlfI
     AloI     AscI     AseI    AsiSI     BaeI     BbeI     BcgI     BclI
    BmgBI     BmtI    BsaAI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI
   DraIII     DrdI     EagI    EcoNI    EcoRI     FseI    FspAI    Hin4I
     HpaI     KasI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SrfI
     SwaI     XbaI     XhoI     XmnI     ZraI
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