Restriction map for pF1KA1042
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AflII       AgeI        AhdI        ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BlnI        BsaAI       BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DraIII      Eam1105I    EcoRI       
EcoRV       EcoT22I     FbaI        HpaI        KasI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfoI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       2667  GGTACC               G^GTACC
AcyI         1455  GACGCC               G[GA]^CG[CT]C
AflIII       2504  ACGTGT               A^C[GA][CT]GT
Alw44I       2400  GTGCAC               G^TGCAC
Aor51HI       538  AGCGCT               AGC^GCT
ApaI         2626  GGGCCC               GGGCC^C
ApaLI        2400  GTGCAC               G^TGCAC
Asp718I      2667  GGTACC               G^GTACC
AspI         2533  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglI         2628  GCCCCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1246  AGATCT               A^GATCT
BsaHI        1455  GACGCC               G[GA]^CG[CT]C
BsgI         2302  CTGCAC               GTGCAG(16/14)
BsiEI        2286  CGGCCG               CG[GA][CT]^CG
EagI         2286  CGGCCG               C^GGCCG
EclXI        2286  CGGCCG               C^GGCCG
Eco47III      538  AGCGCT               AGC^GCT
Eco52I       2286  CGGCCG               C^GGCCG
FseI         2283  GGCCGGCC             GGCCGG^CC
Hin1I        1455  GACGCC               G[GA]^CG[CT]C
HincII       1849  GTTGAC               GT[CT]^[GA]AC
HindII       1849  GTTGAC               GT[CT]^[GA]AC
HindIII       896  AAGCTT               A^AGCTT
KpnI         2667  GGTACC               GGTAC^C
NaeI         2284  GCCGGC               GCC^GGC
NgoMIV       2284  GCCGGC               G^CCGGC
PflFI        2533  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2610  GGGACCC              [GA]G^G[AT]CC[CT]
SacI         2044  GAGCTC               GAGCT^C
SexAI        1229  ACCAGGT              A^CC[AT]GGT
SfiI         2627  GGCCCCCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2120  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI         2631  CCCGGG               CCC^GGG
SmlI          355  CTTGAG               C^T[CT][GA]AG
SphI         2780  GCATGC               GCATG^C
StuI         2661  AGGCCT               AGG^CCT
Tth111I      2533  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2631  CCCGGG               C^CCGGG
XmaI         2631  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AocI         2454  CCTGAGG              CC^T[ACGT]AGG
             2671  CCTGAGG             
BanI         1462  GGCACC               G^G[CT][GA]CC
             2667  GGTACC              
BbsI          702  GAAGAC               GAAGAC(2/6)
              816  GAAGAC              
BlpI           37  GCTCAGC              GC^T[ACGT]AGC
             2830  GCTAAGC             
Bpu1102I       37  GCTCAGC              GC^T[ACGT]AGC
             2830  GCTAAGC             
BpuAI         702  GAAGAC               GAAGAC(2/6)
              816  GAAGAC              
BsaI          709  GAGACC               GGTCTC(1/5)
             1954  GAGACC              
BstEII       1821  GGTCACC              G^GT[ACGT]ACC
             1900  GGTGACC             
BstPI        1821  GGTCACC              G^GT[ACGT]ACC
             1900  GGTGACC             
BstYI        1246  AGATCT               [GA]^GATC[CT]
             2773  GGATCT              
Bsu36I       2454  CCTGAGG              CC^T[ACGT]AGG
             2671  CCTGAGG             
CelII          37  GCTCAGC              GC^T[ACGT]AGC
             2830  GCTAAGC             
DrdI         2499  GACCGACGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2503  GACGTGTCCGTC        
Eco81I       2454  CCTGAGG              CC^T[ACGT]AGG
             2671  CCTGAGG             
EcoO65I      1821  GGTCACC              G^GT[ACGT]ACC
             1900  GGTGACC             
MflI         1246  AGATCT               [GA]^GATC[CT]
             2773  GGATCT              
NcoI         1109  CCATGG               C^CATGG
             2765  CCATGG              
PstI         1156  CTGCAG               CTGCA^G
             2722  CTGCAG              
SfcI         1156  CTGCAG               C^T[GA][CT]AG
             2722  CTGCAG              
XcmI         1109  CCATGGATTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1779  CCAACCTCACCTTGG     
XhoII        1246  AGATCT               [GA]^GATC[CT]
             2773  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       AviII       
BalI        BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseAI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      
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