Restriction map for pF1KA1042
 (Linear) (Six-base) MAPSORT of: KIAA1042.seq  Check: 2381  from: 1  to: 2859



KIAA1042.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     447     866    2859 
   Size:        447     419    1993

  Fragments arranged by size: 

                1993     447     419
 
Acc65I G'GTAC_C

Cuts at:      0    2667    2859 
   Size:       2667     192
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     721    1060    1674    2021    2859 
   Size:        508     213     339     614     347     838

  Fragments arranged by size: 

                 838     614     508     347     339     213
 
AfeI AGC'GCT

Cuts at:      0     540    2859 
   Size:        540    2319
 
AflIII A'CryG_T

Cuts at:      0    2504    2859 
   Size:       2504     355
 
AleI CACnn'nnGTG

Cuts at:      0    2122    2767    2859 
   Size:       2122     645      92

  Fragments arranged by size: 

                2122     645      92
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     915    1030    1171    2859 
   Size:        915     115     141    1688

  Fragments arranged by size: 

                1688     915     141     115
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1525    1557    2859 
   Size:       1525      32    1302

  Fragments arranged by size: 

                1525    1302      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45     741     933    1578    2859 
   Size:         45     696     192     645    1281

  Fragments arranged by size: 

                1281     696     645     192      45
 
ApaI G_GGCC'C

Cuts at:      0    2630    2859 
   Size:       2630     229
 
ApaLI G'TGCA_C

Cuts at:      0    2400    2859 
   Size:       2400     459
 
AvaI C'yCGr_G

Cuts at:      0     559    2051    2631    2811    2859 
   Size:        559    1492     580     180      48

  Fragments arranged by size: 

                1492     580     559     180      48
 
BanI G'GyrC_C

Cuts at:      0    1462    2667    2859 
   Size:       1462    1205     192

  Fragments arranged by size: 

                1462    1205     192
 
BanII G_rGCy'C

Cuts at:      0      39     560    2048    2219    2296    2410    2583    2630 
   Size:         39     521    1488     171      77     114     173      47

Cuts at:   2630    2859 
   Size:        229

  Fragments arranged by size: 

                1488     521     229     173     171     114      77      47
                  39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     709     823    2859 
   Size:        709     114    2036

  Fragments arranged by size: 

                2036     709     114
 
BbvCI CC'TCA_GC

Cuts at:      0     766    1495    1959    2859 
   Size:        766     729     464     900

  Fragments arranged by size: 

                 900     766     729     464
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2634    2859 
   Size:       2634     225
 
BglII A'GATC_T

Cuts at:      0    1246    2859 
   Size:       1246    1613
 
BlpI GC'TnA_GC

Cuts at:      0      38    2831    2859 
   Size:         38    2793      28

  Fragments arranged by size: 

                2793      38      28
 
Bme1580I G_kGCm'C

Cuts at:      0    1465    1645    2404    2630    2859 
   Size:       1465     180     759     226     229

  Fragments arranged by size: 

                1465     759     229     226     180
 
BmgBI CAC'GTC

Cuts at:      0    1076    2505    2859 
   Size:       1076    1429     354

  Fragments arranged by size: 

                1429    1076     354
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     618    2859 
   Size:        618    2241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     212     469    1006    1163    1504    1555    1705 
   Size:        160      52     257     537     157     341      51     150

Cuts at:   1705    1922    2069    2859 
   Size:        217     147     790

  Fragments arranged by size: 

                 790     537     341     257     217     160     157     150
                 147      52      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     766     944    1495    1959    2859 
   Size:        766     178     551     464     900

  Fragments arranged by size: 

                 900     766     551     464     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    2859 
   Size:        376    2483
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     703    1948    2859 
   Size:        703    1245     911

  Fragments arranged by size: 

                1245     911     703
 
BsaHI Gr'CG_yC

Cuts at:      0    1456    2859 
   Size:       1456    1403
 
BsaWI w'CCGG_w

Cuts at:      0     114    1049    1835    2859 
   Size:        114     935     786    1024

  Fragments arranged by size: 

                1024     935     786     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     466     496     979    1009    1144    1174    1245    1275 
   Size:        466      30     483      30     135      30      71      30

Cuts at:   1275    1525    1555    1920    1950    2394    2424    2859 
   Size:        250      30     365      30     444      30     435

  Fragments arranged by size: 

                 483     466     444     435     365     250     135      71
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     373     466     490     562     594     679     739 
   Size:        157     216      93      24      72      32      85      60

Cuts at:    739     859    1048    1551    1564    1567    2608    2617    2859 
   Size:        120     189     503      13       3    1041       9     242

  Fragments arranged by size: 

                1041     503     242     216     189     157     120      93
                  85      72      60      32      24      13       9       3
            
 
BseYI C'CCAG_C

Cuts at:      0     953    1095    2273    2859 
   Size:        953     142    1178     586

  Fragments arranged by size: 

                1178     953     586     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2287    2859 
   Size:       2287     572
 
BsiEI CG_ry'CG

Cuts at:      0    2289    2859 
   Size:       2289     570
 
BsiHKAI G_wGCw'C

Cuts at:      0    2048    2206    2404    2859 
   Size:       2048     158     198     455

  Fragments arranged by size: 

                2048     455     198     158
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     262    1082    1480    2517    2859 
   Size:        262     820     398    1037     342

  Fragments arranged by size: 

                1037     820     398     342     262
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     560    1465    1645    2048    2206    2219    2296 
   Size:         39     521     905     180     403     158      13      77

Cuts at:   2296    2404    2410    2583    2630    2859 
   Size:        108       6     173      47     229

  Fragments arranged by size: 

                 905     521     403     229     180     173     158     108
                  77      47      39      13       6
 
BspEI T'CCGG_A

Cuts at:      0     114    1049    1835    2859 
   Size:        114     935     786    1024

  Fragments arranged by size: 

                1024     935     786     114
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     447     866    1715    2859 
   Size:        447     419     849    1144

  Fragments arranged by size: 

                1144     849     447     419
 
BsrBI CCG'CTC

Cuts at:      0     369     388    1437    1700    2238    2859 
   Size:        369      19    1049     263     538     621

  Fragments arranged by size: 

                1049     621     538     369     263      19
 
BsrFI r'CCGG_y

Cuts at:      0    1669    2121    2284    2859 
   Size:       1669     452     163     575

  Fragments arranged by size: 

                1669     575     452     163
 
BssSI C'ACGA_G

Cuts at:      0    2218    2403    2527    2859 
   Size:       2218     185     124     332

  Fragments arranged by size: 

                2218     332     185     124
 
BstEII G'GTnAC_C

Cuts at:      0    1821    1900    2859 
   Size:       1821      79     959

  Fragments arranged by size: 

                1821     959      79
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2124    2664    2769    2859 
   Size:       2124     540     105      90

  Fragments arranged by size: 

                2124     540     105      90
 
BstYI r'GATC_y

Cuts at:      0    1246    2773    2859 
   Size:       1246    1527      86

  Fragments arranged by size: 

                1527    1246      86
 
Bsu36I CC'TnA_GG

Cuts at:      0    2455    2672    2859 
   Size:       2455     217     187

  Fragments arranged by size: 

                2455     217     187
 
BtgI C'CryG_G

Cuts at:      0      60    1109    2143    2765    2859 
   Size:         60    1049    1034     622      94

  Fragments arranged by size: 

                1049    1034     622      94      60
 
BtsI GCAGTG_nn'

Cuts at:      0     911    2859 
   Size:        911    1948
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2505    2509    2859 
   Size:       2505       4     350

  Fragments arranged by size: 

                2505     350       4
 
EaeI y'GGCC_r

Cuts at:      0     306     383     396     809    1295    2126    2286    2555 
   Size:        306      77      13     413     486     831     160     269

Cuts at:   2555    2859 
   Size:        304

  Fragments arranged by size: 

                 831     486     413     306     304     269     160      77
                  13
 
EagI C'GGCC_G

Cuts at:      0    2286    2859 
   Size:       2286     573
 
EarI CTCTTCn'nnn_

Cuts at:      0     260     580     641     656    1160    2859 
   Size:        260     320      61      15     504    1699

  Fragments arranged by size: 

                1699     504     320     260      61      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1597    2333    2760    2859 
   Size:       1597     736     427      99

  Fragments arranged by size: 

                1597     736     427      99
 
EcoICRI GAG'CTC

Cuts at:      0    2046    2859 
   Size:       2046     813
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     212     469     508     721    1006    1060    1163 
   Size:        160      52     257      39     213     285      54     103

Cuts at:   1163    1504    1555    1674    1705    1922    2021    2069    2859 
   Size:        341      51     119      31     217      99      48     790

  Fragments arranged by size: 

                 790     341     285     257     217     213     160     119
                 103      99      54      52      51      48      39      31
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      47     139     661    2859 
   Size:         47      92     522    2198

  Fragments arranged by size: 

                2198     522      92      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2611    2627    2676    2859 
   Size:       2611      16      49     183

  Fragments arranged by size: 

                2611     183      49      16
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1221    2859 
   Size:       1221    1638
 
FseI GG_CCGG'CC

Cuts at:      0    2288    2859 
   Size:       2288     571
 
FspI TGC'GCA

Cuts at:      0     903    1147    1600    2859 
   Size:        903     244     453    1259

  Fragments arranged by size: 

                1259     903     453     244
 
HaeII r_GCGC'y

Cuts at:      0     542    1086    1495    2859 
   Size:        542     544     409    1364

  Fragments arranged by size: 

                1364     544     542     409
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     466     498     547     579    1159    1191    1245    1277 
   Size:        466      32      49      32     580      32      54      32

Cuts at:   1277    2570    2602    2603    2635    2778    2810    2859 
   Size:       1293      32       1      32     143      32      49

  Fragments arranged by size: 

                1293     580     466     143      54      49      49      32
                  32      32      32      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0    1851    2859 
   Size:       1851    1008
 
HindIII A'AGCT_T

Cuts at:      0     896    2859 
   Size:        896    1963
 
KpnI G_GTAC'C

Cuts at:      0    2671    2859 
   Size:       2671     188
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1142    1307    2540    2859 
   Size:       1142     165    1233     319

  Fragments arranged by size: 

                1233    1142     319     165
 
MscI TGG'CCA

Cuts at:      0     308     811    2128    2557    2859 
   Size:        308     503    1317     429     302

  Fragments arranged by size: 

                1317     503     429     308     302
 
MslI CAynn'nnrTG

Cuts at:      0    1012    2122    2767    2785    2821    2859 
   Size:       1012    1110     645      18      36      38

  Fragments arranged by size: 

                1110    1012     645      38      36      18
 
MspA1I CmG'CkG

Cuts at:      0     150     699     741     957    1319    1604    1737    2859 
   Size:        150     549      42     216     362     285     133    1122

  Fragments arranged by size: 

                1122     549     362     285     216     150     133      42
            
 
NaeI GCC'GGC

Cuts at:      0    2286    2859 
   Size:       2286     573
 
NcoI C'CATG_G

Cuts at:      0    1109    2765    2859 
   Size:       1109    1656      94

  Fragments arranged by size: 

                1656    1109      94
 
NgoMIV G'CCGG_C

Cuts at:      0    2284    2859 
   Size:       2284     575
 
NspI r_CATG'y

Cuts at:      0    1686    1986    2784    2859 
   Size:       1686     300     798      75

  Fragments arranged by size: 

                1686     798     300      75
 
PasI CC'CwG_GG

Cuts at:      0    1638    2211    2859 
   Size:       1638     573     648

  Fragments arranged by size: 

                1638     648     573
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     933    1095    1482    1578    2092    2859 
   Size:        933     162     387      96     514     767

  Fragments arranged by size: 

                 933     767     514     387     162      96
 
PfoI T'CCnGG_A

Cuts at:      0    1571    1799    2859 
   Size:       1571     228    1060

  Fragments arranged by size: 

                1571    1060     228
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1513    1525    1545    1557    2859 
   Size:       1513      12      20      12    1302

  Fragments arranged by size: 

                1513    1302      20      12      12
 
PpuMI rG'GwC_Cy

Cuts at:      0    2611    2859 
   Size:       2611     248
 
PsiI TTA'TAA

Cuts at:      0     161    2859 
   Size:        161    2698
 
PspOMI G'GGCC_C

Cuts at:      0    2626    2859 
   Size:       2626     233
 
PstI C_TGCA'G

Cuts at:      0    1160    2726    2859 
   Size:       1160    1566     133

  Fragments arranged by size: 

                1566    1160     133
 
PvuII CAG'CTG

Cuts at:      0     150     699     741    1319    2859 
   Size:        150     549      42     578    1540

  Fragments arranged by size: 

                1540     578     549     150      42
 
SacI G_AGCT'C

Cuts at:      0    2048    2859 
   Size:       2048     811
 
SanDI GG'GwC_CC

Cuts at:      0    2611    2859 
   Size:       2611     248
 
SexAI A'CCwGG_T

Cuts at:      0    1229    2859 
   Size:       1229    1630
 
SfcI C'TryA_G

Cuts at:      0    1156    2722    2859 
   Size:       1156    1566     137

  Fragments arranged by size: 

                1566    1156     137
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2634    2859 
   Size:       2634     225
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2121    2859 
   Size:       2121     738
 
SmaI CCC'GGG

Cuts at:      0    2633    2859 
   Size:       2633     226
 
SmlI C'TyrA_G

Cuts at:      0     355    2859 
   Size:        355    2504
 
SphI G_CATG'C

Cuts at:      0    2784    2859 
   Size:       2784      75
 
StuI AGG'CCT

Cuts at:      0    2663    2859 
   Size:       2663     196
 
StyI C'CwwG_G

Cuts at:      0    1109    1118    1788    1826    2765    2859 
   Size:       1109       9     670      38     939      94

  Fragments arranged by size: 

                1109     939     670      94      38       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1632    1964    2382    2515    2815    2859 
   Size:       1632     332     418     133     300      44

  Fragments arranged by size: 

                1632     418     332     300     133      44
 
TatI w'GTAC_w

Cuts at:      0     675    1865    2859 
   Size:        675    1190     994

  Fragments arranged by size: 

                1190     994     675
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      96     128    2098    2130    2394    2426    2859 
   Size:         96      32    1970      32     264      32     433

  Fragments arranged by size: 

                1970     433     264      96      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2536    2859 
   Size:       2536     323
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1116    1786    2859 
   Size:       1116     670    1073

  Fragments arranged by size: 

                1116    1073     670
 
XmaI C'CCGG_G

Cuts at:      0    2631    2859 
   Size:       2631     228
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SacI    SanDI    SexAI     SfcI     SfiI    SgrAI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI     BmrI     BmtI    BsaAI    BsaBI    BsiWI     BsmI
    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV    FspAI     HpaI
     KasI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI    PshAI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI     SfoI
     SgfI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI
     XmnI     ZraI
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