Restriction map for pF1KA1033
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BglI        BlnI        BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       FauI        FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KspI        MluI        
MroI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        Sse8387I    SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

Acc65I       1038  GGTACC               G^GTACC
AflII          82  CTTAAG               C^TTAAG
AhdI         3335  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2707  CCTGAGG              CC^T[ACGT]AGG
AseI         2452  ATTAAT               AT^TAAT
AsnI         2452  ATTAAT               AT^TAAT
Asp700I      1726  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1038  GGTACC               G^GTACC
AspEI        3335  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           12  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1553  TGGCCA               TGG^CCA
BanI         1038  GGTACC               G^G[CT][GA]CC
BanII        1227  GAGCTC               G[GA]GC[CT]^C
BbvCI         853  GCTGAGG              CCTCAGC(-5/-2)
BcnI           21  CCCGG                CC^[GC]GG
BfrI           82  CTTAAG               C^TTAAG
BlpI         3262  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     3262  GCTAAGC              GC^T[ACGT]AGC
BsaAI        2182  TACGTA               [CT]AC^GT[GA]
BsmI         3078  GCATTC               GAATGC(1/-1)
BspMI        1923  ACCTGC               ACCTGC(4/8)
Bst1107I      111  GTATAC               GTA^TAC
BstZ17I       111  GTATAC               GTA^TAC
Bsu36I       2707  CCTGAGG              CC^T[ACGT]AGG
CelII        3262  GCTAAGC              GC^T[ACGT]AGC
CfoI         1911  GCGC                 GCG^C
DraII        2333  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI         3152  CCATGG               C^C[GA][CT]GG
EaeI         1553  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3335  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1535  CTCTTC               CTCTTC(1/4)
Eco81I       2707  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     2333  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         704  GAATTC               G^AATTC
EcoT14I      3152  CCATGG               C^C[AT][AT]GG
HapII          22  CCGG                 C^CGG
HhaI         1911  GCGC                 GCG^C
HinP1I       1911  GCGC                 G^CGC
HincII         43  GTTGAC               GT[CT]^[GA]AC
HindII         43  GTTGAC               GT[CT]^[GA]AC
HpaII          22  CCGG                 C^CGG
KpnI         1038  GGTACC               GGTAC^C
Ksp632I      1535  CTCTTC               CTCTTC(1/4)
MfeI          155  CAATTG               C^AATTG
MluNI        1553  TGGCCA               TGG^CCA
MscI         1553  TGGCCA               TGG^CCA
MspA1I       1109  CAGCTG               C[AC]G^C[GT]G
MspI           22  CCGG                 C^CGG
MunI          155  CAATTG               C^AATTG
NciI           21  CCCGG                CC^[GC]GG
NcoI         3152  CCATGG               C^CATGG
NdeI         1462  CATATG               CA^TATG
PflFI          12  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         486  CCACCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2452  ATTAAT               AT^TAAT
PvuII        1109  CAGCTG               CAG^CTG
SacI         1227  GAGCTC               GAGCT^C
SapI         1534  GCTCTTC              GCTCTTC(1/4)
ScaI          844  AGTACT               AGT^ACT
SnaBI        2182  TACGTA               TAC^GTA
SpeI         2865  ACTAGT               A^CTAGT
SphI         1935  GCATGC               GCATG^C
StuI          785  AGGCCT               AGG^CCT
StyI         3152  CCATGG               C^C[AT][AT]GG
Tsp45I       1802  GTGAC                ^GT[GC]AC
Tth111I        12  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        486  CCACCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2315  CCAGTCAGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1726  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          111  GTATAC               GT^[AC][GT]AC
             2983  GTAGAC              
AccII          40  CGCG                 CG^CG
             1912  CGCG                
AflIII       1382  ACATGT               A^C[GA][CT]GT
             1895  ACATGT              
AlwNI         433  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1106  CAGCAGCTG           
AvaII        1645  GGACC                G^G[AT]CC
             2631  GGACC               
BciVI        1161  GGATAC               GTATCC(6/5)
             2649  GTATCC              
BpmI         2996  CTCCAG               CTGGAG(16/14)
              545  CTGGAG              
BsaI         3476  GAGACC               GGTCTC(1/5)
              343  GGTCTC              
BseRI         106  GAGGAG               GAGGAG(10/8)
              821  GAGGAG              
BsgI          284  CTGCAC               GTGCAG(16/14)
             1428  GTGCAG              
BspHI        1748  TCATGA               T^CATGA
             2235  TCATGA              
BspLU11I     1382  ACATGT               A^CATGT
             1895  ACATGT              
BsrDI        2813  GCAATG               GCAATG(2/0)
             3470  GCAATG              
BstUI          40  CGCG                 CG^CG
             1912  CGCG                
BstXI         377  CCAGCATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3152  CCATGGGTGTGG        
DraIII       1685  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1796  CACAATGTG           
EcoT22I      1337  ATGCAT               ATGCA^T
             1933  ATGCAT              
HgaI          139  GACGC                GACGC(5/10)
             1596  GCGTC               
HindIII       199  AAGCTT               A^AGCTT
             3175  AAGCTT              
MvnI           40  CGCG                 CG^CG
             1912  CGCG                
NlaIV        1038  GGTACC               GG[ACGT]^[ACGT]CC
             3215  GGTTCC              
NsiI         1337  ATGCAT               ATGCA^T
             1933  ATGCAT              
RcaI         1748  TCATGA               T^CATGA
             2235  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BbsI        BbvI        BclI        
BfaI        BglII       BmyI        BpuAI       BsaBI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BsrGI       BsrI        
BssKI       BstBI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EcoRII      FbaI        Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NspI        NspV        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfuI        SmlI        SspBI       SspI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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