Restriction map for pF1KA1033
 (Linear) (Six-base) MAPSORT of: KIAA1033.seq  Check: 8929  from: 1  to: 3519



KIAA1033.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0     112    2984    3519 
   Size:        112    2872     535

  Fragments arranged by size: 

                2872     535     112
 
Acc65I G'GTAC_C

Cuts at:      0    1038    3519 
   Size:       1038    2481
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1310    3262    3384    3443    3519 
   Size:        766     544    1952     122      59      76

  Fragments arranged by size: 

                1952     766     544     122      76      59
 
AflII C'TTAA_G

Cuts at:      0      82    3519 
   Size:         82    3437
 
AflIII A'CryG_T

Cuts at:      0    1382    1895    3519 
   Size:       1382     513    1624

  Fragments arranged by size: 

                1624    1382     513
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3340    3519 
   Size:       3340     179
 
AlwNI CAG_nnn'CTG

Cuts at:      0     438    1111    3519 
   Size:        438     673    2408

  Fragments arranged by size: 

                2408     673     438
 
ApoI r'AATT_y

Cuts at:      0      62      97     331     401     697     704     859     950 
   Size:         62      35     234      70     296       7     155      91

Cuts at:    950    1282    1997    2671    2853    2946    3001    3033    3355 
   Size:        332     715     674     182      93      55      32     322

Cuts at:   3355    3392    3519 
   Size:         37     127

  Fragments arranged by size: 

                 715     674     332     322     296     234     182     155
                 127      93      91      70      62      55      37      35
                  32       7
 
AseI AT'TA_AT

Cuts at:      0    2453    3519 
   Size:       2453    1066
 
BanI G'GyrC_C

Cuts at:      0    1038    3519 
   Size:       1038    2481
 
BanII G_rGCy'C

Cuts at:      0    1231    3519 
   Size:       1231    2288
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1129    1827    2791    3519 
   Size:       1129     698     964     728

  Fragments arranged by size: 

                1129     964     728     698
 
BbvCI CC'TCA_GC

Cuts at:      0     854    3519 
   Size:        854    2665
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1155    2660    3519 
   Size:       1155    1505     859

  Fragments arranged by size: 

                1505    1155     859
 
BclI T'GATC_A

Cuts at:      0    2640    2727    2934    3519 
   Size:       2640      87     207     585

  Fragments arranged by size: 

                2640     585     207      87
 
BfrBI ATG'CAT

Cuts at:      0    1339    1935    3519 
   Size:       1339     596    1584

  Fragments arranged by size: 

                1584    1339     596
 
BglII A'GATC_T

Cuts at:      0    1293    2046    2788    3519 
   Size:       1293     753     742     731

  Fragments arranged by size: 

                1293     753     742     731
 
BlpI GC'TnA_GC

Cuts at:      0    3263    3519 
   Size:       3263     256
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      36    2250    2309    3519 
   Size:         36    2214      59    1210

  Fragments arranged by size: 

                2214    1210      59      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     132    3519 
   Size:        132    3387
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566    2981    3519 
   Size:        566    2415     538

  Fragments arranged by size: 

                2415     566     538
 
Bpu10I CC'TnA_GC

Cuts at:      0     854    3519 
   Size:        854    2665
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124    1432    3519 
   Size:        124    1308    2087

  Fragments arranged by size: 

                2087    1308     124
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     349    3470    3519 
   Size:        349    3121      49

  Fragments arranged by size: 

                3121     349      49
 
BsaAI yAC'GTr

Cuts at:      0    2184    3519 
   Size:       2184    1335
 
BsaBI GATnn'nnATC

Cuts at:      0     176    3188    3344    3519 
   Size:        176    3012     156     175

  Fragments arranged by size: 

                3012     176     175     156
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      31     815     845    3519 
   Size:          1      30     784      30    2674

  Fragments arranged by size: 

                2674     784      30      30       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     836    3519 
   Size:        121     715    2683

  Fragments arranged by size: 

                2683     715     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269    1449    3519 
   Size:        269    1180    2070

  Fragments arranged by size: 

                2070    1180     269
 
BsiHKAI G_wGCw'C

Cuts at:      0     575    1231    1610    3519 
   Size:        575     656     379    1909

  Fragments arranged by size: 

                1909     656     575     379
 
BsmI GAATG_Cn'

Cuts at:      0    3078    3519 
   Size:       3078     441
 
Bsp1286I G_dGCh'C

Cuts at:      0     575    1231    1610    3519 
   Size:        575     656     379    1909

  Fragments arranged by size: 

                1909     656     575     379
 
BspHI T'CATG_A

Cuts at:      0    1748    2235    3519 
   Size:       1748     487    1284

  Fragments arranged by size: 

                1748    1284     487
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1932    3519 
   Size:       1932    1587
 
BsrDI GCAATG_nn'

Cuts at:      0    2820    3477    3519 
   Size:       2820     657      42

  Fragments arranged by size: 

                2820     657      42
 
BsrGI T'GTAC_A

Cuts at:      0     656    1203    3276    3519 
   Size:        656     547    2073     243

  Fragments arranged by size: 

                2073     656     547     243
 
BstBI TT'CG_AA

Cuts at:      0     987    2481    3006    3519 
   Size:        987    1494     525     513

  Fragments arranged by size: 

                1494     987     525     513
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384    3159    3519 
   Size:        384    2775     360

  Fragments arranged by size: 

                2775     384     360
 
BstYI r'GATC_y

Cuts at:      0    1293    1761    2046    2788    3519 
   Size:       1293     468     285     742     731

  Fragments arranged by size: 

                1293     742     731     468     285
 
BstZ17I GTA'TAC

Cuts at:      0     113    3519 
   Size:        113    3406
 
Bsu36I CC'TnA_GG

Cuts at:      0    2708    3519 
   Size:       2708     811
 
BtgI C'CryG_G

Cuts at:      0    3152    3519 
   Size:       3152     367
 
BtsI GCAGTG_nn'

Cuts at:      0    3390    3519 
   Size:       3390     129
 
DraI TTT'AAA

Cuts at:      0    1716    2003    2075    3519 
   Size:       1716     287      72    1444

  Fragments arranged by size: 

                1716    1444     287      72
 
DraIII CAC_nnn'GTG

Cuts at:      0    1690    1801    3519 
   Size:       1690     111    1718

  Fragments arranged by size: 

                1718    1690     111
 
EaeI y'GGCC_r

Cuts at:      0    1553    3519 
   Size:       1553    1966
 
EarI CTCTTCn'nnn_

Cuts at:      0    1541    3519 
   Size:       1541    1978
 
EcoICRI GAG'CTC

Cuts at:      0    1229    3519 
   Size:       1229    2290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     766    1310    2981    3262    3384    3443    3519 
   Size:        566     200     544    1671     281     122      59      76

  Fragments arranged by size: 

                1671     566     544     281     200     122      76      59
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2334    3519 
   Size:       2334    1185
 
EcoRI G'AATT_C

Cuts at:      0     704    3519 
   Size:        704    2815
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      97     922    2899    3519 
   Size:         97     825    1977     620

  Fragments arranged by size: 

                1977     825     620      97
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       1      33      37      69    1192    1224    1594    1626 
   Size:          1      32       4      32    1123      32     370      32

Cuts at:   1626    1772    1804    3326    3358    3519 
   Size:        146      32    1522      32     161

  Fragments arranged by size: 

                1522    1123     370     161     146      32      32      32
                  32      32      32       4       1
 
HincII GTy'rAC

Cuts at:      0      45    3519 
   Size:         45    3474
 
HindIII A'AGCT_T

Cuts at:      0     199    3175    3519 
   Size:        199    2976     344

  Fragments arranged by size: 

                2976     344     199
 
KpnI G_GTAC'C

Cuts at:      0    1042    3519 
   Size:       1042    2477
 
MfeI C'AATT_G

Cuts at:      0     155    3519 
   Size:        155    3364
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     927    3519 
   Size:        102     825    2592

  Fragments arranged by size: 

                2592     825     102
 
MscI TGG'CCA

Cuts at:      0    1555    3519 
   Size:       1555    1964
 
MslI CAynn'nnrTG

Cuts at:      0    1387    1486    1702    3157    3519 
   Size:       1387      99     216    1455     362

  Fragments arranged by size: 

                1455    1387     362     216      99
 
MspA1I CmG'CkG

Cuts at:      0    1111    3519 
   Size:       1111    2408
 
NcoI C'CATG_G

Cuts at:      0    3152    3519 
   Size:       3152     367
 
NdeI CA'TA_TG

Cuts at:      0    1463    3519 
   Size:       1463    2056
 
NsiI A_TGCA'T

Cuts at:      0    1341    1937    3519 
   Size:       1341     596    1582

  Fragments arranged by size: 

                1582    1341     596
 
NspI r_CATG'y

Cuts at:      0    1386    1899    1939    3519 
   Size:       1386     513      40    1580

  Fragments arranged by size: 

                1580    1386     513      40
 
PciI A'CATG_T

Cuts at:      0    1382    1895    3519 
   Size:       1382     513    1624

  Fragments arranged by size: 

                1624    1382     513
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     492    3519 
   Size:        492    3027
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1450    1482    3519 
   Size:       1450      32    2037

  Fragments arranged by size: 

                2037    1450      32
 
PsiI TTA'TAA

Cuts at:      0    1010    3519 
   Size:       1010    2509
 
PvuII CAG'CTG

Cuts at:      0    1111    3519 
   Size:       1111    2408
 
SacI G_AGCT'C

Cuts at:      0    1231    3519 
   Size:       1231    2288
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1541    3519 
   Size:       1541    1978
 
ScaI AGT'ACT

Cuts at:      0     846    3519 
   Size:        846    2673
 
SfcI C'TryA_G

Cuts at:      0     188     368    1470    2225    3349    3519 
   Size:        188     180    1102     755    1124     170

  Fragments arranged by size: 

                1124    1102     755     188     180     170
 
SmlI C'TyrA_G

Cuts at:      0      82     103    1447    3519 
   Size:         82      21    1344    2072

  Fragments arranged by size: 

                2072    1344      82      21
 
SnaBI TAC'GTA

Cuts at:      0    2184    3519 
   Size:       2184    1335
 
SpeI A'CTAG_T

Cuts at:      0    2865    3519 
   Size:       2865     654
 
SphI G_CATG'C

Cuts at:      0    1939    3519 
   Size:       1939    1580
 
SspI AAT'ATT

Cuts at:      0    1065    2457    2968    3519 
   Size:       1065    1392     511     551

  Fragments arranged by size: 

                1392    1065     551     511
 
StuI AGG'CCT

Cuts at:      0     787    3519 
   Size:        787    2732
 
StyI C'CwwG_G

Cuts at:      0    3152    3519 
   Size:       3152     367
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     540     866    2102    3145    3519 
   Size:        540     326    1236    1043     374

  Fragments arranged by size: 

                1236    1043     540     374     326
 
TatI w'GTAC_w

Cuts at:      0     656     844    1203    3276    3519 
   Size:        656     188     359    2073     243

  Fragments arranged by size: 

                2073     656     359     243     188
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     815     847    2572    2604    3519 
   Size:        815      32    1725      32     915

  Fragments arranged by size: 

                1725     915     815      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      15    3519 
   Size:         15    3504
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2322    3519 
   Size:       2322    1197
 
XmnI GAAnn'nnTTC

Cuts at:      0    1730    3519 
   Size:       1730    1789
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AhdI    AlwNI     ApoI
     AseI     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI
     NspI     PciI    PflMI     PpiI     PsiI    PvuII     SacI     SapI
     ScaI     SfcI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AleI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BcgI     BglI Bme1580I    BmgBI     BmtI    BsaHI    BsaWI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BtgZI     ClaI    CspCI     DrdI     EagI
     EciI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SrfI
     SwaI     XbaI     XhoI     XmaI     ZraI
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