Restriction map for pF1KA1018
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BbeI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaI        BsiWI       
Bsp1407I    BspDI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          828  GTCTAC               GT^[AC][GT]AC
AgeI         1108  ACCGGT               A^CCGGT
Alw44I       2610  GTGCAC               G^TGCAC
ApaI         2808  GGGCCC               GGGCC^C
ApaLI        2610  GTGCAC               G^TGCAC
AspI         2855  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BsiEI        1737  CGGCCG               CG[GA][CT]^CG
BsmBI        2768  CGTCTC               CGTCTC(1/5)
BspHI        2670  TCATGA               T^CATGA
BspLU11I     2042  ACATGT               A^CATGT
BstXI        1580  CCAAAGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       2363  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2423  CCACGG               C^C[GA][CT]GG
EagI         1737  CGGCCG               C^GGCCG
EclXI        1737  CGGCCG               C^GGCCG
Eco52I       1737  CGGCCG               C^GGCCG
EcoRV        1048  GATATC               GAT^ATC
EcoT14I      1586  CCTTGG               C^C[AT][AT]GG
HaeII        2159  AGCGCC               [GA]GCGC^[CT]
HindIII        45  AAGCTT               A^AGCTT
NdeI          685  CATATG               CA^TATG
PflFI        2855  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2362  CCACAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1108  ACCGGT               A^CCGGT
RcaI         2670  TCATGA               T^CATGA
SacI         1885  GAGCTC               GAGCT^C
SexAI        2879  ACCTGGT              A^CC[AT]GGT
SfiI         2400  GGCCGGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          734  CCCGGG               CCC^GGG
SnaBI         487  TACGTA               TAC^GTA
StuI           34  AGGCCT               AGG^CCT
StyI         1586  CCTTGG               C^C[AT][AT]GG
Tth111I      2855  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2362  CCACAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2484  CCAGGGGATTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         734  CCCGGG               C^CCGGG
XmaI          734  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII       2205  TCCGGA               T^CCGGA
             2565  TCCGGA              
AfaI         1156  GTAC                 GT^AC
             1603  GTAC                
AvaI          734  CCCGGG               C^[CT]CG[GA]G
             2680  CCCGAG              
BamHI         216  GGATCC               G^GATCC
             2202  GGATCC              
BanII        1885  GAGCTC               G[GA]GC[CT]^C
             2808  GGGCCC              
BbrPI        2335  CACGTG               CAC^GTG
             2712  CACGTG              
BseAI        2205  TCCGGA               T^CCGGA
             2565  TCCGGA              
BsoBI         734  CCCGGG               C^[CT]CG[GA]G
             2680  CCCGAG              
BspEI        2205  TCCGGA               T^CCGGA
             2565  TCCGGA              
BspMI         114  ACCTGC               ACCTGC(4/8)
              225  GCAGGT              
BsrDI        1088  GCAATG               GCAATG(2/0)
             1852  GCAATG              
CfoI         2160  GCGC                 GCG^C
             2253  GCGC                
FauI         1637  CCCGC                CCCGC(4/6)
             2694  GCGGG               
HhaI         2160  GCGC                 GCG^C
             2253  GCGC                
HinP1I       2160  GCGC                 G^CGC
             2253  GCGC                
HincII       1659  GTTGAC               GT[CT]^[GA]AC
             1729  GTCAAC              
HindII       1659  GTTGAC               GT[CT]^[GA]AC
             1729  GTCAAC              
MroI         2205  TCCGGA               T^CCGGA
             2565  TCCGGA              
PleI         1666  GACTC                GAGTC(4/5)
             2263  GAGTC               
PmaCI        2335  CACGTG               CAC^GTG
             2712  CACGTG              
PmlI         2335  CACGTG               CAC^GTG
             2712  CACGTG              
RsaI         1156  GTAC                 GT^AC
             1603  GTAC                
SmlI         1159  CTTGAG               C^T[CT][GA]AG
             2647  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AcyI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BalI        BanI        BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpuAI       BsaAI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
Hin1I       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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